RALGPS1 ORF clone FHC00057

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00057

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AA0351 Sequence (with vector) Restriction Map

Product ID

FHC00057

Flexi Clone PID

FXC00057

ORF Sequence

DNA sequence (1671 bp)
Protein sequence (557 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385313]

Gene

KIAA0351

Symbol

RALGPS1

Alias : RALGEF2, RALGPS1A

Description

Ral GEF with PH domain and SH3 binding motif 1, transcript variant 1

Original Clone ID

hg01609

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1671 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 557 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35143.1 7.7e-190 100.0 RALGPS1 CCDS65733.1 1.1e-118 64.0 RALGPS2 CCDS55346.1 8.8e-114 87.2 RALGPS1 CCDS55345.1 8.9e-114 88.7 RALGPS1 CCDS55344.1 1.1e-97 100.0 RALGPS1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001895 48 218 PF00617 Guanine-nucleotide dissociation stimulator CDC25 IPR001849 435 543 PF00169 Pleckstrin homology domain HMMSmart IPR001895 46 290 SM00147 Guanine-nucleotide dissociation stimulator CDC25 IPR001849 432 545 SM00233 Pleckstrin homology domain ProfileScan IPR001895 50 289 PS50009 Guanine-nucleotide dissociation stimulator CDC25 IPR001849 431 543 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016870836 557 614444 100.0 100.0 NP_055451 557 614444 100.0 100.0 XP_016870835 549 614444 98.6 100.0 NP_001309251 576 614444 94.0 100.0 XP_011517535 585 614444 95.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0351 Download

>KIAA0351  1671 bp
ATGTACAAGAGGAATGGTCTGATGGCTAGCGTGTTGGTCACCTCTGCCACTCCACAGGGC
AGCAGCAGCTCGGACTCTCTGGAGGGCCAGAGCTGCGACTATGCCAGCAAGAGCTATGAT
GCCGTTGTCTTCGATGTCTTGAAAGTGACCCCAGAGGAGTTTGCTAGCCAGATTACATTA
ATGGATATACCTGTGTTTAAAGCTATCCAGCCGGAGGAACTAGCCAGCTGTGGATGGAGT
AAGAAGGAGAAACACAGTCTTGCCCCTAACGTTGTGGCCTTTACCCGGAGGTTTAACCAG
GTCAGTTTTTGGGTTGTACGAGAAATTCTAACAGCACAGACTTTAAAAATAAGGGCAGAA
ATCCTCAGCCATTTTGTGAAAATAGCCAAGAAACTTCTAGAACTCAACAACCTTCATTCT
CTCATGTCTGTGGTATCAGCATTACAAAGTGCTCCCATCTTCAGGCTGACAAAAACCTGG
GCTCTTTTAAATCGAAAAGACAAGACTACCTTTGAGAAATTGGACTACCTGATGTCGAAA
GAAGATAATTACAAGCGGACACGGGAATATATCCGAAGCCTGAAGATGGTTCCAAGTATT
CCCTATCTAGGAATCTATCTTCTGGATTTAATCTACATTGATTCTGCATATCCTGCCTCA
GGCAGTATCATGGAAAATGAACAAAGATCCAATCAGATGAACAATATTCTTCGAATAATT
GCTGATTTACAAGTTTCCTGCAGCTATGATCACCTCACCACCCTGCCCCATGTGCAGAAG
TACCTGAAGTCCGTACGCTACATTGAAGAGCTCCAGAAGTTTGTGGAAGACGACAACTAC
AAACTGTCGCTCAGAATCGAACCAGGAAGCAGCTCTCCAAGACTAGTCTCTTCCAAGGAA
GATCTTGCAGGTCCCTCTGCTGGCTCCGGTTCTGCGAGGTTCAGCCGGAGGCCCACCTGT
CCTGACACATCTGTTGCTGGCAGCCTCCCCACACCTCCAGTCCCCAGACACAGGAAGAGC
CACAGCCTAGGCAACAATATGATGTGTCAGTTGAGTGTAGTTGAGAGTAAAAGTGCGACA
TTCCCATCGGAGAAAGCAAGGCACCTACTGGACGACAGTGTCCTAGAGTCCCGCAGCCCC
CGAAGGGGCCTGGCTCTGACCTCCTCCTCTGCTGTCACCAATGGACTCTCCCTAGGCAGT
AGTGAGAGCTCAGAGTTTAGTGAAGAGATGTCTTCAGGGCTGGAAAGCCCCACCGGCCCG
TGCATCTGTTCTCTGGGGAACTCCGCAGCTGTGCCCACCATGGAGGGGCCTCTGAGAAGA
AAAACCCTGCTCAAGGAAGGGCGGAAGCCTGCGCTGTCCTCGTGGACCAGGTACTGGGTC
ATACTCTCAGGATCCACCCTCCTGTACTACGGAGCCAAGTCCTTGCGGGGCACAGACAGA
AAACACTATAAATCCACACCTGGCAAAAAGGTTTCCATCGTGGGCTGGATGGTGCAGCTG
CCCGATGACCCCGAGCACCCAGATATCTTCCAGCTGAACAACCCTGACAAAGGCAATGTT
TACAAGTTTCAGACTGGTTCCCGATTTCATGCAATACTGTGGCACAAGCATTTGGATGAT
GCATGTAAAAGCAACAGGCCTCAGGTACCTGCAAACCTTATGTCATTTGAG

ORF Protein

Cloned ORF protein sequence for pFN21AA0351 Download

>KIAA0351  557 aa
MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVFDVLKVTPEEFASQITL
MDIPVFKAIQPEELASCGWSKKEKHSLAPNVVAFTRRFNQVSFWVVREILTAQTLKIRAE
ILSHFVKIAKKLLELNNLHSLMSVVSALQSAPIFRLTKTWALLNRKDKTTFEKLDYLMSK
EDNYKRTREYIRSLKMVPSIPYLGIYLLDLIYIDSAYPASGSIMENEQRSNQMNNILRII
ADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVEDDNYKLSLRIEPGSSSPRLVSSKE
DLAGPSAGSGSARFSRRPTCPDTSVAGSLPTPPVPRHRKSHSLGNNMMCQLSVVESKSAT
FPSEKARHLLDDSVLESRSPRRGLALTSSSAVTNGLSLGSSESSEFSEEMSSGLESPTGP
CICSLGNSAAVPTMEGPLRRKTLLKEGRKPALSSWTRYWVILSGSTLLYYGAKSLRGTDR
KHYKSTPGKKVSIVGWMVQLPDDPEHPDIFQLNNPDKGNVYKFQTGSRFHAILWHKHLDD
ACKSNRPQVPANLMSFE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0351 Download

>pFN21AA0351  6376 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTACAAGAGGAATGGTCTGATGGCTAGCGTGTT
GGTCACCTCTGCCACTCCACAGGGCAGCAGCAGCTCGGACTCTCTGGAGGGCCAGAGCTG
CGACTATGCCAGCAAGAGCTATGATGCCGTTGTCTTCGATGTCTTGAAAGTGACCCCAGA
GGAGTTTGCTAGCCAGATTACATTAATGGATATACCTGTGTTTAAAGCTATCCAGCCGGA
GGAACTAGCCAGCTGTGGATGGAGTAAGAAGGAGAAACACAGTCTTGCCCCTAACGTTGT
GGCCTTTACCCGGAGGTTTAACCAGGTCAGTTTTTGGGTTGTACGAGAAATTCTAACAGC
ACAGACTTTAAAAATAAGGGCAGAAATCCTCAGCCATTTTGTGAAAATAGCCAAGAAACT
TCTAGAACTCAACAACCTTCATTCTCTCATGTCTGTGGTATCAGCATTACAAAGTGCTCC
CATCTTCAGGCTGACAAAAACCTGGGCTCTTTTAAATCGAAAAGACAAGACTACCTTTGA
GAAATTGGACTACCTGATGTCGAAAGAAGATAATTACAAGCGGACACGGGAATATATCCG
AAGCCTGAAGATGGTTCCAAGTATTCCCTATCTAGGAATCTATCTTCTGGATTTAATCTA
CATTGATTCTGCATATCCTGCCTCAGGCAGTATCATGGAAAATGAACAAAGATCCAATCA
GATGAACAATATTCTTCGAATAATTGCTGATTTACAAGTTTCCTGCAGCTATGATCACCT
CACCACCCTGCCCCATGTGCAGAAGTACCTGAAGTCCGTACGCTACATTGAAGAGCTCCA
GAAGTTTGTGGAAGACGACAACTACAAACTGTCGCTCAGAATCGAACCAGGAAGCAGCTC
TCCAAGACTAGTCTCTTCCAAGGAAGATCTTGCAGGTCCCTCTGCTGGCTCCGGTTCTGC
GAGGTTCAGCCGGAGGCCCACCTGTCCTGACACATCTGTTGCTGGCAGCCTCCCCACACC
TCCAGTCCCCAGACACAGGAAGAGCCACAGCCTAGGCAACAATATGATGTGTCAGTTGAG
TGTAGTTGAGAGTAAAAGTGCGACATTCCCATCGGAGAAAGCAAGGCACCTACTGGACGA
CAGTGTCCTAGAGTCCCGCAGCCCCCGAAGGGGCCTGGCTCTGACCTCCTCCTCTGCTGT
CACCAATGGACTCTCCCTAGGCAGTAGTGAGAGCTCAGAGTTTAGTGAAGAGATGTCTTC
AGGGCTGGAAAGCCCCACCGGCCCGTGCATCTGTTCTCTGGGGAACTCCGCAGCTGTGCC
CACCATGGAGGGGCCTCTGAGAAGAAAAACCCTGCTCAAGGAAGGGCGGAAGCCTGCGCT
GTCCTCGTGGACCAGGTACTGGGTCATACTCTCAGGATCCACCCTCCTGTACTACGGAGC
CAAGTCCTTGCGGGGCACAGACAGAAAACACTATAAATCCACACCTGGCAAAAAGGTTTC
CATCGTGGGCTGGATGGTGCAGCTGCCCGATGACCCCGAGCACCCAGATATCTTCCAGCT
GAACAACCCTGACAAAGGCAATGTTTACAAGTTTCAGACTGGTTCCCGATTTCATGCAAT
ACTGTGGCACAAGCATTTGGATGATGCATGTAAAAGCAACAGGCCTCAGGTACCTGCAAA
CCTTATGTCATTTGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT