Gene/Protein Characteristic Table for KIAA0882
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Product ID ORK01130
Accession No AB020689
Description TBC1 domain family, member 9 (with GRAM domain)
Clone name hk07544s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5286 bp)
Predicted protein sequence (1291 aa)
Flexi ORF Clone FXC01130
Source Human adult brain
Rouge ID mKIAA0882 by Kazusa Mouse cDNA Project
Note We replaced hk07544, former representative clones for KIAA0882 with hk07544s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5286 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1410 bp
Genome contig ID gi89161207r_141661389
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
GCTAAATGTGAACAAATAAAATACAGCAAATACTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGATACAGCTTTTTATCTTTGTGCTTGAGTTCCTGCCTAAGGCAATAACA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 4 r 141761389 141896724 21 99.6 Perfect prediction
Features of the protein sequence
Description

Length: 1291 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q6ZT07 0 100.0 TBC1 domain fam...
Homo sapiens
XP_533283 0 97.5 similar to Y45F...
Canis lupus fam...
XP_001090502 0 100.0 similar to Y45F...
Macaca mulatta
Q3UYK3 0 95.9 TBC1 domain fam...
Mus musculus
BAE22209 0 95.7 unnamed protein...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB014576 0 62.9 KIAA0676
AB028978 1.4e-21 33.6 KIAA1055
AB029031 1.9e-14 27.4 KIAA1108
D13644 1.6e-12 27.6 KIAA0019
AB011175 2.2e-12 26.5 KIAA0603
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR004182 171 238 PF02893 GRAM
IPR004182 318 386 PF02893 GRAM
IPR000195 537 749 PF00566 RabGAP/TBC
IPR002048 915 943 PF00036 Calcium-binding EF-hand
HMMSmart IPR004182 171 238 SM00568 GRAM
IPR004182 318 386 SM00568 GRAM
IPR000195 537 750 SM00164 RabGAP/TBC
ProfileScan IPR000195 540 727 PS50086 RabGAP/TBC
IPR002048 911 946 PS50222 Calcium-binding EF-hand

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 696 STISLSWFLTLFLSVMPFESAVV 718 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AAGTAGCAGCAAAGACAGAGG
Primer_r ATCCAGTATCAGAAGCATCAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 4
Experimental conditions
Panel name GeneBridge 4
Primer_f AAGTAGCAGCAAAGACAGAGG
Primer_r ATCCAGTATCAGAAGCATCAG
PCR product length 97 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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