(Circular) (Six-base) MAPSORT of: pFN21AB7222.seq Check: 521 from: 1 to: 6070 pFN21AB7222 6070 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2887 2887 Size: 6070 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3162 3880 278 Size: 53 83 186 936 1626 718 2468 Fragments arranged by size: 2468 1626 936 718 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2696 2717 2877 4133 5517 5785 1632 Size: 901 163 21 160 1256 1384 268 1917 Fragments arranged by size: 1917 1384 1256 901 268 163 160 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2421 3189 4969 5946 1203 Size: 1218 768 1780 977 1327 Fragments arranged by size: 1780 1327 1218 977 768 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 2437 2960 4799 2437 Size: 523 1839 3708 Fragments arranged by size: 3708 1839 523 AleI CACnn'nnGTG Cuts at: 1166 2084 3086 1166 Size: 918 1002 4150 Fragments arranged by size: 4150 1002 918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 3151 3813 5385 3151 Size: 662 1572 3836 Fragments arranged by size: 3836 1572 662 ApaI G_GGCC'C Cuts at: 3169 3169 Size: 6070 ApaLI G'TGCA_C Cuts at: 3243 4127 5283 3243 Size: 884 1156 4030 Fragments arranged by size: 4030 1156 884 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 3395 1870 Size: 73 1452 4545 Fragments arranged by size: 4545 1452 73 BamHI G'GATC_C Cuts at: 2619 3400 3767 2619 Size: 781 367 4922 Fragments arranged by size: 4922 781 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2814 3391 4846 618 Size: 325 217 627 1027 577 1455 1842 Fragments arranged by size: 1842 1455 1027 627 577 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2078 2466 3169 3389 1391 Size: 666 21 388 703 220 4072 Fragments arranged by size: 4072 703 666 388 220 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2480 2865 3275 928 Size: 971 581 385 410 3723 Fragments arranged by size: 3723 971 581 410 385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 3078 1548 Size: 1530 4540 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2331 2534 3101 Size: 107 122 71 631 605 659 203 567 Cuts at: 3101 4681 136 Size: 1580 1525 Fragments arranged by size: 1580 1525 659 631 605 567 203 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 2991 3487 2991 Size: 496 5574 Bme1580I G_kGCm'C Cuts at: 1790 2429 3169 3247 3351 4131 5287 1790 Size: 639 740 78 104 780 1156 2573 Fragments arranged by size: 2573 1156 780 740 639 104 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2086 2247 2360 2378 2399 2450 2675 2822 Size: 847 161 113 18 21 51 225 147 Cuts at: 2822 2993 3143 3194 3227 4730 1239 Size: 171 150 51 33 1503 2579 Fragments arranged by size: 2579 1503 847 225 171 161 150 147 113 51 51 33 21 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 2033 4195 5060 5358 2006 Size: 27 2162 865 298 2718 Fragments arranged by size: 2718 2162 865 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2049 2294 4733 882 Size: 617 550 245 2439 2219 Fragments arranged by size: 2439 2219 617 550 245 BsaAI yAC'GTr Cuts at: 493 1204 3192 3861 493 Size: 711 1988 669 2702 Fragments arranged by size: 2702 1988 711 669 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3159 3877 4259 4894 Size: 53 83 186 936 1626 718 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1626 1451 936 718 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 4503 4901 5175 5322 5733 2448 Size: 2055 398 274 147 411 2785 Fragments arranged by size: 2785 2055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2798 2828 3245 3275 1178 Size: 30 165 30 1395 30 417 30 3973 Fragments arranged by size: 3973 1395 417 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 2696 2726 2819 2897 1186 Size: 1288 222 30 93 78 4359 Fragments arranged by size: 4359 1288 222 93 78 30 BseYI C'CCAG_C Cuts at: 3054 3096 5273 5769 3054 Size: 42 2177 496 3355 Fragments arranged by size: 3355 2177 496 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3330 1163 Size: 573 1594 3903 Fragments arranged by size: 3903 1594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2323 3520 4281 5309 1540 Size: 275 186 322 1197 761 1028 2301 Fragments arranged by size: 2301 1197 1028 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 3247 4131 4216 5287 2466 Size: 781 884 85 1071 3249 Fragments arranged by size: 3249 1071 884 781 85 BsmI GAATG_Cn' Cuts at: 2503 3581 3674 2503 Size: 1078 93 4899 Fragments arranged by size: 4899 1078 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2558 2947 3026 2558 Size: 389 79 5602 Fragments arranged by size: 5602 389 79 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2078 2429 2466 3169 3247 3351 Size: 399 267 21 351 37 703 78 104 Cuts at: 3351 3389 4131 4216 5287 1391 Size: 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 399 351 267 104 85 78 38 37 21 BspEI T'CCGG_A Cuts at: 2448 5733 2448 Size: 3285 2785 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2401 2887 3425 844 Size: 1557 486 538 3489 Fragments arranged by size: 3489 1557 538 486 BsrBI CCG'CTC Cuts at: 1394 1823 3127 3957 1394 Size: 429 1304 830 3507 Fragments arranged by size: 3507 1304 830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2100 2296 4565 4739 65 Size: 1183 852 196 2269 174 1396 Fragments arranged by size: 2269 1396 1183 852 196 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2330 4714 4901 1700 Size: 110 217 303 2384 187 2869 Fragments arranged by size: 2869 2384 303 217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2082 4130 5142 2082 Size: 2048 1012 3010 Fragments arranged by size: 3010 2048 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2112 3324 794 Size: 1318 1212 3540 Fragments arranged by size: 3540 1318 1212 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 2744 1184 Size: 1560 4510 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2619 3108 3400 3767 4152 4169 5621 5707 Size: 647 489 292 367 385 17 1452 86 Cuts at: 5707 5719 6065 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 647 489 385 367 346 292 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4579 Fragments arranged by size: 4579 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 2726 3077 3586 4380 4400 2726 Size: 351 509 794 20 4396 Fragments arranged by size: 4396 794 509 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2572 5077 809 Size: 507 1256 2505 1802 Fragments arranged by size: 2505 1802 1256 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2328 3102 Size: 54 1425 50 275 18 309 189 774 Cuts at: 3102 3517 4406 6035 8 Size: 415 889 1629 43 Fragments arranged by size: 1629 1425 889 774 415 309 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2041 4000 1353 Size: 688 1959 3423 Fragments arranged by size: 3423 1959 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2464 2464 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2247 2360 2378 2399 2450 2533 Size: 393 454 161 113 18 21 51 83 Cuts at: 2533 2675 2696 2717 2822 2877 2993 3143 3194 Size: 142 21 21 105 55 116 150 51 Cuts at: 3194 3227 4133 4730 5517 5785 1239 Size: 33 906 597 787 268 1524 Fragments arranged by size: 1524 906 787 597 454 393 268 161 150 142 116 113 105 83 55 51 51 33 21 21 21 18 EcoNI CCTnn'n_nnAGG Cuts at: 2226 2342 2678 2226 Size: 116 336 5618 Fragments arranged by size: 5618 336 116 EcoO109I rG'GnC_Cy Cuts at: 2413 3199 2413 Size: 786 5284 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 6070 FspI TGC'GCA Cuts at: 2615 4576 2615 Size: 1961 4109 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 2946 2978 4711 Size: 32 135 32 851 32 491 32 1733 Cuts at: 4711 4743 4785 4817 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1733 851 491 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2785 2965 3414 3660 669 Size: 988 281 847 180 449 246 3079 Fragments arranged by size: 3079 988 847 449 281 246 180 HindIII A'AGCT_T Cuts at: 748 2117 748 Size: 1369 4701 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2847 5184 5368 1305 Size: 1542 2337 184 2007 Fragments arranged by size: 2337 2007 1542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 10 Size: 54 1425 343 309 3939 Fragments arranged by size: 3939 1425 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 3086 3344 4028 4387 4546 518 Size: 648 918 1002 258 684 359 159 2042 Fragments arranged by size: 2042 1002 918 684 648 359 258 159 MspA1I CmG'CkG Cuts at: 1606 2199 2356 2587 3058 3100 3486 4163 5311 Size: 593 157 231 471 42 386 677 1148 Cuts at: 5311 5556 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 593 471 386 245 231 157 42 NaeI GCC'GGC Cuts at: 2029 2332 2029 Size: 303 5767 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4579 Fragments arranged by size: 4579 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NgoMIV G'CCGG_C Cuts at: 2027 2330 2027 Size: 303 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2425 3428 4973 1132 Size: 97 1196 1003 1545 2229 Fragments arranged by size: 2229 1545 1196 1003 97 PasI CC'CwG_GG Cuts at: 1348 3356 1348 Size: 2008 4062 PciI A'CATG_T Cuts at: 2421 4969 2421 Size: 2548 3522 PflMI CCAn_nnn'nTGG Cuts at: 1108 2899 1108 Size: 1791 4279 PfoI T'CCnGG_A Cuts at: 2622 2622 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2413 2413 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PspOMI G'GGCC_C Cuts at: 3165 3165 Size: 6070 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3125 3157 3125 Size: 32 6038 PstI C_TGCA'G Cuts at: 830 2542 2988 3329 3422 830 Size: 1712 446 341 93 3478 Fragments arranged by size: 3478 1712 446 341 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 PvuII CAG'CTG Cuts at: 2199 2356 2587 3058 3100 2199 Size: 157 231 471 42 5169 Fragments arranged by size: 5169 471 231 157 42 SacI G_AGCT'C Cuts at: 2466 2466 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SexAI A'CCwGG_T Cuts at: 3144 3144 Size: 6070 SfcI C'TryA_G Cuts at: 826 1045 2185 2245 2538 2984 3046 3325 3418 Size: 219 1140 60 293 446 62 279 93 Cuts at: 3418 4553 5234 5425 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1140 1135 681 446 293 279 219 191 93 62 60 SfiI GGCCn_nnn'nGGCC Cuts at: 3101 3101 Size: 6070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2048 4174 5075 5337 820 Size: 197 926 78 27 2126 901 262 1553 Fragments arranged by size: 2126 1553 926 901 262 197 78 27 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2004 3509 513 Size: 552 939 1505 3074 Fragments arranged by size: 3074 1505 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 4097 4114 4267 4452 4897 325 Size: 1255 1308 1209 17 153 185 445 1498 Fragments arranged by size: 1498 1308 1255 1209 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 4316 6028 Size: 274 80 33 51 1443 120 2219 1712 Cuts at: 6028 96 Size: 138 Fragments arranged by size: 2219 1712 1443 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2798 2830 6030 6062 2798 Size: 32 3200 32 2806 Fragments arranged by size: 3200 2806 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2509 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3160 3878 276 Size: 53 83 186 936 1626 718 2468 Fragments arranged by size: 2468 1626 936 718 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SapI SfoI SrfI SwaI