(Circular) (Six-base) MAPSORT of: pFN21AB9823.seq Check: 5133 from: 1 to: 5647 pFN21AB9823 5647 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2552 2552 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2990 1937 Size: 1053 4594 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2440 3710 5094 5362 1632 Size: 808 1270 1384 268 1917 Fragments arranged by size: 1917 1384 1270 808 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2112 2133 1184 Size: 928 21 4698 Fragments arranged by size: 4698 928 21 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaI G_GGCC'C Cuts at: 2836 2836 Size: 5647 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2428 2956 3182 3334 5443 1493 Size: 935 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 528 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 2968 4423 618 Size: 325 217 627 374 807 1455 1842 Fragments arranged by size: 1842 1455 807 627 374 325 217 BanII G_rGCy'C Cuts at: 1391 2264 2836 2966 1391 Size: 873 572 130 4072 Fragments arranged by size: 4072 873 572 130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3838 3872 1903 Size: 34 50 34 1817 34 3678 Fragments arranged by size: 3678 1817 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2574 4258 136 Size: 107 122 71 631 605 902 1684 1525 Fragments arranged by size: 1684 1525 902 631 605 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2595 2653 2836 3708 4864 1790 Size: 805 58 183 872 1156 2573 Fragments arranged by size: 2573 1156 872 805 183 58 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2248 2263 2327 2792 4307 1239 Size: 1009 15 64 465 1515 2579 Fragments arranged by size: 2579 1515 1009 465 64 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 2072 2354 3772 4637 4935 2033 Size: 39 282 1418 865 298 2745 Fragments arranged by size: 2745 1418 865 298 282 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3454 3836 4471 275 Size: 53 83 186 936 1921 382 635 1451 Fragments arranged by size: 1921 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2113 2116 2119 2120 2357 2672 2687 Size: 849 78 3 3 1 237 315 15 Cuts at: 2687 1186 Size: 4146 Fragments arranged by size: 4146 849 315 237 78 15 3 3 1 BseYI C'CCAG_C Cuts at: 2410 2572 4850 5346 2410 Size: 162 2278 496 2711 Fragments arranged by size: 2711 2278 496 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 2849 1163 Size: 573 1041 72 3961 Fragments arranged by size: 3961 1041 573 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3708 3793 4864 3708 Size: 85 1071 4491 Fragments arranged by size: 4491 1071 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 BsmBI CGTCTCn'nnnn_ Cuts at: 2303 2303 Size: 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2595 2653 2836 2966 3708 3793 Size: 399 474 331 58 183 130 742 85 Cuts at: 3793 4864 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 331 183 130 85 58 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 2773 3002 844 Size: 1708 221 229 3489 Fragments arranged by size: 3489 1708 229 221 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2778 4291 4478 1700 Size: 110 968 1513 187 2869 Fragments arranged by size: 2869 1513 968 187 110 BsrGI T'GTAC_A Cuts at: 96 2529 96 Size: 2433 3214 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 2872 794 Size: 2078 3569 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2199 2655 2040 Size: 159 456 5032 Fragments arranged by size: 5032 456 159 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5095 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2800 3163 3957 3977 2029 Size: 771 363 794 20 3699 Fragments arranged by size: 3699 794 771 363 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2521 2622 3094 Size: 54 1425 50 275 18 691 101 472 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 691 472 275 101 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2937 3577 1353 Size: 1584 640 3423 Fragments arranged by size: 3423 1584 640 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2248 2263 2327 2440 2792 3710 4307 Size: 393 616 15 64 113 352 918 597 Cuts at: 4307 5094 5362 1239 Size: 787 268 1524 Fragments arranged by size: 1524 918 787 616 597 393 352 268 113 64 15 EcoNI CCTnn'n_nnAGG Cuts at: 2043 2156 2043 Size: 113 5534 EcoO109I rG'GnC_Cy Cuts at: 2068 2785 2832 2833 2891 2068 Size: 717 47 1 58 4824 Fragments arranged by size: 4824 717 58 47 1 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 2088 2724 4153 2088 Size: 636 1429 3582 Fragments arranged by size: 3582 1429 636 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 4288 4320 4362 Size: 32 135 32 674 32 2010 32 42 Cuts at: 4362 4394 1373 Size: 32 2626 Fragments arranged by size: 2626 2010 674 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2667 4761 4945 1305 Size: 1362 2094 184 2007 Fragments arranged by size: 2094 2007 1362 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 10 Size: 54 1425 343 792 3033 Fragments arranged by size: 3033 1425 792 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 3605 3964 4123 518 Size: 648 1332 1107 359 159 2042 Fragments arranged by size: 2042 1332 1107 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2482 3063 3740 4888 5133 1606 Size: 876 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 876 677 581 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5095 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2808 3005 4550 1132 Size: 97 1579 197 1545 2229 Fragments arranged by size: 2229 1579 1545 197 97 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 4924 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2752 1108 Size: 1644 4003 PfoI T'CCnGG_A Cuts at: 2433 2433 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 2891 2785 Size: 106 5541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2832 2832 Size: 5647 PstI C_TGCA'G Cuts at: 830 2999 830 Size: 2169 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2482 2482 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 2474 3895 5607 2474 Size: 1421 1712 2514 Fragments arranged by size: 2514 1712 1421 SfcI C'TryA_G Cuts at: 826 1045 2995 4130 4811 5002 826 Size: 219 1950 1135 681 191 1471 Fragments arranged by size: 1950 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 2051 2369 3751 4652 4914 Size: 197 926 69 39 318 1382 901 262 Cuts at: 4914 820 Size: 1553 Fragments arranged by size: 1553 1382 926 901 318 262 197 69 39 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 2808 3005 1132 Size: 1676 197 3774 Fragments arranged by size: 3774 1676 197 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2049 2604 1517 Size: 532 555 4560 Fragments arranged by size: 4560 555 532 StyI C'CwwG_G Cuts at: 513 1065 2059 2828 3086 513 Size: 552 994 769 258 3074 Fragments arranged by size: 3074 994 769 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 2759 3674 3691 3844 4029 4474 Size: 1255 492 687 915 17 153 185 445 Cuts at: 4474 325 Size: 1498 Fragments arranged by size: 1498 1255 915 687 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2529 3893 Size: 274 80 33 51 1443 495 57 1364 Cuts at: 3893 5605 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1364 495 274 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5607 5639 5607 Size: 32 5615 Tth111I GACn'n_nGTC Cuts at: 1316 2714 1316 Size: 1398 4249 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI