(Circular) (Six-base) MAPSORT of: pFN21ASDA1537.seq Check: 5414 from: 1 to: 6499 pFN21ASDA1537 6499 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4309 278 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3842 1937 Size: 1905 4594 Acc65I G'GTAC_C Cuts at: 3820 3820 Size: 6499 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 3108 3234 4562 5946 6214 1632 Size: 656 820 126 1328 1384 268 1917 Fragments arranged by size: 1917 1384 1328 820 656 268 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6302 AflIII A'CryG_T Cuts at: 1203 5398 6375 1203 Size: 4195 977 1327 Fragments arranged by size: 4195 1327 977 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 5228 5228 Size: 6499 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6499 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6499 AlwNI CAG_nnn'CTG Cuts at: 3577 4242 5814 3577 Size: 665 1572 4262 Fragments arranged by size: 4262 1572 665 ApaI G_GGCC'C Cuts at: 2195 2195 Size: 6499 ApaLI G'TGCA_C Cuts at: 4556 5712 4556 Size: 1156 5343 ApoI r'AATT_y Cuts at: 1493 3629 3675 3808 4034 4186 6295 1493 Size: 2136 46 133 226 152 2109 1697 Fragments arranged by size: 2136 2109 1697 226 152 133 46 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 3824 1870 Size: 73 1881 4545 Fragments arranged by size: 4545 1881 73 BamHI G'GATC_C Cuts at: 3829 4196 3829 Size: 367 6132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3820 5275 618 Size: 325 217 627 2033 1455 1842 Fragments arranged by size: 2033 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2195 3818 1391 Size: 804 1623 4072 Fragments arranged by size: 4072 1623 804 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 3549 928 Size: 971 377 1273 3878 Fragments arranged by size: 3878 1273 971 377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4690 4724 1903 Size: 34 2753 34 3678 Fragments arranged by size: 3678 2753 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4391 5601 4391 Size: 1210 5289 BclI T'GATC_A Cuts at: 1548 1548 Size: 6499 BfrBI ATG'CAT Cuts at: 3758 3758 Size: 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5110 136 Size: 107 122 71 631 605 3438 1525 Fragments arranged by size: 3438 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3358 6494 3358 Size: 3136 3363 BlpI GC'TnA_GC Cuts at: 3518 3916 3518 Size: 398 6101 Bme1580I G_kGCm'C Cuts at: 1790 2195 4560 5716 1790 Size: 405 2365 1156 2573 Fragments arranged by size: 2573 2365 1156 405 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4230 5188 442 Size: 416 53 83 399 24 2813 958 1753 Fragments arranged by size: 2813 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2933 1056 Size: 1877 4622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 3002 3428 5159 1239 Size: 1472 291 426 1731 2579 Fragments arranged by size: 2579 1731 1472 426 291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2389 4624 5489 5787 2389 Size: 2235 865 298 3101 Fragments arranged by size: 3101 2235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5162 882 Size: 617 3663 2219 Fragments arranged by size: 3663 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3796 4290 493 Size: 711 2592 494 2702 Fragments arranged by size: 2702 2592 711 494 BsaBI GATnn'nnATC Cuts at: 2460 3453 3778 6493 2460 Size: 993 325 2715 2466 Fragments arranged by size: 2715 2466 993 325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4306 4688 5323 275 Size: 53 83 186 936 2773 382 635 1451 Fragments arranged by size: 2773 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4932 5330 5604 5751 6162 4932 Size: 398 274 147 411 5269 Fragments arranged by size: 5269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 2232 2262 1178 Size: 30 165 30 798 30 1 30 5415 Fragments arranged by size: 5415 798 165 30 30 30 30 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6499 BseYI C'CCAG_C Cuts at: 5702 6198 5702 Size: 496 6003 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5926 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3949 4710 5738 1540 Size: 275 186 1948 761 1028 2301 Fragments arranged by size: 2301 1948 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4560 4645 5716 4560 Size: 85 1071 5343 Fragments arranged by size: 5343 1071 85 BsmI GAATG_Cn' Cuts at: 2839 4010 4103 2839 Size: 1171 93 5235 Fragments arranged by size: 5235 1171 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 3818 4560 4645 5716 1391 Size: 399 405 1623 742 85 1071 2174 Fragments arranged by size: 2174 1623 1071 742 405 399 85 BspEI T'CCGG_A Cuts at: 6162 6162 Size: 6499 BspHI T'CATG_A Cuts at: 3478 4388 6118 3478 Size: 910 1730 3859 Fragments arranged by size: 3859 1730 910 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3854 844 Size: 3010 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4386 1394 Size: 429 2563 3507 Fragments arranged by size: 3507 2563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2128 3566 3758 4994 5168 65 Size: 1183 880 1438 192 1236 174 1396 Fragments arranged by size: 1438 1396 1236 1183 880 192 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5143 5330 1700 Size: 110 3333 187 2869 Fragments arranged by size: 3333 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2865 96 Size: 2769 3730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6499 BssSI C'ACGA_G Cuts at: 4559 5571 4559 Size: 1012 5487 BstAPI GCAn_nnn'nTGC Cuts at: 794 3349 794 Size: 2555 3944 BstBI TT'CG_AA Cuts at: 2007 5303 2007 Size: 3296 3203 BstEII G'GTnAC_C Cuts at: 1184 2797 1184 Size: 1613 4886 BstYI r'GATC_y Cuts at: 1972 2254 2362 3358 3829 4196 4581 4598 6050 Size: 282 108 996 471 367 385 17 1452 Cuts at: 6050 6136 6148 6494 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 996 471 385 367 346 282 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4990 Fragments arranged by size: 4990 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5760 Fragments arranged by size: 5760 683 56 BtsI GCAGTG_nn' Cuts at: 4015 4809 4829 4015 Size: 794 20 5685 Fragments arranged by size: 5685 794 20 ClaI AT'CG_AT Cuts at: 1554 2454 1554 Size: 900 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6464 DraI TTT'AAA Cuts at: 2526 3803 2526 Size: 1277 5222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2328 3946 Size: 54 1425 50 275 18 241 257 1618 Cuts at: 3946 4835 6464 8 Size: 889 1629 43 Fragments arranged by size: 1629 1618 1425 889 275 257 241 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3946 1537 Size: 275 2134 4090 Fragments arranged by size: 4090 2134 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 3501 4429 1353 Size: 1332 816 928 3423 Fragments arranged by size: 3423 1332 928 816 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5086 5460 5606 387 Size: 1064 138 3497 374 146 1280 Fragments arranged by size: 3497 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2711 3002 3108 3234 3428 4562 Size: 393 656 423 291 106 126 194 1134 Cuts at: 4562 5159 5946 6214 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1134 787 656 597 423 393 291 268 194 126 106 EcoO109I rG'GnC_Cy Cuts at: 2191 2192 2191 Size: 1 6498 EcoRI G'AATT_C Cuts at: 3629 3808 4186 3629 Size: 179 378 5942 Fragments arranged by size: 5942 378 179 EcoRV GAT'ATC Cuts at: 2992 2992 Size: 6499 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3452 3452 Size: 6499 FspI TGC'GCA Cuts at: 5005 5005 Size: 6499 HaeII r_GCGC'y Cuts at: 1678 5646 1678 Size: 3968 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2232 2264 2295 2327 2474 Size: 32 135 32 660 32 31 32 147 Cuts at: 2474 2506 3368 3400 5140 5172 5214 5246 1373 Size: 32 862 32 1740 32 42 32 2626 Fragments arranged by size: 2626 1740 862 660 147 135 42 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3843 4089 669 Size: 988 281 1905 246 3079 Fragments arranged by size: 3079 1905 988 281 246 HindIII A'AGCT_T Cuts at: 748 3515 748 Size: 2767 3732 HpaI GTT'AAC Cuts at: 4089 4089 Size: 6499 KpnI G_GTAC'C Cuts at: 3824 3824 Size: 6499 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 6375 6375 Size: 6499 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2479 5613 5797 1305 Size: 1174 3134 184 2007 Fragments arranged by size: 3134 2007 1174 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4677 Fragments arranged by size: 4677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3062 4457 4816 4975 518 Size: 648 1896 1395 359 159 2042 Fragments arranged by size: 2042 1896 1395 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3169 3298 3915 4592 5740 5985 1606 Size: 1563 129 617 677 1148 245 2120 Fragments arranged by size: 2120 1563 1148 677 617 245 129 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4990 Fragments arranged by size: 4990 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6499 NheI G'CTAG_C Cuts at: 1052 2929 1052 Size: 1877 4622 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NsiI A_TGCA'T Cuts at: 3760 3760 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 3857 5402 1132 Size: 97 2628 1545 2229 Fragments arranged by size: 2628 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6499 PciI A'CATG_T Cuts at: 5398 5398 Size: 6499 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6499 PfoI T'CCnGG_A Cuts at: 2250 2727 3444 2250 Size: 477 717 5305 Fragments arranged by size: 5305 717 477 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6499 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PspOMI G'GGCC_C Cuts at: 2191 2191 Size: 6499 PstI C_TGCA'G Cuts at: 830 3157 3270 3851 830 Size: 2327 113 581 3478 Fragments arranged by size: 3478 2327 581 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 3169 3298 3169 Size: 129 6370 SalI G'TCGA_C Cuts at: 1936 3841 1936 Size: 1905 4594 SapI GCTCTTCn'nnn_ Cuts at: 3501 3501 Size: 6499 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 4747 6459 4747 Size: 1712 4787 SexAI A'CCwGG_T Cuts at: 2260 2260 Size: 6499 SfcI C'TryA_G Cuts at: 826 1045 3153 3266 3658 3847 4982 5663 5854 Size: 219 2108 113 392 189 1135 681 191 Cuts at: 5854 826 Size: 1471 Fragments arranged by size: 2108 1471 1135 681 392 219 191 189 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 3826 1872 Size: 1954 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2368 4603 5504 5766 820 Size: 197 926 425 2235 901 262 1553 Fragments arranged by size: 2235 1553 926 901 425 262 197 SnaBI TAC'GTA Cuts at: 493 3796 4290 493 Size: 3303 494 2702 Fragments arranged by size: 3303 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 3857 1132 Size: 2725 3774 SspI AAT'ATT Cuts at: 5 52 4423 5 Size: 47 4371 2081 Fragments arranged by size: 4371 2081 47 StuI AGG'CCT Cuts at: 1517 3021 1517 Size: 1504 4995 StyI C'CwwG_G Cuts at: 513 1065 2022 3938 513 Size: 552 957 1916 3074 Fragments arranged by size: 3074 1916 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4526 4543 4696 4881 5326 325 Size: 1255 2946 17 153 185 445 1498 Fragments arranged by size: 2946 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2865 2935 4745 Size: 274 80 33 51 1443 888 70 1810 Cuts at: 4745 6457 96 Size: 1712 138 Fragments arranged by size: 1810 1712 1443 888 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6459 6491 6459 Size: 32 6467 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6499 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2977 3166 2809 Size: 168 189 6142 Fragments arranged by size: 6142 189 168 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6499 XmaI C'CCGG_G Cuts at: 1870 3824 1870 Size: 1954 4545 XmnI GAAnn'nnTTC Cuts at: 3360 4628 3360 Size: 1268 5231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4307 276 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI