GPR52 ORF clone FHC27732

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC27732

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Clone info.

Species

human

Clone Name

pFN21AE9414 Sequence (with vector) Restriction Map

Product ID

FHC27732

ORF Sequence

DNA sequence (1083 bp)
Protein sequence (361 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9414

Symbol

GPR52

Description

G protein-coupled receptor 52

Original Clone ID

cp99414

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1083 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 361 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30941.1 1.2e-96 100.0 GPR52 CCDS6849.1 2.4e-60 67.5 GPR21 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 42 66 PR00237 GPCR IPR000276 76 97 PR00237 GPCR IPR000276 121 143 PR00237 GPCR IPR000276 157 178 PR00237 GPCR IPR000276 263 287 PR00237 GPCR IPR000276 299 325 PR00237 GPCR HMMPfam IPR000276 57 317 PF00001 GPCR ProfileScan IPR017452 57 317 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 42 FETVVIVLLTFLIIAGNLTVIFV 64 PRIMARY 23 2 86 ADLFVGVSCLVPTLSLLHYSTGV 108 SECONDARY 23 3 120 IISVLKSVSMACLACISVDRYLA 142 SECONDARY 23 4 159 RICIILIWIYSCLIFLPSFFGWG 181 PRIMARY 23 5 205 GFIVCLLYAPAAFVVCFTYFHIF 227 PRIMARY 23 6 268 FRITSVFYMLWLPYIIYFLLESS 290 SECONDARY 23 7 300 FLTTWLAISNSFCNCVIYSLSNS 322 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005675 361 604106 100.0 100.0 NP_005285 349 601909 67.5 94.7 XP_005251990 349 601909 67.5 94.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9414 Download

>KIEE9414  1083 bp
ATGAATGAATCCAGGTGGACTGAATGGAGGATCCTGAACATGAGCAGTGGCATTGTGAAT
GTGTCCGAGCGTCACTCCTGCCCACTTGGATTTGGCCACTACAGTGTGGTGGATGTCTGC
ATCTTCGAGACAGTGGTTATTGTGTTGCTGACATTTCTGATCATTGCTGGGAATCTAACA
GTTATCTTTGTCTTTCATTGTGCTCCACTGTTACATCATTATACTACCAGCTATTTCATT
CAGACGATGGCATATGCTGATCTTTTCGTTGGAGTTAGCTGCTTGGTTCCTACTCTGTCA
CTTCTCCACTACTCCACAGGTGTCCACGAGTCATTGACTTGCCAGGTTTTTGGATATATC
ATCTCAGTTCTAAAAAGTGTTTCTATGGCATGTCTTGCTTGCATCAGTGTGGATCGTTAT
CTTGCAATAACCAAGCCTCTTTCCTACAATCAACTGGTCACCCCTTGTCGCTTGAGAATT
TGCATTATTTTGATCTGGATCTACTCCTGCCTAATTTTCTTGCCTTCCTTTTTTGGCTGG
GGGAAACCTGGTTACCATGGTGACATTTTTGAATGGTGTGCCACGTCTTGGCTCACCAGT
GCCTATTTTACTGGCTTTATTGTTTGTTTACTTTATGCTCCTGCTGCCTTTGTTGTCTGC
TTCACTTACTTCCACATTTTCAAAATTTGCCGTCAGCACACCAAAGAGATAAATGACCGA
AGAGCCCGATTCCCTAGTCATGAGGTAGATTCTTCCAGAGAGACTGGACACAGCCCTGAC
CGTCGCTACGCCATGGTTTTGTTTAGGATAACCAGTGTATTTTATATGCTGTGGCTCCCC
TATATAATTTACTTTCTTCTAGAAAGCTCCCGGGTCTTGGACAATCCAACTCTGTCCTTC
TTAACAACCTGGCTTGCAATAAGTAATAGTTTTTGTAACTGTGTAATATACAGCCTCTCC
AACAGCGTTTTCCGGCTAGGCCTCCGAAGACTGTCTGAGACAATGTGCACATCCTGTATG
TGTGTGAAGGATCAGGAAGCACAAGAACCCAAACCTAGGAAACGGGCTAATTCTTGCTCC
ATT

ORF Protein

Cloned ORF protein sequence for pFN21AE9414 Download

>KIEE9414  361 aa
MNESRWTEWRILNMSSGIVNVSERHSCPLGFGHYSVVDVCIFETVVIVLLTFLIIAGNLT
VIFVFHCAPLLHHYTTSYFIQTMAYADLFVGVSCLVPTLSLLHYSTGVHESLTCQVFGYI
ISVLKSVSMACLACISVDRYLAITKPLSYNQLVTPCRLRICIILIWIYSCLIFLPSFFGW
GKPGYHGDIFEWCATSWLTSAYFTGFIVCLLYAPAAFVVCFTYFHIFKICRQHTKEINDR
RARFPSHEVDSSRETGHSPDRRYAMVLFRITSVFYMLWLPYIIYFLLESSRVLDNPTLSF
LTTWLAISNSFCNCVIYSLSNSVFRLGLRRLSETMCTSCMCVKDQEAQEPKPRKRANSCS
I

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9414 Download

>pFN21AE9414  5788 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATGAATCCAGGTGGACTGAATGGAGGATCCT
GAACATGAGCAGTGGCATTGTGAATGTGTCCGAGCGTCACTCCTGCCCACTTGGATTTGG
CCACTACAGTGTGGTGGATGTCTGCATCTTCGAGACAGTGGTTATTGTGTTGCTGACATT
TCTGATCATTGCTGGGAATCTAACAGTTATCTTTGTCTTTCATTGTGCTCCACTGTTACA
TCATTATACTACCAGCTATTTCATTCAGACGATGGCATATGCTGATCTTTTCGTTGGAGT
TAGCTGCTTGGTTCCTACTCTGTCACTTCTCCACTACTCCACAGGTGTCCACGAGTCATT
GACTTGCCAGGTTTTTGGATATATCATCTCAGTTCTAAAAAGTGTTTCTATGGCATGTCT
TGCTTGCATCAGTGTGGATCGTTATCTTGCAATAACCAAGCCTCTTTCCTACAATCAACT
GGTCACCCCTTGTCGCTTGAGAATTTGCATTATTTTGATCTGGATCTACTCCTGCCTAAT
TTTCTTGCCTTCCTTTTTTGGCTGGGGGAAACCTGGTTACCATGGTGACATTTTTGAATG
GTGTGCCACGTCTTGGCTCACCAGTGCCTATTTTACTGGCTTTATTGTTTGTTTACTTTA
TGCTCCTGCTGCCTTTGTTGTCTGCTTCACTTACTTCCACATTTTCAAAATTTGCCGTCA
GCACACCAAAGAGATAAATGACCGAAGAGCCCGATTCCCTAGTCATGAGGTAGATTCTTC
CAGAGAGACTGGACACAGCCCTGACCGTCGCTACGCCATGGTTTTGTTTAGGATAACCAG
TGTATTTTATATGCTGTGGCTCCCCTATATAATTTACTTTCTTCTAGAAAGCTCCCGGGT
CTTGGACAATCCAACTCTGTCCTTCTTAACAACCTGGCTTGCAATAAGTAATAGTTTTTG
TAACTGTGTAATATACAGCCTCTCCAACAGCGTTTTCCGGCTAGGCCTCCGAAGACTGTC
TGAGACAATGTGCACATCCTGTATGTGTGTGAAGGATCAGGAAGCACAAGAACCCAAACC
TAGGAAACGGGCTAATTCTTGCTCCATTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT