(Circular) (Six-base) MAPSORT of: pFN21AA0351.seq Check: 9331 from: 1 to: 6376 pFN21AA0351 6376 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3719 1937 Size: 1782 4594 Acc65I G'GTAC_C Cuts at: 3649 3697 3649 Size: 48 6328 AclI AA'CG_TT Cuts at: 1563 2274 4503 4876 1563 Size: 711 2229 373 3063 Fragments arranged by size: 3063 2229 711 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2449 2606 2810 3222 4439 5823 6091 1632 Size: 817 157 204 412 1217 1384 268 1917 Fragments arranged by size: 1917 1384 1217 817 412 268 204 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 3378 4119 5691 3378 Size: 741 1572 4063 Fragments arranged by size: 4063 1572 741 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2328 3685 3911 4063 6172 1493 Size: 835 1357 226 152 2109 1697 Fragments arranged by size: 2109 1697 1357 835 226 152 AseI AT'TA_AT Cuts at: 160 1032 2183 4930 160 Size: 872 1151 2747 1606 Fragments arranged by size: 2747 1606 1151 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3515 3701 1870 Size: 73 1572 186 4545 Fragments arranged by size: 4545 1572 186 73 AvrII C'CTAG_G Cuts at: 3031 3196 3031 Size: 165 6211 BamHI G'GATC_C Cuts at: 3395 3706 4073 3395 Size: 311 367 5698 Fragments arranged by size: 5698 367 311 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3105 3649 3697 5152 618 Size: 325 217 627 1318 544 48 1455 1842 Fragments arranged by size: 1842 1455 1318 627 544 325 217 48 BanII G_rGCy'C Cuts at: 1391 2488 2817 3215 3695 1391 Size: 1097 329 398 480 4072 Fragments arranged by size: 4072 1097 480 398 329 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2125 2838 3228 928 Size: 971 226 713 390 4076 Fragments arranged by size: 4076 971 713 390 226 BbvCI CC'TCA_GC Cuts at: 2369 2369 Size: 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 2752 1548 Size: 1204 5172 BfrBI ATG'CAT Cuts at: 3626 3626 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3258 4987 136 Size: 107 122 71 631 605 1586 1729 1525 Fragments arranged by size: 1729 1586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2905 6371 2905 Size: 3466 2910 BlpI GC'TnA_GC Cuts at: 3793 3793 Size: 6376 Bme1580I G_kGCm'C Cuts at: 1790 3300 3437 4437 5593 1790 Size: 1510 137 1000 1156 2573 Fragments arranged by size: 2573 1510 1156 1000 137 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3388 4107 5065 Size: 416 53 83 399 24 1971 719 958 Cuts at: 5065 442 Size: 1753 Fragments arranged by size: 1971 1753 958 719 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2034 2172 1056 Size: 978 138 5260 Fragments arranged by size: 5260 978 138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2801 2985 5036 1239 Size: 866 696 184 2051 2579 Fragments arranged by size: 2579 2051 866 696 184 Bpu10I CC'TnA_GC Cuts at: 2369 2369 Size: 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3320 4501 5366 5664 3320 Size: 1181 865 298 4032 Fragments arranged by size: 4032 1181 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2930 4809 5207 5481 5628 6039 2930 Size: 1879 398 274 147 411 3267 Fragments arranged by size: 3267 1879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6151 Fragments arranged by size: 6151 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 3157 3160 1186 Size: 988 983 3 4402 Fragments arranged by size: 4402 988 983 3 BseYI C'CCAG_C Cuts at: 5579 6075 5579 Size: 496 5880 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 3518 1163 Size: 573 1062 720 4021 Fragments arranged by size: 4021 1062 720 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2458 2817 3215 3522 4437 4522 5593 2458 Size: 359 398 307 915 85 1071 3241 Fragments arranged by size: 3241 1071 915 398 359 307 85 BsiWI C'GTAC_G Cuts at: 2797 2797 Size: 6376 BsmI GAATG_Cn' Cuts at: 3887 3980 3887 Size: 93 6283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2458 2488 2817 3215 3300 3437 3522 Size: 399 668 30 329 398 85 137 85 Cuts at: 3522 3695 4437 4522 5593 1391 Size: 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 399 398 329 173 137 85 85 85 30 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 3661 3731 844 Size: 2059 758 70 3489 Fragments arranged by size: 3489 2059 758 70 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3565 4871 5045 65 Size: 1183 2317 1306 174 1396 Fragments arranged by size: 2317 1396 1306 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3257 5020 5207 1700 Size: 110 1447 1763 187 2869 Fragments arranged by size: 2869 1763 1447 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 4465 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 3364 4436 5448 3364 Size: 1072 1012 4292 Fragments arranged by size: 4292 1072 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2657 794 Size: 1863 4513 BstBI TT'CG_AA Cuts at: 2716 5180 2716 Size: 2464 3912 BstEII G'GTnAC_C Cuts at: 1184 2041 1184 Size: 857 5519 BstYI r'GATC_y Cuts at: 1972 2690 2905 3395 3706 4073 4458 4475 5927 Size: 718 215 490 311 367 385 17 1452 Cuts at: 5927 6013 6025 6371 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 718 490 385 367 346 311 215 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2662 3645 2662 Size: 983 5393 BtgI C'CryG_G Cuts at: 513 1065 3303 513 Size: 552 2238 3586 Fragments arranged by size: 3586 2238 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 3892 4686 4706 3892 Size: 794 20 5562 Fragments arranged by size: 5562 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 2203 2349 2493 3680 2203 Size: 146 144 1187 4899 Fragments arranged by size: 4899 1187 146 144 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3122 5383 809 Size: 507 1806 2261 1802 Fragments arranged by size: 2261 1806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341 Size: 54 1425 50 275 18 1993 889 1629 Cuts at: 6341 8 Size: 43 Fragments arranged by size: 1993 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 2899 3014 3222 4306 1353 Size: 1452 94 115 208 1084 3423 Fragments arranged by size: 3423 1452 1084 208 115 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3361 4963 5337 5483 387 Size: 1064 138 1772 1602 374 146 1280 Fragments arranged by size: 1772 1602 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2815 3213 2815 Size: 398 5978 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2449 2606 2801 2810 2985 3222 Size: 393 473 344 157 195 9 175 237 Cuts at: 3222 4439 5036 5823 6091 1239 Size: 1217 597 787 268 1524 Fragments arranged by size: 1524 1217 787 597 473 393 344 268 237 195 175 157 9 EcoNI CCTnn'n_nnAGG Cuts at: 2661 3335 3552 2661 Size: 674 217 5485 Fragments arranged by size: 5485 674 217 EcoO109I rG'GnC_Cy Cuts at: 2915 3151 3311 2915 Size: 236 160 5980 Fragments arranged by size: 5980 236 160 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 EcoRV GAT'ATC Cuts at: 3529 3529 Size: 6376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 6376 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 5523 1678 Size: 3845 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2005 2037 3387 3419 5017 Size: 32 135 32 433 32 1350 32 1598 Cuts at: 5017 5049 5091 5123 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1598 1350 433 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3720 3966 669 Size: 988 281 1782 246 3079 Fragments arranged by size: 3079 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3653 3701 3653 Size: 48 6328 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5490 5674 1305 Size: 4185 184 2007 Fragments arranged by size: 4185 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4554 Fragments arranged by size: 4554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 3618 4334 4693 4852 518 Size: 648 1266 1186 716 359 159 2042 Fragments arranged by size: 2042 1266 1186 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2232 3293 3502 3538 3792 4469 5617 5862 Size: 626 1061 209 36 254 677 1148 245 Cuts at: 5862 1606 Size: 2120 Fragments arranged by size: 2120 1148 1061 677 626 254 245 209 36 NcoI C'CATG_G Cuts at: 513 1065 3303 513 Size: 552 2238 3586 Fragments arranged by size: 3586 2238 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 2030 2168 1052 Size: 978 138 5260 Fragments arranged by size: 5260 978 138 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NsiI A_TGCA'T Cuts at: 3628 3628 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3630 3734 5279 1132 Size: 97 2401 104 1545 2229 Fragments arranged by size: 2401 2229 1545 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 3378 1108 Size: 2270 4106 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2915 2915 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PstI C_TGCA'G Cuts at: 830 2747 3728 830 Size: 1917 981 3478 Fragments arranged by size: 3478 1917 981 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2232 3293 3502 3538 2232 Size: 1061 209 36 5070 Fragments arranged by size: 5070 1061 209 36 SacI G_AGCT'C Cuts at: 2817 3215 2817 Size: 398 5978 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SapI GCTCTTCn'nnn_ Cuts at: 2805 3014 2805 Size: 209 6167 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SexAI A'CCwGG_T Cuts at: 2301 3371 2301 Size: 1070 5306 SfcI C'TryA_G Cuts at: 826 1045 2743 3724 4859 5540 5731 826 Size: 219 1698 981 1135 681 191 1471 Fragments arranged by size: 1698 1471 1135 981 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3335 4480 5381 5643 820 Size: 197 926 1392 1145 901 262 1553 Fragments arranged by size: 1553 1392 1145 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 2887 152 Size: 2735 3641 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 2710 4300 5 Size: 47 2658 1590 2081 Fragments arranged by size: 2658 2081 1590 47 StuI AGG'CCT Cuts at: 1517 3643 1517 Size: 2126 4250 StyI C'CwwG_G Cuts at: 513 1065 2898 3031 3196 3303 3815 513 Size: 552 1833 133 165 107 512 3074 Fragments arranged by size: 3074 1833 552 512 165 133 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3537 4403 4420 4573 4758 5203 325 Size: 1255 1957 866 17 153 185 445 1498 Fragments arranged by size: 1957 1498 1255 866 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3408 4622 Size: 274 80 33 51 1443 30 1401 1214 Cuts at: 4622 6334 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1401 1214 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 3440 3472 6336 6368 2988 Size: 32 420 32 2864 32 2996 Fragments arranged by size: 2996 2864 420 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3122 1316 Size: 1806 4570 XbaI T'CTAG_A Cuts at: 2401 3712 2401 Size: 1311 5065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BplI BsmBI BstXI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI