ASCC2 ORF clone FHC27237

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC27237

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Clone info.

Species

human

Clone Name

pFN21AE2083 Sequence (with vector) Restriction Map

Product ID

FHC27237

ORF Sequence

DNA sequence (2271 bp)
Protein sequence (757 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590901]

Gene

KIEE2083

Symbol

ASCC2

Alias : ASC1p100, p100

Description

activating signal cointegrator 1 complex subunit 2, transcript variant 1

Original Clone ID

cs02983

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2271 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 757 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13869.1 0 100.0 ASCC2 CCDS56226.1 7.2e-201 90.0 ASCC2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003892 469 508 PF02845 Ubiquitin system component Cue HMMSmart IPR003892 467 509 SM00546 Ubiquitin system component Cue ProfileScan IPR003892 467 510 PS51140 Ubiquitin system component Cue
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_016884487 757 614216 100.0 100.0 XP_016884488 757 614216 100.0 100.0 XP_016884489 757 614216 100.0 100.0 XP_005261832 757 614216 100.0 100.0 NP_115580 757 614216 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2083 Download

>KIEE2083  2271 bp
ATGCCAGCTCTGCCCCTGGACCAACTCCAGATCACCCACAAGGACCCGAAGACAGGAAAG
CTGAGGACTTCACCAGCGCTGCACCCCGAGCAGAAGGCAGACCGGTATTTTGTGTTATAC
AAACCGCCCCCTAAAGACAACATTCCCGCCCTAGTGGAGGAGTACCTGGAACGCGCCACC
TTCGTAGCCAATGACCTCGACTGGCTCCTGGCCTTGCCTCACGATAAATTCTGGTGCCAG
GTGATCTTTGACGAGACTCTACAGAAGTGCCTGGACTCCTACCTGCGCTATGTCCCCCGC
AAATTCGACGAGGGGGTGGCCTCAGCCCCTGAGGTTGTTGACATGCAGAAGCGCCTCCAT
CGAAGTGTTTTTCTCACCTTCCTCCGCATGTCCACTCACAAGGAATCCAAAGATCACTTC
ATTTCCCCTTCTGCGTTTGGAGAAATCCTCTACAATAACTTCCTCTTTGACATTCCAAAG
ATCCTGGACCTCTGCGTGCTCTTTGGAAAAGGCAACTCACCACTGCTCCAGAAGATGATA
GGAAACATCTTTACACAGCAGCCAAGTTACTACAGTGACCTGGATGAAACCCTGCCTACC
ATCCTTCAGGTCTTCAGCAATATCCTCCAGCACTGTGGTTTGCAAGGGGACGGGGCCAAT
ACCACACCCCAGAAGCTTGAGGAGAGGGGCCGATTGACCCCCAGTGACATGCCTCTCCTG
GAATTAAAGGACATTGTTCTCTACCTTTGTGATACCTGCACCACACTTTGGGCCTTTCTG
GATATCTTCCCTTTGGCTTGCCAGACCTTCCAGAAGCACGACTTTTGTTACAGACTAGCT
TCCTTCTACGAAGCAGCAATTCCCGAAATGGAGTCTGCAATTAAGAAGAGGAGGCTTGAA
GATAGCAAGCTTCTTGGTGACCTGTGGCAGAGGCTCTCCCATTCCAGGAAGAAGCTAATG
GAGATTTTCCACATCATCCTGAACCAGATCTGCCTCCTTCCCATCCTAGAAAGCAGCTGT
GACAACATTCAGGGCTTCATCGAAGAGTTCCTTCAGATCTTCAGCTCCTTGCTGCAGGAG
AAGAGGTTCCTCCGGGACTATGATGCACTCTTCCCCGTGGCCGAAGACATCAGCTTGCTG
CAGCAGGCCTCATCAGTCTTGGACGAGACGCGGACTGCCTACATCCTCCAGGCAGTCGAG
AGTGCATGGGAAGGGGTGGACAGACGGAAAGCCACAGATGCTAAAGACCCATCGGTGATT
GAGGAGCCTAATGGGGAGCCTAACGGGGTCACGGTGACAGCAGAGGCAGTCAGTCAAGCA
TCATCACATCCGGAGAACTCGGAGGAAGAGGAGTGCATGGGAGCAGCCGCGGCTGTGGGC
CCTGCCATGTGTGGGGTGGAACTGGACTCTCTCATCTCCCAAGTGAAGGACCTGCTGCCA
GACCTTGGTGAGGGCTTCATCCTGGCCTGCCTGGAGTACTACCACTACGACCCAGAGCAG
GTGATCAACAATATCCTGGAGGAGCGGCTGGCCCCCACCCTCAGCCAGCTGGACCGCAAC
CTAGACAGAGAAATGAAACCAGACCCTACACCCCTGCTGACGTCTCGCCACAACGTCTTC
CAGAATGACGAGTTTGATGTGTTCAGCAGGGACTCAGTAGACCTGAGCCGGGTGCACAAG
GGCAAGAGCACCAGGAAGGAGGAAAACACGCGGAGTTTGCTGAACGACAAGCGTGCAGTG
GCGGCACAGCGGCAGCGCTACGAGCAGTACAGCGTGGTGGTGGAGGAGGTGCCACTGCAG
CCAGGCGAGAGCCTGCCCTACCACAGTGTCTACTACGAGGATGAGTACGATGACACATAC
GATGGCAACCAGGTGGGCGCCAATGATGCAGACTCTGATGACGAGCTCATCAGCCGCAGG
CCATTCACCATCCCTCAGGTGCTGAGAACCAAAGTGCCTAGAGAAGGGCAGGAGGAGGAT
GACGACGATGAGGAAGACGATGCTGACGAGGAGGCTCCCAAGCCCGACCATTTTGTTCAG
GACCCTGCAGTGCTGAGAGAGAAGGCAGAAGCCAGGCGCATGGCCTTTCTCGCCAAGAAA
GGGTACCGGCATGACAGCTCAACAGCAGTGGCCGGCAGCCCCCGAGGCCATGGGCAGAGC
CGCGAGACAACCCAGGAACGCAGGAAGAAGGAAGCCAACAAGGCGACAAGAGCCAACCAC
AACCGGAGAACCATGGCCGACCGCAAGAGGAGCAAAGGCATGATCCCATCC

ORF Protein

Cloned ORF protein sequence for pFN21AE2083 Download

>KIEE2083  757 aa
MPALPLDQLQITHKDPKTGKLRTSPALHPEQKADRYFVLYKPPPKDNIPALVEEYLERAT
FVANDLDWLLALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFDEGVASAPEVVDMQKRLH
RSVFLTFLRMSTHKESKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQKMI
GNIFTQQPSYYSDLDETLPTILQVFSNILQHCGLQGDGANTTPQKLEERGRLTPSDMPLL
ELKDIVLYLCDTCTTLWAFLDIFPLACQTFQKHDFCYRLASFYEAAIPEMESAIKKRRLE
DSKLLGDLWQRLSHSRKKLMEIFHIILNQICLLPILESSCDNIQGFIEEFLQIFSSLLQE
KRFLRDYDALFPVAEDISLLQQASSVLDETRTAYILQAVESAWEGVDRRKATDAKDPSVI
EEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLP
DLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVF
QNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQ
PGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEED
DDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQS
RETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2083 Download

>pFN21AE2083  6976 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCAGCTCTGCCCCTGGACCAACTCCAGATCAC
CCACAAGGACCCGAAGACAGGAAAGCTGAGGACTTCACCAGCGCTGCACCCCGAGCAGAA
GGCAGACCGGTATTTTGTGTTATACAAACCGCCCCCTAAAGACAACATTCCCGCCCTAGT
GGAGGAGTACCTGGAACGCGCCACCTTCGTAGCCAATGACCTCGACTGGCTCCTGGCCTT
GCCTCACGATAAATTCTGGTGCCAGGTGATCTTTGACGAGACTCTACAGAAGTGCCTGGA
CTCCTACCTGCGCTATGTCCCCCGCAAATTCGACGAGGGGGTGGCCTCAGCCCCTGAGGT
TGTTGACATGCAGAAGCGCCTCCATCGAAGTGTTTTTCTCACCTTCCTCCGCATGTCCAC
TCACAAGGAATCCAAAGATCACTTCATTTCCCCTTCTGCGTTTGGAGAAATCCTCTACAA
TAACTTCCTCTTTGACATTCCAAAGATCCTGGACCTCTGCGTGCTCTTTGGAAAAGGCAA
CTCACCACTGCTCCAGAAGATGATAGGAAACATCTTTACACAGCAGCCAAGTTACTACAG
TGACCTGGATGAAACCCTGCCTACCATCCTTCAGGTCTTCAGCAATATCCTCCAGCACTG
TGGTTTGCAAGGGGACGGGGCCAATACCACACCCCAGAAGCTTGAGGAGAGGGGCCGATT
GACCCCCAGTGACATGCCTCTCCTGGAATTAAAGGACATTGTTCTCTACCTTTGTGATAC
CTGCACCACACTTTGGGCCTTTCTGGATATCTTCCCTTTGGCTTGCCAGACCTTCCAGAA
GCACGACTTTTGTTACAGACTAGCTTCCTTCTACGAAGCAGCAATTCCCGAAATGGAGTC
TGCAATTAAGAAGAGGAGGCTTGAAGATAGCAAGCTTCTTGGTGACCTGTGGCAGAGGCT
CTCCCATTCCAGGAAGAAGCTAATGGAGATTTTCCACATCATCCTGAACCAGATCTGCCT
CCTTCCCATCCTAGAAAGCAGCTGTGACAACATTCAGGGCTTCATCGAAGAGTTCCTTCA
GATCTTCAGCTCCTTGCTGCAGGAGAAGAGGTTCCTCCGGGACTATGATGCACTCTTCCC
CGTGGCCGAAGACATCAGCTTGCTGCAGCAGGCCTCATCAGTCTTGGACGAGACGCGGAC
TGCCTACATCCTCCAGGCAGTCGAGAGTGCATGGGAAGGGGTGGACAGACGGAAAGCCAC
AGATGCTAAAGACCCATCGGTGATTGAGGAGCCTAATGGGGAGCCTAACGGGGTCACGGT
GACAGCAGAGGCAGTCAGTCAAGCATCATCACATCCGGAGAACTCGGAGGAAGAGGAGTG
CATGGGAGCAGCCGCGGCTGTGGGCCCTGCCATGTGTGGGGTGGAACTGGACTCTCTCAT
CTCCCAAGTGAAGGACCTGCTGCCAGACCTTGGTGAGGGCTTCATCCTGGCCTGCCTGGA
GTACTACCACTACGACCCAGAGCAGGTGATCAACAATATCCTGGAGGAGCGGCTGGCCCC
CACCCTCAGCCAGCTGGACCGCAACCTAGACAGAGAAATGAAACCAGACCCTACACCCCT
GCTGACGTCTCGCCACAACGTCTTCCAGAATGACGAGTTTGATGTGTTCAGCAGGGACTC
AGTAGACCTGAGCCGGGTGCACAAGGGCAAGAGCACCAGGAAGGAGGAAAACACGCGGAG
TTTGCTGAACGACAAGCGTGCAGTGGCGGCACAGCGGCAGCGCTACGAGCAGTACAGCGT
GGTGGTGGAGGAGGTGCCACTGCAGCCAGGCGAGAGCCTGCCCTACCACAGTGTCTACTA
CGAGGATGAGTACGATGACACATACGATGGCAACCAGGTGGGCGCCAATGATGCAGACTC
TGATGACGAGCTCATCAGCCGCAGGCCATTCACCATCCCTCAGGTGCTGAGAACCAAAGT
GCCTAGAGAAGGGCAGGAGGAGGATGACGACGATGAGGAAGACGATGCTGACGAGGAGGC
TCCCAAGCCCGACCATTTTGTTCAGGACCCTGCAGTGCTGAGAGAGAAGGCAGAAGCCAG
GCGCATGGCCTTTCTCGCCAAGAAAGGGTACCGGCATGACAGCTCAACAGCAGTGGCCGG
CAGCCCCCGAGGCCATGGGCAGAGCCGCGAGACAACCCAGGAACGCAGGAAGAAGGAAGC
CAACAAGGCGACAAGAGCCAACCACAACCGGAGAACCATGGCCGACCGCAAGAGGAGCAA
AGGCATGATCCCATCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT