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SpecieshumanProduct IDFXC26313Cloning SiteSgfI-PmeIGenBank AccessionGeneKIEE2523SymbolATP8B1
Alias : ATPIC, BRIC, FIC1, ICP1, PFIC, PFIC1DescriptionATPase, aminophospholipid transporter, class I, type 8B, member 1Original Clone IDcp01651NoteE.coli strain DH10B seems suitable for preparation of this clone, but the growth of the transformant is slow. No transformants could be obtained using DH5 alpha.
Length: 3753 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1251 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS11965.1 0 99.9 ATP8B1 CCDS45901.1 3.2e-196 47.0 ATP8B3 CCDS54196.1 5.3e-149 47.3 ATP8B3 CCDS41873.1 8.4e-115 41.2 ATP8A2 CCDS32238.1 8.4e-95 55.0 ATP8B4
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR001757 452 466 PR00119 ATPase IPR001757 794 804 PR00119 ATPase IPR001757 891 910 PR00119 ATPase HMMPfam IPR008250 150 411 PF00122 ATPase IPR005834 450 743 PF00702 Haloacid dehalogenase-like hydrolase HMMTigr IPR006539 92 813 TIGR01652 ATPase IPR001757 863 976 TIGR01494 ATPase
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 130 LQAVPQISTLAWYTTLVPLLVVL 152 PRIMARY 23 2 337 MNYMVYTIFVVLILLSAGLAIGH 359 PRIMARY 23 3 386 FLIFWGYIIVLNTMVPISLYVSV 408 PRIMARY 23 4 982 DWFITLYNVLYTSLPVLLMGLLD 1004 SECONDARY 23 5 1036 SLLHGVLTSMILFFIPLGAYLQT 1058 PRIMARY 23 6 1071 SFAVTIASALVITVNFQI 1088 SECONDARY 18 7 1095 WTFVNAFSIFGSIALYFGIMFDF 1117 PRIMARY 23 8 1147 LTIILTVAVCLLPVVAIRFLSMT 1169 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005594 1251 147480,211600,243300,602397 100.0 100.0 XP_011524324 1251 147480,211600,243300,602397 99.9 100.0 XP_006722544 1251 147480,211600,243300,602397 99.9 100.0 XP_011524325 1213 147480,211600,243300,602397 96.9 100.0 XP_011526009 1301 605866 47.9 93.3 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pF1KE2523 Download>KIEE2523 3753 bp ATGAGTACAGAAAGAGACTCAGAAACGACATTTGACGAGGATTCTCAGCCTAATGACGAA GTGGTTCCCTACAGTGATGATGAAACAGAAGATGAACTTGATGACCAGGGGTCTGCTGTT GAACCAGAACAAAACCGAGTCAACAGGGAAGCAGAGGAGAACCGGGAGCCATTCAGAAAA GAATGTACATGGCAAGTCAAAGCAAACGATCGCAAGTACCACGAACAACCTCACTTTATG AACACAAAATTCTTGTGTATTAAGGAGAGTAAATATGCGAATAATGCAATTAAAACATAC AAGTACAACGCATTTACCTTTATACCAATGAATCTGTTTGAGCAGTTTAAGAGAGCAGCC AATTTATATTTCCTGGCTCTTCTTATCTTACAGGCAGTTCCTCAAATCTCTACCCTGGCT TGGTACACCACACTAGTGCCCCTGCTTGTGGTGCTGGGCGTCACTGCAATCAAAGACCTG GTGGACGATGTGGCTCGCCATAAAATGGATAAGGAAATCAACAATAGGACGTGTGAAGTC ATTAAGGATGGCAGGTTCAAAGTTGCTAAGTGGAAAGAAATTCAAGTTGGAGACGTCATT CGTCTGAAAAAAAATGATTTTGTTCCAGCTGACATTCTCCTGCTGTCTAGCTCTGAGCCT AACAGCCTCTGCTATGTGGAAACAGCAGAACTGGACGGAGAAACCAATTTAAAATTTAAG ATGTCACTTGAAATCACAGACCAGTACCTCCAAAGAGAAGATACATTGGCTACATTTGAT GGTTTTATTGAATGTGAAGAACCCAATAACCGACTAGATAAGTTTACAGGAACACTATTT TGGAGAAACACAAGTTTTCCTTTGGATGCTGATAAAATTTTGTTACGTGGCTGTGTAATT AGGAACACCGATTTCTGCCACGGCTTAGTCATTTTTGCAGGTGCTGACACTAAAATAATG AAGAATAGTGGGAAAACCAGATTTAAAAGAACTAAAATTGATTACTTGATGAACTACATG GTTTACACGATCTTTGTTGTTCTTATTCTGCTTTCTGCTGGTCTTGCCATCGGCCATGCT TATTGGGAAGCACAGGTGGGCAATTCCTCTTGGTACCTCTATGATGGAGAAGACGATACA CCCTCCTACCGTGGATTCCTCATTTTCTGGGGCTATATCATTGTTCTCAACACCATGGTA CCCATCTCTCTCTATGTCAGCGTGGAAGTGATTCGTCTTGGACAGAGTCACTTCATCAAC TGGGACCTGCAAATGTACTATGCTGAGAAGGACACACCCGCAAAAGCTAGAACCACCACA CTCAATGAACAGCTCGGGCAGATCCATTATATCTTCTCTGATAAGACGGGGACACTCACA CAAAATATCATGACCTTTAAAAAGTGCTGTATCAACGGGCAGATATATGGGGACCATCGG GATGCCTCTCAACACAACCACAACAAAATAGAGCAAGTTGATTTTAGCTGGAATACATAT GCTGATGGGAAGCTTGCATTTTATGACCACTATCTTATTGAGCAAATCCAGTCAGGGAAA GAGCCAGAAGTACGACAGTTCTTCTTCTTGCTCGCAGTTTGCCACACAGTCATGGTGGAT AGGACTGATGGTCAGCTCAACTACCAGGCAGCCTCTCCCGATGAAGGTGCCCTGGTAAAC GCTGCCAGGAACTTTGGCTTTGCCTTCCTCGCCAGGACCCAGAACACCATCACCATCAGT GAACTGGGCACTGAAAGGACTTACAATGTTCTTGCCATTTTGGACTTCAACAGTGACCGG AAGCGAATGTCTATCATTGTAAGAACCCCAGAAGGCAATATCAAGCTTTACTGTAAAGGT GCTGACACTGTTATTTATGAACGGTTACATCGAATGAATCCTACTAAGCAAGAAACACAG GATGCCCTGGATATCTTTGCAAATGAAACTCTTAGAACCCTATGCCTTTGCTACAAGGAA ATTGAAGAAAAAGAATTTACAGAATGGAATAAAAAGTTTATGGCTGCCAGTGTGGCCTCC ACCAACCGGGACGAAGCTCTGGATAAAGTATATGAGGAGATTGAAAAAGACTTAATTCTC CTGGGAGCTACAGCTATTGAAGACAAGCTACAGGATGGAGTTCCAGAAACCATTTCAAAA CTTGCAAAAGCTGACATTAAGATCTGGGTGCTTACTGGAGACAAAAAGGAAACTGCTGAA AATATAGGATTTGCTTGTGAACTTCTGACTGAAGACACCACCATCTGCTATGGGGAGGAT ATTAATTCTCTTCTTCATGCAAGGATGGAAAACCAGAGGAATAGAGGTGGCGTCTACGCA AAGTTTGCACCTCCTGTGCAGGAATCTTTTTTTCCACCCGGTGGAAACCGTGCCTTAATC ATCACTGGTTCTTGGTTGAATGAAATTCTTCTCGAGAAAAAGACCAAGAGAAATAAGATT CTGAAGCTGAAGTTCCCAAGAACAGAAGAAGAAAGACGGATGCGGACCCAAAGTAAAAGG AGGCTAGAAGCTAAGAAAGAGCAGCGGCAGAAAAACTTTGTGGACCTGGCCTGCGAGTGC AGCGCAGTCATCTGCTGCCGCGTCACCCCCAAGCAGAAGGCCATGGTGGTGGACCTGGTG AAGAGGTACAAGAAAGCCATCACGCTGGCCATCGGAGATGGGGCCAATGACGTGAACATG ATCAAAACTGCCCACATTGGCGTTGGAATAAGTGGACAAGAAGGAATGCAAGCTGTCATG TCGAGTGACTATTCCTTTGCTCAGTTCCGATATCTGCAGAGGCTACTGCTGGTGCATGGC CGATGGTCTTACATAAGGATGTGCAAGTTCCTACGATACTTCTTTTACAAAAACTTTGCC TTTACTTTGGTTCATTTCTGGTACTCCTTCTTCAATGGCTACTCTGCGCAGACTGCATAC GAGGATTGGTTCATCACCCTCTACAACGTGCTGTACACCAGCCTGCCCGTGCTCCTCATG GGGCTGCTCGACCAGGATGTGAGTGACAAACTGAGCCTCCGATTCCCTGGGTTATACATA GTGGGACAAAGAGACTTACTATTCAACTATAAGAGATTCTTTGTAAGCTTGTTGCATGGG GTCCTAACATCGATGATCCTCTTCTTCATACCTCTTGGAGCTTATCTGCAAACCGTAGGG CAGGATGGAGAGGCACCTTCCGACTACCAGTCTTTTGCCGTCACCATTGCCTCTGCTCTT GTAATAACAGTCAATTTCCAGATTGGCTTGGATACTTCTTATTGGACTTTTGTGAATGCT TTTTCAATTTTTGGAAGCATTGCACTTTATTTTGGCATCATGTTTGACTTTCATAGTGCT GGAATACATGTTCTCTTTCCATCTGCATTTCAATTTACAGGCACAGCTTCAAACGCTCTG AGACAGCCATACATTTGGTTAACTATCATCCTGACTGTTGCTGTGTGCTTACTACCCGTC GTTGCCATTCGATTCCTGTCAATGACCATCTGGCCATCAGAAAGTGATAAGATCCAGAAG CATCGCAAGCGGTTGAAGGCGGAGGAGCAGTGGCAGCGACGGCAGCAGGTGTTCCGCCGG GGCGTGTCAACGCGGCGCTCGGCCTACGCCTTCTCGCACCAGCGGGGCTACGCGGACCTC ATCTCCTCCGGGCGCAGCATCCGCAAGAAGCGCTCGCCGCTTGATGCCATCGTGGCGGAT GGCACCGCGGAGTACAGGCGCACCGGGGACAGC
Cloned ORF protein sequence for pF1KE2523 Download>KIEE2523 1251 aa MSTERDSETTFDEDSQPNDEVVPYSDDETEDELDDQGSAVEPEQNRVNREAEENREPFRK ECTWQVKANDRKYHEQPHFMNTKFLCIKESKYANNAIKTYKYNAFTFIPMNLFEQFKRAA NLYFLALLILQAVPQISTLAWYTTLVPLLVVLGVTAIKDLVDDVARHKMDKEINNRTCEV IKDGRFKVAKWKEIQVGDVIRLKKNDFVPADILLLSSSEPNSLCYVETAELDGETNLKFK MSLEITDQYLQREDTLATFDGFIECEEPNNRLDKFTGTLFWRNTSFPLDADKILLRGCVI RNTDFCHGLVIFAGADTKIMKNSGKTRFKRTKIDYLMNYMVYTIFVVLILLSAGLAIGHA YWEAQVGNSSWYLYDGEDDTPSYRGFLIFWGYIIVLNTMVPISLYVSVEVIRLGQSHFIN WDLQMYYAEKDTPAKARTTTLNEQLGQIHYIFSDKTGTLTQNIMTFKKCCINGQIYGDHR DASQHNHNKIEQVDFSWNTYADGKLAFYDHYLIEQIQSGKEPEVRQFFFLLAVCHTVMVD RTDGQLNYQAASPDEGALVNAARNFGFAFLARTQNTITISELGTERTYNVLAILDFNSDR KRMSIIVRTPEGNIKLYCKGADTVIYERLHRMNPTKQETQDALDIFANETLRTLCLCYKE IEEKEFTEWNKKFMAASVASTNRDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISK LAKADIKIWVLTGDKKETAENIGFACELLTEDTTICYGEDINSLLHARMENQRNRGGVYA KFAPPVQESFFPPGGNRALIITGSWLNEILLEKKTKRNKILKLKFPRTEEERRMRTQSKR RLEAKKEQRQKNFVDLACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNM IKTAHIGVGISGQEGMQAVMSSDYSFAQFRYLQRLLLVHGRWSYIRMCKFLRYFFYKNFA FTLVHFWYSFFNGYSAQTAYEDWFITLYNVLYTSLPVLLMGLLDQDVSDKLSLRFPGLYI VGQRDLLFNYKRFFVSLLHGVLTSMILFFIPLGAYLQTVGQDGEAPSDYQSFAVTIASAL VITVNFQIGLDTSYWTFVNAFSIFGSIALYFGIMFDFHSAGIHVLFPSAFQFTGTASNAL RQPYIWLTIILTVAVCLLPVVAIRFLSMTIWPSESDKIQKHRKRLKAEEQWQRRQQVFRR GVSTRRSAYAFSHQRGYADLISSGRSIRKKRSPLDAIVADGTAEYRRTGDS
Nucleotide Sequence (with vector) for pF1KE2523 Download>pF1KE2523 6866 bp GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGAGTACAGAA AGAGACTCAGAAACGACATTTGACGAGGATTCTCAGCCTAATGACGAAGTGGTTCCCTAC AGTGATGATGAAACAGAAGATGAACTTGATGACCAGGGGTCTGCTGTTGAACCAGAACAA AACCGAGTCAACAGGGAAGCAGAGGAGAACCGGGAGCCATTCAGAAAAGAATGTACATGG CAAGTCAAAGCAAACGATCGCAAGTACCACGAACAACCTCACTTTATGAACACAAAATTC TTGTGTATTAAGGAGAGTAAATATGCGAATAATGCAATTAAAACATACAAGTACAACGCA TTTACCTTTATACCAATGAATCTGTTTGAGCAGTTTAAGAGAGCAGCCAATTTATATTTC CTGGCTCTTCTTATCTTACAGGCAGTTCCTCAAATCTCTACCCTGGCTTGGTACACCACA CTAGTGCCCCTGCTTGTGGTGCTGGGCGTCACTGCAATCAAAGACCTGGTGGACGATGTG GCTCGCCATAAAATGGATAAGGAAATCAACAATAGGACGTGTGAAGTCATTAAGGATGGC AGGTTCAAAGTTGCTAAGTGGAAAGAAATTCAAGTTGGAGACGTCATTCGTCTGAAAAAA AATGATTTTGTTCCAGCTGACATTCTCCTGCTGTCTAGCTCTGAGCCTAACAGCCTCTGC TATGTGGAAACAGCAGAACTGGACGGAGAAACCAATTTAAAATTTAAGATGTCACTTGAA ATCACAGACCAGTACCTCCAAAGAGAAGATACATTGGCTACATTTGATGGTTTTATTGAA TGTGAAGAACCCAATAACCGACTAGATAAGTTTACAGGAACACTATTTTGGAGAAACACA AGTTTTCCTTTGGATGCTGATAAAATTTTGTTACGTGGCTGTGTAATTAGGAACACCGAT TTCTGCCACGGCTTAGTCATTTTTGCAGGTGCTGACACTAAAATAATGAAGAATAGTGGG AAAACCAGATTTAAAAGAACTAAAATTGATTACTTGATGAACTACATGGTTTACACGATC TTTGTTGTTCTTATTCTGCTTTCTGCTGGTCTTGCCATCGGCCATGCTTATTGGGAAGCA CAGGTGGGCAATTCCTCTTGGTACCTCTATGATGGAGAAGACGATACACCCTCCTACCGT GGATTCCTCATTTTCTGGGGCTATATCATTGTTCTCAACACCATGGTACCCATCTCTCTC TATGTCAGCGTGGAAGTGATTCGTCTTGGACAGAGTCACTTCATCAACTGGGACCTGCAA ATGTACTATGCTGAGAAGGACACACCCGCAAAAGCTAGAACCACCACACTCAATGAACAG CTCGGGCAGATCCATTATATCTTCTCTGATAAGACGGGGACACTCACACAAAATATCATG ACCTTTAAAAAGTGCTGTATCAACGGGCAGATATATGGGGACCATCGGGATGCCTCTCAA CACAACCACAACAAAATAGAGCAAGTTGATTTTAGCTGGAATACATATGCTGATGGGAAG CTTGCATTTTATGACCACTATCTTATTGAGCAAATCCAGTCAGGGAAAGAGCCAGAAGTA CGACAGTTCTTCTTCTTGCTCGCAGTTTGCCACACAGTCATGGTGGATAGGACTGATGGT CAGCTCAACTACCAGGCAGCCTCTCCCGATGAAGGTGCCCTGGTAAACGCTGCCAGGAAC TTTGGCTTTGCCTTCCTCGCCAGGACCCAGAACACCATCACCATCAGTGAACTGGGCACT GAAAGGACTTACAATGTTCTTGCCATTTTGGACTTCAACAGTGACCGGAAGCGAATGTCT ATCATTGTAAGAACCCCAGAAGGCAATATCAAGCTTTACTGTAAAGGTGCTGACACTGTT ATTTATGAACGGTTACATCGAATGAATCCTACTAAGCAAGAAACACAGGATGCCCTGGAT ATCTTTGCAAATGAAACTCTTAGAACCCTATGCCTTTGCTACAAGGAAATTGAAGAAAAA GAATTTACAGAATGGAATAAAAAGTTTATGGCTGCCAGTGTGGCCTCCACCAACCGGGAC GAAGCTCTGGATAAAGTATATGAGGAGATTGAAAAAGACTTAATTCTCCTGGGAGCTACA GCTATTGAAGACAAGCTACAGGATGGAGTTCCAGAAACCATTTCAAAACTTGCAAAAGCT GACATTAAGATCTGGGTGCTTACTGGAGACAAAAAGGAAACTGCTGAAAATATAGGATTT GCTTGTGAACTTCTGACTGAAGACACCACCATCTGCTATGGGGAGGATATTAATTCTCTT CTTCATGCAAGGATGGAAAACCAGAGGAATAGAGGTGGCGTCTACGCAAAGTTTGCACCT CCTGTGCAGGAATCTTTTTTTCCACCCGGTGGAAACCGTGCCTTAATCATCACTGGTTCT TGGTTGAATGAAATTCTTCTCGAGAAAAAGACCAAGAGAAATAAGATTCTGAAGCTGAAG TTCCCAAGAACAGAAGAAGAAAGACGGATGCGGACCCAAAGTAAAAGGAGGCTAGAAGCT AAGAAAGAGCAGCGGCAGAAAAACTTTGTGGACCTGGCCTGCGAGTGCAGCGCAGTCATC TGCTGCCGCGTCACCCCCAAGCAGAAGGCCATGGTGGTGGACCTGGTGAAGAGGTACAAG AAAGCCATCACGCTGGCCATCGGAGATGGGGCCAATGACGTGAACATGATCAAAACTGCC CACATTGGCGTTGGAATAAGTGGACAAGAAGGAATGCAAGCTGTCATGTCGAGTGACTAT TCCTTTGCTCAGTTCCGATATCTGCAGAGGCTACTGCTGGTGCATGGCCGATGGTCTTAC ATAAGGATGTGCAAGTTCCTACGATACTTCTTTTACAAAAACTTTGCCTTTACTTTGGTT CATTTCTGGTACTCCTTCTTCAATGGCTACTCTGCGCAGACTGCATACGAGGATTGGTTC ATCACCCTCTACAACGTGCTGTACACCAGCCTGCCCGTGCTCCTCATGGGGCTGCTCGAC CAGGATGTGAGTGACAAACTGAGCCTCCGATTCCCTGGGTTATACATAGTGGGACAAAGA GACTTACTATTCAACTATAAGAGATTCTTTGTAAGCTTGTTGCATGGGGTCCTAACATCG ATGATCCTCTTCTTCATACCTCTTGGAGCTTATCTGCAAACCGTAGGGCAGGATGGAGAG GCACCTTCCGACTACCAGTCTTTTGCCGTCACCATTGCCTCTGCTCTTGTAATAACAGTC AATTTCCAGATTGGCTTGGATACTTCTTATTGGACTTTTGTGAATGCTTTTTCAATTTTT GGAAGCATTGCACTTTATTTTGGCATCATGTTTGACTTTCATAGTGCTGGAATACATGTT CTCTTTCCATCTGCATTTCAATTTACAGGCACAGCTTCAAACGCTCTGAGACAGCCATAC ATTTGGTTAACTATCATCCTGACTGTTGCTGTGTGCTTACTACCCGTCGTTGCCATTCGA TTCCTGTCAATGACCATCTGGCCATCAGAAAGTGATAAGATCCAGAAGCATCGCAAGCGG TTGAAGGCGGAGGAGCAGTGGCAGCGACGGCAGCAGGTGTTCCGCCGGGGCGTGTCAACG CGGCGCTCGGCCTACGCCTTCTCGCACCAGCGGGGCTACGCGGACCTCATCTCCTCCGGG CGCAGCATCCGCAAGAAGCGCTCGCCGCTTGATGCCATCGTGGCGGATGGCACCGCGGAG TACAGGCGCACCGGGGACAGCGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTA GAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGA GTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGT CTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAAC CGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCT TGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTT CTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTT GCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAG CCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCA AGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCAC GCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACA ATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTT GTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCG TGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGA AGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCT CCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCG GCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATG GAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCC GAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCAT GGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGAC TGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATT GCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCT CCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTC TGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGT AATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCA GCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC CCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACT ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCT GCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAG CTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCA CGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAA CCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGC GAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAG AAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCA GCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTC TGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG GATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTT CGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTG GCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCG GCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAAC GCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCG TAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGA TCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTT GCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACG CCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATC AAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGG TGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAC GGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGA AGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAA ATACATTCAAATATGTATCCGCTCAT
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