(Circular) (Six-base) MAPSORT of: pFN21AB0916.seq Check: 2781 from: 1 to: 5689 pFN21AB0916 5689 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2803 3032 1937 Size: 866 229 4594 Fragments arranged by size: 4594 866 229 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 3752 5136 5404 1632 Size: 956 1164 1384 268 1917 Fragments arranged by size: 1917 1384 1164 956 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2181 2532 2921 4588 5565 1203 Size: 978 351 389 1667 977 1327 Fragments arranged by size: 1667 1327 978 977 389 351 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2872 1166 Size: 1706 3983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2890 1184 Size: 1706 3983 AlwNI CAG_nnn'CTG Cuts at: 2177 3432 5004 2177 Size: 1255 1572 2862 Fragments arranged by size: 2862 1572 1255 ApaI G_GGCC'C Cuts at: 2313 2313 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2216 2684 2998 3224 3376 5485 1493 Size: 723 468 314 226 152 2109 1697 Fragments arranged by size: 2109 1697 723 468 314 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2305 2362 3014 1870 Size: 73 362 57 652 4545 Fragments arranged by size: 4545 652 362 73 57 AvrII C'CTAG_G Cuts at: 2422 2422 Size: 5689 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 2697 2739 3010 4465 Size: 325 217 627 510 400 42 271 1455 Cuts at: 4465 618 Size: 1842 Fragments arranged by size: 1842 1455 627 510 400 325 271 217 42 BanII G_rGCy'C Cuts at: 1391 2313 2464 2898 3008 1391 Size: 922 151 434 110 4072 Fragments arranged by size: 4072 922 434 151 110 BbeI G_GCGC'C Cuts at: 2301 2301 Size: 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2065 2099 3880 3914 1903 Size: 34 128 34 1781 34 3678 Fragments arranged by size: 3678 1781 128 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2480 2519 3106 2480 Size: 39 587 5063 Fragments arranged by size: 5063 587 39 Bme1580I G_kGCm'C Cuts at: 1790 2313 2702 3750 4906 1790 Size: 523 389 1048 1156 2573 Fragments arranged by size: 2573 1156 1048 523 389 BmgBI CAC'GTC Cuts at: 1318 2182 2924 1318 Size: 864 742 4083 Fragments arranged by size: 4083 864 742 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2894 2900 3420 Size: 416 53 83 399 24 1477 6 520 Cuts at: 3420 4378 442 Size: 958 1753 Fragments arranged by size: 1753 1477 958 520 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2377 2377 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 2729 4349 1239 Size: 1460 30 1620 2579 Fragments arranged by size: 2579 1620 1460 30 Bpu10I CC'TnA_GC Cuts at: 2703 2703 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2284 2298 3496 3878 Size: 53 83 186 936 751 14 1198 382 Cuts at: 3878 4513 275 Size: 635 1451 Fragments arranged by size: 1451 1198 936 751 635 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2856 2880 2886 2910 1178 Size: 30 165 30 1453 24 6 24 3957 Fragments arranged by size: 3957 1453 165 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2941 2969 1186 Size: 1755 28 3906 Fragments arranged by size: 3906 1755 28 BseYI C'CCAG_C Cuts at: 2511 2715 2735 2959 4892 5388 2511 Size: 204 20 224 1933 496 2812 Fragments arranged by size: 2812 1933 496 224 204 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2114 2464 2869 3750 3835 4906 2114 Size: 350 405 881 85 1071 2897 Fragments arranged by size: 2897 1071 881 405 350 85 BsmI GAATG_Cn' Cuts at: 2331 3200 3293 2331 Size: 869 93 4727 Fragments arranged by size: 4727 869 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2635 2930 2635 Size: 295 5394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2313 2464 2702 2869 2898 3008 Size: 399 324 199 151 238 167 29 110 Cuts at: 3008 3750 3835 4906 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 238 199 167 151 110 85 29 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2316 2730 4184 4358 65 Size: 1183 1068 414 1454 174 1396 Fragments arranged by size: 1454 1396 1183 1068 414 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 4333 4520 1700 Size: 110 336 2187 187 2869 Fragments arranged by size: 2869 2187 336 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2735 2971 2735 Size: 236 5453 BstYI r'GATC_y Cuts at: 1972 2163 3019 3386 3771 3788 5240 5326 5338 Size: 191 856 367 385 17 1452 86 12 Cuts at: 5338 5684 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 856 385 367 346 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2825 513 Size: 552 939 821 3377 Fragments arranged by size: 3377 939 821 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2549 3205 3999 4019 2056 Size: 493 656 794 20 3726 Fragments arranged by size: 3726 794 656 493 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2144 2391 Size: 54 1425 50 275 18 177 137 247 Cuts at: 2391 3136 4025 5654 8 Size: 745 889 1629 43 Fragments arranged by size: 1629 1425 889 745 275 247 177 137 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 3619 1353 Size: 1052 1214 3423 Fragments arranged by size: 3423 1214 1052 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2462 2462 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 2699 2729 3752 4349 5136 5404 Size: 393 956 111 30 1023 597 787 268 Cuts at: 5404 1239 Size: 1524 Fragments arranged by size: 1524 1023 956 787 597 393 268 111 30 EcoNI CCTnn'n_nnAGG Cuts at: 2859 2859 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2019 2174 2201 2244 2309 2310 2019 Size: 155 27 43 65 1 5398 Fragments arranged by size: 5398 155 65 43 27 1 EcoRI G'AATT_C Cuts at: 2216 2998 3376 2216 Size: 782 378 4529 Fragments arranged by size: 4529 782 378 EcoRV GAT'ATC Cuts at: 2908 2908 Size: 5689 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2301 4836 1678 Size: 623 2535 2531 Fragments arranged by size: 2535 2531 623 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2785 2817 2880 2912 4330 Size: 32 135 32 1213 32 63 32 1418 Cuts at: 4330 4362 4404 4436 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1418 1213 135 63 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2804 3033 3279 669 Size: 988 281 154 712 229 246 3079 Fragments arranged by size: 3079 988 712 281 246 229 154 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2297 2297 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 2387 3288 2387 Size: 901 4788 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 10 Size: 54 1425 343 561 3306 Fragments arranged by size: 3306 1425 561 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2872 3647 4006 4165 518 Size: 648 1706 775 359 159 2042 Fragments arranged by size: 2042 1706 775 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2695 2749 3105 3782 4930 5175 1606 Size: 1089 54 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 1089 677 356 245 54 NaeI GCC'GGC Cuts at: 2148 2148 Size: 5689 NarI GG'CG_CC Cuts at: 2298 2298 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2146 2146 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2536 3047 4592 1132 Size: 97 1307 511 1545 2229 Fragments arranged by size: 2229 1545 1307 511 97 PasI CC'CwG_GG Cuts at: 1348 2197 1348 Size: 849 4840 PciI A'CATG_T Cuts at: 2532 4588 2532 Size: 2056 3633 PflMI CCAn_nnn'nTGG Cuts at: 1108 2051 2535 1108 Size: 943 484 4262 Fragments arranged by size: 4262 943 484 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2201 Size: 5689 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2309 2309 Size: 5689 PstI C_TGCA'G Cuts at: 830 2243 2754 2938 3041 830 Size: 1413 511 184 103 3478 Fragments arranged by size: 3478 1413 511 184 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2749 2749 Size: 5689 SacI G_AGCT'C Cuts at: 2464 2464 Size: 5689 SalI G'TCGA_C Cuts at: 1936 2802 3031 1936 Size: 866 229 4594 Fragments arranged by size: 4594 866 229 SanDI GG'GwC_CC Cuts at: 2201 2201 Size: 5689 SbfI CC_TGCA'GG Cuts at: 2243 3041 2243 Size: 798 4891 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SexAI A'CCwGG_T Cuts at: 2102 2102 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2239 2750 2934 3037 4172 4853 5044 Size: 219 1194 511 184 103 1135 681 191 Cuts at: 5044 826 Size: 1471 Fragments arranged by size: 1471 1194 1135 681 511 219 191 184 103 SfoI GGC'GCC Cuts at: 2299 2299 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2307 3016 1872 Size: 435 709 4545 Fragments arranged by size: 4545 709 435 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2004 2394 2422 3128 513 Size: 552 939 390 28 706 3074 Fragments arranged by size: 3074 939 706 552 390 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2727 3716 3733 3886 4071 4516 Size: 1255 549 598 989 17 153 185 445 Cuts at: 4516 325 Size: 1498 Fragments arranged by size: 1498 1255 989 598 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2202 2234 2856 2888 5649 5681 2202 Size: 32 622 32 2761 32 2210 Fragments arranged by size: 2761 2210 622 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2123 2123 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2305 3014 1870 Size: 435 709 4545 Fragments arranged by size: 4545 709 435 XmnI GAAnn'nnTTC Cuts at: 2687 3818 2687 Size: 1131 4558 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI