ROUGE

Gene/Protein Characteristic Table for mKIAA0199

Link to : HUGE \| SWISS-PROT/TrEMBL
Features : DNA sequence \| Protein sequence

Accession No. :	AK172912
Description :	SREBP cleavage activating protein. Sreb cleavage-activating protein.
Clone Name :	mfj09054 [Vector Info]
Source :	Mouse fetal brain

Features of the cloned DNA sequence	Description

Length: 4982 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 144 bp

Genome contig ID gi65519420f_110273398
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
CCTGACTGTAATAATAAACTTTTTTAAAAAACCAC
Flanking genome sequence
(153191 - 153240)
----+----*----+----*----+----*----+----*----+----*
ATTTACCAGGGGCTGAAGTTCTTGTCATTCCACACTCCTCCTAGACCCTT

Genomic structure deduced by Blast search against draft genome sequences
Comparison with the corresponding KIAA cDNA.

KIAA Alignment based on:

KIAA0199 DNA sequence, AA sequence, Physical map

KIAA	Alignment based on:
KIAA0199	DNA sequence, AA sequence, Physical map

Features of the protein sequence	Description

Coding region: 133..876, 1381..2580, 2949..4838
Length: 1277 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EDL09018	99.9	SREBP cleavage ...	Mus musculus
Q6GQT6	99.9	Sterol regulato...	Mus musculus
AAH70437	99.8	SREBF chaperone...	Mus musculus
A2RRU4	98.0	Sterol regulato...	Rattus norvegicus
P97260	96.2	Sterol regulato...	Cricetulus griseus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other ROUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	ROUGE_ID
BC064045	2.9e-05	27.0	mKIAA4167

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR001680 1090 1104 PR00320 WD-40 repeat

IPR001680 1131 1145 PR00320 WD-40 repeat

IPR001680 1171 1185 PR00320 WD-40 repeat

HMMPfam IPR001680 768 803 PF00400 WD-40 repeat

IPR001680 1068 1103 PF00400 WD-40 repeat

IPR001680 1108 1144 PF00400 WD-40 repeat

IPR001680 1149 1184 PF00400 WD-40 repeat

HMMSmart IPR001680 766 803 SM00320 WD-40 repeat

IPR001680 954 991 SM00320 WD-40 repeat

IPR001680 1063 1103 SM00320 WD-40 repeat

IPR001680 1106 1144 SM00320 WD-40 repeat

IPR001680 1148 1184 SM00320 WD-40 repeat

IPR001680 1187 1224 SM00320 WD-40 repeat

ProfileScan IPR000731 285 443 PS50156 Sterol-sensing 5TM box

IPR001680 1073 1233 PS50294 WD-40 repeat

IPR001680 1113 1153 PS50082 WD-40 repeat

ScanRegExp IPR001680 1131 1145 PS00678 WD-40 repeat

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001680	1090	1104	PR00320	WD-40 repeat
IPR001680	1131	1145	PR00320	WD-40 repeat
IPR001680	1171	1185	PR00320	WD-40 repeat
HMMPfam	IPR001680	768	803	PF00400	WD-40 repeat
IPR001680	1068	1103	PF00400	WD-40 repeat
IPR001680	1108	1144	PF00400	WD-40 repeat
IPR001680	1149	1184	PF00400	WD-40 repeat
HMMSmart	IPR001680	766	803	SM00320	WD-40 repeat
IPR001680	954	991	SM00320	WD-40 repeat
IPR001680	1063	1103	SM00320	WD-40 repeat
IPR001680	1106	1144	SM00320	WD-40 repeat
IPR001680	1148	1184	SM00320	WD-40 repeat
IPR001680	1187	1224	SM00320	WD-40 repeat
ProfileScan	IPR000731	285	443	PS50156	Sterol-sensing 5TM box
IPR001680	1073	1233	PS50294	WD-40 repeat
IPR001680	1113	1153	PS50082	WD-40 repeat
ScanRegExp	IPR001680	1131	1145	PS00678	WD-40 repeat

Prediction of transmembrane (TM) segments

Method From To amino acid sequence

SOSUI 23 45 LCASYPIPIILFTGLCILACCYP

281 303 IGIAELIPLVTTYIILFAYIYFS

316 338 LALAAVVTVLSSLLMSVGLCTLF

350 372 FPYLVVVIGLENVLVLTKSVVST

398 420 ATELGIILIGYFTLVPAIQEFCL

424 445 VGLVSDFFLQMLFFTTVLSIDI

710 732 YKVAALGLAAGIVLVLLLLCLYR

773 795 LRGHLMDIECLASDGMLLVSCCL

Method	From	To	amino acid sequence
SOSUI	23	45	LCASYPIPIILFTGLCILACCYP
281	303	IGIAELIPLVTTYIILFAYIYFS
316	338	LALAAVVTVLSSLLMSVGLCTLF
350	372	FPYLVVVIGLENVLVLTKSVVST
398	420	ATELGIILIGYFTLVPAIQEFCL
424	445	VGLVSDFFLQMLFFTTVLSIDI
710	732	YKVAALGLAAGIVLVLLLLCLYR
773	795	LRGHLMDIECLASDGMLLVSCCL

How to obtain mKIAA clone(s) How to obtain anti mKIAA antibodies Back to the ROUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	144 bp
Genome contig ID	gi65519420f_110273398
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- CCTGACTGTAATAATAAACTTTTTTAAAAAACCAC
Flanking genome sequence (153191 - 153240)	----+--------+--------+--------+--------+----* ATTTACCAGGGGCTGAAGTTCTTGTCATTCCACACTCCTCCTAGACCCTT

Gene/Protein Characteristic Table for mKIAA0199

Features of the cloned DNA sequence

Features of the protein sequence