RHOU ORF clone FHC11708

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11708

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Clone info.

Species

human

Clone Name

pFN21AB6307 Sequence (with vector) Restriction Map

Product ID

FHC11708

Flexi Clone PID

FXC11708

ORF Sequence

DNA sequence (774 bp)
Protein sequence (258 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB6307

Symbol

RHOU

Alias : ARHU, CDC42L1, G28K, WRCH1, hG28K

Description

ras homolog family member U, transcript variant 1

Original Clone ID

cs00907

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 774 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 258 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1575.1 1.5e-74 100.0 RHOU CCDS10068.1 3.3e-34 54.4 RHOV CCDS221.1 6.3e-29 60.1 CDC42 CCDS222.1 6.3e-29 60.2 CDC42 CCDS5348.1 1.6e-26 56.1 RAC1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 50 71 PR00449 Ras GTPase IPR001806 73 89 PR00449 Ras GTPase IPR001806 90 112 PR00449 Ras GTPase IPR001806 152 165 PR00449 Ras GTPase IPR001806 200 222 PR00449 Ras GTPase HMMPfam IPR013753 51 219 PF00071 Ras HMMSmart IPR003577 47 226 SM00173 Ras small GTPase IPR003579 50 225 SM00175 Ras small GTPase IPR003578 52 225 SM00174 Small GTPase IPR002041 55 254 SM00176 Ran GTPase HMMTigr IPR005225 49 203 TIGR00231 Small GTP-binding protein domain ProfileScan IPR003578 43 223 PS51420 Small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_067028 258 606366 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6307 Download

>KIBB6307  774 bp
ATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCCGACCGCTGCGAGGCGCCTCCGGTGCCG
CCGCGTCGGGAGCGCGGTGGACGCGGGGGACGCGGGCCTGGGGAGCCGGGGGGCCGGGGG
CGTGCGGGGGGTGCCGAGGGGCGCGGCGTCAAGTGCGTGCTGGTCGGCGACGGCGCGGTG
GGCAAGACGAGCCTGGTGGTGAGCTACACCACCAACGGCTACCCCACCGAGTACATCCCT
ACTGCCTTCGACAACTTCTCCGCGGTGGTGTCTGTGGATGGGCGGCCCGTGAGACTCCAA
CTCTGTGACACTGCCGGACAGGATGAATTTGACAAGCTGAGGCCTCTCTGCTACACCAAC
ACAGACATCTTCCTGCTCTGCTTCAGTGTCGTGAGCCCCTCATCCTTCCAGAACGTCAGT
GAGAAATGGGTGCCGGAGATTCGATGCCACTGTCCCAAAGCCCCCATCATCCTAGTTGGA
ACGCAGTCGGATCTCAGAGAAGATGTCAAAGTCCTCATTGAGTTGGACAAATGCAAAGAA
AAGCCAGTGCCTGAAGAGGCGGCTAAGCTGTGCGCCGAGGAAATCAAAGCCGCCTCCTAC
ATCGAGTGTTCAGCCTTGACTCAAAAAAACCTCAAAGAGGTCTTTGATGCAGCCATCGTC
GCTGGCATTCAATACTCGGACACTCAGCAACAGCCAAAGAAGTCTAAAAGCAGGACTCCA
GATAAAATGAAAAACCTCTCCAAGTCCTGGTGGAAGAAGTACTGCTGTTTCGTA

ORF Protein

Cloned ORF protein sequence for pFN21AB6307 Download

>KIBB6307  258 aa
MPPQQGDPAFPDRCEAPPVPPRRERGGRGGRGPGEPGGRGRAGGAEGRGVKCVLVGDGAV
GKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTN
TDIFLLCFSVVSPSSFQNVSEKWVPEIRCHCPKAPIILVGTQSDLREDVKVLIELDKCKE
KPVPEEAAKLCAEEIKAASYIECSALTQKNLKEVFDAAIVAGIQYSDTQQQPKKSKSRTP
DKMKNLSKSWWKKYCCFV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6307 Download

>pFN21AB6307  5479 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCCCGCAGCAGGGGGACCCCGCGTTCCCCGA
CCGCTGCGAGGCGCCTCCGGTGCCGCCGCGTCGGGAGCGCGGTGGACGCGGGGGACGCGG
GCCTGGGGAGCCGGGGGGCCGGGGGCGTGCGGGGGGTGCCGAGGGGCGCGGCGTCAAGTG
CGTGCTGGTCGGCGACGGCGCGGTGGGCAAGACGAGCCTGGTGGTGAGCTACACCACCAA
CGGCTACCCCACCGAGTACATCCCTACTGCCTTCGACAACTTCTCCGCGGTGGTGTCTGT
GGATGGGCGGCCCGTGAGACTCCAACTCTGTGACACTGCCGGACAGGATGAATTTGACAA
GCTGAGGCCTCTCTGCTACACCAACACAGACATCTTCCTGCTCTGCTTCAGTGTCGTGAG
CCCCTCATCCTTCCAGAACGTCAGTGAGAAATGGGTGCCGGAGATTCGATGCCACTGTCC
CAAAGCCCCCATCATCCTAGTTGGAACGCAGTCGGATCTCAGAGAAGATGTCAAAGTCCT
CATTGAGTTGGACAAATGCAAAGAAAAGCCAGTGCCTGAAGAGGCGGCTAAGCTGTGCGC
CGAGGAAATCAAAGCCGCCTCCTACATCGAGTGTTCAGCCTTGACTCAAAAAAACCTCAA
AGAGGTCTTTGATGCAGCCATCGTCGCTGGCATTCAATACTCGGACACTCAGCAACAGCC
AAAGAAGTCTAAAAGCAGGACTCCAGATAAAATGAAAAACCTCTCCAAGTCCTGGTGGAA
GAAGTACTGCTGTTTCGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT