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SpecieshumanProduct IDFHC00865Cloning SiteSgfI-PmeISymbolALS2
Alias : ALS2CR6, ALSJ, IAHSP, PLSJDescriptionamyotrophic lateral sclerosis 2 (juvenile), transcript variant 1
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 4971 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1657 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS42800.1 0 99.9 ALS2 CCDS46492.1 1.8e-145 99.7 ALS2 CCDS2743.1 2.8e-68 34.3 ALS2CL CCDS34598.1 7.9e-15 29.5 RSPH10B CCDS43552.1 7.9e-15 29.5 RSPH10B2
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR000408 527 543 PR00633 Regulator of chromosome condensation IPR000408 561 574 PR00633 Regulator of chromosome condensation IPR000408 580 596 PR00633 Regulator of chromosome condensation HMMPfam IPR000408 109 165 PF00415 Regulator of chromosome condensation IPR000408 171 216 PF00415 Regulator of chromosome condensation IPR000408 527 574 PF00415 Regulator of chromosome condensation IPR000408 579 625 PF00415 Regulator of chromosome condensation IPR000219 695 878 PF00621 Dbl homology (DH) domain IPR003409 1049 1070 PF02493 MORN motif IPR003409 1072 1091 PF02493 MORN motif IPR003409 1100 1120 PF02493 MORN motif IPR003409 1123 1139 PF02493 MORN motif IPR003409 1151 1166 PF02493 MORN motif IPR003409 1175 1189 PF02493 MORN motif IPR003409 1198 1217 PF02493 MORN motif IPR003409 1221 1243 PF02493 MORN motif IPR003123 1553 1653 PF02204 Vacuolar sorting protein 9 HMMSmart IPR003409 1047 1068 SM00698 MORN motif IPR003409 1070 1091 SM00698 MORN motif IPR003409 1098 1119 SM00698 MORN motif IPR003409 1121 1142 SM00698 MORN motif IPR003409 1149 1170 SM00698 MORN motif IPR003409 1173 1194 SM00698 MORN motif IPR003409 1196 1217 SM00698 MORN motif IPR003409 1219 1241 SM00698 MORN motif ProfileScan IPR000408 60 109 PS50012 Regulator of chromosome condensation IPR000408 110 168 PS50012 Regulator of chromosome condensation IPR000408 169 219 PS50012 Regulator of chromosome condensation IPR000408 526 577 PS50012 Regulator of chromosome condensation IPR000408 578 628 PS50012 Regulator of chromosome condensation IPR000219 690 885 PS50010 Dbl homology (DH) domain IPR003123 1513 1657 PS51205 Vacuolar sorting protein 9
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065970 1657 205100,606352,606353,607225 99.9 100.0 XP_006712717 1657 205100,606352,606353,607225 99.9 100.0 XP_016860058 1656 205100,606352,606353,607225 99.8 100.0 XP_011509832 1544 205100,606352,606353,607225 99.9 93.2 XP_016860059 1342 205100,606352,606353,607225 99.9 80.6 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21ASDA1563 Download>KIAA1563 4971 bp ATGGACTCAAAGAAGAGAAGCTCAACAGAGGCAGAAGGATCCAAGGAAAGAGGCCTGGTC CATATCTGGCAGGCAGGATCCTTTCCCATAACACCAGAGAGATTGCCAGGCTGGGGAGGA AAGACTGTTTTGCAGGCAGCCCTCGGAGTGAAACATGGAGTTCTTCTGACTGAAGATGGT GAGGTCTACAGCTTTGGGACTCTTCCCTGGAGAAGTGGACCAGTGGAGATTTGTCCAAGT AGCCCCATTCTAGAAAATGCCCTGGTTGGGCAATATGTTATTACTGTGGCAACAGGAAGC TTCCATAGTGGAGCAGTGACAGACAATGGTGTCGCGTACATGTGGGGAGAGAATTCTGCT GGCCAGTGTGCAGTAGCCAACCAGCAGTATGTGCCGGAACCAAATCCTGTCAGCATTGCT GATTCTGAGGCCAGCCCTTTGTTAGCAGTCAGGATTTTACAGTTGGCGTGTGGCGAGGAG CACACTCTGGCATTGTCAATAAGCAGAGAGATTTGGGCATGGGGTACCGGTTGTCAGTTG GGTCTCATTACCACTGCCTTCCCAGTGACAAAGCCGCAAAAGGTAGAACATCTTGCTGGG CGAGTGGTGCTTCAAGTTGCCTGTGGTGCTTTCCACAGCTTAGCCCTTGTACAATGCCTC CCTTCCCAGGATCTGAAGCCAGTCCCAGAACGATGCAACCAGTGCAGCCAGCTCTTGATT ACTATGACTGACAAAGAAGACCATGTGATTATATCAGACAGTCATTGTTGCCCATTAGGT GTGACACTGACAGAATCTCAGGCAGAAAACCATGCCAGCACTGCTCTCAGCCCCTCCACT GAAACCCTTGACAGGCAGGAAGAAGTATTTGAGAACACTCTTGTAGCAAATGATCAGTCT GTTGCTACTGAACTGAATGCAGTAAGTGCTCAGATCACAAGCAGCGATGCCATGTCCTCT CAACAAAATGTCATGGGAACAACTGAAATTTCCTCTGCCAGAAACATACCATCATACCCT GACACCCAAGCAGTCAATGAATACCTACGGAAACTGTCAGATCATTCAGTAAGAGAGGAC TCAGAGCATGGTGAAAAGCCAATGCCATCTCAGCCTCTTTTAGAAGAAGCAATTCCTAAT CTCCACAGCCCGCCTACCACAAGCACCTCAGCCCTAAACAGCCTGGTGGTCTCTTGTGCA TCTGCTGTTGGTGTGAGAGTGGCTGCTACTTATGAAGCTGGTGCCTTGTCACTGAAGAAA GTTATGAACTTTTATAGTACAACCCCTTGTGAAACTGGAGCTCAGGCAGGCAGTAGTGCC ATTGGCCCCGAAGGTTTGAAAGATAGCAGGGAAGAACAGGTTAAACAGGAATCAATGCAA GGAAAGAAAAGTTCAAGTCTTGTGGATATCAGAGAAGAAGAAACAGAGGGAGGCAGTCGA AGACTCTCCCTCCCTGGATTGTTGTCACAAGTTTCCCCCAGGCTCTTAAGAAAGGCTGCA CGGGTGAAAACGAGGACAGTGGTTCTGACCCCCACATACAGTGGAGAAGCAGATGCGCTC CTGCCTTCTCTGAGAACAGAAGTGTGGACCTGGGGGAAAGGGAAGGAAGGGCAGCTGGGG CACGGCGATGTTCTGCCTAGGCTTCAACCGTTGTGTGTAAAATGTCTGGATGGCAAAGAA GTAATCCATCTGGAGGCAGGTGGTTACCATTCTCTTGCACTTACTGCGAAATCCCAGGTT TACTCATGGGGTAGCAATACCTTTGGTCAACTTGGGCATTCCGATTTTCCAACAACAGTT CCTCGTCTTGCAAAGATAAGCAGTGAAAATGGAGTCTGGAGCATAGCTGCAGGCAGGGAT TATTCCCTGTTTTTAGTGGATACAGAAGACTTCCAGCCTGGGTTATATTACAGTGGCCGA CAGGACCCTACAGAAGGTGACAACCTTCCAGAGAATCACAGTGGTTCTAAGACTCCAGTA CTTCTCTCCTGTAGTAAGCTTGGATATATAAGCAGAGTGACAGCAGGAAAAGATAGCTAT TTAGCCTTGGTGGATAAAAACATTATGGGGTATATTGCCAGTCTCCACGAGTTAGCTACT ACAGAAAGACGATTCTATTCAAAACTAAGTGATATCAAATCTCAGATTCTCAGGCCTCTT CTCAGTTTAGAAAATTTGGGCACTACAACTACAGTCCAGCTGTTGCAGGAGGTGGCTAGC CGATTCAGCAAGCTGTGTTACCTCATTGGTCAGCATGGAGCCTCATTGAGCAGCTTCCTT CATGGGGTAAAGGAAGCCAGGAGTTTGGTCATCCTGAAGCATTCAAGTCTCTTCTTGGAT AGTTATACAGAGTATTGCACATCTATTACAAATTTCCTGGTTATGGGAGGATTCCAGCTT CTTGCTAAGCCTGCCATTGATTTCCTAAATAAAAACCAAGAGCTGTTGCAAGATTTGTCA GAAGTGAATGACGAAAACACTCAGTTGATGGAAATACTGAATACTTTGTTTTTCTTGCCA ATCAGACGACTTCATAATTACGCAAAAGTTTTGCTAAAGCTTGCTACTTGTTTTGAAGTG GCATCTCCAGAATATCAGAAACTGCAGGATTCCAGTTCTTGTTATGAGTGTCTTGCTCTC CATCTCGGCAGGAAAAGGAAGGAAGCAGAATACACACTGGGCTTCTGGAAGACCTTCCCC GGAAAAATGACGGATTCCTTGAGGAAGCCAGAGCGTCGACTGCTGTGTGAGAGTAGTAAC CGAGCCCTGTCTCTGCAGCATGCTGGGAGGTTTTCCGTGAATTGGTTCATTCTCTTTAAT GATGCCCTGGTCCATGCCCAGTTCTCCACGCACCATGTTTTCCCTCTGGCCACGCTGTGG GCAGAGCCACTGTCTGAAGAAGCTGGTGGTGTGAATGGCTTAAAGATAACTACACCTGAG GAGCAGTTCACTCTCATTTCATCTACACCCCAGGAAAAGACAAAGTGGCTACGAGCTATA AGCCAAGCCGTAGATCAGGCTTTGAGAGGGATGTCTGATCTCCCCCCTTATGGAAGTGGT AGCAGTGTTCAGAGACAGGAACCACCCATTTCACGCAGTGCCAAATATACTTTCTACAAG GATCCTCGCCTAAAGGATGCCACCTATGATGGACGCTGGCTTTCAGGGAAGCCTCATGGC AGAGGGGTTTTGAAGTGGCCTGATGGAAAGATGTATTCTGGCATGTTCAGGAATGGCTTG GAAGATGGGTATGGAGAATACAGAATCCCAAACAAGGCAATGAACAAAGAAGACCATTAT GTGGGCCATTGGAAAGAAGGAAAAATGTGCGGTCAAGGAGTCTACAGCTATGCTTCTGGT GAAGTATTTGAGGGCTGTTTTCAAGATAATATGCGTCATGGTCATGGTCTTCTACGAAGT GGGAAATTGACGTCCTCTTCTCCTAGTATGTTCATTGGCCAGTGGGTAATGGATAAGAAA GCAGGATATGGTGTCTTTGATGATATCACTAGGGGGGAAAAGTATATGGGAATGTGGCAA GATGATGTGTGTCAAGGGAATGGTGTGGTGGTTACCCAGTTTGGATTATACTACGAGGGC AACTTTCACCTTAATAAAATGATGGGAAATGGGGTTTTGCTTTCCGAAGATGATACTATC TATGAAGGAGAATTTTCAGATGACTGGACTCTTAGTGGAAAGGGAACACTGACTATGCCA AATGGAGACTACATTGAAGGTTATTTTAGTGGAGAATGGGGATCTGGGATAAAAATCACT GGAACCTACTTCAAACCTAGTCTATATGAGAGTGATAAAGACAGACCTAAAGTTTTCAGG AAGCTAGGAAACCTGGCAGTGCCAGCTGATGAGAAGTGGAAAGCGGTGTTTGACGAATGT TGGCGCCAACTGGGCTGTGAGGGCCCAGGCCAAGGGGAAGTTTGGAAAGCATGGGACAAT ATTGCTGTGGCCTTGACCACCAGTCGGCGCCAGCACAGAGACAGTCCAGAAATACTGAGT CGTTCACAGACTCAGACACTAGAGAGTTTGGAATTCATTCCACAGCATGTTGGTGCCTTC TCTGTGGAGAAATATGATGACATCAGGAAATATTTAATAAAGGCCTGTGACACTCCTCTG CACCCCCTGGGCAGGCTTGTGGAGACACTGGTTGCAGTGTATAGAATGACATACGTGGGC GTAGGAGCCAACCGCAGGTTATTGCAGGAGGCTGTAAAGGAGATTAAGTCCTATCTTAAG CGAATTTTCCAGCTGGTGAGGTTCTTATTTCCTGAGCTGCCTGAAGAAGGCAGCACAATT CCTCTCTCTGCTCCTCTGCCAACCGAAAGGAAGTCTTTTTGCACTGGGAAGTCAGATTCC CGATCTGAATCACCAGAGCCAGGTTATGTAGTAACGAGTTCTGGATTATTGCTTCCTGTG CTGCTACCTCGGCTCTACCCACCGCTGTTTATGCTTTATGCTTTGGATAATGATCGCGAG GAAGACATTTACTGGGAATGTGTCCTTCGACTAAATAAGCAGCCAGATATTGCTCTCCTG GGCTTTCTTGGGGTGCAGAGGAAATTTTGGCCAGCAACCTTGTCAATCCTTGGAGAGAGT AAAAAGGTTTTGCCAACCACGAAAGATGCTTGTTTTGCCTCAGCAGTAGAATGTCTGCAG CAGATCAGCACAACATTTACCCCATCAGACAAACTTAAGGTCATCCAGCAGACTTTTGAG GAGATCTCTCAGAGTGTCCTGGCGTCACTCCACGAAGACTTCTTGTGGTCCATGGATGAC TTGTTTCCTGTTTTCTTATATGTGGTGCTACGGGCCAGGATTAGGAATTTAGGCTCTGAG GTACACCTCATTGAGGATCTAATGGACCCCTATCTTCAGCATGGGGAACAGGGTATAATG TTCACCACCTTGAAGGCATGTTACTACCAGATTCAGCGTGAGAAGCTTAAC
Cloned ORF protein sequence for pFN21ASDA1563 Download>KIAA1563 1657 aa MDSKKRSSTEAEGSKERGLVHIWQAGSFPITPERLPGWGGKTVLQAALGVKHGVLLTEDG EVYSFGTLPWRSGPVEICPSSPILENALVGQYVITVATGSFHSGAVTDNGVAYMWGENSA GQCAVANQQYVPEPNPVSIADSEASPLLAVRILQLACGEEHTLALSISREIWAWGTGCQL GLITTAFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQCLPSQDLKPVPERCNQCSQLLI TMTDKEDHVIISDSHCCPLGVTLTESQAENHASTALSPSTETLDRQEEVFENTLVANDQS VATELNAVSAQITSSDAMSSQQNVMGTTEISSARNIPSYPDTQAVNEYLRKLSDHSVRED SEHGEKPMPSQPLLEEAIPNLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKK VMNFYSTTPCETGAQAGSSAIGPEGLKDSREEQVKQESMQGKKSSSLVDIREEETEGGSR RLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLG HGDVLPRLQPLCVKCLDGKEVIHLEAGGYHSLALTAKSQVYSWGSNTFGQLGHSDFPTTV PRLAKISSENGVWSIAAGRDYSLFLVDTEDFQPGLYYSGRQDPTEGDNLPENHSGSKTPV LLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFYSKLSDIKSQILRPL LSLENLGTTTTVQLLQEVASRFSKLCYLIGQHGASLSSFLHGVKEARSLVILKHSSLFLD SYTEYCTSITNFLVMGGFQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNTLFFLP IRRLHNYAKVLLKLATCFEVASPEYQKLQDSSSCYECLALHLGRKRKEAEYTLGFWKTFP GKMTDSLRKPERRLLCESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLW AEPLSEEAGGVNGLKITTPEEQFTLISSTPQEKTKWLRAISQAVDQALRGMSDLPPYGSG SSVQRQEPPISRSAKYTFYKDPRLKDATYDGRWLSGKPHGRGVLKWPDGKMYSGMFRNGL EDGYGEYRIPNKAMNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRS GKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDVCQGNGVVVTQFGLYYEG NFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLSGKGTLTMPNGDYIEGYFSGEWGSGIKIT GTYFKPSLYESDKDRPKVFRKLGNLAVPADEKWKAVFDECWRQLGCEGPGQGEVWKAWDN IAVALTTSRRQHRDSPEILSRSQTQTLESLEFIPQHVGAFSVEKYDDIRKYLIKACDTPL HPLGRLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTI PLSAPLPTERKSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDRE EDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPTTKDACFASAVECLQ QISTTFTPSDKLKVIQQTFEEISQSVLASLHEDFLWSMDDLFPVFLYVVLRARIRNLGSE VHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN
Nucleotide Sequence (with vector) for pFN21ASDA1563 Download>pFN21ASDA1563 9700 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGACTCAAAGAAGAG AAGCTCAACAGAGGCAGAAGGATCCAAGGAAAGAGGCCTGGTCCATATCTGGCAGGCAGG ATCCTTTCCCATAACACCAGAGAGATTGCCAGGCTGGGGAGGAAAGACTGTTTTGCAGGC AGCCCTCGGAGTGAAACATGGAGTTCTTCTGACTGAAGATGGTGAGGTCTACAGCTTTGG GACTCTTCCCTGGAGAAGTGGACCAGTGGAGATTTGTCCAAGTAGCCCCATTCTAGAAAA TGCCCTGGTTGGGCAATATGTTATTACTGTGGCAACAGGAAGCTTCCATAGTGGAGCAGT GACAGACAATGGTGTCGCGTACATGTGGGGAGAGAATTCTGCTGGCCAGTGTGCAGTAGC CAACCAGCAGTATGTGCCGGAACCAAATCCTGTCAGCATTGCTGATTCTGAGGCCAGCCC TTTGTTAGCAGTCAGGATTTTACAGTTGGCGTGTGGCGAGGAGCACACTCTGGCATTGTC AATAAGCAGAGAGATTTGGGCATGGGGTACCGGTTGTCAGTTGGGTCTCATTACCACTGC CTTCCCAGTGACAAAGCCGCAAAAGGTAGAACATCTTGCTGGGCGAGTGGTGCTTCAAGT TGCCTGTGGTGCTTTCCACAGCTTAGCCCTTGTACAATGCCTCCCTTCCCAGGATCTGAA GCCAGTCCCAGAACGATGCAACCAGTGCAGCCAGCTCTTGATTACTATGACTGACAAAGA AGACCATGTGATTATATCAGACAGTCATTGTTGCCCATTAGGTGTGACACTGACAGAATC TCAGGCAGAAAACCATGCCAGCACTGCTCTCAGCCCCTCCACTGAAACCCTTGACAGGCA GGAAGAAGTATTTGAGAACACTCTTGTAGCAAATGATCAGTCTGTTGCTACTGAACTGAA TGCAGTAAGTGCTCAGATCACAAGCAGCGATGCCATGTCCTCTCAACAAAATGTCATGGG AACAACTGAAATTTCCTCTGCCAGAAACATACCATCATACCCTGACACCCAAGCAGTCAA TGAATACCTACGGAAACTGTCAGATCATTCAGTAAGAGAGGACTCAGAGCATGGTGAAAA GCCAATGCCATCTCAGCCTCTTTTAGAAGAAGCAATTCCTAATCTCCACAGCCCGCCTAC CACAAGCACCTCAGCCCTAAACAGCCTGGTGGTCTCTTGTGCATCTGCTGTTGGTGTGAG AGTGGCTGCTACTTATGAAGCTGGTGCCTTGTCACTGAAGAAAGTTATGAACTTTTATAG TACAACCCCTTGTGAAACTGGAGCTCAGGCAGGCAGTAGTGCCATTGGCCCCGAAGGTTT GAAAGATAGCAGGGAAGAACAGGTTAAACAGGAATCAATGCAAGGAAAGAAAAGTTCAAG TCTTGTGGATATCAGAGAAGAAGAAACAGAGGGAGGCAGTCGAAGACTCTCCCTCCCTGG ATTGTTGTCACAAGTTTCCCCCAGGCTCTTAAGAAAGGCTGCACGGGTGAAAACGAGGAC AGTGGTTCTGACCCCCACATACAGTGGAGAAGCAGATGCGCTCCTGCCTTCTCTGAGAAC AGAAGTGTGGACCTGGGGGAAAGGGAAGGAAGGGCAGCTGGGGCACGGCGATGTTCTGCC TAGGCTTCAACCGTTGTGTGTAAAATGTCTGGATGGCAAAGAAGTAATCCATCTGGAGGC AGGTGGTTACCATTCTCTTGCACTTACTGCGAAATCCCAGGTTTACTCATGGGGTAGCAA TACCTTTGGTCAACTTGGGCATTCCGATTTTCCAACAACAGTTCCTCGTCTTGCAAAGAT AAGCAGTGAAAATGGAGTCTGGAGCATAGCTGCAGGCAGGGATTATTCCCTGTTTTTAGT GGATACAGAAGACTTCCAGCCTGGGTTATATTACAGTGGCCGACAGGACCCTACAGAAGG TGACAACCTTCCAGAGAATCACAGTGGTTCTAAGACTCCAGTACTTCTCTCCTGTAGTAA GCTTGGATATATAAGCAGAGTGACAGCAGGAAAAGATAGCTATTTAGCCTTGGTGGATAA AAACATTATGGGGTATATTGCCAGTCTCCACGAGTTAGCTACTACAGAAAGACGATTCTA TTCAAAACTAAGTGATATCAAATCTCAGATTCTCAGGCCTCTTCTCAGTTTAGAAAATTT GGGCACTACAACTACAGTCCAGCTGTTGCAGGAGGTGGCTAGCCGATTCAGCAAGCTGTG TTACCTCATTGGTCAGCATGGAGCCTCATTGAGCAGCTTCCTTCATGGGGTAAAGGAAGC CAGGAGTTTGGTCATCCTGAAGCATTCAAGTCTCTTCTTGGATAGTTATACAGAGTATTG CACATCTATTACAAATTTCCTGGTTATGGGAGGATTCCAGCTTCTTGCTAAGCCTGCCAT TGATTTCCTAAATAAAAACCAAGAGCTGTTGCAAGATTTGTCAGAAGTGAATGACGAAAA CACTCAGTTGATGGAAATACTGAATACTTTGTTTTTCTTGCCAATCAGACGACTTCATAA TTACGCAAAAGTTTTGCTAAAGCTTGCTACTTGTTTTGAAGTGGCATCTCCAGAATATCA GAAACTGCAGGATTCCAGTTCTTGTTATGAGTGTCTTGCTCTCCATCTCGGCAGGAAAAG GAAGGAAGCAGAATACACACTGGGCTTCTGGAAGACCTTCCCCGGAAAAATGACGGATTC CTTGAGGAAGCCAGAGCGTCGACTGCTGTGTGAGAGTAGTAACCGAGCCCTGTCTCTGCA GCATGCTGGGAGGTTTTCCGTGAATTGGTTCATTCTCTTTAATGATGCCCTGGTCCATGC CCAGTTCTCCACGCACCATGTTTTCCCTCTGGCCACGCTGTGGGCAGAGCCACTGTCTGA AGAAGCTGGTGGTGTGAATGGCTTAAAGATAACTACACCTGAGGAGCAGTTCACTCTCAT TTCATCTACACCCCAGGAAAAGACAAAGTGGCTACGAGCTATAAGCCAAGCCGTAGATCA GGCTTTGAGAGGGATGTCTGATCTCCCCCCTTATGGAAGTGGTAGCAGTGTTCAGAGACA GGAACCACCCATTTCACGCAGTGCCAAATATACTTTCTACAAGGATCCTCGCCTAAAGGA TGCCACCTATGATGGACGCTGGCTTTCAGGGAAGCCTCATGGCAGAGGGGTTTTGAAGTG GCCTGATGGAAAGATGTATTCTGGCATGTTCAGGAATGGCTTGGAAGATGGGTATGGAGA ATACAGAATCCCAAACAAGGCAATGAACAAAGAAGACCATTATGTGGGCCATTGGAAAGA AGGAAAAATGTGCGGTCAAGGAGTCTACAGCTATGCTTCTGGTGAAGTATTTGAGGGCTG TTTTCAAGATAATATGCGTCATGGTCATGGTCTTCTACGAAGTGGGAAATTGACGTCCTC TTCTCCTAGTATGTTCATTGGCCAGTGGGTAATGGATAAGAAAGCAGGATATGGTGTCTT TGATGATATCACTAGGGGGGAAAAGTATATGGGAATGTGGCAAGATGATGTGTGTCAAGG GAATGGTGTGGTGGTTACCCAGTTTGGATTATACTACGAGGGCAACTTTCACCTTAATAA AATGATGGGAAATGGGGTTTTGCTTTCCGAAGATGATACTATCTATGAAGGAGAATTTTC AGATGACTGGACTCTTAGTGGAAAGGGAACACTGACTATGCCAAATGGAGACTACATTGA AGGTTATTTTAGTGGAGAATGGGGATCTGGGATAAAAATCACTGGAACCTACTTCAAACC TAGTCTATATGAGAGTGATAAAGACAGACCTAAAGTTTTCAGGAAGCTAGGAAACCTGGC AGTGCCAGCTGATGAGAAGTGGAAAGCGGTGTTTGACGAATGTTGGCGCCAACTGGGCTG TGAGGGCCCAGGCCAAGGGGAAGTTTGGAAAGCATGGGACAATATTGCTGTGGCCTTGAC CACCAGTCGGCGCCAGCACAGAGACAGTCCAGAAATACTGAGTCGTTCACAGACTCAGAC ACTAGAGAGTTTGGAATTCATTCCACAGCATGTTGGTGCCTTCTCTGTGGAGAAATATGA TGACATCAGGAAATATTTAATAAAGGCCTGTGACACTCCTCTGCACCCCCTGGGCAGGCT TGTGGAGACACTGGTTGCAGTGTATAGAATGACATACGTGGGCGTAGGAGCCAACCGCAG GTTATTGCAGGAGGCTGTAAAGGAGATTAAGTCCTATCTTAAGCGAATTTTCCAGCTGGT GAGGTTCTTATTTCCTGAGCTGCCTGAAGAAGGCAGCACAATTCCTCTCTCTGCTCCTCT GCCAACCGAAAGGAAGTCTTTTTGCACTGGGAAGTCAGATTCCCGATCTGAATCACCAGA GCCAGGTTATGTAGTAACGAGTTCTGGATTATTGCTTCCTGTGCTGCTACCTCGGCTCTA CCCACCGCTGTTTATGCTTTATGCTTTGGATAATGATCGCGAGGAAGACATTTACTGGGA ATGTGTCCTTCGACTAAATAAGCAGCCAGATATTGCTCTCCTGGGCTTTCTTGGGGTGCA GAGGAAATTTTGGCCAGCAACCTTGTCAATCCTTGGAGAGAGTAAAAAGGTTTTGCCAAC CACGAAAGATGCTTGTTTTGCCTCAGCAGTAGAATGTCTGCAGCAGATCAGCACAACATT TACCCCATCAGACAAACTTAAGGTCATCCAGCAGACTTTTGAGGAGATCTCTCAGAGTGT CCTGGCGTCACTCCACGAAGACTTCTTGTGGTCCATGGATGACTTGTTTCCTGTTTTCTT ATATGTGGTGCTACGGGCCAGGATTAGGAATTTAGGCTCTGAGGTACACCTCATTGAGGA TCTAATGGACCCCTATCTTCAGCATGGGGAACAGGGTATAATGTTCACCACCTTGAAGGC ATGTTACTACCAGATTCAGCGTGAGAAGCTTAACTACGTAGTTTAAACGAATTCGGGCTC GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT
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