RUFY2 ORF clone FHC00857

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00857

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Clone info.

Species

human

Clone Name

pFN21ASDA1537 Sequence (with vector) Restriction Map

Product ID

FHC00857

Flexi Clone PID

FXC00857

ORF Sequence

DNA sequence (1770 bp)
Protein sequence (590 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384229]

Gene

KIAA1537

Symbol

RUFY2

Alias : RABIP4R, ZFYVE13

Description

RUN and FYVE domain containing 2

Original Clone ID

fj02581s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1770 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 590 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS81470.1 1.3e-88 100.0 RUFY2 CCDS41534.1 1.4e-88 100.0 RUFY2 CCDS34312.1 1.2e-55 62.9 RUFY1 CCDS4445.2 1.4e-55 62.9 RUFY1 CCDS60544.1 3.6e-48 100.0 RUFY2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004012 29 151 PF02759 RUN IPR000306 520 580 PF01363 Zinc finger HMMSmart IPR004012 89 151 SM00593 RUN IPR000306 516 583 SM00064 Zinc finger ProfileScan IPR004012 21 153 PS50826 RUN IPR017455 524 582 PS50178 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005270012 590 610328 100.0 100.0 NP_001317032 606 610328 100.0 100.0 XP_011538244 606 610328 100.0 100.0 NP_060457 641 610328 100.0 100.0 XP_005270014 572 610328 94.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1537 Download

>KIAA1537  1770 bp
ATGGCTAAACTGAGTATCAAAGGACTCATTGAATCTGCTCTGAGCTTTGGCCGCACTTTG
GATTCTGACTATCCCCCCTTGCAGCAATTCTTTGTTGTTATGGAACATTGCCTGAAACAC
GGTCTTAAAGTAAGAAAATCATTTTTGAGTTACAACAAAACCATCTGGGGCCCTTTGGAA
CTGGTGGAGAAGCTGTACCCCGAAGCAGAGGAAATAGGAGCTAGTGTCCGGGATCTACCT
GGTCTGAAGACCCCTCTGGGTCGAGCAAGAGCGTGGCTTCGATTAGCCCTCATGCAAAAA
AAAATGGCCGATTACTTACGTTGCTTAATTATTCAGAGGGATCTCTTGAGTGAGTTTTAT
GAGTATCACGCACTAATGATGGAAGAAGAAGGAGCAGTAATTGTTGGGCTGCTGGTTGGC
CTGAATGTGATCGATGCTAATCTGTGTGTGAAGGGAGAGGATTTAGACTCACAAGTTGGA
GTGATTGATTTTTCTATGTATTTAAAGAATGAAGAAGATATTGGAAATAAAGAAAGGAAT
GTTCAAATTGCTGCCATATTAGACCAAAAGAATTATGTTGAAGAATTAAATAGACAACTG
AACAGCACAGTCAGCAGCCTCCATTCAAGAGTTGATTCATTAGAAAAGTCAAATACTAAG
CTGATTGAAGAGTTAGCAATAGCAAAGAATAACATCATTAAACTCCAGGAAGAAAATCAT
CAATTACGAAGTGAAAATAAATTGATTTTAATGAAAACACAGCAGCACCTAGAGGTTACC
AAAGTAGATGTGGAAACTGAGCTTCAAACATATAAGCATTCTCGTCAGGGGCTAGATGAA
ATGTACAATGAAGCCAGAAGGCAGCTTCGAGATGAATCTCAGTTACGACAGGATGTAGAG
AATGAGCTAGCAGTACAAGTTAGTATGAAGCATGAGATTGAACTTGCCATGAAGTTGCTG
GAGAAAGATATCCATGAGAAACAAGATACTCTGATAGGCCTTCGACAACAACTAGAGGAA
GTTAAAGCAATTAACATAGAGATGTATCAAAAGTTGCAGGGTTCTGAAGATGGCTTGAAA
GAAAAAAATGAAATAATTGCCCGACTAGAAGAAAAAACCAATAAAATTACTGCAGCCATG
AGGCAGCTGGAACAAAGATTGCAGCAAGCAGAGAAGGCGCAAATGGAAGCTGAAGATGAG
GATGAGAAATATCTACAAGAATGTCTCAGTAAATCTGATAGTCTGCAGAAACAAATCTCC
CAAAAGGAGAAACAGCTGGTGCAACTGGAAACTGACTTGAAGATTGAGAAGGAATGGAGG
CAGACTTTGCAGGAAGATCTTCAAAAGGAGAAAGATGCCTTATCTCATCTTAGAAATGAG
ACTCAACAAATCATTAGTCTTAAAAAAGAGTTCCTTAACCTCCAGGATGAAAATCAGCAG
TTGAAAAAAATATATCATGAACAAGAGCAAGCTCTTCAAGAACTCGGCAACAAGCTTAGC
GAATCAAAACTTAAAATTGAAGACATAAAAGAAGCCAACAAAGCATTGCAGGGACTGGTT
TGGCTGAAAGACAAAGAAGCAACACATTGTAAACTTTGTGAAAAGGAATTCTCACTCTCT
AAGAGAAAGCACCACTGTAGAAATTGTGGGGAAATTTTCTGTAATGCCTGCTCTGACAAC
GAACTACCTTTGCCTTCTTCACCAAAACCAGTACGGGTTTGTGATTCCTGTCATGCATTG
CTCATTCAGAGATGCTCATCTAACTTGCCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1537 Download

>KIAA1537  590 aa
MAKLSIKGLIESALSFGRTLDSDYPPLQQFFVVMEHCLKHGLKVRKSFLSYNKTIWGPLE
LVEKLYPEAEEIGASVRDLPGLKTPLGRARAWLRLALMQKKMADYLRCLIIQRDLLSEFY
EYHALMMEEEGAVIVGLLVGLNVIDANLCVKGEDLDSQVGVIDFSMYLKNEEDIGNKERN
VQIAAILDQKNYVEELNRQLNSTVSSLHSRVDSLEKSNTKLIEELAIAKNNIIKLQEENH
QLRSENKLILMKTQQHLEVTKVDVETELQTYKHSRQGLDEMYNEARRQLRDESQLRQDVE
NELAVQVSMKHEIELAMKLLEKDIHEKQDTLIGLRQQLEEVKAINIEMYQKLQGSEDGLK
EKNEIIARLEEKTNKITAAMRQLEQRLQQAEKAQMEAEDEDEKYLQECLSKSDSLQKQIS
QKEKQLVQLETDLKIEKEWRQTLQEDLQKEKDALSHLRNETQQIISLKKEFLNLQDENQQ
LKKIYHEQEQALQELGNKLSESKLKIEDIKEANKALQGLVWLKDKEATHCKLCEKEFSLS
KRKHHCRNCGEIFCNACSDNELPLPSSPKPVRVCDSCHALLIQRCSSNLP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1537 Download

>pFN21ASDA1537  6499 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCTAAACTGAGTAT
CAAAGGACTCATTGAATCTGCTCTGAGCTTTGGCCGCACTTTGGATTCTGACTATCCCCC
CTTGCAGCAATTCTTTGTTGTTATGGAACATTGCCTGAAACACGGTCTTAAAGTAAGAAA
ATCATTTTTGAGTTACAACAAAACCATCTGGGGCCCTTTGGAACTGGTGGAGAAGCTGTA
CCCCGAAGCAGAGGAAATAGGAGCTAGTGTCCGGGATCTACCTGGTCTGAAGACCCCTCT
GGGTCGAGCAAGAGCGTGGCTTCGATTAGCCCTCATGCAAAAAAAAATGGCCGATTACTT
ACGTTGCTTAATTATTCAGAGGGATCTCTTGAGTGAGTTTTATGAGTATCACGCACTAAT
GATGGAAGAAGAAGGAGCAGTAATTGTTGGGCTGCTGGTTGGCCTGAATGTGATCGATGC
TAATCTGTGTGTGAAGGGAGAGGATTTAGACTCACAAGTTGGAGTGATTGATTTTTCTAT
GTATTTAAAGAATGAAGAAGATATTGGAAATAAAGAAAGGAATGTTCAAATTGCTGCCAT
ATTAGACCAAAAGAATTATGTTGAAGAATTAAATAGACAACTGAACAGCACAGTCAGCAG
CCTCCATTCAAGAGTTGATTCATTAGAAAAGTCAAATACTAAGCTGATTGAAGAGTTAGC
AATAGCAAAGAATAACATCATTAAACTCCAGGAAGAAAATCATCAATTACGAAGTGAAAA
TAAATTGATTTTAATGAAAACACAGCAGCACCTAGAGGTTACCAAAGTAGATGTGGAAAC
TGAGCTTCAAACATATAAGCATTCTCGTCAGGGGCTAGATGAAATGTACAATGAAGCCAG
AAGGCAGCTTCGAGATGAATCTCAGTTACGACAGGATGTAGAGAATGAGCTAGCAGTACA
AGTTAGTATGAAGCATGAGATTGAACTTGCCATGAAGTTGCTGGAGAAAGATATCCATGA
GAAACAAGATACTCTGATAGGCCTTCGACAACAACTAGAGGAAGTTAAAGCAATTAACAT
AGAGATGTATCAAAAGTTGCAGGGTTCTGAAGATGGCTTGAAAGAAAAAAATGAAATAAT
TGCCCGACTAGAAGAAAAAACCAATAAAATTACTGCAGCCATGAGGCAGCTGGAACAAAG
ATTGCAGCAAGCAGAGAAGGCGCAAATGGAAGCTGAAGATGAGGATGAGAAATATCTACA
AGAATGTCTCAGTAAATCTGATAGTCTGCAGAAACAAATCTCCCAAAAGGAGAAACAGCT
GGTGCAACTGGAAACTGACTTGAAGATTGAGAAGGAATGGAGGCAGACTTTGCAGGAAGA
TCTTCAAAAGGAGAAAGATGCCTTATCTCATCTTAGAAATGAGACTCAACAAATCATTAG
TCTTAAAAAAGAGTTCCTTAACCTCCAGGATGAAAATCAGCAGTTGAAAAAAATATATCA
TGAACAAGAGCAAGCTCTTCAAGAACTCGGCAACAAGCTTAGCGAATCAAAACTTAAAAT
TGAAGACATAAAAGAAGCCAACAAAGCATTGCAGGGACTGGTTTGGCTGAAAGACAAAGA
AGCAACACATTGTAAACTTTGTGAAAAGGAATTCTCACTCTCTAAGAGAAAGCACCACTG
TAGAAATTGTGGGGAAATTTTCTGTAATGCCTGCTCTGACAACGAACTACCTTTGCCTTC
TTCACCAAAACCAGTACGGGTTTGTGATTCCTGTCATGCATTGCTCATTCAGAGATGCTC
ATCTAACTTGCCCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT