(Circular) (Six-base) MAPSORT of: pFN21ASDA0646.seq Check: 4105 from: 1 to: 6184 pFN21ASDA0646 6184 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2541 2724 2882 2978 3050 4247 5631 Size: 786 123 183 158 96 72 1197 1384 Cuts at: 5631 5899 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1197 786 268 183 158 123 96 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 2908 3355 4913 2908 Size: 447 1558 4179 Fragments arranged by size: 4179 1558 447 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 2513 3052 3288 3927 5499 2513 Size: 539 236 639 1572 3198 Fragments arranged by size: 3198 1572 639 539 236 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2486 2533 3493 3719 3871 5980 1493 Size: 993 47 960 226 152 2109 1697 Fragments arranged by size: 2109 1697 993 960 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 3393 4738 160 Size: 872 2361 1345 1606 Fragments arranged by size: 2361 1606 1345 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 3509 1870 Size: 73 1566 4545 Fragments arranged by size: 4545 1566 73 BamHI G'GATC_C Cuts at: 2632 3514 3881 2632 Size: 882 367 4935 Fragments arranged by size: 4935 882 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2076 3505 4960 618 Size: 325 217 627 289 1429 1455 1842 Fragments arranged by size: 1842 1455 1429 627 325 289 217 BanII G_rGCy'C Cuts at: 1391 2034 2677 3070 3225 3419 3503 1391 Size: 643 643 393 155 194 84 4072 Fragments arranged by size: 4072 643 643 393 194 155 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 2972 928 Size: 971 510 563 4140 Fragments arranged by size: 4140 971 563 510 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 1548 Size: 6184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6179 6179 Size: 6184 BlpI GC'TnA_GC Cuts at: 2934 3601 2934 Size: 667 5517 Bme1580I G_kGCm'C Cuts at: 1790 3280 4245 5401 1790 Size: 1490 965 1156 2573 Fragments arranged by size: 2573 1490 1156 965 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2416 3915 4873 Size: 416 53 83 399 24 999 1499 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1753 1499 999 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2535 4844 1239 Size: 1296 2309 2579 Fragments arranged by size: 2579 2309 1296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 4309 5174 5472 2672 Size: 1637 865 298 3384 Fragments arranged by size: 3384 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 4847 882 Size: 617 547 2801 2219 Fragments arranged by size: 2801 2219 617 547 BsaAI yAC'GTr Cuts at: 493 1204 3481 3975 493 Size: 711 2277 494 2702 Fragments arranged by size: 2702 2277 711 494 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2659 2689 1178 Size: 30 165 30 1256 30 4673 Fragments arranged by size: 4673 1256 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6184 BseYI C'CCAG_C Cuts at: 2913 5387 5883 2913 Size: 2474 496 3214 Fragments arranged by size: 3214 2474 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2943 1163 Size: 573 1207 4404 Fragments arranged by size: 4404 1207 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3225 3419 4245 4330 5401 3225 Size: 194 826 85 1071 4008 Fragments arranged by size: 4008 1071 826 194 85 BsmI GAATG_Cn' Cuts at: 2377 3335 3695 3788 2377 Size: 958 360 93 4773 Fragments arranged by size: 4773 958 360 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2677 3070 3225 3280 3419 3503 Size: 399 244 643 393 155 55 139 84 Cuts at: 3503 4245 4330 5401 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 399 393 244 155 139 85 84 55 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 2716 4073 5803 2716 Size: 1357 1730 3097 Fragments arranged by size: 3097 1730 1357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 3539 844 Size: 1654 1041 3489 Fragments arranged by size: 3489 1654 1041 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4679 4853 65 Size: 1183 3431 174 1396 Fragments arranged by size: 3431 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 4828 5015 1700 Size: 110 250 2768 187 2869 Fragments arranged by size: 2869 2768 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 2007 4988 2007 Size: 2981 3203 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 4595 BstYI r'GATC_y Cuts at: 1972 2632 3514 3881 4266 4283 5735 5821 5833 Size: 660 882 367 385 17 1452 86 12 Cuts at: 5833 6179 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 882 660 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2232 2232 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4675 Fragments arranged by size: 4675 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5445 Fragments arranged by size: 5445 683 56 BtsI GCAGTG_nn' Cuts at: 3700 4494 4514 3700 Size: 794 20 5370 Fragments arranged by size: 5370 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2607 3631 Size: 54 1425 50 275 18 235 542 1024 Cuts at: 3631 4520 6149 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1024 889 542 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2743 2973 3072 3147 4114 1353 Size: 1390 230 99 75 967 3423 Fragments arranged by size: 3423 1390 967 230 99 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3223 3417 3223 Size: 194 5990 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2418 2535 2541 2724 2882 2978 3050 Size: 393 786 117 6 183 158 96 72 Cuts at: 3050 4247 4844 5631 5899 1239 Size: 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 787 786 597 393 268 183 158 117 96 72 6 EcoO109I rG'GnC_Cy Cuts at: 2510 2525 3025 2510 Size: 15 500 5669 Fragments arranged by size: 5669 500 15 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3118 3206 3118 Size: 88 6096 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2103 5331 1678 Size: 425 3228 2531 Fragments arranged by size: 3228 2531 425 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2142 2174 2185 2217 2322 Size: 32 135 32 570 32 11 32 105 Cuts at: 2322 2354 2404 2436 2894 2926 3341 3373 4825 Size: 32 50 32 458 32 415 32 1452 Cuts at: 4825 4857 4899 4931 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1452 570 458 415 135 105 50 42 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2643 3528 3774 669 Size: 988 281 705 885 246 3079 Fragments arranged by size: 3079 988 885 705 281 246 HindIII A'AGCT_T Cuts at: 748 2755 748 Size: 2007 4177 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3385 3783 3385 Size: 398 5786 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2365 3323 5298 5482 1305 Size: 1060 958 1975 184 2007 Fragments arranged by size: 2007 1975 1060 958 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2609 10 Size: 54 1425 343 777 3585 Fragments arranged by size: 3585 1425 777 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3201 4142 4501 4660 518 Size: 648 2035 941 359 159 2042 Fragments arranged by size: 2042 2035 941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2917 3040 3600 4277 5425 5670 1606 Size: 1311 123 560 677 1148 245 2120 Fragments arranged by size: 2120 1311 1148 677 560 245 123 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4675 Fragments arranged by size: 4675 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 3542 5087 1132 Size: 97 2313 1545 2229 Fragments arranged by size: 2313 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6184 PfoI T'CCnGG_A Cuts at: 2512 2783 2973 2512 Size: 271 190 5723 Fragments arranged by size: 5723 271 190 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2510 3025 2510 Size: 515 5669 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3223 3255 3223 Size: 32 6152 PstI C_TGCA'G Cuts at: 830 3057 3536 830 Size: 2227 479 3478 Fragments arranged by size: 3478 2227 479 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2917 3040 2917 Size: 123 6061 SacI G_AGCT'C Cuts at: 3225 3419 3225 Size: 194 5990 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SapI GCTCTTCn'nnn_ Cuts at: 3072 3072 Size: 6184 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2139 3053 3532 4667 5348 5539 826 Size: 219 1094 914 479 1135 681 191 1471 Fragments arranged by size: 1471 1135 1094 914 681 479 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 2060 1700 Size: 360 5824 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 4288 5189 5451 820 Size: 197 926 708 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 708 262 197 SnaBI TAC'GTA Cuts at: 493 3481 3975 493 Size: 2988 494 2702 Fragments arranged by size: 2988 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 3542 1132 Size: 2410 3774 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6184 StyI C'CwwG_G Cuts at: 513 1065 2022 3013 3147 3623 513 Size: 552 957 991 134 476 3074 Fragments arranged by size: 3074 991 957 552 476 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 4211 4228 4381 4566 5011 325 Size: 1255 535 2096 17 153 185 445 1498 Fragments arranged by size: 2096 1498 1255 535 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2612 4430 6142 Size: 274 80 33 51 1443 635 1818 1712 Cuts at: 6142 96 Size: 138 Fragments arranged by size: 1818 1712 1443 635 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2659 2691 6144 6176 2111 Size: 32 516 32 3453 32 2119 Fragments arranged by size: 3453 2119 516 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2779 1316 Size: 1463 4721 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 2969 3186 4313 2969 Size: 217 1127 4840 Fragments arranged by size: 4840 1127 217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI