ALG10 ORF clone pFN21AE6435

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6435

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Clone info.

Species

human

Clone Name

pFN21AE6435 Sequence (with vector) Restriction Map

Product ID

FHC29674

ORF Sequence

DNA sequence (1419 bp)
Protein sequence (473 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6435

Symbol

ALG10

Alias : ALG10A, DIE2, KCR1

Description

ALG10, alpha-1,2-glucosyltransferase

Original Clone ID

oc96435

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1419 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 473 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41769.1 2e-215 100.0 ALG10 CCDS31772.1 2.5e-207 96.0 ALG10B CCDS76548.1 3.9e-50 95.9 ALG10B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007006 29 427 PF04922 Glycosyltransferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 7 YYFSAALSCTFLVSCLLFSAFSR 29 SECONDARY 23 2 62 ITTLPGLYLVSIGVIKPAIWIFG 84 PRIMARY 23 3 93 IGMLRFVNLLFSVGNFYLLYLLF 115 PRIMARY 23 4 129 QRVLSTLTLAVFPTLYFFNFLY 150 SECONDARY 22 5 156 SMFFTLFAYLMCLY 169 SECONDARY 14 6 187 QTNIIWAVFCAGNVIAQKLTEAW 209 SECONDARY 23 7 224 KGPFAEFRKILQFLLAYSMSFKN 246 SECONDARY 23 8 252 LLTWPYILLGFLFCAFVVVNGGI 274 PRIMARY 23 9 285 CLHFPQLFYFFSFTLFFSFPHLL 307 SECONDARY 23 10 325 LFFVVTLVSVFLVWKFTYAHKYL 347 PRIMARY 23 11 366 YETVKYLLVPAYIFAGWSIADSL 388 SECONDARY 23 12 401 ICLFTVIVPQKLLEFRYFILPYV 423 PRIMARY 23 13 439 CELSCYAVVNFITFFIFLNKTFQ 461 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_116223 473 603313,613688 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6435 Download

>KIEE6435  1419 bp
ATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTATCC
TGCCTCCTCTTCTCCGCCTTCAGCCGGGCGTTGCGAGAGCCCTACATGGACGAGATCTTC
CACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCC
ATGATTACTACATTACCTGGCTTGTACCTGGTGTCAATTGGAGTGATCAAACCTGCCATT
TGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTGGGATGCTCAGATTTGTTAAT
CTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCTGCAAGGTACAACCC
AGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT
CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACT
CTTTTTGCGTATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTT
TGTGGCTTCATGTTTCGGCAAACAAATATCATCTGGGCTGTCTTCTGTGCAGGAAATGTC
ATTGCACAAAAGTTAACGGAGGCTTGGAAAACTGAGCTACAAAAGAAGGAAGACAGACTT
CCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTCTTCAGTTTCTTTTGGCTTAT
TCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCTTCTGACTTGGCCCTACATCCTTCTG
GGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT
AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTT
TTTTCCTTTCCTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGG
AAACGTAGAATTCTGTTTTTTGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTC
ACTTATGCTCATAAATACTTGCTAGCAGACAATAGACATTATACTTTCTATGTGTGGAAA
AGAGTTTTTCAAAGATATGAAACTGTAAAATATTTGTTAGTTCCAGCCTATATATTTGCT
GGTTGGAGTATAGCTGACTCATTGAAATCAAAGTCAATTTTTTGGAATTTAATGTTTTTC
ATATGCTTGTTCACTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA
CCTTATGTCATTTATAGGCTTAACATACCTCTGCCTCCCACATCCAGACTCATTTGTGAA
CTGAGCTGCTATGCAGTTGTTAATTTCATAACTTTTTTCATCTTTCTGAACAAGACTTTT
CAGTGGCCAAATAGTCAGGACATTCAAAGGTTTATGTGG

ORF Protein

Cloned ORF protein sequence for pFN21AE6435 Download

>KIEE6435  473 aa
MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDP
MITTLPGLYLVSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFCKVQP
RNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGF
CGFMFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKEDRLPPIKGPFAEFRKILQFLLAY
SMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVIGDRSSHEACLHFPQLFYFFSFTLF
FSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSVFLVWKFTYAHKYLLADNRHYTFYVWK
RVFQRYETVKYLLVPAYIFAGWSIADSLKSKSIFWNLMFFICLFTVIVPQKLLEFRYFIL
PYVIYRLNIPLPPTSRLICELSCYAVVNFITFFIFLNKTFQWPNSQDIQRFMW

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6435 Download

>pFN21AE6435  6124 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGCAGCTGGAAGGTTACTATTTCTCGGCCGC
CTTGAGCTGTACCTTTTTAGTATCCTGCCTCCTCTTCTCCGCCTTCAGCCGGGCGTTGCG
AGAGCCCTACATGGACGAGATCTTCCACCTGCCTCAGGCGCAGCGCTACTGTGAGGGCCA
TTTCTCCCTTTCCCAGTGGGATCCCATGATTACTACATTACCTGGCTTGTACCTGGTGTC
AATTGGAGTGATCAAACCTGCCATTTGGATCTTTGGATGGTCTGAACATGTTGTCTGCTC
CATTGGGATGCTCAGATTTGTTAATCTTCTCTTCAGTGTTGGCAACTTCTATTTACTATA
TTTGCTTTTCTGCAAGGTACAACCCAGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTT
GTCAACATTAACACTAGCAGTATTTCCAACACTTTATTTTTTTAACTTCCTTTATTATAC
AGAAGCAGGATCTATGTTTTTTACTCTTTTTGCGTATTTGATGTGTCTTTATGGAAATCA
TAAAACTTCAGCCTTCCTTGGATTTTGTGGCTTCATGTTTCGGCAAACAAATATCATCTG
GGCTGTCTTCTGTGCAGGAAATGTCATTGCACAAAAGTTAACGGAGGCTTGGAAAACTGA
GCTACAAAAGAAGGAAGACAGACTTCCACCTATTAAAGGACCATTTGCAGAATTCAGAAA
AATTCTTCAGTTTCTTTTGGCTTATTCCATGTCCTTTAAAAACTTGAGTATGCTTTTGCT
TCTGACTTGGCCCTACATCCTTCTGGGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGG
TGGAATTGTTATTGGCGATCGGAGTAGTCATGAAGCCTGTCTTCATTTTCCTCAACTATT
CTACTTTTTTTCATTTACTCTCTTTTTTTCCTTTCCTCATCTCCTGTCTCCTAGCAAAAT
TAAGACTTTTCTTTCCTTAGTTTGGAAACGTAGAATTCTGTTTTTTGTGGTTACCTTAGT
CTCTGTGTTTTTAGTTTGGAAATTCACTTATGCTCATAAATACTTGCTAGCAGACAATAG
ACATTATACTTTCTATGTGTGGAAAAGAGTTTTTCAAAGATATGAAACTGTAAAATATTT
GTTAGTTCCAGCCTATATATTTGCTGGTTGGAGTATAGCTGACTCATTGAAATCAAAGTC
AATTTTTTGGAATTTAATGTTTTTCATATGCTTGTTCACTGTTATAGTTCCTCAGAAACT
GCTGGAATTTCGTTACTTCATTTTACCTTATGTCATTTATAGGCTTAACATACCTCTGCC
TCCCACATCCAGACTCATTTGTGAACTGAGCTGCTATGCAGTTGTTAATTTCATAACTTT
TTTCATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCAGGACATTCAAAGGTTTAT
GTGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT