(Circular) (Six-base) MAPSORT of: pFN21AB5852.seq Check: 7517 from: 1 to: 6112 pFN21AB5852 6112 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2094 3922 278 Size: 53 83 186 936 558 1828 2468 Fragments arranged by size: 2468 1828 936 558 186 83 53 AccI GT'mk_AC Cuts at: 1937 2851 3455 1937 Size: 914 604 4594 Fragments arranged by size: 4594 914 604 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2655 4175 5559 5827 1632 Size: 1023 1520 1384 268 1917 Fragments arranged by size: 1917 1520 1384 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 2328 4943 2328 Size: 2615 3497 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 3855 5427 3855 Size: 1572 4540 ApaLI G'TGCA_C Cuts at: 2600 4169 5325 2600 Size: 1569 1156 3387 Fragments arranged by size: 3387 1569 1156 ApoI r'AATT_y Cuts at: 1493 2124 3050 3065 3079 3421 3647 3799 5908 Size: 631 926 15 14 342 226 152 2109 Cuts at: 5908 1493 Size: 1697 Fragments arranged by size: 2109 1697 926 631 342 226 152 15 14 AseI AT'TA_AT Cuts at: 160 1032 2255 4666 160 Size: 872 1223 2411 1606 Fragments arranged by size: 2411 1606 1223 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 3437 1870 Size: 73 1494 4545 Fragments arranged by size: 4545 1494 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2318 3433 4888 618 Size: 325 217 627 531 1115 1455 1842 Fragments arranged by size: 1842 1455 1115 627 531 325 217 BanII G_rGCy'C Cuts at: 1391 2439 3184 3431 1391 Size: 1048 745 247 4072 Fragments arranged by size: 4072 1048 745 247 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2094 2164 928 Size: 971 195 70 4876 Fragments arranged by size: 4876 971 195 70 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4723 136 Size: 107 122 71 631 605 3051 1525 Fragments arranged by size: 3051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 2604 4173 5329 1790 Size: 814 1569 1156 2573 Fragments arranged by size: 2573 1569 1156 814 BmgBI CAC'GTC Cuts at: 1318 2599 3378 1318 Size: 1281 779 4052 Fragments arranged by size: 4052 1281 779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3020 3180 3843 Size: 416 53 83 399 24 1603 160 663 Cuts at: 3843 4801 442 Size: 958 1753 Fragments arranged by size: 1753 1603 958 663 416 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 4772 1239 Size: 1214 2319 2579 Fragments arranged by size: 2579 2319 1214 Bpu10I CC'TnA_GC Cuts at: 3270 3270 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 2972 3124 4237 5102 5400 2291 Size: 681 152 1113 865 298 3003 Fragments arranged by size: 3003 1113 865 681 298 152 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 2817 6106 2817 Size: 3289 2823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 3919 4301 4936 Size: 53 83 186 936 558 1828 382 635 Cuts at: 4936 275 Size: 1451 Fragments arranged by size: 1828 1451 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2328 4545 4943 5217 5364 5775 2328 Size: 2217 398 274 147 411 2665 Fragments arranged by size: 2665 2217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2743 2773 1178 Size: 30 165 30 1340 30 4517 Fragments arranged by size: 4517 1340 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6112 BseYI C'CCAG_C Cuts at: 5315 5811 5315 Size: 496 5616 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2604 3279 4173 4258 5329 2439 Size: 165 675 894 85 1071 3222 Fragments arranged by size: 3222 1071 894 675 165 85 BsiWI C'GTAC_G Cuts at: 2135 2135 Size: 6112 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2604 3184 3279 3431 4173 4258 Size: 399 649 165 580 95 152 742 85 Cuts at: 4258 5329 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 649 580 399 165 152 95 85 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3467 844 Size: 2623 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 4756 4943 1700 Size: 110 518 2428 187 2869 Fragments arranged by size: 2869 2428 518 187 110 BsrGI T'GTAC_A Cuts at: 96 2073 96 Size: 1977 4135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2068 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2224 2565 3323 3442 3809 4194 4211 5663 Size: 252 341 758 119 367 385 17 1452 Cuts at: 5663 5749 5761 6107 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 758 385 367 346 341 252 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2266 2918 513 Size: 552 939 262 652 3707 Fragments arranged by size: 3707 939 652 552 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 3105 3628 4422 4442 3105 Size: 523 794 20 4775 Fragments arranged by size: 4775 794 523 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2619 2654 529 Size: 35 2055 35 3987 Fragments arranged by size: 3987 2055 35 35 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2351 2463 Size: 54 1425 50 275 18 216 305 112 Cuts at: 2463 3559 4448 6077 8 Size: 1096 889 1629 43 Fragments arranged by size: 1629 1425 1096 889 305 275 216 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2537 4042 1353 Size: 1184 1505 3423 Fragments arranged by size: 3423 1505 1184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2437 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2655 4175 4772 5559 5827 1239 Size: 393 821 202 1520 597 787 268 1524 Fragments arranged by size: 1524 1520 821 787 597 393 268 202 EcoO109I rG'GnC_Cy Cuts at: 3106 3106 Size: 6112 EcoRI G'AATT_C Cuts at: 2124 3065 3421 3799 2124 Size: 941 356 378 4437 Fragments arranged by size: 4437 941 378 356 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2240 5259 1678 Size: 562 3019 2531 Fragments arranged by size: 3019 2531 562 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2421 2453 4753 4785 4827 Size: 32 135 32 849 32 2300 32 42 Cuts at: 4827 4859 1373 Size: 32 2626 Fragments arranged by size: 2626 2300 849 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3390 3456 3702 669 Size: 988 281 1452 66 246 3079 Fragments arranged by size: 3079 1452 988 281 246 66 HindIII A'AGCT_T Cuts at: 748 2146 748 Size: 1398 4714 HpaI GTT'AAC Cuts at: 3390 3702 3390 Size: 312 5800 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2786 5226 5410 1305 Size: 1481 2440 184 2007 Fragments arranged by size: 2440 2007 1481 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2353 2465 10 Size: 54 1425 343 521 112 3657 Fragments arranged by size: 3657 1425 521 343 112 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2507 4070 4429 4588 518 Size: 648 903 438 1563 359 159 2042 Fragments arranged by size: 2042 1563 903 648 438 359 159 MspA1I CmG'CkG Cuts at: 1606 3528 4205 5353 5598 1606 Size: 1922 677 1148 245 2120 Fragments arranged by size: 2120 1922 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2918 513 Size: 552 939 914 3707 Fragments arranged by size: 3707 939 914 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2068 3470 5015 1132 Size: 97 839 1402 1545 2229 Fragments arranged by size: 2229 1545 1402 839 97 PasI CC'CwG_GG Cuts at: 1348 2011 1348 Size: 663 5449 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2943 1108 Size: 1835 4277 PfoI T'CCnGG_A Cuts at: 3175 3175 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2573 2605 4181 4213 5694 5726 2573 Size: 32 1576 32 1481 32 2959 Fragments arranged by size: 2959 1576 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3106 3106 Size: 6112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3320 3682 3320 Size: 362 5750 PstI C_TGCA'G Cuts at: 830 2475 3464 830 Size: 1645 989 3478 Fragments arranged by size: 3478 1645 989 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 SacI G_AGCT'C Cuts at: 2439 2439 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2217 2471 3347 3460 4595 5276 5467 Size: 219 1172 254 876 113 1135 681 191 Cuts at: 5467 826 Size: 1471 Fragments arranged by size: 1471 1172 1135 876 681 254 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2951 3139 4216 5117 5379 Size: 197 926 363 645 188 1077 901 262 Cuts at: 5379 820 Size: 1553 Fragments arranged by size: 1553 1077 926 901 645 363 262 197 188 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 2795 4036 5 Size: 47 2743 1241 2081 Fragments arranged by size: 2743 2081 1241 47 StuI AGG'CCT Cuts at: 1517 3121 1517 Size: 1604 4508 StyI C'CwwG_G Cuts at: 513 1065 2004 2918 3551 513 Size: 552 939 914 633 3074 Fragments arranged by size: 3074 939 914 633 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2074 2755 4139 4156 4309 4494 4939 Size: 1255 494 681 1384 17 153 185 445 Cuts at: 4939 325 Size: 1498 Fragments arranged by size: 1498 1384 1255 681 494 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 4358 6070 Size: 274 80 33 51 1443 96 2285 1712 Cuts at: 6070 96 Size: 138 Fragments arranged by size: 2285 1712 1443 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 6072 Size: 32 6080 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3055 3448 3055 Size: 393 5719 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2041 2361 2041 Size: 320 5792 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2092 3920 276 Size: 53 83 186 936 558 1828 2468 Fragments arranged by size: 2468 1828 936 558 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI