EFCAB14 ORF clone FHC05606

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC05606

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Clone info.

Species

human

Clone Name

pFN21AA0494 Sequence (with vector) Restriction Map

Product ID

FHC05606

Flexi Clone PID

FXC05606

ORF Sequence

DNA sequence (1485 bp)
Protein sequence (495 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527169]

Gene

KIAA0494

Symbol

EFCAB14

Alias : KIAA0494

Description

EF-hand calcium binding domain 14

Original Clone ID

hh00201

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1485 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 495 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30706.1 4.2e-152 100.0 EFCAB14 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition ProfileScan IPR018249 434 469 PS50222 EF-HAND 2 IPR018249 471 495 PS50222 EF-HAND 2
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 71 ICYPLCGFVILAACVVACVGLVW 93 PRIMARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0494 Download

>KIAA0494  1485 bp
ATGAAAAAGCGCAAAGAGCTCAATGCATTGATTGGTTTGGCTGGGGACAGCCGGAGAAAG
AAGCCCAAGAAAGGCCCAAGCAGTCACCGCCTGCTTCGCACTGAGCCTCCCGACTCAGAC
TCTGAGTCCAGCTCCGAAGAGGAAGAGGAATTCGGTGTGGTTGGAAATCGCTCTCGCTTT
GCCAAGGGAGACTATTTACGATGCTGCAAGATCTGTTATCCGCTCTGTGGTTTTGTCATC
CTTGCTGCCTGTGTTGTGGCCTGTGTTGGCTTGGTGTGGATGCAGGTTGCTCTCAAGGAG
GATCTGGATGCCCTCAAGGAAAAATTTCGAACAATGGAATCTAATCAGAAAAGCTCATTC
CAAGAAATCCCCAAACTTAATGAAGAACTACTCAGCAAGCAAAAACAACTTGAGAAGATT
GAATCTGGAGAGATGGGTTTGAACAAAGTCTGGATAAACATCACAGAAATGAATAAGCAG
ATTTCTCTGTTGACTTCTGCAGTGAACCACCTCAAAGCCAATGTTAAGTCAGCTGCAGAC
TTGATTAGCCTGCCTACCACTGTAGAGGGACTTCAGAAGAGTGTAGCTTCCATTGGCAAT
ACTTTAAACAGCGTCCATCTTGCTGTGGAAGCACTACAGAAAACTGTGGATGAACACAAG
AAAACGATGGAATTACTGCAGAGTGATATGAATCAGCACTTCTTGAAGGAGACTCCTGGA
AGCAACCAGATCATTCCGTCACCTTCAGCCACATCAGAACTTGACAATAAAACCCACAGT
GAGAATTTGAAACAGGATATCCTGTACCTTCACAACTCTTTAGAGGAGGTAAACAGTGCC
CTAGTGGGGTACCAGAGACAGAATGATCTTAAACTCGAGGGAATGAACGAGACAGTCAGT
AATCTTACCCAGAGAGTCAACCTGATAGAAAGCGATGTGGTTGCTATGAGCAAGGTAGAA
AAGAAAGCAAACCTGTCCTTCAGCATGATGGGTGATAGATCTGCCACTCTGAAAAGACAG
TCTTTGGATCAAGTCACCAACAGAACAGATACAGTAAAAATCCAAAGCATAAAGAAAGAA
GATAGTTCAAATTCTCAGGTATCCAAGCTAAGAGAGAAACTCCAGCTGATCAGTGCTCTT
ACAAACAAACCTGAGAGCAACAGGCCTCCAGAGACCGCCGATGAAGAGCAAGTAGAGAGT
TTCACATCAAAGCCATCAGCATTGCCAAAATTTTCACAGTTTCTTGGAGACCCAGTTGAG
AAAGCTGCCCAACTAAGACCTATCTCCCTACCAGGAGTTTCTAGCACTGAAGATCTTCAG
GATTTATTCCGCAAGACTGGCCAGGACGTGGATGGGAAGCTGACCTACCAGGAAATCTGG
ACCTCCCTAGGTTCTGCTATGCCAGAACCAGAGAGCTTGAGAGCATTTGATTCCGATGGA
GATGGAAGATACTCATTCCTGGAGCTAAGGGTAGCTTTAGGTATC

ORF Protein

Cloned ORF protein sequence for pFN21AA0494 Download

>KIAA0494  495 aa
MKKRKELNALIGLAGDSRRKKPKKGPSSHRLLRTEPPDSDSESSSEEEEEFGVVGNRSRF
AKGDYLRCCKICYPLCGFVILAACVVACVGLVWMQVALKEDLDALKEKFRTMESNQKSSF
QEIPKLNEELLSKQKQLEKIESGEMGLNKVWINITEMNKQISLLTSAVNHLKANVKSAAD
LISLPTTVEGLQKSVASIGNTLNSVHLAVEALQKTVDEHKKTMELLQSDMNQHFLKETPG
SNQIIPSPSATSELDNKTHSENLKQDILYLHNSLEEVNSALVGYQRQNDLKLEGMNETVS
NLTQRVNLIESDVVAMSKVEKKANLSFSMMGDRSATLKRQSLDQVTNRTDTVKIQSIKKE
DSSNSQVSKLREKLQLISALTNKPESNRPPETADEEQVESFTSKPSALPKFSQFLGDPVE
KAAQLRPISLPGVSSTEDLQDLFRKTGQDVDGKLTYQEIWTSLGSAMPEPESLRAFDSDG
DGRYSFLELRVALGI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0494 Download

>pFN21AA0494  6190 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAAAAGCGCAAAGAGCTCAATGCATTGATTGG
TTTGGCTGGGGACAGCCGGAGAAAGAAGCCCAAGAAAGGCCCAAGCAGTCACCGCCTGCT
TCGCACTGAGCCTCCCGACTCAGACTCTGAGTCCAGCTCCGAAGAGGAAGAGGAATTCGG
TGTGGTTGGAAATCGCTCTCGCTTTGCCAAGGGAGACTATTTACGATGCTGCAAGATCTG
TTATCCGCTCTGTGGTTTTGTCATCCTTGCTGCCTGTGTTGTGGCCTGTGTTGGCTTGGT
GTGGATGCAGGTTGCTCTCAAGGAGGATCTGGATGCCCTCAAGGAAAAATTTCGAACAAT
GGAATCTAATCAGAAAAGCTCATTCCAAGAAATCCCCAAACTTAATGAAGAACTACTCAG
CAAGCAAAAACAACTTGAGAAGATTGAATCTGGAGAGATGGGTTTGAACAAAGTCTGGAT
AAACATCACAGAAATGAATAAGCAGATTTCTCTGTTGACTTCTGCAGTGAACCACCTCAA
AGCCAATGTTAAGTCAGCTGCAGACTTGATTAGCCTGCCTACCACTGTAGAGGGACTTCA
GAAGAGTGTAGCTTCCATTGGCAATACTTTAAACAGCGTCCATCTTGCTGTGGAAGCACT
ACAGAAAACTGTGGATGAACACAAGAAAACGATGGAATTACTGCAGAGTGATATGAATCA
GCACTTCTTGAAGGAGACTCCTGGAAGCAACCAGATCATTCCGTCACCTTCAGCCACATC
AGAACTTGACAATAAAACCCACAGTGAGAATTTGAAACAGGATATCCTGTACCTTCACAA
CTCTTTAGAGGAGGTAAACAGTGCCCTAGTGGGGTACCAGAGACAGAATGATCTTAAACT
CGAGGGAATGAACGAGACAGTCAGTAATCTTACCCAGAGAGTCAACCTGATAGAAAGCGA
TGTGGTTGCTATGAGCAAGGTAGAAAAGAAAGCAAACCTGTCCTTCAGCATGATGGGTGA
TAGATCTGCCACTCTGAAAAGACAGTCTTTGGATCAAGTCACCAACAGAACAGATACAGT
AAAAATCCAAAGCATAAAGAAAGAAGATAGTTCAAATTCTCAGGTATCCAAGCTAAGAGA
GAAACTCCAGCTGATCAGTGCTCTTACAAACAAACCTGAGAGCAACAGGCCTCCAGAGAC
CGCCGATGAAGAGCAAGTAGAGAGTTTCACATCAAAGCCATCAGCATTGCCAAAATTTTC
ACAGTTTCTTGGAGACCCAGTTGAGAAAGCTGCCCAACTAAGACCTATCTCCCTACCAGG
AGTTTCTAGCACTGAAGATCTTCAGGATTTATTCCGCAAGACTGGCCAGGACGTGGATGG
GAAGCTGACCTACCAGGAAATCTGGACCTCCCTAGGTTCTGCTATGCCAGAACCAGAGAG
CTTGAGAGCATTTGATTCCGATGGAGATGGAAGATACTCATTCCTGGAGCTAAGGGTAGC
TTTAGGTATCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT