RASGRP4 ORF clone FHC30817

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Halotag ORF Clone FHC30817

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Clone info.

Species

human

Clone Name

pFN21AE3270 Sequence (with vector) Restriction Map

Product ID

FHC30817

ORF Sequence

DNA sequence (2019 bp)
Protein sequence (673 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3270

Symbol

RASGRP4

Description

RAS guanyl releasing protein 4, transcript variant a

Original Clone ID

cp93270

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2019 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 673 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46068.1 0 100.0 RASGRP4 CCDS59382.1 1.4e-168 97.9 RASGRP4 CCDS59383.1 9.8e-145 94.9 RASGRP4 CCDS54263.1 2.5e-123 85.6 RASGRP4 CCDS54264.1 1.1e-105 86.3 RASGRP4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020454 538 552 PR00008 Diacylglycerol/phorbol-ester binding IPR020454 554 563 PR00008 Diacylglycerol/phorbol-ester binding IPR020454 567 578 PR00008 Diacylglycerol/phorbol-ester binding IPR020454 579 591 PR00008 Diacylglycerol/phorbol-ester binding HMMPfam IPR001895 200 373 PF00617 Guanine-nucleotide dissociation stimulator CDC25 IPR002219 541 592 PF00130 Protein kinase C-like HMMSmart IPR001895 197 433 SM00147 Guanine-nucleotide dissociation stimulator CDC25 IPR002219 541 590 SM00109 Protein kinase C-like ProfileScan IPR000651 49 175 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 201 432 PS50009 Guanine-nucleotide dissociation stimulator CDC25 IPR018249 466 501 PS50222 EF-HAND 2 IPR002219 540 590 PS50081 Protein kinase C-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_733749 673 607320 100.0 100.0 NP_001139674 659 607320 97.9 100.0 NP_001139677 639 607320 94.9 100.0 NP_001139679 576 607320 85.6 100.0 NP_001139675 581 607320 86.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3270 Download

>KIEE3270  2019 bp
ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAATAGGAGGG
CGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGC
AAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAG
CTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGCTGGCAGCCTGTGCCACGAGGACCAC
ATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGCCGTCCGCCGACCTGGCTGCC
CGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAGACGGCTGCAG
ATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC
CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCC
CAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTC
CCAATGAGCAGCCCAGGCCTGGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTG
GAGACGGGGGAGCTGGCTCAGCACCTCACCTACCTGGAGTTCCGGTCCTTCCAGGCTATC
ACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAGTACGAGGCTGCCCGGCCCTG
GAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTGCAGGTGATGGTGCTGAGC
CGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTCATTCACGTGGCACAGAGG
CTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACAGGGGGCCTGTGTCACAGT
GCCATCTCCAGACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCAAGGCCCTC
CTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAACTACGCCCGCTACCGCCGCACCTGG
GCTGGCTGCGCGGGCTTCCGGCTGCCTGTACTGGGCGTGCACCTCAAGGACCTGGTGTCC
CTGCATGAGGCACAGCCCGACAGGTTGCCTGACGGCCGCCTGCACCTACCCAAGCTGAAC
AACCTCTACCTGCGGCTGCAGGAGCTGGTGGCCCTCCAAGGGCAGCATCCACCCTGCAGC
GCCAATGAGGATCTGCTGCACCTGCTCACGCTCTCCCTGGACCTCTTCTACACGGAAGAC
GAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCC
CCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGG
GTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCT
GAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGAGCGACTCTCGGGCAATTTTCCCTTC
GCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGGAGAGGATCCTTCAGCAGAGAGGAG
CTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGGGCCTGGCCTTCCTG
CACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGGCTTC
CTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAA
CACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCA
GGACCCCCCGGAGCTCCTGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAG
GAAAATCACTCCTACACGCTATCCCTGGAGCCTGAGACTGGGTGCCAGCTTCGCCATGCC
TGGACCCAGACTGAATCCCCACACCCTTCCTGGGAAACAGATACGGTCCCCTGCCCGGTG
ATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE3270 Download

>KIEE3270  673 aa
MNRKDSKRKSHQECTGKIGGRGRPRQVRRHKTCPSPREISKVMASMNLGLLSEGGCSEDE
LLEKCIQSFDSAGSLCHEDHMLNMVLAMHSWVLPSADLAARLLTSYQKATGDTQELRRLQ
ICHLVRYWLMRHPEVMHQDPQLEEVIGRFWATVAREGNSAQRRLGDSSDLLSPGGPGPPL
PMSSPGLGKKRKVSLLFDHLETGELAQHLTYLEFRSFQAITPQDLRSYVLQGSVRGCPAL
EGSVGLSNSVSRWVQVMVLSRPGPLQRAQVLDKFIHVAQRLHQLQNFNTLMAVTGGLCHS
AISRLKDSHAHLSPDSTKALLELTELLASHNNYARYRRTWAGCAGFRLPVLGVHLKDLVS
LHEAQPDRLPDGRLHLPKLNNLYLRLQELVALQGQHPPCSANEDLLHLLTLSLDLFYTED
EIYELSYAREPRCPKSLPPSPFNAPLVVEWAPGVTPKPDRVTLGRHVEQLVESVFKNYDP
EGRGTISQEDFERLSGNFPFACHGLHPPPRQGRGSFSREELTGYLLRASAICSKLGLAFL
HTFHEVTFRKPTFCDSCSGFLWGVTKQGYRCRECGLCCHKHCRDQVKVECKKRPGAKGDA
GPPGAPVPSTPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQTESPHPSWETDTVPCPV
MDPPSTASSKLDS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3270 Download

>pFN21AE3270  6724 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCA
GGAATGCACCGGAAAAATAGGAGGGCGAGGCCGGCCCCGCCAAGTGCGCCGCCACAAGAC
ATGCCCCAGCCCTCGGGAAATCAGCAAGGTCATGGCTTCCATGAACCTGGGCCTGCTGAG
TGAGGGCGGCTGCAGCGAAGATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGATTCAGC
TGGCAGCCTGTGCCACGAGGACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGT
GCTGCCGTCCGCCGACCTGGCTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGA
CACCCAGGAGCTGAGACGGCTGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACA
CCCTGAGGTGATGCACCAGGATCCCCAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCAC
CGTGGCCCGGGAGGGCAACTCAGCCCAGAGAAGACTGGGAGACTCTTCTGACCTCCTGAG
CCCTGGTGGCCCTGGCCCCCCACTCCCAATGAGCAGCCCAGGCCTGGGCAAAAAGCGCAA
AGTGTCCTTGCTTTTCGACCACTTGGAGACGGGGGAGCTGGCTCAGCACCTCACCTACCT
GGAGTTCCGGTCCTTCCAGGCTATCACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGG
CTCAGTACGAGGCTGCCCGGCCCTGGAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCG
CTGGGTGCAGGTGATGGTGCTGAGCCGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGA
CAAGTTCATTCACGTGGCACAGAGGCTCCACCAGCTGCAGAATTTCAACACGCTGATGGC
AGTCACAGGGGGCCTGTGTCACAGTGCCATCTCCAGACTCAAGGACTCCCATGCCCACCT
GAGCCCTGACAGCACCAAGGCCCTCCTGGAGCTCACTGAGCTCCTTGCCTCCCACAACAA
CTACGCCCGCTACCGCCGCACCTGGGCTGGCTGCGCGGGCTTCCGGCTGCCTGTACTGGG
CGTGCACCTCAAGGACCTGGTGTCCCTGCATGAGGCACAGCCCGACAGGTTGCCTGACGG
CCGCCTGCACCTACCCAAGCTGAACAACCTCTACCTGCGGCTGCAGGAGCTGGTGGCCCT
CCAAGGGCAGCATCCACCCTGCAGCGCCAATGAGGATCTGCTGCACCTGCTCACGCTCTC
CCTGGACCTCTTCTACACGGAAGACGAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCG
TTGTCCCAAGAGCCTGCCACCCTCCCCCTTCAATGCACCTCTGGTGGTGGAGTGGGCCCC
TGGTGTGACACCCAAGCCGGACAGGGTCACACTGGGTCGGCATGTGGAGCAGCTGGTGGA
GTCTGTGTTCAAGAATTATGACCCTGAAGGCCGAGGAACAATCTCTCAGGAGGACTTTGA
GCGACTCTCGGGCAATTTTCCCTTCGCCTGCCATGGGCTTCACCCACCCCCACGCCAGGG
GAGAGGATCCTTCAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTG
CTCCAAGTTGGGCCTGGCCTTCCTGCACACCTTCCATGAGGTCACCTTCCGAAAGCCTAC
CTTCTGCGACAGCTGCAGTGGCTTCCTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCG
GGAGTGCGGGCTGTGTTGCCACAAACACTGCAGAGACCAGGTGAAGGTAGAATGTAAGAA
GAGGCCAGGGGCCAAGGGCGATGCAGGACCCCCCGGAGCTCCTGTCCCATCCACACCAGC
TCCCCATGCCAGCTGTGGCTCCGAGGAAAATCACTCCTACACGCTATCCCTGGAGCCTGA
GACTGGGTGCCAGCTTCGCCATGCCTGGACCCAGACTGAATCCCCACACCCTTCCTGGGA
AACAGATACGGTCCCCTGCCCGGTGATGGACCCACCATCAACTGCATCCTCCAAGCTGGA
TTCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT