ECI1 ORF clone pFN21AE1012

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Halotag ORF Clone pFN21AE1012

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Clone info.

Species

human

Clone Name

pFN21AE1012 Sequence (with vector) Restriction Map

Product ID

FHC11848

Flexi Clone PID

FXC11848

Original Clone PID

ORK11848

ORF Sequence

DNA sequence (906 bp)
Protein sequence (302 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1012

Symbol

ECI1

Alias : DCI

Description

enoyl-CoA delta isomerase 1, transcript variant 1

Original Clone ID

bn02161

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 906 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 302 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10464.1 2.8e-130 100.0 ECI1 CCDS58410.1 1.1e-72 94.4 ECI1 CCDS6689.1 1.1e-11 25.6 AUH CCDS1721.1 2.1e-11 29.1 HADHA The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001753 58 228 PF00378 Crotonase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001910 302 600305 100.0 100.0 NP_001171500 285 600305 94.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1012 Download

>KIEE1012  906 bp
ATGGCGCTGGTGGCTTCTGTGCGAGTCCCGGCGCGCGTTCTGCTCCGGGCGGGGGCCCGG
CTCCCGGGCGCGGCCCTCGGGCGGACGGAGCGGGCGGCCGGCGGCGGAGACGGCGCGCGG
CGCTTCGGGAGCCAGCGGGTGCTGGTGGAGCCGGACGCGGGCGCAGGGGTCGCTGTGATG
AAATTCAAGAACCCCCCAGTGAACAGCCTGAGCCTGGAGTTTCTGACGGAGCTGGTCATC
AGCCTGGAGAAGCTGGAGAATGACAAGAGCTTCCGCGGTGTCATTCTGACCTCGGACCGC
CCGGGTGTCTTCTCGGCCGGCCTGGACCTGACGGAGATGTGTGGGAGGAGCCCCGCCCAC
TACGCTGGGTACTGGAAGGCCGTTCAGGAGCTGTGGCTGCGGTTGTACCAGTCCAACCTG
GTGCTGGTCTCCGCCATCAACGGAGCCTGCCCCGCTGGAGGCTGCCTGGTGGCCCTGACC
TGTGACTACCGCATCCTGGCGGACAACCCCAGGTACTGCATAGGACTCAATGAGACCCAG
CTGGGCATCATCGCCCCTTTCTGGTTGAAAGACACCCTGGAGAACACCATCGGGCACCGG
GCGGCGGAGCGTGCCCTGCAGCTGGGGCTGCTCTTCCCGCCGGCGGAGGCCCTGCAGGTG
GGCATAGTGGACCAGGTGGTCCCGGAGGAGCAGGTGCAGAGCACTGCGCTGTCAGCGATA
GCCCAGTGGATGGCCATTCCAGACCATGCTCGACAGCTGACCAAGGCCATGATGCGAAAG
GCCACGGCCAGCCGCCTGGTCACGCAGCGCGATGCGGACGTGCAGAACTTCGTCAGCTTC
ATCTCCAAAGACTCCATCCAGAAGTCCCTGCAGATGTACTTAGAGAGGCTCAAAGAAGAA
AAAGGC

ORF Protein

Cloned ORF protein sequence for pFN21AE1012 Download

>KIEE1012  302 aa
MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVM
KFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAH
YAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRYCIGLNETQ
LGIIAPFWLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAI
AQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEE
KG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1012 Download

>pFN21AE1012  5611 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGCTGGTGGCTTCTGTGCGAGTCCCGGCGCG
CGTTCTGCTCCGGGCGGGGGCCCGGCTCCCGGGCGCGGCCCTCGGGCGGACGGAGCGGGC
GGCCGGCGGCGGAGACGGCGCGCGGCGCTTCGGGAGCCAGCGGGTGCTGGTGGAGCCGGA
CGCGGGCGCAGGGGTCGCTGTGATGAAATTCAAGAACCCCCCAGTGAACAGCCTGAGCCT
GGAGTTTCTGACGGAGCTGGTCATCAGCCTGGAGAAGCTGGAGAATGACAAGAGCTTCCG
CGGTGTCATTCTGACCTCGGACCGCCCGGGTGTCTTCTCGGCCGGCCTGGACCTGACGGA
GATGTGTGGGAGGAGCCCCGCCCACTACGCTGGGTACTGGAAGGCCGTTCAGGAGCTGTG
GCTGCGGTTGTACCAGTCCAACCTGGTGCTGGTCTCCGCCATCAACGGAGCCTGCCCCGC
TGGAGGCTGCCTGGTGGCCCTGACCTGTGACTACCGCATCCTGGCGGACAACCCCAGGTA
CTGCATAGGACTCAATGAGACCCAGCTGGGCATCATCGCCCCTTTCTGGTTGAAAGACAC
CCTGGAGAACACCATCGGGCACCGGGCGGCGGAGCGTGCCCTGCAGCTGGGGCTGCTCTT
CCCGCCGGCGGAGGCCCTGCAGGTGGGCATAGTGGACCAGGTGGTCCCGGAGGAGCAGGT
GCAGAGCACTGCGCTGTCAGCGATAGCCCAGTGGATGGCCATTCCAGACCATGCTCGACA
GCTGACCAAGGCCATGATGCGAAAGGCCACGGCCAGCCGCCTGGTCACGCAGCGCGATGC
GGACGTGCAGAACTTCGTCAGCTTCATCTCCAAAGACTCCATCCAGAAGTCCCTGCAGAT
GTACTTAGAGAGGCTCAAAGAAGAAAAAGGCGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT