PRAMEF3 ORF clone FHC02451

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC02451

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Clone info.

Species

human

Clone Name

pFN21AB7425 Sequence (with vector) Restriction Map

Product ID

FHC02451

Flexi Clone PID

FXC02451

ORF Sequence

DNA sequence (1437 bp)
Protein sequence (479 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464060]

Gene

KIBB7425

Symbol

PRAMEF3

Description

PRAME family member 3

Original Clone ID

oc00639

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1437 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 479 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41255.1 6.5e-127 62.8 PRAMEF10 CCDS41264.1 7.7e-123 61.9 PRAMEF17 CCDS148.1 1.3e-120 61.6 PRAMEF1 CCDS76109.1 1.2e-118 61.0 PRAMEF14 CCDS149.1 2.7e-118 60.8 PRAMEF2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001278648 493 606021 44.9 97.9 XP_011528336 493 606021 44.9 97.9 NP_001305056 493 606021 44.9 97.9 NP_001278646 493 606021 44.9 97.9 NP_001305055 493 606021 44.9 97.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB7425 Download

>KIBB7425  1437 bp
ATGAGCCTACAGGCCCCACGCAGACTCCTGGAGCTGGCAGGGCAGAGCCTGCTGGGGGAC
CAGGCCTTGGCCATCTCCATCTTGGATGAGCTGCCCAGGGAGCTCTTCCCCCCACTGTTC
GTGGAGGCCTTCACTAGCAGACGCTGCGAGGTTCTGAAGGTGATGGTGCAGGCCTGGCCC
TTCCCCTGCCTCCCTCTGGGGTCCCTGATGAAGACGCCTGATCTGGAGATCTTACATTAT
GTAGTGGATGGGATTGATTGCCTGCTTGCCCAAAAGGTTCGCCCCAGGAGGCGGAAACTT
CAAGTGCTGGAATTGCGGGATGTTGATGAGAATTTTTGGACCATATGGTCTGGAGCCAGG
CCCCTGTCCTGCTCCCCAGAGGCCATGAGTAAGAGGCAGACAGTGGAGGACTGTCCAAGG
ACAGGAGAGAAGCAGCCCTTGAAGGTGTTCATGGATGTTTGCCTCAAGGAAAAATTCATG
GATGAAGATCTGAGCTTCTTCTCTGGGTGGGTCCAGCACAGAAGAGGTTCAGTACACCTG
TGCTGTACTAAGGTGGTGAATTATTCAATGAGCATTCTAAATTTCAGAAACATATTGGAA
ACAGTATACCCAGACAGTATCCAAGTGTTGGAAATTTGGAACATGTGCTGGCCGTGTATG
ATAGTAGAGTTTATCCGTTACCTGAGCCAGATGAGGAATCTTCGCAAACTCTTCATCTCC
GATGGCTGTCGTTACCTGCTAAGCTCTGACAGCCAAGAACAGTTAGTTGCTGAATTCAGC
TCTGTGCTCCTCAGGCTGGAGTACCTCCAGATGCTTTATGTAAGAAGGGTCTGCTTCTTC
AGAGGCCACCTGGACCAGCTGATCAGGTGCCTCAGGAGCCCATTGGAGACATTGGCATTA
ACTTATGGCTTCCTAGAAAAAGTGGACTTGAAATGTCTGCCCCGGTACCCAAGTCTCAGT
CAACTGAAGCAGCTGAATCTGAGTCATGGTGCACTGCGCTTCATCCGTCTTGAGCCCCTC
CGAGCTCTGCTAGAGAAAGTTGCTGCCACTCTTCAGACCCTCTTCTTAGTGGACTGTGGG
ATTCGGGACTCCAAACTCAGGGTCATCCTGCCTGCCCTGAGCTGCTGCTCCAACCTCACC
ACTTTCTGTTTTCACGGCAATGACACGTCCATGGATGGTCTGAAGGACCTGCTGCGCCAC
ACAGGCAGGCTGAGCAATTTGAGCCTGGAAACATATCCTGCCCCTCGGGAGAGTCTTGAT
GACAGGGGTCGTGTCATTTCGGAGCTCCTCACCCCACTTCAGGCTGAGCTGATGCGTATA
CTGAGGGAAGTAAGGGAGCCCAAAAGGATCTTCTTTGGTCCGGTGTCCTGCCCTTGCTGT
GGCACGTCGCCCACTGAGCAACTGGAGTTCAATTTTTGCTTGTGGGGAAGGCCTGCC

ORF Protein

Cloned ORF protein sequence for pFN21AB7425 Download

>KIBB7425  479 aa
MSLQAPRRLLELAGQSLLGDQALAISILDELPRELFPPLFVEAFTSRRCEVLKVMVQAWP
FPCLPLGSLMKTPDLEILHYVVDGIDCLLAQKVRPRRRKLQVLELRDVDENFWTIWSGAR
PLSCSPEAMSKRQTVEDCPRTGEKQPLKVFMDVCLKEKFMDEDLSFFSGWVQHRRGSVHL
CCTKVVNYSMSILNFRNILETVYPDSIQVLEIWNMCWPCMIVEFIRYLSQMRNLRKLFIS
DGCRYLLSSDSQEQLVAEFSSVLLRLEYLQMLYVRRVCFFRGHLDQLIRCLRSPLETLAL
TYGFLEKVDLKCLPRYPSLSQLKQLNLSHGALRFIRLEPLRALLEKVAATLQTLFLVDCG
IRDSKLRVILPALSCCSNLTTFCFHGNDTSMDGLKDLLRHTGRLSNLSLETYPAPRESLD
DRGRVISELLTPLQAELMRILREVREPKRIFFGPVSCPCCGTSPTEQLEFNFCLWGRPA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB7425 Download

>pFN21AB7425  6142 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCCTACAGGCCCCACGCAGACTCCTGGAGCT
GGCAGGGCAGAGCCTGCTGGGGGACCAGGCCTTGGCCATCTCCATCTTGGATGAGCTGCC
CAGGGAGCTCTTCCCCCCACTGTTCGTGGAGGCCTTCACTAGCAGACGCTGCGAGGTTCT
GAAGGTGATGGTGCAGGCCTGGCCCTTCCCCTGCCTCCCTCTGGGGTCCCTGATGAAGAC
GCCTGATCTGGAGATCTTACATTATGTAGTGGATGGGATTGATTGCCTGCTTGCCCAAAA
GGTTCGCCCCAGGAGGCGGAAACTTCAAGTGCTGGAATTGCGGGATGTTGATGAGAATTT
TTGGACCATATGGTCTGGAGCCAGGCCCCTGTCCTGCTCCCCAGAGGCCATGAGTAAGAG
GCAGACAGTGGAGGACTGTCCAAGGACAGGAGAGAAGCAGCCCTTGAAGGTGTTCATGGA
TGTTTGCCTCAAGGAAAAATTCATGGATGAAGATCTGAGCTTCTTCTCTGGGTGGGTCCA
GCACAGAAGAGGTTCAGTACACCTGTGCTGTACTAAGGTGGTGAATTATTCAATGAGCAT
TCTAAATTTCAGAAACATATTGGAAACAGTATACCCAGACAGTATCCAAGTGTTGGAAAT
TTGGAACATGTGCTGGCCGTGTATGATAGTAGAGTTTATCCGTTACCTGAGCCAGATGAG
GAATCTTCGCAAACTCTTCATCTCCGATGGCTGTCGTTACCTGCTAAGCTCTGACAGCCA
AGAACAGTTAGTTGCTGAATTCAGCTCTGTGCTCCTCAGGCTGGAGTACCTCCAGATGCT
TTATGTAAGAAGGGTCTGCTTCTTCAGAGGCCACCTGGACCAGCTGATCAGGTGCCTCAG
GAGCCCATTGGAGACATTGGCATTAACTTATGGCTTCCTAGAAAAAGTGGACTTGAAATG
TCTGCCCCGGTACCCAAGTCTCAGTCAACTGAAGCAGCTGAATCTGAGTCATGGTGCACT
GCGCTTCATCCGTCTTGAGCCCCTCCGAGCTCTGCTAGAGAAAGTTGCTGCCACTCTTCA
GACCCTCTTCTTAGTGGACTGTGGGATTCGGGACTCCAAACTCAGGGTCATCCTGCCTGC
CCTGAGCTGCTGCTCCAACCTCACCACTTTCTGTTTTCACGGCAATGACACGTCCATGGA
TGGTCTGAAGGACCTGCTGCGCCACACAGGCAGGCTGAGCAATTTGAGCCTGGAAACATA
TCCTGCCCCTCGGGAGAGTCTTGATGACAGGGGTCGTGTCATTTCGGAGCTCCTCACCCC
ACTTCAGGCTGAGCTGATGCGTATACTGAGGGAAGTAAGGGAGCCCAAAAGGATCTTCTT
TGGTCCGGTGTCCTGCCCTTGCTGTGGCACGTCGCCCACTGAGCAACTGGAGTTCAATTT
TTGCTTGTGGGGAAGGCCTGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT