Gene/Protein Characteristic Table for KIAA0712
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Product ID ORK00610
Accession No AB018255
Description Rho GTPase activating protein 32, transcript variant 2
Clone name hg04169
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6477 bp)
Predicted protein sequence (1770 aa)
Flexi ORF Clone FXC00610
Source Human adult brain
Rouge ID mKIAA0712 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6477 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 960 bp
Genome contig ID gi51511727r_128243052
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CATTGGTTTTCCTAAATTATCAACTTTCTTTCATT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAAAAAAAGCCCAGCATGGTTTGACTGGATAGACAC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 r 128343052 128399219 13 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1770 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW67738 0 100.0 Rho GTPase-acti...
Homo sapiens
A7KAX9 0 100.0 Rho/Cdc42/Rac G...
Homo sapiens
AAO43677 0 100.0 rac GTPase acti...
Homo sapiens
XP_546401 0 87.5 similar to Rho ...
Canis lupus fam...
XP_001056872 0 86.6 similar to Rho ...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB033030 3.2e-31 28.6 KIAA1204
AB014572 3.7e-07 24.2 KIAA0672
AB014521 1.5e-06 27.0 KIAA0621
D29642 1.2e-05 26.1 KIAA0053
AB040934 1.4e-05 30.0 KIAA1501
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000198 69 222 PF00620 RhoGAP
HMMSmart IPR000198 66 247 SM00324 RhoGAP
ProfileScan IPR000198 55 250 PS50238 RhoGAP
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGTGTTCAGTTAGAGTCGTGG
Primer_r CTGAGTCTAAGTGGTCTGGTG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f TGTGTTCAGTTAGAGTCGTGG
Primer_r CTGAGTCTAAGTGGTCTGGTG
PCR product length 146 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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