GPR33 ORF clone FHC12604

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Halotag ORF Clone FHC12604

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Clone info.

Species

human

Clone Name

pFN21AE3807 Sequence (with vector) Restriction Map

Product ID

FHC12604

ORF Sequence

DNA sequence (999 bp)
Protein sequence (333 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3807

Symbol

GPR33

Description

G protein-coupled receptor 33 (gene/pseudogene), transcript variant 1, coding

Original Clone ID

cp93807

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 999 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 333 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS73628.1 1.1e-109 100.0 GPR33 CCDS41829.1 4.2e-33 36.8 CMKLR1 CCDS44965.1 4.2e-33 36.8 CMKLR1 CCDS12840.1 8.1e-29 33.0 FPR2 CCDS12841.1 2.7e-28 33.0 FPR3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 31 55 PR00237 GPCR IPR000826 55 68 PR00526 Formyl/methionyl peptide receptor IPR000826 75 91 PR00526 Formyl/methionyl peptide receptor IPR000826 102 119 PR00526 Formyl/methionyl peptide receptor IPR000276 108 130 PR00237 GPCR IPR000826 127 143 PR00526 Formyl/methionyl peptide receptor IPR000276 144 165 PR00237 GPCR IPR000826 203 216 PR00526 Formyl/methionyl peptide receptor IPR000276 206 229 PR00237 GPCR IPR000826 228 245 PR00526 Formyl/methionyl peptide receptor IPR000276 243 267 PR00237 GPCR IPR000276 281 307 PR00237 GPCR IPR000826 303 322 PR00526 Formyl/methionyl peptide receptor HMMPfam IPR000276 47 299 PF00001 GPCR ProfileScan IPR017452 46 299 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 29 KMIIALSLYISSIIGTITNGLYL 51 PRIMARY 23 2 64 TLLFFHLILSYFISTMILPFMAT 86 SECONDARY 23 3 110 LGMFTSVFFLSAIGLDRYLLTLH 132 SECONDARY 23 4 145 ASSIVLGVWISAAALSIPYLIFR 167 SECONDARY 23 5 203 VACFISRFLLGFLLPFFIIIFCY 225 PRIMARY 23 6 244 FKVMMTAIISFFVCWMPYHIHQG 266 PRIMARY 23 7 279 LTLILTVLTTSFNTIFSPTLYLF 301 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001184113 333 610118 100.0 100.0 XP_016874309 371 602351 36.8 95.8 NP_004063 371 602351 36.8 95.8 NP_001135816 373 602351 36.8 95.8 NP_001135817 373 602351 36.8 95.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3807 Download

>KIEE3807  999 bp
ATGGATCTGATCAACTCTACTGATTACCTGATCAATGCCTCTACTTTAGTAAGAAACAGC
ACTCAGTTTCTAGCTCCTGCATCAAAAATGATTATTGCCCTTTCTTTGTACATTTCATCT
ATAATTGGTACCATCACCAATGGCCTCTATCTATGGGTGCTAAGATTCAAGATGAAACAG
ACTGTCAATACTCTCTTATTTTTTCATCTCATTCTCTCTTATTTTATTTCAACAATGATT
CTGCCATTTATGGCCACCTCCCAACTTCAAGACAATCACTGGAACTTTGGAACTGCCTTG
TGCAAGGTCTTCAATGGCACTTTGTCTCTGGGGATGTTCACCTCTGTTTTCTTCCTTTCG
GCCATCGGTCTTGATCGTTACCTTCTCACTCTTCACCCAGTGTGGTCCCAGCAGCACCGA
ACCCCGCGCTGGGCTTCCAGCATTGTCCTGGGAGTCTGGATTTCAGCCGCTGCCCTCAGC
ATCCCCTATTTGATTTTCAGAGAGACACATCATGACCGTAAAGGAAAGGTGACTTGCCAA
AATAACTATGCTGTGTCTACTAACTGGGAAAGCAAGGAGATGCAAGCATCAAGGCAGTGG
ATTCATGTGGCCTGTTTCATCAGCCGCTTCTTGCTGGGCTTTCTTCTGCCTTTCTTCATC
ATCATCTTTTGTTATGAAAGAGTAGCCAGCAAGGTGAAAGAGAGGAGCCTGTTTAAATCC
AGCAAGCCCTTCAAAGTTATGATGACTGCCATTATCTCTTTCTTTGTGTGTTGGATGCCC
TACCATATACACCAGGGCTTACTTCTCACTACGAACCAGTCACTACTTTTAGAGTTGACT
TTGATACTTACAGTGCTAACCACTTCTTTCAATACTATCTTTTCTCCCACACTCTACTTA
TTTGTTGGGGAGAATTTCAAAAAGGTCTTCAAGAAGTCCATTCTTGCTCTGTTTGAGTCA
ACATTTAGTGAAGATTCTTCTGTAGAAAGGACACAAACC

ORF Protein

Cloned ORF protein sequence for pFN21AE3807 Download

>KIEE3807  333 aa
MDLINSTDYLINASTLVRNSTQFLAPASKMIIALSLYISSIIGTITNGLYLWVLRFKMKQ
TVNTLLFFHLILSYFISTMILPFMATSQLQDNHWNFGTALCKVFNGTLSLGMFTSVFFLS
AIGLDRYLLTLHPVWSQQHRTPRWASSIVLGVWISAAALSIPYLIFRETHHDRKGKVTCQ
NNYAVSTNWESKEMQASRQWIHVACFISRFLLGFLLPFFIIIFCYERVASKVKERSLFKS
SKPFKVMMTAIISFFVCWMPYHIHQGLLLTTNQSLLLELTLILTVLTTSFNTIFSPTLYL
FVGENFKKVFKKSILALFESTFSEDSSVERTQT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3807 Download

>pFN21AE3807  5704 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATCTGATCAACTCTACTGATTACCTGATCAA
TGCCTCTACTTTAGTAAGAAACAGCACTCAGTTTCTAGCTCCTGCATCAAAAATGATTAT
TGCCCTTTCTTTGTACATTTCATCTATAATTGGTACCATCACCAATGGCCTCTATCTATG
GGTGCTAAGATTCAAGATGAAACAGACTGTCAATACTCTCTTATTTTTTCATCTCATTCT
CTCTTATTTTATTTCAACAATGATTCTGCCATTTATGGCCACCTCCCAACTTCAAGACAA
TCACTGGAACTTTGGAACTGCCTTGTGCAAGGTCTTCAATGGCACTTTGTCTCTGGGGAT
GTTCACCTCTGTTTTCTTCCTTTCGGCCATCGGTCTTGATCGTTACCTTCTCACTCTTCA
CCCAGTGTGGTCCCAGCAGCACCGAACCCCGCGCTGGGCTTCCAGCATTGTCCTGGGAGT
CTGGATTTCAGCCGCTGCCCTCAGCATCCCCTATTTGATTTTCAGAGAGACACATCATGA
CCGTAAAGGAAAGGTGACTTGCCAAAATAACTATGCTGTGTCTACTAACTGGGAAAGCAA
GGAGATGCAAGCATCAAGGCAGTGGATTCATGTGGCCTGTTTCATCAGCCGCTTCTTGCT
GGGCTTTCTTCTGCCTTTCTTCATCATCATCTTTTGTTATGAAAGAGTAGCCAGCAAGGT
GAAAGAGAGGAGCCTGTTTAAATCCAGCAAGCCCTTCAAAGTTATGATGACTGCCATTAT
CTCTTTCTTTGTGTGTTGGATGCCCTACCATATACACCAGGGCTTACTTCTCACTACGAA
CCAGTCACTACTTTTAGAGTTGACTTTGATACTTACAGTGCTAACCACTTCTTTCAATAC
TATCTTTTCTCCCACACTCTACTTATTTGTTGGGGAGAATTTCAAAAAGGTCTTCAAGAA
GTCCATTCTTGCTCTGTTTGAGTCAACATTTAGTGAAGATTCTTCTGTAGAAAGGACACA
AACCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT