KCNN2 ORF clone FHC12091

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC12091

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Clone info.

Species

human

Clone Name

pFN21AB9280 Sequence (with vector) Restriction Map

Product ID

FHC12091

Flexi Clone PID

FXC12091

ORF Sequence

DNA sequence (1737 bp)
Protein sequence (579 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB9280

Symbol

KCNN2

Alias : KCa2.2, SK2, SKCA2, SKCa 2, hSK2

Description

potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 2, transcript variant 1

Original Clone ID

cp02280

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1737 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 579 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4114.1 6.4e-135 100.0 KCNN2 CCDS30880.1 1.8e-90 73.7 KCNN3 CCDS67611.1 2.1e-82 70.3 KCNN1 CCDS1072.1 4.4e-77 81.1 KCNN3 CCDS43352.1 4.7e-50 100.0 KCNN2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003931 120 140 PR01451 Potassium channel IPR003931 220 238 PR01451 Potassium channel HMMPfam IPR011996 120 237 PF03530 Potassium channel IPR013099 319 397 PF07885 Ion transport 2 IPR004178 412 487 PF02888 Calmodulin-binding domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 135 SDYALIFGMFGIVVMVIETELSW 157 PRIMARY 23 2 168 LALKCLISLSTIILLGLIIVYHA 190 PRIMARY 23 3 208 AMTYERIFFICLEILVCAIHPIP 230 PRIMARY 23 4 254 VDIILSIPMFLRLYLIARVMLLH 276 SECONDARY 23 5 306 MTICPGTVLLVFSISLWIIAAWT 328 PRIMARY 23 6 342 TSNFLGAMWLISITFLSIGYGDM 364 SECONDARY 23 7 373 GVCLLTGIMGAGCTALVVAVVA 394 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_067627 579 605879 100.0 100.0 XP_011541689 857 605879 100.0 100.0 XP_011541691 857 605879 100.0 100.0 NP_002240 731 602983 73.7 100.0 XP_005259964 623 602982 70.7 92.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9280 Download

>KIBB9280  1737 bp
ATGAGCAGCTGCAGGTACAACGGGGGCGTCATGCGGCCGCTCAGCAACTTGAGCGCGTCC
CGCCGGAACCTCCACGAGATGGACTCAGAGGCGCAGCCCCTGCAGCCCCCCGCGTCTGTC
GGAGGAGGTGGCGGCGCGTCCTCCCCGTCTGCAGCCGCTGCCGCCGCCGCCGCTGTTTCG
TCCTCAGCCCCCGAGATCGTGGTGTCTAAGCCCGAGCACAACAACTCCAACAACCTGGCG
CTCTATGGAACCGGCGGCGGAGGCAGCACTGGAGGAGGCGGCGGCGGTGGCGGGAGCGGG
CACGGCAGCAGCAGTGGCACCAAGTCCAGCAAAAAGAAAAACCAGAACATCGGCTACAAG
CTGGGCCACCGGCGCGCCCTGTTCGAAAAGCGCAAGCGGCTCAGCGACTACGCGCTCATC
TTCGGCATGTTCGGCATCGTGGTCATGGTCATCGAGACCGAGCTGTCGTGGGGCGCCTAC
GACAAGGCGTCGCTGTATTCCTTAGCTCTGAAATGCCTTATCAGTCTCTCCACGATCATC
CTGCTCGGTCTGATCATCGTGTACCACGCCAGGGAAATACAGTTGTTCATGGTGGACAAT
GGAGCAGATGACTGGAGAATAGCCATGACTTATGAGCGTATTTTCTTCATCTGCTTGGAA
ATACTGGTGTGTGCTATTCATCCCATACCTGGGAATTATACATTCACATGGACGGCCCGG
CTTGCCTTCTCCTATGCCCCATCCACAACCACCGCTGATGTGGATATTATTTTATCTATA
CCAATGTTCTTAAGACTCTATCTGATTGCCAGAGTCATGCTTTTACATAGCAAACTTTTC
ACTGATGCCTCCTCTAGAAGCATTGGAGCACTTAATAAGATAAACTTCAATACACGTTTT
GTTATGAAGACTTTAATGACTATATGCCCAGGAACTGTACTCTTGGTTTTTAGTATCTCA
TTATGGATAATTGCCGCATGGACTGTCCGAGCTTGTGAAAGGTACCATGATCAACAGGAT
GTTACTAGCAACTTCCTTGGAGCGATGTGGTTGATATCAATAACTTTTCTCTCCATTGGT
TATGGTGACATGGTACCTAACACATACTGTGGAAAAGGAGTCTGCTTACTTACTGGAATT
ATGGGTGCTGGTTGCACAGCCCTGGTGGTAGCTGTAGTGGCAAGGAAGCTAGAACTTACC
AAAGCAGAAAAACACGTGCACAATTTCATGATGGATACTCAGCTGACTAAAAGAGTAAAA
AATGCAGCTGCCAATGTACTCAGGGAAACATGGCTAATTTACAAAAATACAAAGCTAGTG
AAAAAGATAGATCATGCAAAAGTAAGAAAACATCAACGAAAATTCCTGCAAGCTATTCAT
CAATTAAGAAGTGTAAAAATGGAGCAGAGGAAACTGAATGACCAAGCAAACACTTTGGTG
GACTTGGCAAAGACCCAGAACATCATGTATGATATGATTTCTGACTTAAACGAAAGGAGT
GAAGACTTCGAGAAGAGGATTGTTACCCTGGAAACAAAACTAGAGACTTTGATTGGTAGC
ATCCACGCCCTCCCTGGGCTCATAAGCCAGACCATCAGGCAGCAGCAGAGAGATTTCATT
GAGGCTCAGATGGAGAGCTACGACAAGCACGTCACTTACAATGCTGAGCGGTCCCGGTCC
TCGTCCAGGAGGCGGCGGTCCTCTTCCACAGCACCACCAACTTCATCAGAGAGTAGC

ORF Protein

Cloned ORF protein sequence for pFN21AB9280 Download

>KIBB9280  579 aa
MSSCRYNGGVMRPLSNLSASRRNLHEMDSEAQPLQPPASVGGGGGASSPSAAAAAAAAVS
SSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGSGHGSSSGTKSSKKKNQNIGYK
LGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTII
LLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTAR
LAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRF
VMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIG
YGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVK
NAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLV
DLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFI
EAQMESYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9280 Download

>pFN21AB9280  6442 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGCAGCTGCAGGTACAACGGGGGCGTCATGCG
GCCGCTCAGCAACTTGAGCGCGTCCCGCCGGAACCTCCACGAGATGGACTCAGAGGCGCA
GCCCCTGCAGCCCCCCGCGTCTGTCGGAGGAGGTGGCGGCGCGTCCTCCCCGTCTGCAGC
CGCTGCCGCCGCCGCCGCTGTTTCGTCCTCAGCCCCCGAGATCGTGGTGTCTAAGCCCGA
GCACAACAACTCCAACAACCTGGCGCTCTATGGAACCGGCGGCGGAGGCAGCACTGGAGG
AGGCGGCGGCGGTGGCGGGAGCGGGCACGGCAGCAGCAGTGGCACCAAGTCCAGCAAAAA
GAAAAACCAGAACATCGGCTACAAGCTGGGCCACCGGCGCGCCCTGTTCGAAAAGCGCAA
GCGGCTCAGCGACTACGCGCTCATCTTCGGCATGTTCGGCATCGTGGTCATGGTCATCGA
GACCGAGCTGTCGTGGGGCGCCTACGACAAGGCGTCGCTGTATTCCTTAGCTCTGAAATG
CCTTATCAGTCTCTCCACGATCATCCTGCTCGGTCTGATCATCGTGTACCACGCCAGGGA
AATACAGTTGTTCATGGTGGACAATGGAGCAGATGACTGGAGAATAGCCATGACTTATGA
GCGTATTTTCTTCATCTGCTTGGAAATACTGGTGTGTGCTATTCATCCCATACCTGGGAA
TTATACATTCACATGGACGGCCCGGCTTGCCTTCTCCTATGCCCCATCCACAACCACCGC
TGATGTGGATATTATTTTATCTATACCAATGTTCTTAAGACTCTATCTGATTGCCAGAGT
CATGCTTTTACATAGCAAACTTTTCACTGATGCCTCCTCTAGAAGCATTGGAGCACTTAA
TAAGATAAACTTCAATACACGTTTTGTTATGAAGACTTTAATGACTATATGCCCAGGAAC
TGTACTCTTGGTTTTTAGTATCTCATTATGGATAATTGCCGCATGGACTGTCCGAGCTTG
TGAAAGGTACCATGATCAACAGGATGTTACTAGCAACTTCCTTGGAGCGATGTGGTTGAT
ATCAATAACTTTTCTCTCCATTGGTTATGGTGACATGGTACCTAACACATACTGTGGAAA
AGGAGTCTGCTTACTTACTGGAATTATGGGTGCTGGTTGCACAGCCCTGGTGGTAGCTGT
AGTGGCAAGGAAGCTAGAACTTACCAAAGCAGAAAAACACGTGCACAATTTCATGATGGA
TACTCAGCTGACTAAAAGAGTAAAAAATGCAGCTGCCAATGTACTCAGGGAAACATGGCT
AATTTACAAAAATACAAAGCTAGTGAAAAAGATAGATCATGCAAAAGTAAGAAAACATCA
ACGAAAATTCCTGCAAGCTATTCATCAATTAAGAAGTGTAAAAATGGAGCAGAGGAAACT
GAATGACCAAGCAAACACTTTGGTGGACTTGGCAAAGACCCAGAACATCATGTATGATAT
GATTTCTGACTTAAACGAAAGGAGTGAAGACTTCGAGAAGAGGATTGTTACCCTGGAAAC
AAAACTAGAGACTTTGATTGGTAGCATCCACGCCCTCCCTGGGCTCATAAGCCAGACCAT
CAGGCAGCAGCAGAGAGATTTCATTGAGGCTCAGATGGAGAGCTACGACAAGCACGTCAC
TTACAATGCTGAGCGGTCCCGGTCCTCGTCCAGGAGGCGGCGGTCCTCTTCCACAGCACC
ACCAACTTCATCAGAGAGTAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCT
AGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTG
AGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGA
GCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAA
AAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATG
TGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGG
ATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGG
GGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGC
ACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAG
AGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTT
CCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGT
GCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGC
CCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTA
TCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGAC
TTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAA
TTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACT
ATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGC
CTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACG
ATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTA
GCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTG
CGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGG
TCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATC
TACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGT
GCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTAT
CAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGA
ACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGT
TTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGT
GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGC
GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAA
GCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCT
CCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTA
ACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG
GTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGC
CTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTA
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTG
GTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTT
TGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGG
TCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAA
CGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGG
ATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAA
TTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGC
TGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATC
CACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGT
CCGGCGTAGAGGATCGAGATCT