DEF6 ORF clone FHC03465

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03465

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Clone info.

Species

human

Clone Name

pFN21AB6134 Sequence (with vector) Restriction Map

Product ID

FHC03465

Flexi Clone PID

FXC03465

ORF Sequence

DNA sequence (1893 bp)
Protein sequence (631 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527549]

Gene

KIBB6134

Symbol

DEF6

Alias : IBP, SLAT, SWAP70L

Description

DEF6 guanine nucleotide exchange factor

Original Clone ID

cs00734

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1893 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 631 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4802.1 6.4e-88 99.8 DEF6 CCDS31426.1 2.3e-33 45.6 SWAP70 CCDS73257.1 1.8e-19 40.6 SWAP70 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 218 311 PF00169 Pleckstrin homology domain HMMSmart IPR001849 217 314 SM00233 Pleckstrin homology domain ProfileScan IPR001849 216 312 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_071330 631 610094 99.8 100.0 NP_055870 585 604762 45.6 92.2 NP_001284643 527 604762 40.6 92.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6134 Download

>KIBB6134  1893 bp
ATGGCCCTGCGCAAGGAACTGCTCAAGTCCATCTGGTACGCCTTTACCGCGCTGGACGTG
GAGAAGAGTGGCAAAGTCTCCAAGTCCCAGCTCAAGGTGCTGTCCCACAACCTGTACACG
GTCCTGCACATCCCCCATGACCCCGTGGCCCTGGAGGAACACTTCCGAGATGATGATGAC
GGCCCTGTGTCCAGCCAGGGATACATGCCCTACCTCAACAAGTACATCCTGGACAAGGTG
GAGGAGGGGGCTTTTGTTAAAGAGCACTTTGATGAGCTGTGCTGGACGCTGACGGCCAAG
AAGAACTATCGGGCAGATAGCAACGGGAACAGTATGCTCTCCAATCAGGATGCCTTCCGC
CTCTGGTGCCTCTTCAACTTCCTGTCTGAGGACAAGTACCCTCTGATCATGGTTCCTGAT
GAGGTGGAATACCTGCTGAAAAAGGTACTCAGCAGCATGAGCTTGGAGGTGAGCTTGGGT
GAGCTGGAGGAGCTTCTGGCCCAGGAGGCCCAGGTGGCCCAGACCACCGGGGGGCTCAGC
GTCTGGCAGTTCCTGGAGCTCTTCAATTCGGGCCGCTGCCTGCGGGGCGTGGGCCGGGAC
ACCCTCAGCATGGCCATCCACGAGGTCTACCAGGAGCTCATCCAAGATGTCCTGAAGCAG
GGCTACCTGTGGAAGCGAGGGCACCTGAGAAGGAACTGGGCCGAACGCTGGTTCCAGCTG
CAGCCCAGCTGCCTCTGCTACTTTGGGAGTGAAGAGTGCAAAGAGAAAAGGGGCATTATC
CCGCTGGATGCACACTGCTGCGTGGAGGTGCTGCCAGACCGCGACGGAAAGCGCTGCATG
TTCTGTGTGAAGACAGCCACCCGCACGTATGAGATGAGCGCCTCAGACACGCGCCAGCGC
CAGGAGTGGACAGCTGCCATCCAGATGGCGATCCGGCTGCAGGCCGAGGGGAAGACGTCC
CTACACAAGGACCTGAAGCAGAAACGGCGCGAGCAGCGGGAGCAGCGGGAGCGGCGCCGG
GCGGCCAAGGAAGAGGAGCTGCTGCGGCTGCAGCAGCTGCAGGAGGAGAAGGAGCGGAAG
CTGCAGGAGCTGGAGCTGCTGCAGGAGGCGCAGCGGCAGGCCGAGCGGCTGCTGCAGGAG
GAGGAGGAACGGCGCCGCAGCCAGCACCGCGAGCTGCAGCAGGCGCTCGAGGGCCAACTG
CGCGAGGCGGAGCAGGCCCGGGCCTCCATGCAGGCTGAGATGGAGCTGAAGGAGGAGGAG
GCTGCCCGGCAGCGGCAGCGCATCAAGGAGCTGGAGGAGATGCAGCAGCGGTTGCAGGAG
GCCCTGCAACTAGAGGTGAAAGCTCGGCGAGATGAAGAATCTGTGCGAATCGCTCAGACC
AGACTGCTGGAAGAGGAGGAAGAGAAGCTGAAGCAGTTGATGCAGCTGAAGGAGGAGCAG
GAGCGCTACATCGAACGGGCGCAGCAGGAGAAGGAAGAGCTGCAGCAGGAGATGGCACAG
CAGAGCCGCTCCCTGCAGCAGGCCCAGCAGCAGCTGGAGGAGGTGCGGCAGAACCGGCAG
AGGGCTGACGAGGATGTGGAGGCTGCCCAGAGAAAACTGCGCCAGGCCAGCACCAACGTG
AAACACTGGAATGTCCAGATGAACCGGCTGATGCATCCAATTGAGCCTGGAGATAAGCGT
CCGGTCACCAGCAGCTCCTTCTCAGGCTTCCAGCCCCCTCTGCTTGCCCACCGTGACTCC
TCCCTAAAGCGCCTGACCCGCTGGGGATCCCAGGGCAACAGGACCCCCTCGCCCAACAGC
AATGAGCAGCAGAAGTCCCTCAATGGTGGGGATGAGGCTCCTGCCCCGGCTTCCACCCCT
CAGGAAGATAAACTGGATCCAGCACCAGAAAAT

ORF Protein

Cloned ORF protein sequence for pFN21AB6134 Download

>KIBB6134  631 aa
MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLSHNLYTVLHIPHDPVALEEHFRDDDD
GPVSSQGYMPYLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRADSNGNSMLSNQDAFR
LWCLFNFLSEDKYPLIMVPDEVEYLLKKVLSSMSLEVSLGELEELLAQEAQVAQTTGGLS
VWQFLELFNSGRCLRGVGRDTLSMAIHEVYQELIQDVLKQGYLWKRGHLRRNWAERWFQL
QPSCLCYFGSEECKEKRGIIPLDAHCCVEVLPDRDGKRCMFCVKTATRTYEMSASDTRQR
QEWTAAIQMAIRLQAEGKTSLHKDLKQKRREQREQRERRRAAKEEELLRLQQLQEEKERK
LQELELLQEAQRQAERLLQEEEERRRSQHRELQQALEGQLREAEQARASMQAEMELKEEE
AARQRQRIKELEEMQQRLQEALQLEVKARRDEESVRIAQTRLLEEEEEKLKQLMQLKEEQ
ERYIERAQQEKEELQQEMAQQSRSLQQAQQQLEEVRQNRQRADEDVEAAQRKLRQASTNV
KHWNVQMNRLMHPIEPGDKRPVTSSSFSGFQPPLLAHRDSSLKRLTRWGSQGNRTPSPNS
NEQQKSLNGGDEAPAPASTPQEDKLDPAPEN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6134 Download

>pFN21AB6134  6598 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCTGCGCAAGGAACTGCTCAAGTCCATCTG
GTACGCCTTTACCGCGCTGGACGTGGAGAAGAGTGGCAAAGTCTCCAAGTCCCAGCTCAA
GGTGCTGTCCCACAACCTGTACACGGTCCTGCACATCCCCCATGACCCCGTGGCCCTGGA
GGAACACTTCCGAGATGATGATGACGGCCCTGTGTCCAGCCAGGGATACATGCCCTACCT
CAACAAGTACATCCTGGACAAGGTGGAGGAGGGGGCTTTTGTTAAAGAGCACTTTGATGA
GCTGTGCTGGACGCTGACGGCCAAGAAGAACTATCGGGCAGATAGCAACGGGAACAGTAT
GCTCTCCAATCAGGATGCCTTCCGCCTCTGGTGCCTCTTCAACTTCCTGTCTGAGGACAA
GTACCCTCTGATCATGGTTCCTGATGAGGTGGAATACCTGCTGAAAAAGGTACTCAGCAG
CATGAGCTTGGAGGTGAGCTTGGGTGAGCTGGAGGAGCTTCTGGCCCAGGAGGCCCAGGT
GGCCCAGACCACCGGGGGGCTCAGCGTCTGGCAGTTCCTGGAGCTCTTCAATTCGGGCCG
CTGCCTGCGGGGCGTGGGCCGGGACACCCTCAGCATGGCCATCCACGAGGTCTACCAGGA
GCTCATCCAAGATGTCCTGAAGCAGGGCTACCTGTGGAAGCGAGGGCACCTGAGAAGGAA
CTGGGCCGAACGCTGGTTCCAGCTGCAGCCCAGCTGCCTCTGCTACTTTGGGAGTGAAGA
GTGCAAAGAGAAAAGGGGCATTATCCCGCTGGATGCACACTGCTGCGTGGAGGTGCTGCC
AGACCGCGACGGAAAGCGCTGCATGTTCTGTGTGAAGACAGCCACCCGCACGTATGAGAT
GAGCGCCTCAGACACGCGCCAGCGCCAGGAGTGGACAGCTGCCATCCAGATGGCGATCCG
GCTGCAGGCCGAGGGGAAGACGTCCCTACACAAGGACCTGAAGCAGAAACGGCGCGAGCA
GCGGGAGCAGCGGGAGCGGCGCCGGGCGGCCAAGGAAGAGGAGCTGCTGCGGCTGCAGCA
GCTGCAGGAGGAGAAGGAGCGGAAGCTGCAGGAGCTGGAGCTGCTGCAGGAGGCGCAGCG
GCAGGCCGAGCGGCTGCTGCAGGAGGAGGAGGAACGGCGCCGCAGCCAGCACCGCGAGCT
GCAGCAGGCGCTCGAGGGCCAACTGCGCGAGGCGGAGCAGGCCCGGGCCTCCATGCAGGC
TGAGATGGAGCTGAAGGAGGAGGAGGCTGCCCGGCAGCGGCAGCGCATCAAGGAGCTGGA
GGAGATGCAGCAGCGGTTGCAGGAGGCCCTGCAACTAGAGGTGAAAGCTCGGCGAGATGA
AGAATCTGTGCGAATCGCTCAGACCAGACTGCTGGAAGAGGAGGAAGAGAAGCTGAAGCA
GTTGATGCAGCTGAAGGAGGAGCAGGAGCGCTACATCGAACGGGCGCAGCAGGAGAAGGA
AGAGCTGCAGCAGGAGATGGCACAGCAGAGCCGCTCCCTGCAGCAGGCCCAGCAGCAGCT
GGAGGAGGTGCGGCAGAACCGGCAGAGGGCTGACGAGGATGTGGAGGCTGCCCAGAGAAA
ACTGCGCCAGGCCAGCACCAACGTGAAACACTGGAATGTCCAGATGAACCGGCTGATGCA
TCCAATTGAGCCTGGAGATAAGCGTCCGGTCACCAGCAGCTCCTTCTCAGGCTTCCAGCC
CCCTCTGCTTGCCCACCGTGACTCCTCCCTAAAGCGCCTGACCCGCTGGGGATCCCAGGG
CAACAGGACCCCCTCGCCCAACAGCAATGAGCAGCAGAAGTCCCTCAATGGTGGGGATGA
GGCTCCTGCCCCGGCTTCCACCCCTCAGGAAGATAAACTGGATCCAGCACCAGAAAATGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT