MAGEA12 ORF clone FHC03334

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03334

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Clone info.

Species

human

Clone Name

pFN21AB9823 Sequence (with vector) Restriction Map

Product ID

FHC03334

Flexi Clone PID

FXC03334

ORF Sequence

DNA sequence (942 bp)
Protein sequence (314 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464617]

Gene

KIBB9823

Symbol

MAGEA12

Alias : CT1.12, MAGE12

Description

melanoma antigen family A12, transcript variant 3

Original Clone ID

cp00392

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 942 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 314 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS76048.1 1.1e-112 100.0 MAGEA12 CCDS76046.1 2.3e-99 88.2 MAGEA2B CCDS76049.1 2.3e-99 88.2 MAGEA2 CCDS76045.1 7.7e-97 85.4 MAGEA3 CCDS76050.1 8.2e-96 84.4 MAGEA6 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR021072 4 97 PF12440 Melanoma associated antigen IPR002190 116 286 PF01454 MAGE protein ProfileScan IPR002190 109 308 PS50838 MAGE protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005358 314 300177 100.0 100.0 NP_001159859 314 300177 100.0 100.0 NP_001159858 314 300177 100.0 100.0 NP_001269431 314 300173 88.2 100.0 XP_016885008 314 300173 88.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9823 Download

>KIBB9823  942 bp
ATGCCACTTGAGCAGAGGAGTCAGCACTGCAAGCCTGAGGAAGGCCTTGAGGCCCAAGGA
GAGGCCCTGGGCTTGGTGGGTGCGCAGGCTCCTGCTACTGAGGAGCAGGAGACTGCCTCC
TCCTCCTCTACTCTAGTGGAAGTCACCCTGCGGGAGGTGCCTGCTGCCGAGTCACCAAGT
CCTCCCCACAGTCCTCAGGGAGCCTCCACCCTCCCCACTACCATCAACTATACTCTCTGG
AGTCAATCCGATGAGGGCTCCAGCAACGAAGAACAGGAAGGGCCAAGCACCTTTCCTGAC
CTGGAGACGAGCTTCCAAGTAGCACTCAGTAGGAAGATGGCTGAGTTGGTTCATTTTCTG
CTCCTCAAGTATCGAGCCAGGGAGCCATTCACAAAGGCAGAAATGCTGGGGAGTGTCATC
AGAAATTTCCAGGACTTCTTTCCTGTGATCTTCAGCAAAGCCTCCGAGTACTTGCAGCTG
GTCTTTGGCATCGAGGTGGTGGAAGTGGTCCGCATCGGCCACTTGTACATCCTTGTCACC
TGCCTGGGCCTCTCCTACGATGGCCTGCTGGGCGACAATCAGATCGTGCCCAAGACAGGC
CTCCTGATAATCGTCCTGGCCATAATCGCAAAAGAGGGCGACTGTGCCCCTGAGGAGAAA
ATCTGGGAGGAGCTGAGTGTGTTGGAGGCATCTGATGGGAGGGAGGACAGTGTCTTTGCG
CATCCCAGGAAGCTGCTCACCCAAGATTTGGTGCAGGAAAACTACCTGGAGTACCGGCAG
GTCCCCGGCAGTGATCCTGCATGCTACGAGTTCCTGTGGGGTCCAAGGGCCCTCGTTGAA
ACCAGCTATGTGAAAGTCCTGCACCATTTGCTAAAGATCAGTGGAGGACCTCACATTTCC
TACCCACCCCTGCATGAATGGGCTTTTAGAGAGGGGGAAGAG

ORF Protein

Cloned ORF protein sequence for pFN21AB9823 Download

>KIBB9823  314 aa
MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPS
PPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFL
LLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVT
CLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFA
HPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHIS
YPPLHEWAFREGEE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9823 Download

>pFN21AB9823  5647 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCACTTGAGCAGAGGAGTCAGCACTGCAAGCC
TGAGGAAGGCCTTGAGGCCCAAGGAGAGGCCCTGGGCTTGGTGGGTGCGCAGGCTCCTGC
TACTGAGGAGCAGGAGACTGCCTCCTCCTCCTCTACTCTAGTGGAAGTCACCCTGCGGGA
GGTGCCTGCTGCCGAGTCACCAAGTCCTCCCCACAGTCCTCAGGGAGCCTCCACCCTCCC
CACTACCATCAACTATACTCTCTGGAGTCAATCCGATGAGGGCTCCAGCAACGAAGAACA
GGAAGGGCCAAGCACCTTTCCTGACCTGGAGACGAGCTTCCAAGTAGCACTCAGTAGGAA
GATGGCTGAGTTGGTTCATTTTCTGCTCCTCAAGTATCGAGCCAGGGAGCCATTCACAAA
GGCAGAAATGCTGGGGAGTGTCATCAGAAATTTCCAGGACTTCTTTCCTGTGATCTTCAG
CAAAGCCTCCGAGTACTTGCAGCTGGTCTTTGGCATCGAGGTGGTGGAAGTGGTCCGCAT
CGGCCACTTGTACATCCTTGTCACCTGCCTGGGCCTCTCCTACGATGGCCTGCTGGGCGA
CAATCAGATCGTGCCCAAGACAGGCCTCCTGATAATCGTCCTGGCCATAATCGCAAAAGA
GGGCGACTGTGCCCCTGAGGAGAAAATCTGGGAGGAGCTGAGTGTGTTGGAGGCATCTGA
TGGGAGGGAGGACAGTGTCTTTGCGCATCCCAGGAAGCTGCTCACCCAAGATTTGGTGCA
GGAAAACTACCTGGAGTACCGGCAGGTCCCCGGCAGTGATCCTGCATGCTACGAGTTCCT
GTGGGGTCCAAGGGCCCTCGTTGAAACCAGCTATGTGAAAGTCCTGCACCATTTGCTAAA
GATCAGTGGAGGACCTCACATTTCCTACCCACCCCTGCATGAATGGGCTTTTAGAGAGGG
GGAAGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT