KRT35 ORF clone FHC05781

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05781

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Clone info.

Species

human

Clone Name

pFN21AB7222 Sequence (with vector) Restriction Map

Product ID

FHC05781

Flexi Clone PID

FXC05781

ORF Sequence

DNA sequence (1365 bp)
Protein sequence (455 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463814]

Gene

KIBB7222

Symbol

KRT35

Alias : HA5, Ha-5, K35, KRTHA5, hHa5

Description

keratin 35, type I

Original Clone ID

oc00438

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1365 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 455 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11394.2 7.6e-116 100.0 KRT35 CCDS11395.1 6.2e-75 70.1 KRT36 CCDS11393.1 9.8e-71 66.8 KRT32 CCDS11391.1 1.1e-69 71.9 KRT31 CCDS11388.1 4.1e-66 70.3 KRT33A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR002957 175 188 PR01248 Keratin IPR002957 196 219 PR01248 Keratin IPR002957 250 270 PR01248 Keratin IPR002957 322 337 PR01248 Keratin IPR002957 348 374 PR01248 Keratin HMMPfam IPR016044 96 407 PF00038 Filament
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002271 455 602764 100.0 100.0 XP_011523096 455 602764 100.0 100.0 NP_003762 467 604540 70.1 97.8 NP_002269 448 602760 66.8 100.0 NP_002268 416 601077 71.9 88.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7222 Download

>KIBB7222  1365 bp
ATGGCTTCCAAATGCCTCAAGGCCGGCTTCTCTTCTGGGTCTCTCAAGAGCCCAGGAGGG
GCCAGTGGGGGCTCCACTCGTGTGTCCGCAATGTACTCCAGCAGCTCTTGCAAGCTTCCA
AGTCTCTCCCCTGTGGCCAGAAGTTTCTCTGCCTGCTCAGTGGGTCTGGGCAGAAGCAGC
TACAGGGCCACCAGCTGCCTCCCTGCTCTCTGCCTCCCTGCTGGAGGCTTCGCTACCAGC
TACAGTGGGGGTGGGGGCTGGTTTGGGGAGGGCATCCTCACTGGCAATGAGAAGGAGACC
ATGCAATCCCTGAACGACCGCCTGGCCGGCTACCTGGAGAAGGTGCGTCAGCTGGAGCAG
GAGAACGCCAGCCTGGAGAGCCGCATCCGTGAGTGGTGTGAGCAGCAGGTCCCCTACATG
TGCCCTGACTACCAGTCCTACTTCCGGACCATCGAGGAGCTCCAGAAGAAGACTCTATGC
AGCAAGGCTGAGAATGCCAGGCTGGTGGTGGAGATTGACAATGCCAAATTGGCTGCAGAT
GACTTCAGGACCAAGTATGAGACGGAGGTGTCCCTGCGGCAGCTGGTGGAGTCAGACATC
AACGGCCTGCGCAGGATCCTGGATGACCTGACCCTGTGCAAGTCTGACCTGGAGGCCCAG
GTGGAGTCCCTGAAGGAGGAGCTGCTCTGCCTGAAGAAGAACCATGAGGAGGAAGTGAAC
TCACTGCGCTGCCAACTTGGTGACCGCCTCAATGTTGAGGTGGACGCTGCCCCACCTGTT
GACCTGAACCGAGTTCTGGAGGAGATGAGGTGCCAGTATGAAACCCTGGTGGAGAATAAC
CGCCGGGATGCTGAAGACTGGTTGGACACCCAGAGTGAGGAGCTGAACCAGCAGGTGGTG
TCCAGCTCAGAGCAGTTGCAGTCCTGCCAGGCAGAGATCATCGAGCTGAGACGCACGGTC
AACGCCCTGGAGATTGAGCTGCAGGCTCAGCACAGCATGAGAGATGCTTTGGAATCCACC
CTGGCAGAGACGGAGGCCCGCTATAGCTCCCAGCTGGCCCAGATGCAGTGCATGATCACC
AACGTGGAGGCCCAGCTGGCCGAGATCCGGGCTGACCTGGAGCGGCAGAACCAGGAGTAC
CAGGTGCTGCTGGACGTCCGGGCCCGGCTGGAGTGTGAGATCAACACGTACCGGGGCCTG
CTGGAGAGTGAGGACAGCAAGCTCCCCTGTAACCCATGTGCACCTGACTACTCACCCTCC
AAGTCATGCCTTCCCTGTCTTCCTGCGGCCTCCTGCGGTCCTAGTGCAGCCCGCACAAAC
TGCAGCCCCCGCCCCATTTGTGTGCCCTGCCCAGGGGGTCGGTTC

ORF Protein

Cloned ORF protein sequence for pFN21AB7222 Download

>KIBB7222  455 aa
MASKCLKAGFSSGSLKSPGGASGGSTRVSAMYSSSSCKLPSLSPVARSFSACSVGLGRSS
YRATSCLPALCLPAGGFATSYSGGGGWFGEGILTGNEKETMQSLNDRLAGYLEKVRQLEQ
ENASLESRIREWCEQQVPYMCPDYQSYFRTIEELQKKTLCSKAENARLVVEIDNAKLAAD
DFRTKYETEVSLRQLVESDINGLRRILDDLTLCKSDLEAQVESLKEELLCLKKNHEEEVN
SLRCQLGDRLNVEVDAAPPVDLNRVLEEMRCQYETLVENNRRDAEDWLDTQSEELNQQVV
SSSEQLQSCQAEIIELRRTVNALEIELQAQHSMRDALESTLAETEARYSSQLAQMQCMIT
NVEAQLAEIRADLERQNQEYQVLLDVRARLECEINTYRGLLESEDSKLPCNPCAPDYSPS
KSCLPCLPAASCGPSAARTNCSPRPICVPCPGGRF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7222 Download

>pFN21AB7222  6070 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTCCAAATGCCTCAAGGCCGGCTTCTCTTC
TGGGTCTCTCAAGAGCCCAGGAGGGGCCAGTGGGGGCTCCACTCGTGTGTCCGCAATGTA
CTCCAGCAGCTCTTGCAAGCTTCCAAGTCTCTCCCCTGTGGCCAGAAGTTTCTCTGCCTG
CTCAGTGGGTCTGGGCAGAAGCAGCTACAGGGCCACCAGCTGCCTCCCTGCTCTCTGCCT
CCCTGCTGGAGGCTTCGCTACCAGCTACAGTGGGGGTGGGGGCTGGTTTGGGGAGGGCAT
CCTCACTGGCAATGAGAAGGAGACCATGCAATCCCTGAACGACCGCCTGGCCGGCTACCT
GGAGAAGGTGCGTCAGCTGGAGCAGGAGAACGCCAGCCTGGAGAGCCGCATCCGTGAGTG
GTGTGAGCAGCAGGTCCCCTACATGTGCCCTGACTACCAGTCCTACTTCCGGACCATCGA
GGAGCTCCAGAAGAAGACTCTATGCAGCAAGGCTGAGAATGCCAGGCTGGTGGTGGAGAT
TGACAATGCCAAATTGGCTGCAGATGACTTCAGGACCAAGTATGAGACGGAGGTGTCCCT
GCGGCAGCTGGTGGAGTCAGACATCAACGGCCTGCGCAGGATCCTGGATGACCTGACCCT
GTGCAAGTCTGACCTGGAGGCCCAGGTGGAGTCCCTGAAGGAGGAGCTGCTCTGCCTGAA
GAAGAACCATGAGGAGGAAGTGAACTCACTGCGCTGCCAACTTGGTGACCGCCTCAATGT
TGAGGTGGACGCTGCCCCACCTGTTGACCTGAACCGAGTTCTGGAGGAGATGAGGTGCCA
GTATGAAACCCTGGTGGAGAATAACCGCCGGGATGCTGAAGACTGGTTGGACACCCAGAG
TGAGGAGCTGAACCAGCAGGTGGTGTCCAGCTCAGAGCAGTTGCAGTCCTGCCAGGCAGA
GATCATCGAGCTGAGACGCACGGTCAACGCCCTGGAGATTGAGCTGCAGGCTCAGCACAG
CATGAGAGATGCTTTGGAATCCACCCTGGCAGAGACGGAGGCCCGCTATAGCTCCCAGCT
GGCCCAGATGCAGTGCATGATCACCAACGTGGAGGCCCAGCTGGCCGAGATCCGGGCTGA
CCTGGAGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTCCGGGCCCGGCTGGAGTG
TGAGATCAACACGTACCGGGGCCTGCTGGAGAGTGAGGACAGCAAGCTCCCCTGTAACCC
ATGTGCACCTGACTACTCACCCTCCAAGTCATGCCTTCCCTGTCTTCCTGCGGCCTCCTG
CGGTCCTAGTGCAGCCCGCACAAACTGCAGCCCCCGCCCCATTTGTGTGCCCTGCCCAGG
GGGTCGGTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT