(Circular) (Six-base) MAPSORT of: pFN21AA0494.seq Check: 5237 from: 1 to: 6190 pFN21AA0494 6190 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 2853 3511 2853 Size: 658 5532 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 2733 2968 3305 3333 4253 5637 5905 Size: 929 172 235 337 28 920 1384 268 Cuts at: 5905 1632 Size: 1917 Fragments arranged by size: 1917 1384 929 920 337 268 235 172 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 5089 6066 1203 Size: 3886 977 1327 Fragments arranged by size: 3886 1327 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 2128 4919 2128 Size: 2791 3399 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AlwNI CAG_nnn'CTG Cuts at: 2126 3933 5505 2126 Size: 1807 1572 2811 Fragments arranged by size: 2811 1807 1572 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 2153 2327 2788 3094 3233 3499 3725 3877 Size: 660 174 461 306 139 266 226 152 Cuts at: 3877 5986 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 660 461 306 266 226 174 152 139 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2879 3515 1870 Size: 73 936 636 4545 Fragments arranged by size: 4545 936 636 73 AvrII C'CTAG_G Cuts at: 3391 3391 Size: 6190 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2853 3511 4966 618 Size: 325 217 627 1066 658 1455 1842 Fragments arranged by size: 1842 1455 1066 658 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 3509 1391 Size: 634 1484 4072 Fragments arranged by size: 4072 1484 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2927 2961 4381 4415 1903 Size: 34 990 34 1420 34 3678 Fragments arranged by size: 3678 1420 990 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3115 4082 5292 3115 Size: 967 1210 4013 Fragments arranged by size: 4013 1210 967 BclI T'GATC_A Cuts at: 1548 3132 1548 Size: 1584 4606 BfrBI ATG'CAT Cuts at: 2030 2030 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2214 3002 3316 6185 2214 Size: 788 314 2869 2219 Fragments arranged by size: 2869 2219 788 314 BlpI GC'TnA_GC Cuts at: 3607 3607 Size: 6190 Bme1580I G_kGCm'C Cuts at: 1790 2845 4251 5407 1790 Size: 1055 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 1055 BmgBI CAC'GTC Cuts at: 1318 3352 1318 Size: 2034 4156 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3251 3921 4879 Size: 416 53 83 399 24 1834 670 958 Cuts at: 4879 442 Size: 1753 Fragments arranged by size: 1834 1753 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 3140 2152 Size: 988 5202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2451 3110 3156 3485 4850 1239 Size: 1212 659 46 329 1365 2579 Fragments arranged by size: 2579 1365 1212 659 329 46 Bpu10I CC'TnA_GC Cuts at: 3470 3470 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 2303 2435 3442 4315 5180 5478 2282 Size: 21 132 1007 873 865 298 2994 Fragments arranged by size: 2994 1007 873 865 298 132 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3170 3246 4853 882 Size: 617 1671 76 1607 2219 Fragments arranged by size: 2219 1671 1607 617 76 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 2462 2694 6184 2462 Size: 232 3490 2468 Fragments arranged by size: 3490 2468 232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3997 4379 5014 275 Size: 53 83 186 936 2464 382 635 1451 Fragments arranged by size: 2464 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 3243 3273 1178 Size: 30 165 30 692 30 1118 30 4095 Fragments arranged by size: 4095 1118 692 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2843 1186 Size: 1657 4533 BseYI C'CCAG_C Cuts at: 2045 5393 5889 2045 Size: 3348 496 2346 Fragments arranged by size: 3348 2346 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5617 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 3142 4251 4336 5407 2025 Size: 1117 1109 85 1071 2808 Fragments arranged by size: 2808 1117 1109 1071 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2845 3142 3509 4251 4336 5407 Size: 399 235 820 297 367 742 85 1071 Cuts at: 5407 1391 Size: 2174 Fragments arranged by size: 2174 1071 820 742 399 367 297 235 85 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2278 3545 844 Size: 1434 1267 3489 Fragments arranged by size: 3489 1434 1267 BsrBI CCG'CTC Cuts at: 1394 1823 2227 4077 1394 Size: 429 404 1850 3507 Fragments arranged by size: 3507 1850 429 404 BsrDI GCAATG_nn' Cuts at: 65 1248 3224 4685 4859 65 Size: 1183 1976 1461 174 1396 Fragments arranged by size: 1976 1461 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4834 5021 1700 Size: 110 3024 187 2869 Fragments arranged by size: 3024 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 2332 4994 2332 Size: 2662 3528 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2214 2305 3002 3316 3520 3887 4272 4289 Size: 242 91 697 314 204 367 385 17 Cuts at: 4289 5741 5827 5839 6185 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 697 385 367 346 314 242 204 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5638 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2952 487 Size: 683 56 1726 3725 Fragments arranged by size: 3725 1726 683 56 BtsI GCAGTG_nn' Cuts at: 2511 3706 4500 4520 2511 Size: 1195 794 20 4181 Fragments arranged by size: 4181 1195 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 2610 3494 2610 Size: 884 5306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3343 3637 4526 Size: 54 1425 50 275 18 1513 294 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1513 1425 889 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2142 2576 3183 4120 1353 Size: 783 6 434 607 937 3423 Fragments arranged by size: 3423 937 783 607 434 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4777 5151 5297 387 Size: 1064 138 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2023 Size: 6190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 2561 2733 2968 3110 3156 3305 Size: 393 819 110 172 235 142 46 149 Cuts at: 3305 3333 3485 4253 4850 5637 5905 1239 Size: 28 152 768 597 787 268 1524 Fragments arranged by size: 1524 819 787 768 597 393 268 235 172 152 149 142 110 46 28 EcoRI G'AATT_C Cuts at: 2153 3499 3877 2153 Size: 1346 378 4466 Fragments arranged by size: 4466 1346 378 EcoRV GAT'ATC Cuts at: 2803 2803 Size: 6190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3042 3495 3042 Size: 453 5737 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2114 2140 2146 2206 Size: 32 135 32 536 6 26 6 60 Cuts at: 2206 2238 3124 3156 4831 4863 4905 4937 1373 Size: 32 886 32 1675 32 42 32 2626 Fragments arranged by size: 2626 1675 886 536 135 60 42 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2496 2923 3534 3780 669 Size: 988 281 558 427 611 246 3079 Fragments arranged by size: 3079 988 611 558 427 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 2857 3515 2857 Size: 658 5532 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2146 5304 5488 1305 Size: 841 3158 184 2007 Fragments arranged by size: 3158 2007 841 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3345 10 Size: 54 1425 343 1513 2855 Fragments arranged by size: 2855 1513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2471 4148 4507 4666 518 Size: 648 1305 1677 359 159 2042 Fragments arranged by size: 2042 1677 1305 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2537 3130 3606 4283 5431 5676 1606 Size: 931 593 476 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 593 476 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5638 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NsiI A_TGCA'T Cuts at: 2032 2032 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 3548 5093 1132 Size: 97 2319 1545 2229 Fragments arranged by size: 2319 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 3352 1108 Size: 2244 3946 PfoI T'CCnGG_A Cuts at: 2719 3462 2719 Size: 743 5447 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspXI vC'TCGA_Gb Cuts at: 2879 2879 Size: 6190 PstI C_TGCA'G Cuts at: 830 2506 2542 2685 3542 830 Size: 1676 36 143 857 3478 Fragments arranged by size: 3478 1676 857 143 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2537 3130 2537 Size: 593 5597 SacI G_AGCT'C Cuts at: 2025 2025 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 3183 3183 Size: 6190 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2502 2538 2565 2639 2681 3538 4673 Size: 219 1457 36 27 74 42 857 1135 Cuts at: 4673 5354 5545 826 Size: 681 191 1471 Fragments arranged by size: 1471 1457 1135 857 681 219 191 74 42 36 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 2318 2414 2879 3421 4294 Size: 197 926 354 21 96 465 542 873 Cuts at: 4294 5195 5457 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 873 542 465 354 262 197 96 21 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 3169 1517 Size: 1652 4538 StyI C'CwwG_G Cuts at: 513 1065 2187 3391 3629 513 Size: 552 1122 1204 238 3074 Fragments arranged by size: 3074 1204 1122 552 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2984 4217 4234 4387 4572 5017 325 Size: 1255 1404 1233 17 153 185 445 1498 Fragments arranged by size: 1498 1404 1255 1233 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4436 6148 96 Size: 274 80 33 51 1443 2459 1712 138 Fragments arranged by size: 2459 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 6150 6182 2095 Size: 32 4023 32 2103 Fragments arranged by size: 4023 2103 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3353 3353 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 2879 1943 Size: 936 5254 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 2328 3318 4319 2328 Size: 990 1001 4199 Fragments arranged by size: 4199 1001 990 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI