(Circular) (Six-base) MAPSORT of: pFN21AB6134.seq Check: 8450 from: 1 to: 6598 pFN21AB6134 6598 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2963 4408 278 Size: 53 83 186 936 1427 1445 2468 Fragments arranged by size: 2468 1445 1427 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3941 1937 Size: 694 1310 4594 Fragments arranged by size: 4594 1310 694 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 2999 3272 3434 3452 4661 6045 6313 Size: 1046 321 273 162 18 1209 1384 268 Cuts at: 6313 1632 Size: 1917 Fragments arranged by size: 1917 1384 1209 1046 321 273 268 162 18 AfeI AGC'GCT Cuts at: 2837 3449 2837 Size: 612 5986 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 5497 6474 1203 Size: 4294 977 1327 Fragments arranged by size: 4294 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 3620 1166 Size: 2454 4144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2039 2818 1184 Size: 855 779 4964 Fragments arranged by size: 4964 855 779 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 2700 Size: 32 6566 AlwNI CAG_nnn'CTG Cuts at: 2547 2557 3061 3094 3538 4341 5913 2547 Size: 10 504 33 444 803 1572 3232 Fragments arranged by size: 3232 1572 803 504 444 33 10 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 3907 4133 4285 6394 1493 Size: 2414 226 152 2109 1697 Fragments arranged by size: 2414 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 3191 3222 3923 1870 Size: 73 1248 31 701 4545 Fragments arranged by size: 4545 1248 701 73 31 BamHI G'GATC_C Cuts at: 3770 3880 3928 4295 3770 Size: 110 48 367 6073 Fragments arranged by size: 6073 367 110 48 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2370 2685 3018 3156 3919 Size: 325 217 627 583 315 333 138 763 Cuts at: 3919 5374 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 763 627 583 333 325 315 217 138 BanII G_rGCy'C Cuts at: 1391 2541 2565 2643 3917 1391 Size: 1150 24 78 1274 4072 Fragments arranged by size: 4072 1274 1150 78 24 BbeI G_GCGC'C Cuts at: 3022 3160 3022 Size: 138 6460 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 2963 928 Size: 971 962 102 4563 Fragments arranged by size: 4563 971 962 102 BbvCI CC'TCA_GC Cuts at: 2609 2609 Size: 6598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2923 2957 4789 4823 1903 Size: 34 986 34 1832 34 3678 Fragments arranged by size: 3678 1832 986 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2198 4490 5700 2198 Size: 2292 1210 3096 Fragments arranged by size: 3096 2292 1210 BclI T'GATC_A Cuts at: 1548 2409 1548 Size: 861 5737 BfrBI ATG'CAT Cuts at: 3658 3658 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2510 2519 2589 Size: 107 122 71 631 605 838 9 70 Cuts at: 2589 5209 136 Size: 2620 1525 Fragments arranged by size: 2620 1525 838 631 605 122 107 71 70 9 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 2540 4015 2540 Size: 1475 5123 Bme1580I G_kGCm'C Cuts at: 1790 2688 4659 5815 1790 Size: 898 1971 1156 2573 Fragments arranged by size: 2573 1971 1156 898 BmgBI CAC'GTC Cuts at: 1318 2062 1318 Size: 744 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 4329 5287 Size: 416 53 83 399 24 1293 1619 958 Cuts at: 5287 442 Size: 1753 Fragments arranged by size: 1753 1619 1293 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2510 2579 3116 3317 3560 3693 5258 Size: 938 333 69 537 201 243 133 1565 Cuts at: 5258 1239 Size: 2579 Fragments arranged by size: 2579 1565 938 537 333 243 201 133 69 Bpu10I CC'TnA_GC Cuts at: 2609 2609 Size: 6598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2012 2081 4723 5588 5886 2012 Size: 69 2642 865 298 2724 Fragments arranged by size: 2724 2642 865 298 69 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5261 882 Size: 617 3762 2219 Fragments arranged by size: 3762 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2871 4389 493 Size: 711 1667 1518 2702 Fragments arranged by size: 2702 1667 1518 711 BsaBI GATnn'nnATC Cuts at: 2933 6592 2933 Size: 3659 2939 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2960 3019 3157 4405 Size: 53 83 186 936 1427 59 138 1248 Cuts at: 4405 4787 5422 275 Size: 382 635 1451 Fragments arranged by size: 1451 1427 1248 936 635 382 186 138 83 59 53 BsaWI w'CCGG_w Cuts at: 3685 5031 5429 5703 5850 6261 3685 Size: 1346 398 274 147 411 4022 Fragments arranged by size: 4022 1346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3725 3755 1178 Size: 30 165 30 2322 30 4021 Fragments arranged by size: 4021 2322 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2507 3053 3083 3158 3161 3272 3275 Size: 1075 246 546 30 75 3 111 3 Cuts at: 3275 3314 3413 3452 3557 3733 1186 Size: 39 99 39 105 176 4051 Fragments arranged by size: 4051 1075 546 246 176 111 105 99 75 39 39 30 3 3 BseYI C'CCAG_C Cuts at: 2091 2729 3528 3765 5801 6297 2091 Size: 638 799 237 2036 496 2392 Fragments arranged by size: 2392 2036 799 638 496 237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 1163 Size: 573 378 5647 Fragments arranged by size: 5647 573 378 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4809 5837 1540 Size: 275 186 2047 761 1028 2301 Fragments arranged by size: 2301 2047 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 2565 2643 4659 4744 5815 2271 Size: 294 78 2016 85 1071 3054 Fragments arranged by size: 3054 2016 1071 294 85 78 BsmI GAATG_Cn' Cuts at: 4109 4202 4109 Size: 93 6505 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2541 2565 2643 2688 3917 4659 Size: 399 481 270 24 78 45 1229 742 Cuts at: 4659 4744 5815 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1229 1071 742 481 399 270 85 78 45 24 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 4487 6217 4487 Size: 1730 4868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2445 3953 844 Size: 1601 1508 3489 Fragments arranged by size: 3489 1601 1508 BsrBI CCG'CTC Cuts at: 1394 1823 3016 3079 3130 3513 4485 1394 Size: 429 1193 63 51 383 972 3507 Fragments arranged by size: 3507 1193 972 429 383 63 51 BsrDI GCAATG_nn' Cuts at: 65 1248 3811 5093 5267 65 Size: 1183 2563 1282 174 1396 Fragments arranged by size: 2563 1396 1282 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3558 3648 5242 5429 1700 Size: 110 1748 90 1594 187 2869 Fragments arranged by size: 2869 1748 1594 187 110 90 BsrGI T'GTAC_A Cuts at: 96 2118 96 Size: 2022 4576 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 2624 4658 5670 2624 Size: 2034 1012 3552 Fragments arranged by size: 3552 2034 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3061 3316 3424 794 Size: 2267 255 108 3968 Fragments arranged by size: 3968 2267 255 108 BstBI TT'CG_AA Cuts at: 5402 5402 Size: 6598 BstEII G'GTnAC_C Cuts at: 1184 3688 1184 Size: 2504 4094 BstXI CCAn_nnnn'nTGG Cuts at: 2929 3391 2929 Size: 462 6136 BstYI r'GATC_y Cuts at: 1972 3770 3880 3928 4295 4680 4697 6149 6235 Size: 1798 110 48 367 385 17 1452 86 Cuts at: 6235 6247 6593 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1798 1452 385 367 346 110 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3864 3864 Size: 6598 BtgI C'CryG_G Cuts at: 513 1065 2004 2148 513 Size: 552 939 144 4963 Fragments arranged by size: 4963 939 552 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5859 Fragments arranged by size: 5859 683 56 BtsI GCAGTG_nn' Cuts at: 2797 4114 4908 4928 2797 Size: 1317 794 20 4467 Fragments arranged by size: 4467 1317 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2616 3027 Size: 54 1425 50 275 18 468 318 411 Cuts at: 3027 4045 4934 6563 8 Size: 1018 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 468 411 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2381 2570 2751 3030 3390 3399 3474 Size: 710 318 189 181 279 360 9 75 Cuts at: 3474 4528 1353 Size: 1054 3423 Fragments arranged by size: 3423 1054 710 360 318 279 189 181 75 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2351 3227 5185 5559 5705 387 Size: 1064 138 762 876 1958 374 146 1280 Fragments arranged by size: 1958 1280 1064 876 762 374 146 138 EcoICRI GAG'CTC Cuts at: 2563 2641 2563 Size: 78 6520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2510 2579 2678 2999 3116 3272 Size: 393 545 333 69 99 321 117 156 Cuts at: 3272 3317 3434 3452 3560 3693 4661 5258 6045 Size: 45 117 18 108 133 968 597 787 Cuts at: 6045 6313 1239 Size: 268 1524 Fragments arranged by size: 1524 968 787 597 545 393 333 321 268 156 133 117 117 108 99 69 45 18 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2237 2390 2660 2969 3521 2015 Size: 222 153 270 309 552 5092 Fragments arranged by size: 5092 552 309 270 222 153 EcoO109I rG'GnC_Cy Cuts at: 2974 3325 3786 2974 Size: 351 461 5786 Fragments arranged by size: 5786 461 351 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 FspI TGC'GCA Cuts at: 2015 5104 2015 Size: 3089 3509 HaeII r_GCGC'y Cuts at: 1678 2839 2886 2905 3022 3160 3191 3451 3757 Size: 1161 47 19 117 138 31 260 306 Cuts at: 3757 5745 1678 Size: 1988 2531 Fragments arranged by size: 2531 1988 1161 306 260 138 117 47 31 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3347 3379 5239 5271 5313 Size: 32 135 32 1775 32 1860 32 42 Cuts at: 5313 5345 1373 Size: 32 2626 Fragments arranged by size: 2626 1860 1775 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3942 4188 669 Size: 988 281 2004 246 3079 Fragments arranged by size: 3079 2004 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KasI G'GCGC_C Cuts at: 3018 3156 3018 Size: 138 6460 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 3663 4197 3663 Size: 534 6064 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5712 5896 1305 Size: 4407 184 2007 Fragments arranged by size: 4407 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 10 Size: 54 1425 343 786 3990 Fragments arranged by size: 3990 1425 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2927 3620 3633 4556 4915 5074 Size: 648 1108 653 693 13 923 359 159 Cuts at: 5074 518 Size: 2042 Fragments arranged by size: 2042 1108 923 693 653 648 359 159 13 MspA1I CmG'CkG Cuts at: 1606 2580 2722 2733 2788 2918 3001 3010 3061 Size: 974 142 11 55 130 83 9 51 Cuts at: 3061 3118 3277 3313 3430 3538 3765 4014 4691 Size: 57 159 36 117 108 227 249 677 Cuts at: 4691 5839 6084 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 974 677 249 245 227 159 142 130 117 108 83 57 55 51 36 11 9 NarI GG'CG_CC Cuts at: 3019 3157 3019 Size: 138 6460 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5107 Fragments arranged by size: 5107 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NsiI A_TGCA'T Cuts at: 3660 3660 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2212 2845 3956 5501 1132 Size: 97 983 633 1111 1545 2229 Fragments arranged by size: 2229 1545 1111 983 633 97 PasI CC'CwG_GG Cuts at: 1348 3775 1348 Size: 2427 4171 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PfoI T'CCnGG_A Cuts at: 2232 2556 2232 Size: 324 6274 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2974 3786 2974 Size: 812 5786 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspXI vC'TCGA_Gb Cuts at: 3191 3191 Size: 6598 PstI C_TGCA'G Cuts at: 830 2727 2946 3057 3066 3090 3108 3141 3183 Size: 1897 219 111 9 24 18 33 42 Cuts at: 3183 3489 3522 3950 830 Size: 306 33 428 3478 Fragments arranged by size: 3478 1897 428 306 219 111 42 33 33 24 18 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2722 2733 2918 3061 3430 3538 2722 Size: 11 185 143 369 108 5782 Fragments arranged by size: 5782 369 185 143 108 11 SacI G_AGCT'C Cuts at: 2565 2643 2565 Size: 78 6520 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2570 3474 2570 Size: 904 5694 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SfcI C'TryA_G Cuts at: 826 1045 2723 2942 3053 3062 3086 3104 3137 Size: 219 1678 219 111 9 24 18 33 Cuts at: 3137 3179 3485 3518 3946 5081 5762 5953 826 Size: 42 306 33 428 1135 681 191 1471 Fragments arranged by size: 1678 1471 1135 681 428 306 219 219 191 111 42 33 33 24 18 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2510 2519 2510 Size: 9 6589 SfoI GGC'GCC Cuts at: 3020 3158 3020 Size: 138 6460 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3224 3925 1872 Size: 1352 701 4545 Fragments arranged by size: 4545 1352 701 SmlI C'TyrA_G Cuts at: 820 1017 1943 2027 2096 3191 4702 5603 5865 Size: 197 926 84 69 1095 1511 901 262 Cuts at: 5865 820 Size: 1553 Fragments arranged by size: 1553 1511 1095 926 901 262 197 84 69 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SrfI GCCC'GGGC Cuts at: 3224 3224 Size: 6598 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 2004 3030 4037 513 Size: 552 939 1026 1007 3074 Fragments arranged by size: 3074 1026 1007 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2471 4625 4642 4795 4980 5425 325 Size: 1255 891 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 891 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2226 4844 Size: 274 80 33 51 1443 141 108 2618 Cuts at: 4844 6556 96 Size: 1712 138 Fragments arranged by size: 2618 1712 1443 274 141 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3725 3757 6558 6590 3725 Size: 32 2801 32 3733 Fragments arranged by size: 3733 2801 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6598 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 3191 1943 Size: 1248 5350 XmaI C'CCGG_G Cuts at: 1870 3222 3923 1870 Size: 1352 701 4545 Fragments arranged by size: 4545 1352 701 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2961 4406 276 Size: 53 83 186 936 1427 1445 2468 Fragments arranged by size: 2468 1445 1427 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SwaI XcmI