HUGE

Gene/Protein Characteristic Table for KIAA0018

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00004
Accession No. :	D13643
Description :	24-dehydrocholesterol reductase precursor.
HUGO Gene Name :	24-dehydrocholesterol reductase (DHCR24)
Clone Name :	hk07284 [Vector Info]
Flexi ORF Clone :	pF1KA0018
Source :	Human adult brain
Note :	We replaced ha00517, former representative clones for KIAA0018 with hk07284. (2001/10/06)

Features of the cloned DNA sequence	Description

Length: 4268 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 2605 bp

Genome contig ID gi89161185r_54987889
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
TGCTTCTGTTTAAATAAAAGTGGCCTGGAAGCTGG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
CGTTTGTCGTTTCTTGCTTGGGGGACCAAAATGTGGAGTCAGATCTGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 55087889 55125493 9 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 553 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001108186	98.2	similar to 24-d...	Macaca mulatta
Q15392	100.0	24-dehydrochole...	Homo sapiens
AAP36155	100.0	24-dehydrochole...	synthetic construct
XP_001153873	99.8	24-dehydrochole...	Pan troglodytes
Q60HC5	99.4	24-dehydrochole...	Macaca fascicularis

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR006094 151 240 PF01565 FAD linked oxidase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006094	151	240	PF01565	FAD linked oxidase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 40 PAVSLAVCALLFLLWVRLKGLEF 62 PRIMARY 23

2 74 LFLLPLSLIFDIYYYVRAWVVFK 96 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	40	PAVSLAVCALLFLLWVRLKGLEF	62	PRIMARY	23
2	74	LFLLPLSLIFDIYYYVRAWVVFK	96	PRIMARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: TTGGCCAGAAGGATGAATAC
Primer_r	: AGTGCCTGTGGCTGTAAGAG
PCR product length	: 156 bp
PCR conditions	: 95 °C15 sec58 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	YES

Length of 3'UTR	2605 bp
Genome contig ID	gi89161185r_54987889
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- TGCTTCTGTTTAAATAAAAGTGGCCTGGAAGCTGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* CGTTTGTCGTTTCTTGCTTGGGGGACCAAAATGTGGAGTCAGATCTGGGC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	55087889	55125493	9	99.2	Perfect prediction

Gene/Protein Characteristic Table for KIAA0018

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information