(Circular) (Six-base) MAPSORT of: pFN21AB6462.seq Check: 6379 from: 1 to: 5761 pFN21AB6462 5761 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2494 3104 1937 Size: 557 610 4594 Fragments arranged by size: 4594 610 557 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2417 2844 3824 5208 5476 1632 Size: 465 320 427 980 1384 268 1917 Fragments arranged by size: 1917 1384 980 465 427 320 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2701 3504 5076 2701 Size: 803 1572 3386 Fragments arranged by size: 3386 1572 803 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2300 2463 2570 3039 3070 3296 3448 5557 Size: 807 163 107 469 31 226 152 2109 Cuts at: 5557 1493 Size: 1697 Fragments arranged by size: 2109 1697 807 469 226 163 152 107 31 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 4537 618 Size: 325 217 627 1295 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 217 BanII G_rGCy'C Cuts at: 1391 2896 3080 1391 Size: 1505 184 4072 Fragments arranged by size: 4072 1505 184 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2214 1548 Size: 666 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3012 4372 136 Size: 107 122 71 631 605 1340 1360 1525 Fragments arranged by size: 1525 1360 1340 631 605 122 107 71 BglII A'GATC_T Cuts at: 2452 5756 2452 Size: 3304 2457 BlpI GC'TnA_GC Cuts at: 2202 2207 2667 3178 2202 Size: 5 460 511 4785 Fragments arranged by size: 4785 511 460 5 Bme1580I G_kGCm'C Cuts at: 1790 3822 4978 1790 Size: 2032 1156 2573 Fragments arranged by size: 2573 2032 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2222 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2693 2813 2826 4421 1239 Size: 1454 120 13 1595 2579 Fragments arranged by size: 2579 1595 1454 120 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 3886 4751 5049 2006 Size: 1880 865 298 2718 Fragments arranged by size: 2718 1880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2837 4424 882 Size: 617 1338 1587 2219 Fragments arranged by size: 2219 1587 1338 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2497 3568 3950 4585 Size: 53 83 186 936 964 1071 382 635 Cuts at: 4585 275 Size: 1451 Fragments arranged by size: 1451 1071 964 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2539 4194 4592 4866 5013 5424 2539 Size: 1655 398 274 147 411 2876 Fragments arranged by size: 2876 1655 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2833 2863 1178 Size: 30 165 30 1430 30 4076 Fragments arranged by size: 4076 1430 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 1186 Size: 899 4862 BseYI C'CCAG_C Cuts at: 2338 4964 5460 2338 Size: 2626 496 2639 Fragments arranged by size: 2639 2626 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 1163 Size: 573 901 4287 Fragments arranged by size: 4287 901 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2896 3822 3907 4978 2896 Size: 926 85 1071 3679 Fragments arranged by size: 3679 1071 926 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2896 3080 3822 3907 4978 1391 Size: 399 1106 184 742 85 1071 2174 Fragments arranged by size: 2174 1106 1071 742 399 184 85 BspEI T'CCGG_A Cuts at: 2539 5424 2539 Size: 2885 2876 BspHI T'CATG_A Cuts at: 2443 3650 5380 2443 Size: 1207 1730 2824 Fragments arranged by size: 2824 1730 1207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 4405 4592 1700 Size: 110 205 2390 187 2869 Fragments arranged by size: 2869 2390 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2452 2697 3091 3458 3843 3860 5312 5398 Size: 480 245 394 367 385 17 1452 86 Cuts at: 5398 5410 5756 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 480 394 385 367 346 245 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2265 2265 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2236 513 Size: 552 1171 4038 Fragments arranged by size: 4038 1171 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 2938 3065 2938 Size: 127 5634 DraIII CAC_nnn'GTG Cuts at: 2348 2348 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2239 3004 Size: 54 1425 50 275 18 183 226 765 Cuts at: 3004 3208 4097 5726 8 Size: 204 889 1629 43 Fragments arranged by size: 1629 1425 889 765 275 226 204 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2302 2436 2541 3691 1353 Size: 949 134 105 1150 3423 Fragments arranged by size: 3423 1150 949 134 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2894 2894 Size: 5761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2417 2693 2813 2826 2844 3824 Size: 393 465 320 276 120 13 18 980 Cuts at: 3824 4421 5208 5476 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 980 787 597 465 393 320 276 268 120 18 13 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 EcoRV GAT'ATC Cuts at: 2950 2950 Size: 5761 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 2760 2434 Size: 326 5435 FseI GG_CCGG'CC Cuts at: 2019 2019 Size: 5761 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2279 4908 1678 Size: 601 2629 2531 Fragments arranged by size: 2629 2531 601 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373 Size: 32 135 32 2830 32 42 32 2626 Fragments arranged by size: 2830 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2495 3105 3351 669 Size: 988 281 557 610 246 3079 Fragments arranged by size: 3079 988 610 557 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 2166 3360 2166 Size: 1194 4567 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 2358 2466 2825 4875 5059 1305 Size: 742 311 108 359 2050 184 2007 Fragments arranged by size: 2050 2007 742 359 311 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 3006 10 Size: 54 1425 343 409 765 2765 Fragments arranged by size: 2765 1425 765 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2405 3719 4078 4237 518 Size: 648 843 396 1314 359 159 2042 Fragments arranged by size: 2042 1314 843 648 396 359 159 MspA1I CmG'CkG Cuts at: 1606 2671 3177 3854 5002 5247 1606 Size: 1065 506 677 1148 245 2120 Fragments arranged by size: 2120 1148 1065 677 506 245 NaeI GCC'GGC Cuts at: 2017 2017 Size: 5761 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5209 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NgoMIV G'CCGG_C Cuts at: 2015 2015 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2608 3119 4664 1132 Size: 97 1379 511 1545 2229 Fragments arranged by size: 2229 1545 1379 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 2345 1108 Size: 1237 4524 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 2514 3331 2514 Size: 817 4944 PstI C_TGCA'G Cuts at: 830 2706 3113 830 Size: 1876 407 3478 Fragments arranged by size: 3478 1876 407 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2671 2671 Size: 5761 SacI G_AGCT'C Cuts at: 2896 2896 Size: 5761 SalI G'TCGA_C Cuts at: 1936 2493 3103 1936 Size: 557 610 4594 Fragments arranged by size: 4594 610 557 SapI GCTCTTCn'nnn_ Cuts at: 2541 2541 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 2815 4009 5721 2815 Size: 1194 1712 2855 Fragments arranged by size: 2855 1712 1194 SfcI C'TryA_G Cuts at: 826 1045 2702 3109 4244 4925 5116 826 Size: 219 1657 407 1135 681 191 1471 Fragments arranged by size: 1657 1471 1135 681 407 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 3865 4766 5028 820 Size: 197 926 78 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 3200 513 Size: 552 2135 3074 Fragments arranged by size: 3074 2135 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2813 4007 5719 Size: 274 80 33 51 1443 836 1194 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1194 836 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2833 2865 5721 5753 2833 Size: 32 2856 32 2841 Fragments arranged by size: 2856 2841 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 2563 3097 2563 Size: 534 5227 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3031 3031 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 2030 2175 2550 3890 2030 Size: 145 375 1340 3901 Fragments arranged by size: 3901 1340 375 145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI