RGS7 ORF clone FHC03429

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03429

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Clone info.

Species

human

Clone Name

pFN21AB5852 Sequence (with vector) Restriction Map

Product ID

FHC03429

Flexi Clone PID

FXC03429

ORF Sequence

DNA sequence (1407 bp)
Protein sequence (469 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527513]

Gene

KIBB5852

Symbol

RGS7

Description

regulator of G-protein signaling 7, transcript variant 4

Original Clone ID

cs00552

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1407 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 469 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS60458.1 3.1e-175 100.0 RGS7 CCDS60459.1 9.1e-171 99.3 RGS7 CCDS31071.1 2.6e-170 96.3 RGS7 CCDS9808.1 6e-133 73.5 RGS6 CCDS55924.1 4e-130 70.8 RGS6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000342 330 351 PR01301 Regulator of G protein signalling IPR000342 352 370 PR01301 Regulator of G protein signalling IPR000342 380 403 PR01301 Regulator of G protein signalling IPR000342 422 441 PR01301 Regulator of G protein signalling HMMPfam IPR000591 40 110 PF00610 DEP domain IPR015898 256 307 PF00631 G-protein gamma domain IPR000342 335 447 PF00615 Regulator of G protein signalling HMMSmart IPR000591 37 112 SM00049 DEP domain IPR015898 255 316 SM00224 G-protein gamma domain IPR000342 333 448 SM00315 Regulator of G protein signalling ProfileScan IPR000591 37 112 PS50186 DEP domain IPR000342 333 448 PS50132 Regulator of G protein signalling
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001269707 469 602517 100.0 100.0 NP_001269704 477 602517 99.3 97.4 NP_002915 487 602517 96.3 100.0 XP_005273275 495 602517 98.1 98.7 XP_016857497 443 602517 100.0 94.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5852 Download

>KIBB5852  1407 bp
ATGGCCCAGGGGAATAATTATGGGCAGACCAGCAACGGGGTGGCCGATGAATCACCCAAC
ATGCTGGTGTACAGAAAGATGGAAGACGTCATAGCACGGATGCAAGATGAAAAAAATGGA
ATTCCTATTCGTACGGTCAAAAGCTTTCTTTCCAAGATACCTAGCGTCTTCTCTGGTTCA
GACATTGTTCAATGGTTGATAAAGAACTTAACTATAGAAGATCCAGTGGAGGCGCTCCAT
TTGGGAACATTAATGGCTGCCCACGGCTACTTCTTTCCAATCTCAGATCATGTCCTCACA
CTCAAGGATGATGGCACCTTTTACCGGTTTCAAACCCCCTATTTTTGGCCATCAAATTGT
TGGGAGCCGGAAAACACAGATTATGCCGTTTACCTCTGCAAGAGAACAATGCAAAACAAG
GCACGACTGGAGCTCGCAGACTATGAGGCTGAGAGCCTGGCCAGGCTGCAGAGAGCATTT
GCCCGGAAGTGGGAGTTCATTTTCATGCAAGCAGAAGCACAAGCAAAAGTGGACAAGAAG
AGAGACAAGATTGAAAGGAAGATCCTTGACAGCCAAGAGAGAGCGTTCTGGGACGTGCAC
AGGCCCGTGCCTGGATGTGTAAATACAACTGAAGTGGACATTAAGAAGTCATCCAGAATG
AGAAACCCCCACAAAACACGGAAGTCTGTCTATGGTTTACAAAATGATATTAGAAGTCAC
AGTCCTACCCACACACCCACACCAGAAACTAAACCTCCAACAGAAGATGAGTTACAACAA
CAGATAAAATATTGGCAAATACAGTTAGATAGACATCGGTTAAAAATGTCAAAAGTCGCT
GACAGTCTACTAAGTTACACGGAACAGTATTTAGAATACGACCCGTTTCTTTTGCCACCT
GACCCTTCTAACCCATGGCTGTCCGATGACACCACTTTCTGGGAACTTGAGGCAAGCAAA
GAACCGAGCCAGCAGAGGGTAAAACGATGGGGTTTTGGCATGGACGAGGCATTGAAAGAC
CCAGTTGGGAGAGAACAGTTCCTTAAATTTCTAGAGTCAGAATTCAGCTCGGAAAATTTA
AGATTCTGGCTGGCAGTGGAGGACCTGAAAAAGAGGCCTATTAAAGAAGTACCCTCAAGA
GTTCAGGAAATATGGCAAGAGTTTCTGGCTCCCGGAGCCCCCAGTGCTATTAACTTGGAT
TCCAAGAGTTATGACAAAACCACACAGAACGTGAAGGAACCTGGACGATACACATTTGAA
GATGCTCAGGAGCACATTTACAAACTGATGAAAAGTGATTCATACCCACGTTTTATAAGA
TCCAGTGCCTATCAGGAGCTTCTACAGGCAAAGAAAAAGGGGAAATCTCTCACGTCCAAG
AGGTTAACAAGCCTTGCTCAGTCTTAC

ORF Protein

Cloned ORF protein sequence for pFN21AB5852 Download

>KIBB5852  469 aa
MAQGNNYGQTSNGVADESPNMLVYRKMEDVIARMQDEKNGIPIRTVKSFLSKIPSVFSGS
DIVQWLIKNLTIEDPVEALHLGTLMAAHGYFFPISDHVLTLKDDGTFYRFQTPYFWPSNC
WEPENTDYAVYLCKRTMQNKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKK
RDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSH
SPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPP
DPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENL
RFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTQNVKEPGRYTFE
DAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKGKSLTSKRLTSLAQSY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5852 Download

>pFN21AB5852  6112 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCAGGGGAATAATTATGGGCAGACCAGCAA
CGGGGTGGCCGATGAATCACCCAACATGCTGGTGTACAGAAAGATGGAAGACGTCATAGC
ACGGATGCAAGATGAAAAAAATGGAATTCCTATTCGTACGGTCAAAAGCTTTCTTTCCAA
GATACCTAGCGTCTTCTCTGGTTCAGACATTGTTCAATGGTTGATAAAGAACTTAACTAT
AGAAGATCCAGTGGAGGCGCTCCATTTGGGAACATTAATGGCTGCCCACGGCTACTTCTT
TCCAATCTCAGATCATGTCCTCACACTCAAGGATGATGGCACCTTTTACCGGTTTCAAAC
CCCCTATTTTTGGCCATCAAATTGTTGGGAGCCGGAAAACACAGATTATGCCGTTTACCT
CTGCAAGAGAACAATGCAAAACAAGGCACGACTGGAGCTCGCAGACTATGAGGCTGAGAG
CCTGGCCAGGCTGCAGAGAGCATTTGCCCGGAAGTGGGAGTTCATTTTCATGCAAGCAGA
AGCACAAGCAAAAGTGGACAAGAAGAGAGACAAGATTGAAAGGAAGATCCTTGACAGCCA
AGAGAGAGCGTTCTGGGACGTGCACAGGCCCGTGCCTGGATGTGTAAATACAACTGAAGT
GGACATTAAGAAGTCATCCAGAATGAGAAACCCCCACAAAACACGGAAGTCTGTCTATGG
TTTACAAAATGATATTAGAAGTCACAGTCCTACCCACACACCCACACCAGAAACTAAACC
TCCAACAGAAGATGAGTTACAACAACAGATAAAATATTGGCAAATACAGTTAGATAGACA
TCGGTTAAAAATGTCAAAAGTCGCTGACAGTCTACTAAGTTACACGGAACAGTATTTAGA
ATACGACCCGTTTCTTTTGCCACCTGACCCTTCTAACCCATGGCTGTCCGATGACACCAC
TTTCTGGGAACTTGAGGCAAGCAAAGAACCGAGCCAGCAGAGGGTAAAACGATGGGGTTT
TGGCATGGACGAGGCATTGAAAGACCCAGTTGGGAGAGAACAGTTCCTTAAATTTCTAGA
GTCAGAATTCAGCTCGGAAAATTTAAGATTCTGGCTGGCAGTGGAGGACCTGAAAAAGAG
GCCTATTAAAGAAGTACCCTCAAGAGTTCAGGAAATATGGCAAGAGTTTCTGGCTCCCGG
AGCCCCCAGTGCTATTAACTTGGATTCCAAGAGTTATGACAAAACCACACAGAACGTGAA
GGAACCTGGACGATACACATTTGAAGATGCTCAGGAGCACATTTACAAACTGATGAAAAG
TGATTCATACCCACGTTTTATAAGATCCAGTGCCTATCAGGAGCTTCTACAGGCAAAGAA
AAAGGGGAAATCTCTCACGTCCAAGAGGTTAACAAGCCTTGCTCAGTCTTACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT