GPRC5D ORF clone FHC24628

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Halotag ORF Clone FHC24628

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Clone info.

Species

human

Clone Name

pFN21AE9440 Sequence (with vector) Restriction Map

Product ID

FHC24628

ORF Sequence

DNA sequence (1035 bp)
Protein sequence (345 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9440

Symbol

GPRC5D

Description

G protein-coupled receptor, class C, group 5, member D

Original Clone ID

cp99440

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1035 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 345 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8658.1 5.7e-143 100.0 GPRC5D CCDS8657.1 1.1e-54 45.7 GPRC5A CCDS10581.1 1.7e-36 35.2 GPRC5B CCDS42378.1 2.6e-17 37.2 GPRC5C CCDS11699.1 2.8e-17 37.2 GPRC5C The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017978 33 265 PF00003 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 27 ESLAILGIVVTILLLLAFLFLM 48 PRIMARY 22 2 60 LPTQLLFLLSVLGLFGLAFAFII 82 PRIMARY 23 3 93 YFLFGVLFALCFSCLLAHASNLV 115 PRIMARY 23 4 131 CIAIGCSLLQIIIATEYVTLIMT 153 PRIMARY 23 5 169 DFVVLLVYVLFLMALTFFVSKAT 191 PRIMARY 23 6 205 LIFITVLFSIIIWVVWISMLLRG 227 PRIMARY 23 7 242 VCIALVTNAWVFLLLYIVPELCI 264 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_061124 345 607437 100.0 100.0 XP_016875071 345 607437 100.0 100.0 XP_005253478 345 607437 100.0 100.0 XP_016875072 300 607437 100.0 86.4 NP_003970 357 604138 45.7 95.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9440 Download

>KIEE9440  1035 bp
ATGTACAAGGACTGCATCGAGTCCACTGGAGACTATTTTCTTCTCTGTGACGCCGAGGGG
CCATGGGGCATCATTCTGGAGTCCCTGGCCATACTTGGCATCGTGGTCACAATTCTGCTA
CTCTTAGCATTTCTCTTCCTCATGCGAAAGATCCAAGACTGCAGCCAGTGGAATGTCCTC
CCCACCCAGCTCCTCTTCCTCCTGAGTGTCCTGGGGCTCTTCGGACTCGCTTTTGCCTTC
ATCATCGAGCTCAATCAACAAACTGCCCCCGTACGCTACTTTCTCTTTGGGGTTCTCTTT
GCTCTCTGTTTCTCATGCCTCTTAGCTCATGCCTCCAATCTAGTGAAGCTGGTTCGGGGT
TGTGTCTCCTTCTCCTGGACGACAATTCTGTGCATTGCTATTGGTTGCAGTCTGTTGCAA
ATCATTATTGCCACTGAGTATGTGACTCTCATCATGACCAGAGGTATGATGTTTGTGAAT
ATGACACCCTGCCAGCTCAATGTGGACTTTGTTGTACTCCTGGTCTATGTCCTCTTCCTG
ATGGCCCTCACATTCTTCGTCTCCAAAGCCACCTTCTGTGGCCCGTGTGAGAACTGGAAG
CAGCATGGAAGGCTCATCTTTATCACTGTGCTCTTCTCCATCATCATCTGGGTGGTGTGG
ATCTCCATGCTCCTGAGAGGCAACCCGCAGTTCCAGCGACAGCCCCAGTGGGACGACCCG
GTCGTCTGCATTGCTCTGGTCACCAACGCATGGGTTTTCCTGCTGCTGTACATCGTCCCT
GAGCTCTGCATTCTCTACAGATCGTGTAGACAGGAGTGCCCTTTACAAGGCAATGCCTGC
CCCGTCACAGCCTACCAACACAGCTTCCAAGTGGAGAACCAGGAGCTCTCCAGAGCCCGA
GACAGTGATGGAGCTGAGGAGGATGTAGCATTAACTTCATATGGTACTCCCATTCAGCCG
CAGACTGTTGATCCCACACAAGAGTGTTTCATCCCACAGGCTAAACTAAGCCCCCAGCAA
GATGCAGGAGGAGTA

ORF Protein

Cloned ORF protein sequence for pFN21AE9440 Download

>KIEE9440  345 aa
MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVL
PTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRG
CVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFL
MALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDP
VVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRAR
DSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9440 Download

>pFN21AE9440  5740 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTACAAGGACTGCATCGAGTCCACTGGAGACTA
TTTTCTTCTCTGTGACGCCGAGGGGCCATGGGGCATCATTCTGGAGTCCCTGGCCATACT
TGGCATCGTGGTCACAATTCTGCTACTCTTAGCATTTCTCTTCCTCATGCGAAAGATCCA
AGACTGCAGCCAGTGGAATGTCCTCCCCACCCAGCTCCTCTTCCTCCTGAGTGTCCTGGG
GCTCTTCGGACTCGCTTTTGCCTTCATCATCGAGCTCAATCAACAAACTGCCCCCGTACG
CTACTTTCTCTTTGGGGTTCTCTTTGCTCTCTGTTTCTCATGCCTCTTAGCTCATGCCTC
CAATCTAGTGAAGCTGGTTCGGGGTTGTGTCTCCTTCTCCTGGACGACAATTCTGTGCAT
TGCTATTGGTTGCAGTCTGTTGCAAATCATTATTGCCACTGAGTATGTGACTCTCATCAT
GACCAGAGGTATGATGTTTGTGAATATGACACCCTGCCAGCTCAATGTGGACTTTGTTGT
ACTCCTGGTCTATGTCCTCTTCCTGATGGCCCTCACATTCTTCGTCTCCAAAGCCACCTT
CTGTGGCCCGTGTGAGAACTGGAAGCAGCATGGAAGGCTCATCTTTATCACTGTGCTCTT
CTCCATCATCATCTGGGTGGTGTGGATCTCCATGCTCCTGAGAGGCAACCCGCAGTTCCA
GCGACAGCCCCAGTGGGACGACCCGGTCGTCTGCATTGCTCTGGTCACCAACGCATGGGT
TTTCCTGCTGCTGTACATCGTCCCTGAGCTCTGCATTCTCTACAGATCGTGTAGACAGGA
GTGCCCTTTACAAGGCAATGCCTGCCCCGTCACAGCCTACCAACACAGCTTCCAAGTGGA
GAACCAGGAGCTCTCCAGAGCCCGAGACAGTGATGGAGCTGAGGAGGATGTAGCATTAAC
TTCATATGGTACTCCCATTCAGCCGCAGACTGTTGATCCCACACAAGAGTGTTTCATCCC
ACAGGCTAAACTAAGCCCCCAGCAAGATGCAGGAGGAGTAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT