ZNF282 ORF clone FHC11818

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11818

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Clone info.

Species

human

Clone Name

pFN21AB9774 Sequence (with vector) Restriction Map

Product ID

FHC11818

Flexi Clone PID

FXC11818

Original Clone PID

ORK11818

ORF Sequence

DNA sequence (2013 bp)
Protein sequence (671 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587508]

Gene

KIBB9774

Symbol

ZNF282

Alias : HUB1

Description

zinc finger protein 282, transcript variant 1

Original Clone ID

fk05554

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2013 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 671 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5895.1 9.3e-111 99.9 ZNF282 CCDS78284.1 5.3e-72 99.3 ZNF282 CCDS5894.1 3.2e-31 43.2 ZNF398 CCDS43675.1 1.3e-26 38.8 ZNF777 CCDS56519.1 1.3e-19 38.3 ZNF783 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR022137 93 170 PF12417 Protein of unknown function DUF3669 IPR001909 198 238 PF01352 Krueppel-associated box IPR007087 574 596 PF00096 Zinc finger IPR007087 602 624 PF00096 Zinc finger IPR007087 630 652 PF00096 Zinc finger HMMSmart IPR001909 198 260 SM00349 Krueppel-associated box IPR015880 518 540 SM00355 Zinc finger IPR015880 546 568 SM00355 Zinc finger IPR015880 574 596 SM00355 Zinc finger IPR015880 602 624 SM00355 Zinc finger IPR015880 630 652 SM00355 Zinc finger ProfileScan IPR001909 198 271 PS50805 Krueppel-associated box IPR007087 518 545 PS50157 Zinc finger IPR007087 546 573 PS50157 Zinc finger IPR007087 574 601 PS50157 Zinc finger IPR007087 602 629 PS50157 Zinc finger IPR007087 630 657 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003566 671 603397 99.9 100.0 XP_006716214 672 603397 99.7 100.0 XP_005250013 659 613914 40.0 84.6 XP_005250012 660 613914 39.7 84.6 XP_005250014 647 613914 39.4 83.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9774 Download

>KIBB9774  2013 bp
ATGCAGTTTGTGTCAACACGGCCGCAGCCTCAGCAGCTGGGCATCCAGGGCCTGGGGCTG
GACAGCGGGAGCTGGAGCTGGGCCCAGGCTCTGCCCCCGGAGGAGGTCTGCCACCAGGAG
CCGGCGCTGCGCGGGGAAATGGCCGAGGGAATGCCGCCCATGCAGGCTCAAGAATGGGAC
ATGGACGCCCGGCGGCCAATGCCTTTTCAGTTCCCACCCTTTCCAGATAGGGCACCTGTC
TTCCCCGACCGCATGATGCGAGAGCCCCAGTTGCCCACAGCAGAGATCTCACTCTGGACT
GTGGTGGCTGCCATTCAGGCTGTGGAGAGGAAGGTGGATGCCCAGGCCAGCCAGCTGCTG
AACCTGGAGGGGCGCACGGGGACAGCCGAGAAGAAGCTGGCCGACTGTGAAAAGACGGCC
GTGGAATTTGGGAACCACATGGAGAGCAAGTGGGCCGTGCTGGGGACCCTGCTGCAGGAG
TACGGGCTGCTGCAGAGGCGGCTGGAGAACTTGGAGAACTTGCTGCGCAACAGGAACTTC
TGGGTCCTGCGGCTGCCCCCGGGCAGCAAGGGGGAGGCCCCCAAGGTTCCAGTGACTTTT
GTCGACATTGCTGTGTACTTCTCCGAAGACGAGTGGAAGAACTTGGACGAATGGCAGAAG
GAGCTTTATAACAACCTTGTTAAGGAGAACTACAAAACCCTCATGTCCCTGGACGCGGAG
GGCTCAGTCCCCAAGCCAGATGCTCCAGTCCAGGCTGAGCCCAGGGAAGAACCTTGTGTG
TGGGAGCAGCGCCACCCCGAAGAGAGAGAAATCCCAATGGATCCCGAAGCAGGAGCAGAG
CCCCTGGTGCCTGCGCAGGATGCGTCCTCCCAGGTGAAGCGTGAGGACACCCTGTGTGTC
CGGGGTCAGCGGGGCCTGGAGGAAAGAGCCATCCCTACGGAATCCATTACCGACTCCCCA
ATTTCTGCCCAGGACCTCTTGTCCCGGATTAAACAGGAGGAGCATCAGTGCGTGTGGGAT
CAGCAGGATTTGGCAGACAGAGATATTCCCACGGATCCCAATTCAGAGTCTCTCATCTCA
GCACATGACATTTTGTCATGGATCAAGCAGGAGGAGCAGCCATACCCATGGGGACCACGC
GACTCAATGGACGGAGAGCTTGGATTAGACTCTGGCCCTAGTGACAGCCTGCTGATGGTG
AAGAACCCACCCCCGGCCCCGCCACAGCCCCAGCCCCAGCCCCAGCCACCGCAGCCGCAG
CTGCAGTCGCAGCCCCAGCCCCAGAGCCTGCCCCCCATCGCGGTGGCCGAGAACCCGGGC
GGCCCCCCGAGCCGAGGGCTGCTGGACGACGGTTTCCAGGTGCTGCCCGGGGAGCGTGGC
TCCGGCGAGGCGCCGCCGGGTGGGGACCGCAGCACCGGGGGCGGCGGGGGCGATGGGGGC
GGTGGGGGCGGCGGCGCGGAGGCGGGGACGGGGGCAGGCGGCGGCTGTGGCAGCTGCTGC
CCTGGCGGGCTGCGGCGGAGCCTCCTCCTGCACGGCGCCCGCAGCAAGCCCTACTCGTGC
CCCGAGTGCGGCAAGAGCTTCGGCGTGCGCAAGAGCCTCATCATCCACCACCGCAGCCAC
ACCAAGGAGCGGCCCTACGAGTGCGCTGAGTGCGAGAAGAGCTTCAACTGCCACTCGGGC
CTCATCCGCCACCAGATGACGCACCGCGGCGAGCGGCCCTACAAGTGCTCGGAGTGCGAG
AAGACCTACAGCCGTAAGGAGCACCTGCAGAACCACCAGCGGCTGCACACGGGCGAGCGG
CCTTTCCAATGTGCACTGTGCGGCAAGAGCTTCATCCGCAAGCAGAACCTGCTCAAGCAC
CAGCGCATCCACACGGGCGAGCGCCCCTACACGTGCGGCGAGTGCGGCAAGAGCTTCCGC
TACAAGGAGTCGCTCAAGGACCACCTGCGCGTGCACAGCGGCGGCCCGGGCCCCGGCGCC
CCACGGCAGCTCCCGCCGCCTCCTGAGCAAGAC

ORF Protein

Cloned ORF protein sequence for pFN21AB9774 Download

>KIBB9774  671 aa
MQFVSTRPQPQQLGIQGLGLDSGSWSWAQALPPEEVCHQEPALRGEMAEGMPPMQAQEWD
MDARRPMPFQFPPFPDRAPVFPDRMMREPQLPTAEISLWTVVAAIQAVERKVDAQASQLL
NLEGRTGTAEKKLADCEKTAVEFGNHMESKWAVLGTLLQEYGLLQRRLENLENLLRNRNF
WVLRLPPGSKGEAPKVPVTFVDIAVYFSEDEWKNLDEWQKELYNNLVKENYKTLMSLDAE
GSVPKPDAPVQAEPREEPCVWEQRHPEEREIPMDPEAGAEPLVPAQDASSQVKREDTLCV
RGQRGLEERAIPTESITDSPISAQDLLSRIKQEEHQCVWDQQDLADRDIPTDPNSESLIS
AHDILSWIKQEEQPYPWGPRDSMDGELGLDSGPSDSLLMVKNPPPAPPQPQPQPQPPQPQ
LQSQPQPQSLPPIAVAENPGGPPSRGLLDDGFQVLPGERGSGEAPPGGDRSTGGGGGDGG
GGGGGAEAGTGAGGGCGSCCPGGLRRSLLLHGARSKPYSCPECGKSFGVRKSLIIHHRSH
TKERPYECAECEKSFNCHSGLIRHQMTHRGERPYKCSECEKTYSRKEHLQNHQRLHTGER
PFQCALCGKSFIRKQNLLKHQRIHTGERPYTCGECGKSFRYKESLKDHLRVHSGGPGPGA
PRQLPPPPEQD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9774 Download

>pFN21AB9774  6718 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCAGTTTGTGTCAACACGGCCGCAGCCTCAGCA
GCTGGGCATCCAGGGCCTGGGGCTGGACAGCGGGAGCTGGAGCTGGGCCCAGGCTCTGCC
CCCGGAGGAGGTCTGCCACCAGGAGCCGGCGCTGCGCGGGGAAATGGCCGAGGGAATGCC
GCCCATGCAGGCTCAAGAATGGGACATGGACGCCCGGCGGCCAATGCCTTTTCAGTTCCC
ACCCTTTCCAGATAGGGCACCTGTCTTCCCCGACCGCATGATGCGAGAGCCCCAGTTGCC
CACAGCAGAGATCTCACTCTGGACTGTGGTGGCTGCCATTCAGGCTGTGGAGAGGAAGGT
GGATGCCCAGGCCAGCCAGCTGCTGAACCTGGAGGGGCGCACGGGGACAGCCGAGAAGAA
GCTGGCCGACTGTGAAAAGACGGCCGTGGAATTTGGGAACCACATGGAGAGCAAGTGGGC
CGTGCTGGGGACCCTGCTGCAGGAGTACGGGCTGCTGCAGAGGCGGCTGGAGAACTTGGA
GAACTTGCTGCGCAACAGGAACTTCTGGGTCCTGCGGCTGCCCCCGGGCAGCAAGGGGGA
GGCCCCCAAGGTTCCAGTGACTTTTGTCGACATTGCTGTGTACTTCTCCGAAGACGAGTG
GAAGAACTTGGACGAATGGCAGAAGGAGCTTTATAACAACCTTGTTAAGGAGAACTACAA
AACCCTCATGTCCCTGGACGCGGAGGGCTCAGTCCCCAAGCCAGATGCTCCAGTCCAGGC
TGAGCCCAGGGAAGAACCTTGTGTGTGGGAGCAGCGCCACCCCGAAGAGAGAGAAATCCC
AATGGATCCCGAAGCAGGAGCAGAGCCCCTGGTGCCTGCGCAGGATGCGTCCTCCCAGGT
GAAGCGTGAGGACACCCTGTGTGTCCGGGGTCAGCGGGGCCTGGAGGAAAGAGCCATCCC
TACGGAATCCATTACCGACTCCCCAATTTCTGCCCAGGACCTCTTGTCCCGGATTAAACA
GGAGGAGCATCAGTGCGTGTGGGATCAGCAGGATTTGGCAGACAGAGATATTCCCACGGA
TCCCAATTCAGAGTCTCTCATCTCAGCACATGACATTTTGTCATGGATCAAGCAGGAGGA
GCAGCCATACCCATGGGGACCACGCGACTCAATGGACGGAGAGCTTGGATTAGACTCTGG
CCCTAGTGACAGCCTGCTGATGGTGAAGAACCCACCCCCGGCCCCGCCACAGCCCCAGCC
CCAGCCCCAGCCACCGCAGCCGCAGCTGCAGTCGCAGCCCCAGCCCCAGAGCCTGCCCCC
CATCGCGGTGGCCGAGAACCCGGGCGGCCCCCCGAGCCGAGGGCTGCTGGACGACGGTTT
CCAGGTGCTGCCCGGGGAGCGTGGCTCCGGCGAGGCGCCGCCGGGTGGGGACCGCAGCAC
CGGGGGCGGCGGGGGCGATGGGGGCGGTGGGGGCGGCGGCGCGGAGGCGGGGACGGGGGC
AGGCGGCGGCTGTGGCAGCTGCTGCCCTGGCGGGCTGCGGCGGAGCCTCCTCCTGCACGG
CGCCCGCAGCAAGCCCTACTCGTGCCCCGAGTGCGGCAAGAGCTTCGGCGTGCGCAAGAG
CCTCATCATCCACCACCGCAGCCACACCAAGGAGCGGCCCTACGAGTGCGCTGAGTGCGA
GAAGAGCTTCAACTGCCACTCGGGCCTCATCCGCCACCAGATGACGCACCGCGGCGAGCG
GCCCTACAAGTGCTCGGAGTGCGAGAAGACCTACAGCCGTAAGGAGCACCTGCAGAACCA
CCAGCGGCTGCACACGGGCGAGCGGCCTTTCCAATGTGCACTGTGCGGCAAGAGCTTCAT
CCGCAAGCAGAACCTGCTCAAGCACCAGCGCATCCACACGGGCGAGCGCCCCTACACGTG
CGGCGAGTGCGGCAAGAGCTTCCGCTACAAGGAGTCGCTCAAGGACCACCTGCGCGTGCA
CAGCGGCGGCCCGGGCCCCGGCGCCCCACGGCAGCTCCCGCCGCCTCCTGAGCAAGACGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT