Gene/Protein Characteristic Table for KIAA0727
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK01115
Accession No AB018270
Description myosin ID, transcript variant 1
Clone name hk03490y1
Vector information
The cDNA fragment was originally inserted at BssH II-NotI si ...
cDNA sequence DNA sequence (5182 bp)
Predicted protein sequence (1010 aa)
Flexi ORF Clone FXC01115
Source Human adult brain
Note We replaced hk03490, former representative clones for KIAA0727 with hk03490y1. (2002/12/27)
Features of the cloned cDNA sequence
Description

Length: 5182 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2149 bp
Genome contig ID gi51511734r_27743741
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
GATTTGCCATCAAGAGCAATAAAGCATTAAATCTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTTTTAAAGGTGTTCTGCTTATCTGTCACTGAAAACTTGGTGAAATTTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 17 r 27843741 28228015 22 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1010 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW80221 0 100.0 myosin ID, isof...
Homo sapiens
XP_511404 0 99.6 myosin ID [Pan ...
Pan troglodytes
O94832 0 100.0 Myosin-Id.
Homo sapiens
XP_548273 0 98.4 similar to Myos...
Canis lupus fam...
Q63357 0 98.0 Myosin-Id; Myos...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023217 5.4e-81 33.6 KIAA1000
AB020673 1e-74 35.8 KIAA0866
AB040945 9e-70 32.3 KIAA1512
AB032945 6.1e-69 34.5 KIAA1119
AB018342 4e-58 35.2 KIAA0799
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001609 142 169 PD000355 Myosin head
FPrintScan IPR001609 43 62 PR00193 Myosin head
IPR001609 99 124 PR00193 Myosin head
IPR001609 147 174 PR00193 Myosin head
IPR001609 382 410 PR00193 Myosin head
IPR001609 435 463 PR00193 Myosin head
HMMPfam IPR001609 17 686 PF00063 Myosin head
IPR000048 703 723 PF00612 IQ calmodulin-binding region
IPR000048 725 745 PF00612 IQ calmodulin-binding region
IPR010926 806 1009 PF06017 Myosin tail 2
HMMSmart IPR001609 7 700 SM00242 Myosin head
IPR000048 701 723 SM00015 IQ calmodulin-binding region
ProfileScan IPR000048 702 730 PS50096 IQ calmodulin-binding region
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGTGGCAACTGCAAAAGGATC
Primer_r GTCTACAATCATGCCCGCTTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 17
Experimental conditions
Panel name GeneBridge 4
Primer_f TGTGGCAACTGCAAAAGGATC
Primer_r GTCTACAATCATGCCCGCTTC
PCR product length 93 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp