SH3GL2 ORF clone FHC06816

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC06816

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Clone info.

Species

human

Clone Name

pFN21AB6462 Sequence (with vector) Restriction Map

Product ID

FHC06816

Flexi Clone PID

FXC06816

ORF Sequence

DNA sequence (1056 bp)
Protein sequence (352 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464202]

Gene

KIBB6462

Symbol

SH3GL2

Alias : CNSA2, EEN-B1, SH3D2A, SH3P4

Description

SH3-domain GRB2-like 2

Original Clone ID

cs01062

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1056 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 352 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6483.1 1.5e-88 100.0 SH3GL2 CCDS10325.2 7e-60 68.1 SH3GL3 CCDS73772.1 1.9e-56 67.1 SH3GL3 CCDS32874.1 9.8e-51 71.1 SH3GL1 CCDS56076.1 2.9e-28 62.4 SH3GL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR013315 292 311 PR01887 Spectrin alpha chain IPR001452 293 303 PR00452 Src homology-3 domain IPR001452 307 322 PR00452 Src homology-3 domain IPR013315 312 326 PR01887 Spectrin alpha chain IPR001452 324 333 PR00452 Src homology-3 domain IPR013315 327 348 PR01887 Spectrin alpha chain IPR001452 335 347 PR00452 Src homology-3 domain HMMPfam IPR004148 6 241 PF03114 BAR IPR011511 295 346 PF07653 Variant SH3 HMMSmart IPR004148 5 242 SM00721 BAR IPR001452 293 348 SM00326 Src homology-3 domain ProfileScan IPR004148 18 249 PS51021 BAR IPR001452 290 349 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003017 352 604465 100.0 100.0 XP_011516307 386 604465 100.0 95.7 NP_003018 347 603362 68.1 100.0 XP_011520191 355 603362 67.1 96.0 NP_001288038 355 603362 67.1 96.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6462 Download

>KIBB6462  1056 bp
ATGTCGGTGGCCGGCCTCAAGAAGCAGTTCCATAAAGCCACTCAGAAAGTGAGTGAGAAG
GTTGGAGGAGCTGAAGGAACCAAGCTAGATGATGACTTCAAAGAGATGGAAAGGAAAGTG
GATGTCACCAGCAGGGCTGTGATGGAAATAATGACTAAAACAATTGAATACCTTCAACCC
AATCCAGCTTCCAGAGCTAAGCTCAGCATGATCAACACCATGTCAAAAATCCGTGGCCAG
GAGAAGGGGCCAGGCTATCCTCAGGCAGAGGCGCTGCTGGCAGAGGCCATGCTCAAATTT
GGAAGAGAGCTTGGAGATGATTGCAACTTTGGCCCAGCACTTGGTGAGGTCGGGGAGGCC
ATGCGGGAACTGTCGGAGGTCAAAGACTCTTTGGACATAGAAGTGAAGCAGAACTTCATT
GACCCTCTTCAGAATCTTCATGACAAAGATCTTAGGGAAATTCAACATCATCTAAAGAAG
TTGGAGGGTCGACGCCTGGATTTTGATTATAAGAAGAAACGACAAGGCAAGATTCCGGAT
GAAGAGCTTCGTCAAGCTCTAGAGAAATTTGATGAGTCTAAGGAAATTGCTGAGTCAAGC
ATGTTCAATCTCTTGGAGATGGATATTGAACAAGTGAGCCAGCTCTCTGCACTTGTGCAA
GCTCAGCTGGAGTACCACAAGCAGGCAGTCCAGATCCTGCAGCAAGTCACGGTCAGACTG
GAAGAAAGAATAAGACAGGCTTCATCTCAGCCTAGAAGGGAATATCAACCTAAACCACGA
ATGAGCCTGGAGTTTCCAACTGGAGACAGTACTCAGCCCAATGGGGGTCTCTCCCACACA
GGCACTCCCAAACCTTCAGGTGTCCAAATGGATCAGCCCTGCTGCCGAGCTCTGTACGAC
TTTGAACCTGAAAATGAAGGGGAGTTGGGATTTAAAGAGGGCGATATCATCACACTCACT
AACCAAATTGATGAGAACTGGTATGAGGGGATGCTGCATGGCCATTCAGGCTTCTTCCCC
ATCAATTATGTGGAAATTCTGGTTGCCCTGCCCCAT

ORF Protein

Cloned ORF protein sequence for pFN21AB6462 Download

>KIBB6462  352 aa
MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQP
NPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEA
MRELSEVKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPD
EELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRL
EERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSHTGTPKPSGVQMDQPCCRALYD
FEPENEGELGFKEGDIITLTNQIDENWYEGMLHGHSGFFPINYVEILVALPH

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6462 Download

>pFN21AB6462  5761 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGTGGCCGGCCTCAAGAAGCAGTTCCATAA
AGCCACTCAGAAAGTGAGTGAGAAGGTTGGAGGAGCTGAAGGAACCAAGCTAGATGATGA
CTTCAAAGAGATGGAAAGGAAAGTGGATGTCACCAGCAGGGCTGTGATGGAAATAATGAC
TAAAACAATTGAATACCTTCAACCCAATCCAGCTTCCAGAGCTAAGCTCAGCATGATCAA
CACCATGTCAAAAATCCGTGGCCAGGAGAAGGGGCCAGGCTATCCTCAGGCAGAGGCGCT
GCTGGCAGAGGCCATGCTCAAATTTGGAAGAGAGCTTGGAGATGATTGCAACTTTGGCCC
AGCACTTGGTGAGGTCGGGGAGGCCATGCGGGAACTGTCGGAGGTCAAAGACTCTTTGGA
CATAGAAGTGAAGCAGAACTTCATTGACCCTCTTCAGAATCTTCATGACAAAGATCTTAG
GGAAATTCAACATCATCTAAAGAAGTTGGAGGGTCGACGCCTGGATTTTGATTATAAGAA
GAAACGACAAGGCAAGATTCCGGATGAAGAGCTTCGTCAAGCTCTAGAGAAATTTGATGA
GTCTAAGGAAATTGCTGAGTCAAGCATGTTCAATCTCTTGGAGATGGATATTGAACAAGT
GAGCCAGCTCTCTGCACTTGTGCAAGCTCAGCTGGAGTACCACAAGCAGGCAGTCCAGAT
CCTGCAGCAAGTCACGGTCAGACTGGAAGAAAGAATAAGACAGGCTTCATCTCAGCCTAG
AAGGGAATATCAACCTAAACCACGAATGAGCCTGGAGTTTCCAACTGGAGACAGTACTCA
GCCCAATGGGGGTCTCTCCCACACAGGCACTCCCAAACCTTCAGGTGTCCAAATGGATCA
GCCCTGCTGCCGAGCTCTGTACGACTTTGAACCTGAAAATGAAGGGGAGTTGGGATTTAA
AGAGGGCGATATCATCACACTCACTAACCAAATTGATGAGAACTGGTATGAGGGGATGCT
GCATGGCCATTCAGGCTTCTTCCCCATCAATTATGTGGAAATTCTGGTTGCCCTGCCCCA
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T