KLHL8 ORF clone FHC00223

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00223

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Clone info.

Species

human

Clone Name

pFN21AA1378 Sequence (with vector) Restriction Map

Product ID

FHC00223

Flexi Clone PID

FXC00223

ORF Sequence

DNA sequence (1860 bp)
Protein sequence (620 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385460]

Gene

KIAA1378

Symbol

KLHL8

Description

kelch-like family member 8, transcript variant 2

Original Clone ID

fj04831s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1860 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 620 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3617.1 0 100.0 KLHL8 CCDS77937.1 1.1e-201 87.7 KLHL8 CCDS75163.1 2e-154 70.5 KLHL8 CCDS1310.1 1.1e-93 44.7 KLHL20 CCDS1429.1 4.6e-83 38.5 KLHL12 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 365 378 PR00501 NULL NULL 476 490 PR00501 NULL NULL 538 550 PR00501 NULL HMMPfam IPR013069 60 161 PF00651 BTB/POZ IPR011705 169 270 PF07707 BTB/Kelch-associated IPR006652 318 353 PF01344 Kelch repeat type 1 IPR006652 355 400 PF01344 Kelch repeat type 1 IPR006652 403 447 PF01344 Kelch repeat type 1 IPR006652 449 493 PF01344 Kelch repeat type 1 IPR006652 496 539 PF01344 Kelch repeat type 1 IPR006652 543 584 PF01344 Kelch repeat type 1 HMMSmart IPR000210 67 164 SM00225 BTB/POZ-like IPR011705 169 271 SM00875 BTB/Kelch-associated IPR006652 319 366 SM00612 Kelch repeat type 1 IPR006652 367 413 SM00612 Kelch repeat type 1 IPR006652 414 460 SM00612 Kelch repeat type 1 IPR006652 461 507 SM00612 Kelch repeat type 1 IPR006652 508 554 SM00612 Kelch repeat type 1 IPR006652 555 601 SM00612 Kelch repeat type 1 ProfileScan IPR000210 67 134 PS50097 BTB/POZ-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 76 KLISCHKLVLACVIPYFRAMFLS 98 PRIMARY 23 2 136 NVQPLLYAACILQVELVARACCE 158 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001278932 620 611967 100.0 100.0 NP_065854 620 611967 100.0 100.0 NP_001278935 544 611967 87.7 100.0 NP_001278936 437 611967 70.5 100.0 NP_067646 568 614522 38.5 89.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1378 Download

>KIAA1378  1860 bp
ATGGCTTCAGATTCTATGAGTAGTAAACAAGCTAGGAATCACATTACAAAGGGGAAAAGG
CAACAACAGCACCAGCAAATAAAGAACAGATCCTCAATTAGTGATGGTGATGGAGAAGAT
TCCTTTATTTTTGAAGCAAATGAAGCTTGGAAAGATTTTCATGGTTCTCTTCTTCGATTT
TATGAAAATGGAGAACTCTGTGATGTCACACTCAAGGTTGGCTCAAAGCTAATCTCTTGT
CACAAGCTGGTATTGGCTTGTGTTATTCCCTACTTTAGAGCCATGTTTCTTTCTGAAATG
GCTGAAGCCAAGCAAACGCTGATTGAGATTAGAGATTTTGATGGTGATGCAATAGAAGAC
TTGGTAAAGTTTGTCTATTCTTCACGGCTCACTTTGACTGTTGACAATGTCCAGCCTCTC
TTATATGCAGCCTGTATTCTGCAGGTTGAACTGGTGGCTAGAGCTTGTTGTGAATACATG
AAGTTACATTTTCATCCCTCCAATTGCCTGGCAGTAAGAGCCTTTGCAGAAAGTCACAAT
CGAATAGACTTAATGGACATGGCGGATCAGTATGCCTGTGACCATTTTACTGAAGTAGTG
GAGTGTGAAGACTTTGTAAGTGTATCACCGCAGCACCTCCATAAGCTTTTGTCCTCCAGT
GATCTAAATATTGAAAATGAAAAGCAGGTCTATAATGCTGCCATCAAGTGGCTTCTTGCC
AATCCTCAGCATCATTCCAAATGGTTGGATGAAACACTTGCACAGGTTCGCCTGCCATTG
TTGCCGGTTGATTTTCTTATGGGTGTTGTGGCAAAAGAACAGATTGTCAAGCAAAATCTA
AAATGTAGAGATTTACTGGATGAAGCAAGAAATTACCACCTTCACTTGAGTAGCAGAGCA
GTACCTGACTTTGAATACTCCATTCGGACTACCCCAAGGAAGCATACTGCTGGTGTGCTG
TTTTGTGTAGGTGGTCGAGGTGGATCTGGTGACCCCTTTCGCAGTATTGAATGCTATTCT
ATCAACAAAAACAGTTGGTTCTTTGGACCAGAAATGAATAGTCGAAGGCGACATGTGGGT
GTAATCTCTGTGGAAGGTAAAGTGTATGCAGTAGGTGGACATGATGGAAATGAACATTTA
GGGAGTATGGAGATGTTTGATCCTCTCACTAATAAATGGATGATGAAGGCATCAATGAAC
ACAAAGAGGCGAGGAATTGCCTTGGCTTCCTTAGGAGGCCCAATTTATGCAATTGGAGGG
TTAGATGACAATACTTGCTTCAATGATGTGGAGAGATATGACATAGAATCTGATCAGTGG
AGTACAGTGGCACCAATGAATACTCCCCGTGGAGGAGTTGGCTCTGTTGCTCTAGTAAAC
CATGTTTATGCAGTAGGTGGCAATGATGGAATGGCTTCTTTATCTAGCGTGGAGAGATAT
GATCCACATCTGGATAAGTGGATAGAAGTTAAAGAAATGGGTCAGCGAAGAGCAGGCAAT
GGAGTTAGCAAGCTTCATGGTTGCTTATACGTAGTTGGTGGTTTTGATGATAATTCTCCT
CTGAGTTCAGTTGAGCGGTATGACCCCCGAAGCAACAAGTGGGATTATGTGGCAGCACTT
ACTACTCCCAGAGGTGGAGTGGGAATCGCAACAGTGATGGGCAAAATCTTTGCAGTTGGT
GGTCATAATGGCAATGCATACTTAAATACAGTAGAAGCGTTTGATCCAGTGCTGAATAGG
TGGGAGCTTGTTGGATCTGTGTCTCACTGCAGAGCTGGAGCAGGAGTAGCTGTGTGTTCC
TGTTTAACTAGCCAAATTCGAGATGTAGGTCATGGATCCAATAATGTGGTTGACTGTATG

ORF Protein

Cloned ORF protein sequence for pFN21AA1378 Download

>KIAA1378  620 aa
MASDSMSSKQARNHITKGKRQQQHQQIKNRSSISDGDGEDSFIFEANEAWKDFHGSLLRF
YENGELCDVTLKVGSKLISCHKLVLACVIPYFRAMFLSEMAEAKQTLIEIRDFDGDAIED
LVKFVYSSRLTLTVDNVQPLLYAACILQVELVARACCEYMKLHFHPSNCLAVRAFAESHN
RIDLMDMADQYACDHFTEVVECEDFVSVSPQHLHKLLSSSDLNIENEKQVYNAAIKWLLA
NPQHHSKWLDETLAQVRLPLLPVDFLMGVVAKEQIVKQNLKCRDLLDEARNYHLHLSSRA
VPDFEYSIRTTPRKHTAGVLFCVGGRGGSGDPFRSIECYSINKNSWFFGPEMNSRRRHVG
VISVEGKVYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGG
LDDNTCFNDVERYDIESDQWSTVAPMNTPRGGVGSVALVNHVYAVGGNDGMASLSSVERY
DPHLDKWIEVKEMGQRRAGNGVSKLHGCLYVVGGFDDNSPLSSVERYDPRSNKWDYVAAL
TTPRGGVGIATVMGKIFAVGGHNGNAYLNTVEAFDPVLNRWELVGSVSHCRAGAGVAVCS
CLTSQIRDVGHGSNNVVDCM

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1378 Download

>pFN21AA1378  6565 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTCAGATTCTATGAGTAGTAAACAAGCTAG
GAATCACATTACAAAGGGGAAAAGGCAACAACAGCACCAGCAAATAAAGAACAGATCCTC
AATTAGTGATGGTGATGGAGAAGATTCCTTTATTTTTGAAGCAAATGAAGCTTGGAAAGA
TTTTCATGGTTCTCTTCTTCGATTTTATGAAAATGGAGAACTCTGTGATGTCACACTCAA
GGTTGGCTCAAAGCTAATCTCTTGTCACAAGCTGGTATTGGCTTGTGTTATTCCCTACTT
TAGAGCCATGTTTCTTTCTGAAATGGCTGAAGCCAAGCAAACGCTGATTGAGATTAGAGA
TTTTGATGGTGATGCAATAGAAGACTTGGTAAAGTTTGTCTATTCTTCACGGCTCACTTT
GACTGTTGACAATGTCCAGCCTCTCTTATATGCAGCCTGTATTCTGCAGGTTGAACTGGT
GGCTAGAGCTTGTTGTGAATACATGAAGTTACATTTTCATCCCTCCAATTGCCTGGCAGT
AAGAGCCTTTGCAGAAAGTCACAATCGAATAGACTTAATGGACATGGCGGATCAGTATGC
CTGTGACCATTTTACTGAAGTAGTGGAGTGTGAAGACTTTGTAAGTGTATCACCGCAGCA
CCTCCATAAGCTTTTGTCCTCCAGTGATCTAAATATTGAAAATGAAAAGCAGGTCTATAA
TGCTGCCATCAAGTGGCTTCTTGCCAATCCTCAGCATCATTCCAAATGGTTGGATGAAAC
ACTTGCACAGGTTCGCCTGCCATTGTTGCCGGTTGATTTTCTTATGGGTGTTGTGGCAAA
AGAACAGATTGTCAAGCAAAATCTAAAATGTAGAGATTTACTGGATGAAGCAAGAAATTA
CCACCTTCACTTGAGTAGCAGAGCAGTACCTGACTTTGAATACTCCATTCGGACTACCCC
AAGGAAGCATACTGCTGGTGTGCTGTTTTGTGTAGGTGGTCGAGGTGGATCTGGTGACCC
CTTTCGCAGTATTGAATGCTATTCTATCAACAAAAACAGTTGGTTCTTTGGACCAGAAAT
GAATAGTCGAAGGCGACATGTGGGTGTAATCTCTGTGGAAGGTAAAGTGTATGCAGTAGG
TGGACATGATGGAAATGAACATTTAGGGAGTATGGAGATGTTTGATCCTCTCACTAATAA
ATGGATGATGAAGGCATCAATGAACACAAAGAGGCGAGGAATTGCCTTGGCTTCCTTAGG
AGGCCCAATTTATGCAATTGGAGGGTTAGATGACAATACTTGCTTCAATGATGTGGAGAG
ATATGACATAGAATCTGATCAGTGGAGTACAGTGGCACCAATGAATACTCCCCGTGGAGG
AGTTGGCTCTGTTGCTCTAGTAAACCATGTTTATGCAGTAGGTGGCAATGATGGAATGGC
TTCTTTATCTAGCGTGGAGAGATATGATCCACATCTGGATAAGTGGATAGAAGTTAAAGA
AATGGGTCAGCGAAGAGCAGGCAATGGAGTTAGCAAGCTTCATGGTTGCTTATACGTAGT
TGGTGGTTTTGATGATAATTCTCCTCTGAGTTCAGTTGAGCGGTATGACCCCCGAAGCAA
CAAGTGGGATTATGTGGCAGCACTTACTACTCCCAGAGGTGGAGTGGGAATCGCAACAGT
GATGGGCAAAATCTTTGCAGTTGGTGGTCATAATGGCAATGCATACTTAAATACAGTAGA
AGCGTTTGATCCAGTGCTGAATAGGTGGGAGCTTGTTGGATCTGTGTCTCACTGCAGAGC
TGGAGCAGGAGTAGCTGTGTGTTCCTGTTTAACTAGCCAAATTCGAGATGTAGGTCATGG
ATCCAATAATGTGGTTGACTGTATGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT