C17orf77 ORF clone pF1KB7423

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Flexi ORF Clone pF1KB7423

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Clone info.

Species

human

Clone Name

pF1KB7423 Sequence (with vector) Restriction Map

Product ID

FXC02450

HaloTag Clone PID

FHC02450

ORF Sequence

DNA sequence (729 bp)
Protein sequence (243 aa)

Linker Type

Flexi type

more Linker info

The extra Val codon is attached to the 3'-end of the ORF, because a Pme I site (GTTTAAAC) is tagged at the 3' end of the ORF instead of the stop codon. The resultant sequence encodes Val-stop in place of stop. By cutting the sequence using Pme I, the stop codon arising in the Pme I site can be omitted.

Vector Type

pF1K Vector sequence Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB463510]

Gene

KIBB7423

Symbol

C17orf77

Description

chromosome 17 open reading frame 77, transcript variant 2

Original Clone ID

oc00637

Features of the cloned ORF sequence

Description

Length: 729 bp

Restriction map A Restriction map B

Genomic Structure

Features of the protein sequence

Description

Length: 243 aa

Result of homology search against CCDS protein database ( FASTA output, Multiple alignment)

no hit
Inquiries or Suggestions ?
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ORF DNA

Cloned ORF Nucleotide sequence for pF1KB7423 Download

>KIBB7423  729 bp
ATGGATGAATTGGCGCTGTCATTTTCCCTGACATGTCTCCTTCCTGAGAACAGAGCGTCT
CTGTCTCCAAGCCAGCCGCTGTCATTCCAGTGCCTTAAAGCTCCAGCCACACTCACCTGG
GAGGATGAGAAGCAGCAGAGATGGGGACAGCCACATGGTCCTGTCTCCTCTCCTCTCCTT
GGTGATCACAGGTGTCTGGTGCCCTTCAGGGACTTGAATCCAAGCTCGGAAGTCAACACC
GCAAACCTACTCGAATCTCCTTCAAGTCTCTTGCTCACTTCCTGTTACATCTGTAGCTAC
TTCTCTTTTTACATATTAGGAGAAAAGAGGTGTCATTCTTTGAAAAGACTAAGGTACAGT
GTATGTTGCAAGGTGTGCCCAAACTTTTGTGCATGTGGAAAAGAAAATGTGTCCGGGACT
GGTCAGGTTTGCACGGGAGTGCACGTAGGAGCCAAGGAGCAGGAGGAGCCAGGAGGAACC
CAAGCATTGAGGAGCTGCGGGATCTACTGCCTGGAGGAGAGGACGGACAAGGCCAGCCAT
GAGGAGTGCAGGGAGAGGAGCACTTTGGGAAGACCACAGTGCACAGGACTCACACCCAGC
CTTGCTGGAGAAAGCCCATGCCCCAGGCTTCTTCCAGGCAGCCCCACTGTAAGACATCTC
ATTGCCTCTTCCTGCCCTGGGCTGTCTGACCCACTGCCCCTTCCCCCAGGAACACTTCCA
CTGGGATCA

ORF Protein

Cloned ORF protein sequence for pF1KB7423 Download

>KIBB7423  243 aa
MDELALSFSLTCLLPENRASLSPSQPLSFQCLKAPATLTWEDEKQQRWGQPHGPVSSPLL
GDHRCLVPFRDLNPSSEVNTANLLESPSSLLLTSCYICSYFSFYILGEKRCHSLKRLRYS
VCCKVCPNFCACGKENVSGTGQVCTGVHVGAKEQEEPGGTQALRSCGIYCLEERTDKASH
EECRERSTLGRPQCTGLTPSLAGESPCPRLLPGSPTVRHLIASSCPGLSDPLPLPPGTLP
LGS

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB7423 Download

>pF1KB7423  3842 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGATGAATTG
GCGCTGTCATTTTCCCTGACATGTCTCCTTCCTGAGAACAGAGCGTCTCTGTCTCCAAGC
CAGCCGCTGTCATTCCAGTGCCTTAAAGCTCCAGCCACACTCACCTGGGAGGATGAGAAG
CAGCAGAGATGGGGACAGCCACATGGTCCTGTCTCCTCTCCTCTCCTTGGTGATCACAGG
TGTCTGGTGCCCTTCAGGGACTTGAATCCAAGCTCGGAAGTCAACACCGCAAACCTACTC
GAATCTCCTTCAAGTCTCTTGCTCACTTCCTGTTACATCTGTAGCTACTTCTCTTTTTAC
ATATTAGGAGAAAAGAGGTGTCATTCTTTGAAAAGACTAAGGTACAGTGTATGTTGCAAG
GTGTGCCCAAACTTTTGTGCATGTGGAAAAGAAAATGTGTCCGGGACTGGTCAGGTTTGC
ACGGGAGTGCACGTAGGAGCCAAGGAGCAGGAGGAGCCAGGAGGAACCCAAGCATTGAGG
AGCTGCGGGATCTACTGCCTGGAGGAGAGGACGGACAAGGCCAGCCATGAGGAGTGCAGG
GAGAGGAGCACTTTGGGAAGACCACAGTGCACAGGACTCACACCCAGCCTTGCTGGAGAA
AGCCCATGCCCCAGGCTTCTTCCAGGCAGCCCCACTGTAAGACATCTCATTGCCTCTTCC
TGCCCTGGGCTGTCTGACCCACTGCCCCTTCCCCCAGGAACACTTCCACTGGGATCAGTT
TAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGC
TGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCA
ATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGG
AGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGT
AAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGC
CCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTT
CCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAA
TTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCT
TTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGA
CGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTG
GAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTG
TTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCC
CTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCT
TGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAA
GTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATG
GCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAA
GCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGAT
GATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCG
CGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATC
ATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGAC
CGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGG
GCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTC
TATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAG
CGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGG
GGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAA
GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG
ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC
TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC
CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC
GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG
CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCC
ACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGA
GTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGC
TCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAAC
CACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGG
ATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTC
ACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATA
GTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAA
AATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGG
TATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGC
CATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTA
AGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTA
CTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAA
ATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGT
CCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGG
GTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGA
AAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAA
ATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAC
GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTT
TTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
AT