##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BciVI BfrI BlnI BsiWI BspDI
BspLU11I BssHII BstAPI BstBI ClaI
CpoI DrdI Eam1105I EcoT22I FseI
HincII HindII HpaI KspI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SalI SfuI SgrAI SmaI SnaBI
SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 4225 GTGCAC G^TGCAC
ApaLI 4225 GTGCAC G^TGCAC
Asp700I 151 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1558 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3439 GGATCC G^GATCC
BbeI 4267 GGCGCC GGCGC^C
BbrPI 8 CACGTG CAC^GTG
BclI 4379 TGATCA T^GATCA
BsaBI 2220 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1814 TCATGA T^CATGA
Bst1107I 2233 GTATAC GTA^TAC
BstZ17I 2233 GTATAC GTA^TAC
DraI 4970 TTTAAA TTT^AAA
EagI 3956 CGGCCG C^GGCCG
EclXI 3956 CGGCCG C^GGCCG
Eco52I 3956 CGGCCG C^GGCCG
FbaI 4379 TGATCA T^GATCA
KasI 4267 GGCGCC G^GCGCC
MamI 2220 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4267 GGCGCC GG^CGCC
PaeR7I 1762 CTCGAG C^TCGAG
PflFI 1558 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 8 CACGTG CAC^GTG
PmlI 8 CACGTG CAC^GTG
PvuI 1961 CGATCG CGAT^CG
RcaI 1814 TCATGA T^CATGA
SapI 1216 GCTCTTC GCTCTTC(1/4)
SbfI 579 CCTGCAGG CCTGCA^GG
ScaI 1605 AGTACT AGT^ACT
SfiI 1013 GGCCTCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4267 GGCGCC GGC^GCC
Sse8387I 579 CCTGCAGG CCTGCA^GG
Tth111I 1558 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3711 TCTAGA T^CTAGA
XhoI 1762 CTCGAG C^TCGAG
XmnI 151 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1290 GGTACC G^GTACC
2206 GGTACC
AccI 2233 GTATAC GT^[AC][GT]AC
4704 GTCTAC
AflIII 9 ACGTGT A^C[GA][CT]GT
1718 ACGTGT
Aor51HI 860 AGCGCT AGC^GCT
2723 AGCGCT
Asp718I 1290 GGTACC G^GTACC
2206 GGTACC
BalI 2570 TGGCCA TGG^CCA
4396 TGGCCA
BcgI 1640 GCACCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3928 GCAATCTCATCG
BglII 1679 AGATCT A^GATCT
4012 AGATCT
BsaAI 8 CACGTG [CT]AC^GT[GA]
1391 TACGTG
BsmBI 906 GAGACG CGTCTC(1/5)
3297 GAGACG
BsmI 502 GCATTC GAATGC(1/-1)
2365 GCATTC
Eco47III 860 AGCGCT AGC^GCT
2723 AGCGCT
EcoNI 564 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2645 CCTCCACTAGG
EcoRI 304 GAATTC G^AATTC
2673 GAATTC
EcoRV 70 GATATC GAT^ATC
168 GATATC
HindIII 1771 AAGCTT A^AGCTT
4610 AAGCTT
KpnI 1290 GGTACC GGTAC^C
2206 GGTACC
MluNI 2570 TGGCCA TGG^CCA
4396 TGGCCA
MscI 2570 TGGCCA TGG^CCA
4396 TGGCCA
NaeI 3788 GCCGGC GCC^GGC
3958 GCCGGC
NgoMIV 3788 GCCGGC G^CCGGC
3958 GCCGGC
NspI 2436 GCATGC [GA]CATG^[CT]
3784 GCATGC
PflMI 507 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2693 CCAGTTTATGG
SpeI 2665 ACTAGT A^CTAGT
3371 ACTAGT
SphI 2436 GCATGC GCATG^C
3784 GCATGC
Van91I 507 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2693 CCAGTTTATGG
XcmI 2476 CCAGAGGTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2860 CCAGAGCTGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII