POU5F2 ORF clone FHC03892

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Halotag ORF Clone FHC03892

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Clone info.

Species

human

Clone Name

pFN21AB0916 Sequence (with vector) Restriction Map

Product ID

FHC03892

Flexi Clone PID

FXC03892

ORF Sequence

DNA sequence (984 bp)
Protein sequence (328 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527965]

Gene

KIBB0916

Symbol

POU5F2

Alias : SPRM-1

Description

POU domain class 5, transcription factor 2

Original Clone ID

cs02016

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 984 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 328 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS59489.1 4.6e-112 100.0 POU5F2 CCDS34391.1 1.1e-28 47.6 POU5F1 CCDS55274.1 2e-27 47.8 POU5F1B CCDS47398.2 1.4e-21 51.9 POU5F1 CCDS14450.1 4.6e-19 39.3 POU3F4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR013847 137 154 PR00028 POU IPR013847 160 173 PR00028 POU IPR013847 176 191 PR00028 POU IPR013847 209 229 PR00028 POU IPR013847 244 259 PR00028 POU HMMPfam IPR000327 123 192 PF00157 POU-specific IPR001356 223 261 PF00046 Homeobox HMMSmart IPR000327 118 192 SM00352 POU-specific IPR001356 210 272 SM00389 Homeobox ProfileScan IPR000327 118 192 PS51179 POU-specific IPR001356 208 268 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002692 360 164177 47.6 98.8 NP_001153014 359 615739 47.8 94.8 NP_002690 451 602479 34.0 88.4 XP_011541043 403 607394 34.3 80.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB0916 Download

>KIBB0916  984 bp
ATGGCCGGACACAGGCCCTCAAACCACTTCTGCCCCCTTCCAGGCAGTGGTGGGGGCGGC
CCCAGAGGGCCGATGCCCCTGCGGGTTGACACTCTGACCTGGTTGAGCACCCAGGCGGCC
CCTGGCAGGGTGATGGTCTGGCCGGCAGTCAGGCCAGGGATCTGCCCAGGCCCTGACGTG
TGGAGGATTCCCCTGGGTCCCCTGCCACACGAATTCCGGGGCTGGATAGCACCCTGCAGG
CCCCGTCTTGGAGCTAGTGAGGCAGGGGACTGGTTGCGACGCCCCTCCGAAGGCGCCCTC
CCGGGGCCCTACATTGCCCTGCGGAGCATTCCGAAGTTGCCGCCGCCAGAGGACATCTCG
GGCATACTGAAAGAGTTGCAGCAATTGGCCAAGGAGTTGAGGCAGAAGAGGTTGAGCCTA
GGGTACTCGCAGGCCGATGTGGGGATCGCTGTGGGAGCTCTGTTTGGGAAGGTGCTTAGC
CAGACGACCATCTGCCGCTTCGAGGCCCAGCAGCTAAGCGTCGCCAACATGTGGAAGCTG
CGACCACTGCTGAAAAAGTGGCTGAAGGAAGTGGAAGCAGAGAACCTTCTGGGCTTATGC
AAAATGGAGATGATCCTGCAACAGTCTGGGAAGTGGAGACGGGCAAGCAGAGAGCGACGA
ATCGGAAACAGCCTGGAGAAATTCTTCCAGCGGTGCCCTAAGCCCACACCCCAGCAAATC
AGCCACATTGCTGGGTGCCTCCAGCTGCAGAAGGATGTGGTTCGAGTTTGGTTCTATAAC
CGCAGCAAGATGGGCAGTCGACCAACCAATGATGCTTCCCCACGGGAGATTGTGGGGACA
GCCGGGCCTCCTTGCCCAGGAGCACCAGTGTGCTTTCACCTGGGACTGGGGCTCCCAGTG
GATATCCCCCACTATACACGTCTCTACTCTGCAGGGGTAGCCCACTCCTCTGCCCCAGCC
ACCACTCTGGGCCTCCTCAGATTT

ORF Protein

Cloned ORF protein sequence for pFN21AB0916 Download

>KIBB0916  328 aa
MAGHRPSNHFCPLPGSGGGGPRGPMPLRVDTLTWLSTQAAPGRVMVWPAVRPGICPGPDV
WRIPLGPLPHEFRGWIAPCRPRLGASEAGDWLRRPSEGALPGPYIALRSIPKLPPPEDIS
GILKELQQLAKELRQKRLSLGYSQADVGIAVGALFGKVLSQTTICRFEAQQLSVANMWKL
RPLLKKWLKEVEAENLLGLCKMEMILQQSGKWRRASRERRIGNSLEKFFQRCPKPTPQQI
SHIAGCLQLQKDVVRVWFYNRSKMGSRPTNDASPREIVGTAGPPCPGAPVCFHLGLGLPV
DIPHYTRLYSAGVAHSSAPATTLGLLRF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB0916 Download

>pFN21AB0916  5689 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGACACAGGCCCTCAAACCACTTCTGCCC
CCTTCCAGGCAGTGGTGGGGGCGGCCCCAGAGGGCCGATGCCCCTGCGGGTTGACACTCT
GACCTGGTTGAGCACCCAGGCGGCCCCTGGCAGGGTGATGGTCTGGCCGGCAGTCAGGCC
AGGGATCTGCCCAGGCCCTGACGTGTGGAGGATTCCCCTGGGTCCCCTGCCACACGAATT
CCGGGGCTGGATAGCACCCTGCAGGCCCCGTCTTGGAGCTAGTGAGGCAGGGGACTGGTT
GCGACGCCCCTCCGAAGGCGCCCTCCCGGGGCCCTACATTGCCCTGCGGAGCATTCCGAA
GTTGCCGCCGCCAGAGGACATCTCGGGCATACTGAAAGAGTTGCAGCAATTGGCCAAGGA
GTTGAGGCAGAAGAGGTTGAGCCTAGGGTACTCGCAGGCCGATGTGGGGATCGCTGTGGG
AGCTCTGTTTGGGAAGGTGCTTAGCCAGACGACCATCTGCCGCTTCGAGGCCCAGCAGCT
AAGCGTCGCCAACATGTGGAAGCTGCGACCACTGCTGAAAAAGTGGCTGAAGGAAGTGGA
AGCAGAGAACCTTCTGGGCTTATGCAAAATGGAGATGATCCTGCAACAGTCTGGGAAGTG
GAGACGGGCAAGCAGAGAGCGACGAATCGGAAACAGCCTGGAGAAATTCTTCCAGCGGTG
CCCTAAGCCCACACCCCAGCAAATCAGCCACATTGCTGGGTGCCTCCAGCTGCAGAAGGA
TGTGGTTCGAGTTTGGTTCTATAACCGCAGCAAGATGGGCAGTCGACCAACCAATGATGC
TTCCCCACGGGAGATTGTGGGGACAGCCGGGCCTCCTTGCCCAGGAGCACCAGTGTGCTT
TCACCTGGGACTGGGGCTCCCAGTGGATATCCCCCACTATACACGTCTCTACTCTGCAGG
GGTAGCCCACTCCTCTGCCCCAGCCACCACTCTGGGCCTCCTCAGATTTGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT