METTL2A ORF clone pFN21AE1857

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Halotag ORF Clone pFN21AE1857

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Clone info.

Species

human

Clone Name

pFN21AE1857 Sequence (with vector) Restriction Map

Product ID

FHC30744

ORF Sequence

DNA sequence (1134 bp)
Protein sequence (378 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590991]

Gene

KIEE1857

Symbol

METTL2A

Alias : METTL2

Description

methyltransferase like 2A

Original Clone ID

cs02757

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1134 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 378 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45752.1 8.7e-165 99.7 METTL2A CCDS5803.2 9.9e-164 98.7 METTL2B CCDS2242.2 2.6e-81 55.4 METTL8 CCDS82532.1 5.9e-48 49.2 METTL8 CCDS82531.1 3.6e-41 47.9 METTL8 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013217 185 287 PF08242 Methyltransferase type 12
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 249 VPKGSLDIIILIFVLSAIVPDKM 271 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060866 378 607846 98.7 100.0 NP_001308084 407 609525 55.4 93.7 NP_001308083 407 609525 55.4 93.7 NP_079046 407 609525 55.4 93.7 NP_001308085 407 609525 55.4 93.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1857 Download

>KIEE1857  1134 bp
ATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAGTCCTCGCCGATAAGAGGCAGCAGTTC
GGAAGCCGGTTCCTGAGAGATCCGGCGCGCGTCTTCCACCACAATGCCTGGGACAATGTG
GAGTGGTCGGAAGAGCAAGCCGCGGCGGCGGAGAGAAAAGTCCAGGAGAACAGTATCCAG
CGGGTGTGCCAGGAGAAACAAGTTGATTATGAGATCAATGCCCACAAATACTGGAATGAC
TTCTACAAAATCCACGAAAATGGGTTTTTCAAGGATAGACATTGGCTTTTTACCGAATTC
CCTGAGCTGGCACCTAGCCAAAATCAAAATCATTTGAAGGACTGGTTCTTGGAGAACAAG
AGTGAAGTACCTGAATGTAGAAACAATGAGGATGGACCTGGTTTAATAATGGAAGAACAG
CACAAGTGTTCTTCGAAGAGCCTTGAACATAAAACACAGACACCTCCTGTGGAGGAGAAT
GTAACTCAGAAAATTAGTGACCTGGAAATTTGTGCTGATGAGTTTCCTGGATCCTCAGCC
ACCTACCGAATACTGGAGGTTGGCTGTGGTGTGGGAAACACAGTCTTTCCAATTTTACAA
ACGAACAATGACCCAGGACTCTTTGTTTATTGCTGTGATTTTTCTTCCACAGCTATAGAA
CTGGTCCAGACAAATTCAGAATATGATCCTTCTCGGTGTTTTGCCTTTGTTCACGACCTG
TGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGGCAGTCTTGATATTATCATTCTCATA
TTTGTTCTTTCAGCAATTGTTCCAGACAAGATGCAGAAGGCTATCAACAGGCTGAGCAGG
CTTCTGAAACCTGGCGGGATGATGCTTCTGCGAGATTACGGCCGCTATGACATGGCTCAG
CTTCGGTTTAAAAAAGGTCAGTGTCTATCTGGAAATTTCTACGTGAGAGGTGATGGAACC
AGAGTTTACTTCTTCACACAAGAGGAACTGGACACGCTTTTCACCACTGCTGGACTGGAA
AAAGTTCAGAACCTGGTGGATCGCCGACTGCAGGTGAACCGAGGAAAGCAACTGACAATG
TACCGGGTTTGGATTCAGTGCAAATACTGCAAGCCCCTTCTGTCCAGCACCAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE1857 Download

>KIEE1857  378 aa
MAGSYPEGAPAVLADKRQQFGSRFLRDPARVFHHNAWDNVEWSEEQAAAAERKVQENSIQ
RVCQEKQVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENK
SEVPECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSA
TYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDL
CDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMMLLRDYGRYDMAQ
LRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTM
YRVWIQCKYCKPLLSSTS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1857 Download

>pFN21AE1857  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGGCTCCTACCCTGAAGGTGCACCTGCAGT
CCTCGCCGATAAGAGGCAGCAGTTCGGAAGCCGGTTCCTGAGAGATCCGGCGCGCGTCTT
CCACCACAATGCCTGGGACAATGTGGAGTGGTCGGAAGAGCAAGCCGCGGCGGCGGAGAG
AAAAGTCCAGGAGAACAGTATCCAGCGGGTGTGCCAGGAGAAACAAGTTGATTATGAGAT
CAATGCCCACAAATACTGGAATGACTTCTACAAAATCCACGAAAATGGGTTTTTCAAGGA
TAGACATTGGCTTTTTACCGAATTCCCTGAGCTGGCACCTAGCCAAAATCAAAATCATTT
GAAGGACTGGTTCTTGGAGAACAAGAGTGAAGTACCTGAATGTAGAAACAATGAGGATGG
ACCTGGTTTAATAATGGAAGAACAGCACAAGTGTTCTTCGAAGAGCCTTGAACATAAAAC
ACAGACACCTCCTGTGGAGGAGAATGTAACTCAGAAAATTAGTGACCTGGAAATTTGTGC
TGATGAGTTTCCTGGATCCTCAGCCACCTACCGAATACTGGAGGTTGGCTGTGGTGTGGG
AAACACAGTCTTTCCAATTTTACAAACGAACAATGACCCAGGACTCTTTGTTTATTGCTG
TGATTTTTCTTCCACAGCTATAGAACTGGTCCAGACAAATTCAGAATATGATCCTTCTCG
GTGTTTTGCCTTTGTTCACGACCTGTGTGATGAAGAGAAGAGTTACCCAGTGCCCAAGGG
CAGTCTTGATATTATCATTCTCATATTTGTTCTTTCAGCAATTGTTCCAGACAAGATGCA
GAAGGCTATCAACAGGCTGAGCAGGCTTCTGAAACCTGGCGGGATGATGCTTCTGCGAGA
TTACGGCCGCTATGACATGGCTCAGCTTCGGTTTAAAAAAGGTCAGTGTCTATCTGGAAA
TTTCTACGTGAGAGGTGATGGAACCAGAGTTTACTTCTTCACACAAGAGGAACTGGACAC
GCTTTTCACCACTGCTGGACTGGAAAAAGTTCAGAACCTGGTGGATCGCCGACTGCAGGT
GAACCGAGGAAAGCAACTGACAATGTACCGGGTTTGGATTCAGTGCAAATACTGCAAGCC
CCTTCTGTCCAGCACCAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT