RNF8 ORF clone FHC00589

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00589

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Clone info.

Species

human

Clone Name

pFN21ASDA0646 Sequence (with vector) Restriction Map

Product ID

FHC00589

Flexi Clone PID

FXC00589

ORF Sequence

DNA sequence (1455 bp)
Protein sequence (485 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383937]

Gene

KIAA0646

Symbol

RNF8

Alias : hRNF8

Description

ring finger protein 8, E3 ubiquitin protein ligase, transcript variant 1

Original Clone ID

hj03808

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1455 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 485 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4834.1 1.4e-85 100.0 RNF8 CCDS4833.1 9.2e-72 100.0 RNF8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000253 38 109 PF00498 Forkhead-associated (FHA) domain IPR018957 403 440 PF00097 Zinc finger HMMSmart IPR000253 37 92 SM00240 Forkhead-associated (FHA) domain IPR001841 403 440 SM00184 Zinc finger ProfileScan IPR000253 38 92 PS50006 Forkhead-associated (FHA) domain IPR001841 403 441 PS50089 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003949 485 611685 100.0 100.0 XP_016866951 428 611685 100.0 88.2 NP_898901 448 611685 100.0 84.9 XP_006715304 455 611685 93.8 100.0 XP_006715305 418 611685 92.7 84.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0646 Download

>KIAA0646  1455 bp
ATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTG
CGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTA
GGACGAGGATTTGGTGTCACATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCT
CGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAG
AGTCTAAATGGTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATT
CATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAATGCGGAGTAT
GAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAAT
GACCAAATGATAGAAAAAAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAA
TTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCT
TGTGAATCTGGTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCT
GACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGGGGCTCCCATT
TACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCA
GCATCTCAGAGAAGCTTACAGATGTTTAAGGTGACCATGTCCAGGATTCTGAGGCTCAAA
ATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGG
AACTCAAAGAAAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGT
GCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAG
GAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTG
GCCCAGGCTCTGCAGGAGCATTGGGCTCTAATGGAAGAGCTAAATCGCAGCAAGAAGGAC
TTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAG
AAGATGCAAGCACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAG
CTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCAC
AGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGT
CGGAAGGACATTAAGTCCAAAACGTACTCTTTGGTTCTGGACAATTGCATTAATAAGATG
GTAAATAATCTGAGCTCAGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAA
GCAAAGAGATTGTTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0646 Download

>KIAA0646  485 aa
MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMIS
RNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEY
EYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVS
CESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSS
ASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLC
AEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKD
FEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAH
SFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERK
AKRLF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0646 Download

>pFN21ASDA0646  6184 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGGGGAGCCCGGCTT
CTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTGCGGCGGGTGGGGATGAG
CGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTAGGACGAGGATTTGGTGT
CACATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCTCGAAACCACTGTGTTTT
GAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAGAGTCTAAATGGTGTTTG
GCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATTCATCAGGGAGACTACAT
CCAACTTGGAGTGCCTCTGGAAAATAAGGAGAATGCGGAGTATGAATATGAAGTTACTGA
AGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAATGACCAAATGATAGAAAA
AAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAATTAGCAGGTCCTGGAGC
TGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCTTGTGAATCTGGTCAGCC
AGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCTGACAATTTGGATCCTAA
GTTGACTGCCCTTGAGCCAAGTAAGACCACAGGGGCTCCCATTTACCCTGGCTTCCCCAA
AGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCAGCATCTCAGAGAAGCTT
ACAGATGTTTAAGGTGACCATGTCCAGGATTCTGAGGCTCAAAATACAGATGCAGGAAAA
ACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGGAACTCAAAGAAAGTTGT
GCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGTGCAGAGCAGGCTCAGCA
GCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAGGAACAGCATCTTCAGGG
TTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTGGCCCAGGCTCTGCAGGA
GCATTGGGCTCTAATGGAAGAGCTAAATCGCAGCAAGAAGGACTTTGAAGCAATCATTCA
AGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAGAAGATGCAAGCACAGAA
GGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAGCTCCAATGTATTATTTG
TTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCACAGTTTCTGCTCCTACTG
TATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGTCGGAAGGACATTAAGTC
CAAAACGTACTCTTTGGTTCTGGACAATTGCATTAATAAGATGGTAAATAATCTGAGCTC
AGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAAGCAAAGAGATTGTTCTA
CGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT