ARHGAP25 ORF clone FHC00387

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00387

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21ASDA0053 Sequence (with vector) Restriction Map

Product ID

FHC00387

Flexi Clone PID

FXC00387

ORF Sequence

DNA sequence (1914 bp)
Protein sequence (638 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383728]

Gene

KIAA0053

Symbol

ARHGAP25

Alias : HEL-S-308, KAIA0053

Description

Rho GTPase activating protein 25, transcript variant 2

Original Clone ID

ha01417

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1914 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 638 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46312.1 3.9e-153 100.0 ARHGAP25 CCDS54364.1 1.1e-152 99.8 ARHGAP25 CCDS33214.2 9.1e-150 99.8 ARHGAP25 CCDS54363.1 4.1e-115 93.8 ARHGAP25 CCDS7227.1 5.3e-45 42.9 ARHGAP22 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 41 142 PF00169 Pleckstrin homology domain IPR000198 170 318 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001849 40 146 SM00233 Pleckstrin homology domain IPR000198 167 343 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001849 39 144 PS50003 Pleckstrin homology domain IPR000198 152 346 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055697 638 610587 100.0 100.0 NP_001159748 639 610587 99.8 100.0 XP_016860913 649 610587 98.7 99.4 XP_005264732 645 610587 100.0 98.1 XP_016860912 650 610587 98.6 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0053 Download

>KIAA0053  1914 bp
ATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATG
ACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCC
ATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTAC
TTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAG
GGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCT
GGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCATGGGACAGGAC
TCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGG
AGAGTTGCTGGCACACCCTGTGGAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTAT
GAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTCATC
CTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTG
AAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACAGAT
GTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTT
CCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCA
AAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTC
CTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATG
AGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGAC
CCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGA
GACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAGAAA
AATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGAC
CTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGC
CCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAA
AAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAA
TGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAAT
GAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCT
CAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCTTCC
CTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAG
GGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAA
GAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAG
AAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCT
AAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAG
ATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTG
GAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0053 Download

>KIAA0053  638 aa
MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRY
FVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQD
SYVLMASSQAEMEEWVKFLRRVAGTPCGVFGQRLDETVAYEQKFGPHLVPILVEKCAEFI
LEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVV
PWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKM
SVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIPLSPPAQK
NDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPRE
KPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMS
QDLRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGE
EEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALE
ISLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTEA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0053 Download

>pFN21ASDA0053  6643 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGTCCCTCGGTCAGTC
GGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATGACTGGCGAGCAGATGGC
TGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCCATCAAGATGGGCTGGCT
GAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTGCTGAGGGCGCA
GCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTACC
AGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGA
AATCATTCCAGCCTCATGGGACCAGAATCGCATGGGACAGGACTCCTATGTCCTCATGGC
CAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGGAGAGTTGCTGGCACACC
CTGTGGAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTATGAACAGAAATTCGGCCC
CCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTCATCCTGGAGCACGGCCGGAA
TGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTGAAGCAGCTGAGAGACGC
TTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACAGATGTGCACACTGTGGCTTC
CCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTTCCCTGGAGCCAGTACGA
AGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCTCAGCAGGAGTT
GATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGCAG
GTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATGAGTGTGGACAACCTGGC
TACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGACCCTGCCGTGATCATGAG
AGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGAGACCATGAAGTCCTCTT
CCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAGAAAAATGACCCCAAGAAAGC
TCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGACCTCCGAATTTCTAGGAC
AGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGCCCCACCGGACAGCAGCC
GAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAAAAGCCAGGAGACTGGAA
AATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAATGTTTCTTGACATCAGC
TTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAATGAATTCTGGTCGCCTTC
CTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGACTTGCGCCAACT
TTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTGG
GGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAGGGAGATACTCTTGCCAG
TCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAAGAGGAAATTGATTCTTT
GCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAGAAGCAAATGTATGAGGA
ACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCTAAAGTGGTGAGGCTCAA
TGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAGATCAGCCTCCGCAACAT
GGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAAGAAGTCAAGGA
ATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCTTACGTAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT