(Circular) (Six-base) MAPSORT of: pFN21AB7425.seq Check: 8103 from: 1 to: 6142 pFN21AB7425 6142 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 3322 3485 1937 Size: 673 712 163 4594 Fragments arranged by size: 4594 712 673 163 Acc65I G'GTAC_C Cuts at: 2949 3463 2949 Size: 514 5628 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 2827 2990 3041 3206 3287 4205 5589 Size: 548 647 163 51 165 81 918 1384 Cuts at: 5589 5857 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 918 647 548 268 165 163 81 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 2646 3168 5041 6018 1203 Size: 1443 522 1873 977 1327 Fragments arranged by size: 1873 1443 1327 977 522 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2053 2148 3885 5457 2053 Size: 95 1737 1572 2738 Fragments arranged by size: 2738 1737 1572 95 ApaLI G'TGCA_C Cuts at: 2994 4199 5355 2994 Size: 1205 1156 3781 Fragments arranged by size: 3781 1205 1156 ApoI r'AATT_y Cuts at: 1493 2335 2477 2584 2637 2777 3451 3677 3829 Size: 842 142 107 53 140 674 226 152 Cuts at: 3829 5938 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 842 674 226 152 142 140 107 53 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3249 3467 1870 Size: 73 1306 218 4545 Fragments arranged by size: 4545 1306 218 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2941 2974 2941 Size: 33 6109 BamHI G'GATC_C Cuts at: 3472 3839 3472 Size: 367 5775 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2871 2949 3463 4918 618 Size: 325 217 627 1084 78 514 1455 1842 Fragments arranged by size: 1842 1455 1084 627 514 325 217 78 BanII G_rGCy'C Cuts at: 1391 2109 2885 3021 3031 3291 3345 3461 1391 Size: 718 776 136 10 260 54 116 4072 Fragments arranged by size: 4072 776 718 260 136 116 54 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 928 Size: 971 323 4848 Fragments arranged by size: 4848 971 323 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 4034 5244 2633 Size: 1401 1210 3531 Fragments arranged by size: 3531 1401 1210 BclI T'GATC_A Cuts at: 1548 2865 1548 Size: 1317 4825 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3207 4753 136 Size: 107 122 71 631 605 1535 1546 1525 Fragments arranged by size: 1546 1535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2232 2491 6137 2232 Size: 259 3646 2237 Fragments arranged by size: 3646 2237 259 BlpI GC'TnA_GC Cuts at: 2744 3215 3309 3559 2744 Size: 471 94 250 5327 Fragments arranged by size: 5327 471 250 94 Bme1580I G_kGCm'C Cuts at: 1790 2998 4203 5359 1790 Size: 1208 1205 1156 2573 Fragments arranged by size: 2573 1208 1205 1156 BmgBI CAC'GTC Cuts at: 1318 3171 3390 1318 Size: 1853 219 4070 Fragments arranged by size: 4070 1853 219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2249 2376 2795 2822 3428 4802 1239 Size: 815 195 127 419 27 606 1374 2579 Fragments arranged by size: 2579 1374 815 606 419 195 127 27 Bpu10I CC'TnA_GC Cuts at: 2687 3122 2687 Size: 435 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2453 3035 4267 5132 5430 2453 Size: 582 1232 865 298 3165 Fragments arranged by size: 3165 1232 865 582 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 2490 6136 2490 Size: 3646 2496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2219 3949 4331 4966 Size: 53 83 186 936 686 1730 382 635 Cuts at: 4966 275 Size: 1451 Fragments arranged by size: 1730 1451 936 686 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3364 4575 4973 5247 5394 5805 3364 Size: 1211 398 274 147 411 3701 Fragments arranged by size: 3701 1211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2013 2043 1178 Size: 30 165 30 610 30 5277 Fragments arranged by size: 5277 610 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2783 3281 1186 Size: 1597 498 4047 Fragments arranged by size: 4047 1597 498 BseYI C'CCAG_C Cuts at: 2056 5345 5841 2056 Size: 3289 496 2357 Fragments arranged by size: 3289 2357 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 1163 Size: 573 456 5113 Fragments arranged by size: 5113 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 2793 2998 3031 3291 4203 4288 5359 2109 Size: 684 205 33 260 912 85 1071 2892 Fragments arranged by size: 2892 1071 912 684 260 205 85 33 BsmI GAATG_Cn' Cuts at: 2577 3653 3746 2577 Size: 1076 93 4973 Fragments arranged by size: 4973 1076 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2793 2885 2998 3021 3031 3291 Size: 399 319 684 92 113 23 10 260 Cuts at: 3291 3345 3461 4203 4288 5359 1391 Size: 54 116 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 684 399 319 260 116 113 92 85 54 23 10 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2748 3201 3497 844 Size: 1904 453 296 3489 Fragments arranged by size: 3489 1904 453 296 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3169 4637 4811 65 Size: 1183 1921 1468 174 1396 Fragments arranged by size: 1921 1468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 2148 794 Size: 1259 95 4788 Fragments arranged by size: 4788 1259 95 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2232 2491 3351 3472 3839 4224 4241 5693 Size: 260 259 860 121 367 385 17 1452 Cuts at: 5693 5779 5791 6137 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 860 385 367 346 260 259 121 86 17 12 BstZ17I GTA'TAC Cuts at: 2611 3323 2611 Size: 712 5430 Bsu36I CC'TnA_GG Cuts at: 2795 2876 2795 Size: 81 6061 BtgI C'CryG_G Cuts at: 513 1065 3174 513 Size: 552 2109 3481 Fragments arranged by size: 3481 2109 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 2996 3658 4452 4472 2996 Size: 662 794 20 4666 Fragments arranged by size: 4666 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2350 5149 809 Size: 507 1034 2799 1802 Fragments arranged by size: 2799 1802 1034 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2654 3589 Size: 54 1425 50 275 18 243 581 935 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 935 889 581 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2521 2720 3060 3071 4072 1353 Size: 761 407 199 340 11 1001 3423 Fragments arranged by size: 3423 1001 761 407 340 199 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2311 4729 5103 5249 387 Size: 1064 138 722 2418 374 146 1280 Fragments arranged by size: 2418 1280 1064 722 374 146 138 EcoICRI GAG'CTC Cuts at: 2107 3029 3289 2107 Size: 922 260 4960 Fragments arranged by size: 4960 922 260 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2180 2249 2376 2795 2822 2827 Size: 393 422 126 69 127 419 27 5 Cuts at: 2827 2990 3041 3206 3287 3428 4205 4802 5589 Size: 163 51 165 81 141 777 597 787 Cuts at: 5589 5857 1239 Size: 268 1524 Fragments arranged by size: 1524 787 777 597 422 419 393 268 165 163 141 127 126 81 69 51 27 5 EcoO109I rG'GnC_Cy Cuts at: 2017 2205 2364 3190 2017 Size: 188 159 826 4969 Fragments arranged by size: 4969 826 188 159 EcoRI G'AATT_C Cuts at: 2777 3451 3829 2777 Size: 674 378 5090 Fragments arranged by size: 5090 674 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2209 2241 2686 2718 4783 Size: 32 135 32 637 32 445 32 2065 Cuts at: 4783 4815 4857 4889 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2065 637 445 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2966 3486 3732 669 Size: 988 281 1028 520 246 3079 Fragments arranged by size: 3079 1028 988 520 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 2953 3467 2953 Size: 514 5628 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 3159 5256 5440 1305 Size: 1308 546 2097 184 2007 Fragments arranged by size: 2097 2007 1308 546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 10 Size: 54 1425 343 243 4077 Fragments arranged by size: 4077 1425 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 3162 3173 4100 4459 4618 518 Size: 648 1996 11 927 359 159 2042 Fragments arranged by size: 2042 1996 927 648 359 159 11 MspA1I CmG'CkG Cuts at: 1606 2863 2977 3558 4235 5383 5628 1606 Size: 1257 114 581 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 677 581 245 114 NcoI C'CATG_G Cuts at: 513 1065 3174 513 Size: 552 2109 3481 Fragments arranged by size: 3481 2109 552 NdeI CA'TA_TG Cuts at: 387 2348 387 Size: 1961 4181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2650 3500 5045 1132 Size: 97 1421 850 1545 2229 Fragments arranged by size: 2229 1545 1421 850 97 PasI CC'CwG_GG Cuts at: 1348 2100 1348 Size: 752 5390 PciI A'CATG_T Cuts at: 2646 5041 2646 Size: 2395 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 2632 1108 Size: 963 561 4618 Fragments arranged by size: 4618 963 561 PfoI T'CCnGG_A Cuts at: 2031 2031 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 3190 2205 Size: 985 5157 PshAI GACnn'nnGTC Cuts at: 1613 3270 1613 Size: 1657 4485 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2863 2977 2863 Size: 114 6028 SacI G_AGCT'C Cuts at: 2109 3031 3291 2109 Size: 922 260 4960 Fragments arranged by size: 4960 922 260 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SanDI GG'GwC_CC Cuts at: 2205 2205 Size: 6142 SapI GCTCTTCn'nnn_ Cuts at: 2114 2114 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2012 3490 4625 5306 5497 826 Size: 219 967 1478 1135 681 191 1471 Fragments arranged by size: 1478 1471 1135 967 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2468 3014 4246 5147 5409 820 Size: 197 926 525 546 1232 901 262 1553 Fragments arranged by size: 1553 1232 926 901 546 525 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2069 2132 2177 3436 1517 Size: 552 63 45 1259 4223 Fragments arranged by size: 4223 1259 552 63 45 StyI C'CwwG_G Cuts at: 513 1065 2070 2420 3174 3581 513 Size: 552 1005 350 754 407 3074 Fragments arranged by size: 3074 1005 754 552 407 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2499 4169 4186 4339 4524 4969 325 Size: 1255 919 1670 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1255 919 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2536 2549 4388 Size: 274 80 33 51 1443 559 13 1839 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1839 1712 1443 559 274 138 80 51 33 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 6102 6134 2013 Size: 32 4057 32 2021 Fragments arranged by size: 4057 2021 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 3403 2083 Size: 1320 4822 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 2450 2530 4271 2450 Size: 80 1741 4321 Fragments arranged by size: 4321 1741 80 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI