RASGRP2 ORF clone FHC28050

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC28050

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Clone info.

Species

human

Clone Name

pFN21AE2026 Sequence (with vector) Restriction Map

Product ID

FHC28050

ORF Sequence

DNA sequence (1827 bp)
Protein sequence (609 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590919]

Gene

KIEE2026

Symbol

RASGRP2

Alias : CALDAG-GEFI, CDC25L

Description

RAS guanyl releasing protein 2 (calcium and DAG-regulated), transcript variant 2

Original Clone ID

cs02926

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1827 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 609 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31598.1 1.4e-205 100.0 RASGRP2 CCDS54346.1 1.4e-99 52.7 RASGRP3 CCDS46256.1 1.7e-99 53.0 RASGRP3 CCDS45222.1 9.3e-80 46.5 RASGRP1 CCDS46068.1 5.5e-77 44.5 RASGRP4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000651 9 102 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 153 324 PF00617 Guanine-nucleotide dissociation stimulator CDC25 IPR002219 499 550 PF00130 Protein kinase C-like HMMSmart IPR000651 3 126 SM00229 Ras-like guanine nucleotide exchange factor IPR001895 150 388 SM00147 Guanine-nucleotide dissociation stimulator CDC25 IPR002048 430 458 SM00054 Calcium-binding EF-hand IPR002048 459 487 SM00054 Calcium-binding EF-hand IPR002219 499 548 SM00109 Protein kinase C-like ProfileScan IPR000651 4 126 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 154 387 PS50009 Guanine-nucleotide dissociation stimulator CDC25 IPR018249 426 461 PS50222 EF-HAND 2 IPR018249 463 490 PS50222 EF-HAND 2 IPR002219 498 548 PS50081 Protein kinase C-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_722541 609 605577,615888 100.0 100.0 NP_001092141 609 605577,615888 100.0 100.0 NP_001092140 609 605577,615888 100.0 100.0 XP_011543023 610 605577,615888 99.8 100.0 XP_016872573 610 605577,615888 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2026 Download

>KIEE2026  1827 bp
ATGGCAGGCACCCTGGACCTGGACAAGGGCTGCACGGTGGAGGAGCTGCTCCGCGGGTGC
ATCGAAGCCTTCGATGACTCCGGGAAGGTGCGGGACCCGCAGCTGGTGCGCATGTTCCTC
ATGATGCACCCCTGGTACATCCCCTCCTCTCAGCTGGCGGCCAAGCTGCTCCACATCTAC
CAACAATCCCGGAAGGACAACTCCAATTCCCTGCAGGTGAAAACGTGCCACCTGGTCAGG
TACTGGATCTCCGCCTTCCCAGCGGAGTTTGACTTGAACCCGGAGTTGGCTGAGCAGATC
AAGGAGCTGAAGGCTCTGCTAGACCAAGAAGGGAACCGACGGCACAGCAGCCTAATCGAC
ATAGACAGCGTCCCTACCTACAAGTGGAAGCGGCAGGTGACTCAGCGGAACCCTGTGGGA
CAGAAAAAGCGCAAGATGTCCCTGTTGTTTGACCACCTGGAGCCCATGGAGCTGGCGGAG
CATCTCACCTACTTGGAGTATCGCTCCTTCTGCAAGATCCTGTTTCAGGACTATCACAGT
TTCGTGACTCATGGCTGCACTGTGGACAACCCCGTCCTGGAGCGGTTCATCTCCCTCTTC
AACAGCGTCTCACAGTGGGTGCAGCTCATGATCCTCAGCAAACCCACAGCCCCGCAGCGG
GCCCTGGTCATCACACACTTTGTCCACGTGGCGGAGAAGCTGCTACAGCTGCAGAACTTC
AACACGCTGATGGCAGTGGTCGGGGGCCTGAGCCACAGCTCCATCTCCCGCCTCAAGGAG
ACCCACAGCCACGTTAGCCCTGAGACCATCAAGCTCTGGGAGGGTCTCACGGAACTAGTG
ACGGCGACAGGCAACTATGGCAACTACCGGCGTCGGCTGGCAGCCTGTGTGGGCTTCCGC
TTCCCGATCCTGGGTGTGCACCTCAAGGACCTGGTGGCCCTGCAGCTGGCACTGCCTGAC
TGGCTGGACCCAGCCCGGACCCGGCTCAACGGGGCCAAGATGAAGCAGCTCTTTAGCATC
CTGGAGGAGCTGGCCATGGTGACCAGCCTGCGGCCACCAGTACAGGCCAACCCCGACCTG
CTGAGCCTGCTCACGGTGTCTCTGGATCAGTATCAGACGGAGGATGAGCTGTACCAGCTG
TCCCTGCAGCGGGAGCCGCGCTCCAAGTCCTCGCCAACCAGCCCCACGAGTTGCACCCCA
CCACCCCGGCCCCCGGTACTGGAGGAGTGGACCTCGGCTGCCAAACCCAAGCTGGATCAG
GCCCTCGTGGTGGAGCACATCGAGAAGATGGTGGAGTCTGTGTTCCGGAACTTTGACGTC
GATGGGGATGGCCACATCTCACAGGAAGAATTCCAGATCATCCGTGGGAACTTCCCTTAC
CTCAGCGCCTTTGGGGACCTCGACCAGAACCAGGATGGCTGCATCAGCAGGGAGGAGATG
GTTTCCTATTTCCTGCGCTCCAGCTCTGTGTTGGGGGGGCGCATGGGCTTCGTACACAAC
TTCCAGGAGAGCAACTCCTTGCGCCCCGTCGCCTGCCGCCACTGCAAAGCCCTGATCCTG
GGCATCTACAAGCAGGGCCTCAAATGCCGAGCCTGTGGAGTGAACTGCCACAAGCAGTGC
AAGGATCGCCTGTCAGTTGAGTGTCGGCGCAGGGCCCAGAGTGTGAGCCTGGAGGGGTCT
GCACCCTCACCCTCACCCATGCACAGCCACCATCACCGCGCCTTCAGCTTCTCTCTGCCC
CGCCCTGGCAGGCGAGGCTCCAGGCCTCCAGAGATCCGTGAGGAGGAGGTACAGACGGTG
GAGGATGGGGTGTTTGACATCCACTTG

ORF Protein

Cloned ORF protein sequence for pFN21AE2026 Download

>KIEE2026  609 aa
MAGTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLAAKLLHIY
QQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAEQIKELKALLDQEGNRRHSSLID
IDSVPTYKWKRQVTQRNPVGQKKRKMSLLFDHLEPMELAEHLTYLEYRSFCKILFQDYHS
FVTHGCTVDNPVLERFISLFNSVSQWVQLMILSKPTAPQRALVITHFVHVAEKLLQLQNF
NTLMAVVGGLSHSSISRLKETHSHVSPETIKLWEGLTELVTATGNYGNYRRRLAACVGFR
FPILGVHLKDLVALQLALPDWLDPARTRLNGAKMKQLFSILEELAMVTSLRPPVQANPDL
LSLLTVSLDQYQTEDELYQLSLQREPRSKSSPTSPTSCTPPPRPPVLEEWTSAAKPKLDQ
ALVVEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSAFGDLDQNQDGCISREEM
VSYFLRSSSVLGGRMGFVHNFQESNSLRPVACRHCKALILGIYKQGLKCRACGVNCHKQC
KDRLSVECRRRAQSVSLEGSAPSPSPMHSHHHRAFSFSLPRPGRRGSRPPEIREEEVQTV
EDGVFDIHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2026 Download

>pFN21AE2026  6532 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGGCACCCTGGACCTGGACAAGGGCTGCAC
GGTGGAGGAGCTGCTCCGCGGGTGCATCGAAGCCTTCGATGACTCCGGGAAGGTGCGGGA
CCCGCAGCTGGTGCGCATGTTCCTCATGATGCACCCCTGGTACATCCCCTCCTCTCAGCT
GGCGGCCAAGCTGCTCCACATCTACCAACAATCCCGGAAGGACAACTCCAATTCCCTGCA
GGTGAAAACGTGCCACCTGGTCAGGTACTGGATCTCCGCCTTCCCAGCGGAGTTTGACTT
GAACCCGGAGTTGGCTGAGCAGATCAAGGAGCTGAAGGCTCTGCTAGACCAAGAAGGGAA
CCGACGGCACAGCAGCCTAATCGACATAGACAGCGTCCCTACCTACAAGTGGAAGCGGCA
GGTGACTCAGCGGAACCCTGTGGGACAGAAAAAGCGCAAGATGTCCCTGTTGTTTGACCA
CCTGGAGCCCATGGAGCTGGCGGAGCATCTCACCTACTTGGAGTATCGCTCCTTCTGCAA
GATCCTGTTTCAGGACTATCACAGTTTCGTGACTCATGGCTGCACTGTGGACAACCCCGT
CCTGGAGCGGTTCATCTCCCTCTTCAACAGCGTCTCACAGTGGGTGCAGCTCATGATCCT
CAGCAAACCCACAGCCCCGCAGCGGGCCCTGGTCATCACACACTTTGTCCACGTGGCGGA
GAAGCTGCTACAGCTGCAGAACTTCAACACGCTGATGGCAGTGGTCGGGGGCCTGAGCCA
CAGCTCCATCTCCCGCCTCAAGGAGACCCACAGCCACGTTAGCCCTGAGACCATCAAGCT
CTGGGAGGGTCTCACGGAACTAGTGACGGCGACAGGCAACTATGGCAACTACCGGCGTCG
GCTGGCAGCCTGTGTGGGCTTCCGCTTCCCGATCCTGGGTGTGCACCTCAAGGACCTGGT
GGCCCTGCAGCTGGCACTGCCTGACTGGCTGGACCCAGCCCGGACCCGGCTCAACGGGGC
CAAGATGAAGCAGCTCTTTAGCATCCTGGAGGAGCTGGCCATGGTGACCAGCCTGCGGCC
ACCAGTACAGGCCAACCCCGACCTGCTGAGCCTGCTCACGGTGTCTCTGGATCAGTATCA
GACGGAGGATGAGCTGTACCAGCTGTCCCTGCAGCGGGAGCCGCGCTCCAAGTCCTCGCC
AACCAGCCCCACGAGTTGCACCCCACCACCCCGGCCCCCGGTACTGGAGGAGTGGACCTC
GGCTGCCAAACCCAAGCTGGATCAGGCCCTCGTGGTGGAGCACATCGAGAAGATGGTGGA
GTCTGTGTTCCGGAACTTTGACGTCGATGGGGATGGCCACATCTCACAGGAAGAATTCCA
GATCATCCGTGGGAACTTCCCTTACCTCAGCGCCTTTGGGGACCTCGACCAGAACCAGGA
TGGCTGCATCAGCAGGGAGGAGATGGTTTCCTATTTCCTGCGCTCCAGCTCTGTGTTGGG
GGGGCGCATGGGCTTCGTACACAACTTCCAGGAGAGCAACTCCTTGCGCCCCGTCGCCTG
CCGCCACTGCAAAGCCCTGATCCTGGGCATCTACAAGCAGGGCCTCAAATGCCGAGCCTG
TGGAGTGAACTGCCACAAGCAGTGCAAGGATCGCCTGTCAGTTGAGTGTCGGCGCAGGGC
CCAGAGTGTGAGCCTGGAGGGGTCTGCACCCTCACCCTCACCCATGCACAGCCACCATCA
CCGCGCCTTCAGCTTCTCTCTGCCCCGCCCTGGCAGGCGAGGCTCCAGGCCTCCAGAGAT
CCGTGAGGAGGAGGTACAGACGGTGGAGGATGGGGTGTTTGACATCCACTTGGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT