GPRIN3 ORF clone FHC11609

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11609

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Clone info.

Species

human

Clone Name

pFN21AA2027 Sequence (with vector) Restriction Map

Product ID

FHC11609

Flexi Clone PID

FXC11609

ORF Sequence

DNA sequence (2328 bp)
Protein sequence (776 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527401]

Gene

KIAA2027

Symbol

GPRIN3

Alias : GRIN3

Description

GPRIN family member 3

Original Clone ID

cp00106

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2328 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 776 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34030.1 1.7e-166 100.0 GPRIN3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_938022 776 611241 100.0 100.0 XP_016863533 776 611241 100.0 100.0 XP_005262993 776 611241 100.0 100.0 XP_005262994 776 611241 100.0 100.0 XP_016863532 776 611241 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA2027 Download

>KIAA2027  2328 bp
ATGGGGACTGTACCTGACCCTCTGAGATCAGCTAAAACTTCCCTGATTGCAGCTTCCGGA
AAAGAAGACGATCTAGGAGAGCCACAGGCTGCCTCACCTCGGCATCGACCAGCTCTCCTG
TGTAAGAATGCCAATGGCTTTTCAGGTGCCCCTGCAGAACCAGACCTCAGCCCCAGGGCA
GCTGCCGAAGCCCTGATGCAGGTTTGTGAGCATGAGACCACCCAACCAGATATGTCTTCT
CCTGGTGTGTTCAATGAAGTGCAGAAAGCACCTGCCACATTCAACTCTCCCGGCAATCCC
CAGCTGCCAGGGAGCAGCCAGCCCGCAGCATCAGCCCCGAGTTCTGCAGCAGGAAGGGAT
CTTATACACACACCATTGACAATGCCCGCCAATCAGCACACCTGCCAGTCCATCCCAGGT
GATCAGCCCAATGCCATCACCTCATCCATGCCTGAAGATTCCCTGATGAGATCACAGAGA
ACCTCAAATAGAGAGCAACCTGAGAAACCAAGTTGTCCTGTGGGAGGCGTCCTCAGTAGC
AGCAAAGATCAGGTGTCCTGTGAGTTTCCTTCTCCAGAAACAATCCAGGGAACAGTGCAG
ACTCCAGTGACAGCAGCCAGGGTGGTCAGTCACTCATCCTCTCCTGTAGGTGGACCTGAA
GGGGAAAGGCAGGGAGCCATCTGTGACTCTGAAATGAGGTCCTGTAAACCTCTAACTAGA
GAATCTGGATGTTCAGAGAACAAGCAGCCCTCTGTCACTGCCTCGGGCCCCCAAGGCACA
ACTTCTGTGACACCTCAACCAACCCCCCTCACTAGCGAACCTTCGGCATGTCCCCCAGGT
CCAGAGAAGGTGCCGCTGCCAGCACAGCGTCAGATGTCAAGGTTCAAAGAAGCCAGTACG
ATGACCAACCAAGCTGAAAGTGAAATCAAGGAAGTTCCCAGCAGGGCTTGGCAAGATGCG
GAGGTGCAGGCAGTGGCGAGTGTCGAGAGCAGATCCGTCTCCACCAGCCCCAGTATCCTC
ACTGCATTTCTGAAGGAAAGCCGTGCTCCTGAGCATTTTGAACAAGAGCAGCTGCGTGTC
ATTTGCCACAGCAGTGGGAGCCACACACTGGAGCTCTCTGACAGCACGCTAGCCCCCCAG
GAGTCCAGCCAGTGCCCTGGCATCATGCCACAGGTGCACATTCAGGCAGCTGCAGCTGAG
TCTACAGCTTTCCAACGGGAAAATAAACTTGCGAGCCTACCAGGTGGGGTCCTTAAAACC
TCATCAATCAATTTGGTCTCCAGTAATGCCCAGCATACGTGTAAAGAAGATGGGAGGTTA
GCAGGAATGACTCCAGTGAGGGAAGAGTCAACTGCTAAAAAGCTCGCAGGTACTAATTCT
AGCTCCCTGAAAGCTACCGCCATTGACCAGATTTCTATCAGTGCATGCAGTCAAGCTGAA
ACAAGTTATGGATTGGGGAAATTTGAAACCAGGCCATCTGAGTTTGCAGAGAAAACGACA
AACGGCCACAAAACAGACCCAGATTGCAAACTATCTGACTCTTGTGGCTCTATCAGCAAA
GCTGATCATTCTGGGAGCTTGGATCCCACTAATAAAGGAGATGCAAGGGAAAAGAAGCCT
GCATCTCCTCAGGTAGTAAAAGAAAAAGAGTCTACTGGCACTGATACCTCGGATGCCAAA
ACCCTACTGCTCAATCCTAAATCCCAAGAAAGTGGAGGCACAGAATCAGCTGCTAATCCT
ACACCCTCCCCAATTAGGAAGAACCAGGAGAGCACCTTAGAAGAAAACAGACAGACCAAG
ACAGCCACCAGCCTGAGCCTGCCATCTGATCCCATGGGTGACTCCAGCCCAGGTTCTGGC
AAGAAGACCCCATCTCGCTCCGTCAAAGCCAGCCCACGCAGGCCCAGCCGCGTCAGCGAG
TTCCTCAAGGAGCAAAAGTTAAATGTGACAGCAGCTGCTGCTCAGGTAGGACTCACTCCA
GGAGATAAGAAAAAGCAGCTTGGCGCAGACTCCAAGCTCCAGCTGAAACAGTCCAAGCGT
GTCAGGGACGTCGTGTGGGATGAGCAGGGAATGACCTGGGAAGTGTATGGTGCATCCTTG
GACGCAGAGTCCCTGGGAATCGCGATCCAGAACCATTTGCAAAGACAAATCAGGGAACAT
GAGAAATTAATCAAAACTCAAAATAGCCAGACCCGGAGATCCATTTCCTCAGATACTTCT
TCAAATAAGAAGCTCAGAGGAAGGCAGCACAGTGTTTTCCAGTCCATGCTGCAGAACTTC
CGACGCCCCAACTGCTGCGTCCGTCCTGCCCCGTCTTCTGTGTTAGAT

ORF Protein

Cloned ORF protein sequence for pFN21AA2027 Download

>KIAA2027  776 aa
MGTVPDPLRSAKTSLIAASGKEDDLGEPQAASPRHRPALLCKNANGFSGAPAEPDLSPRA
AAEALMQVCEHETTQPDMSSPGVFNEVQKAPATFNSPGNPQLPGSSQPAASAPSSAAGRD
LIHTPLTMPANQHTCQSIPGDQPNAITSSMPEDSLMRSQRTSNREQPEKPSCPVGGVLSS
SKDQVSCEFPSPETIQGTVQTPVTAARVVSHSSSPVGGPEGERQGAICDSEMRSCKPLTR
ESGCSENKQPSVTASGPQGTTSVTPQPTPLTSEPSACPPGPEKVPLPAQRQMSRFKEAST
MTNQAESEIKEVPSRAWQDAEVQAVASVESRSVSTSPSILTAFLKESRAPEHFEQEQLRV
ICHSSGSHTLELSDSTLAPQESSQCPGIMPQVHIQAAAAESTAFQRENKLASLPGGVLKT
SSINLVSSNAQHTCKEDGRLAGMTPVREESTAKKLAGTNSSSLKATAIDQISISACSQAE
TSYGLGKFETRPSEFAEKTTNGHKTDPDCKLSDSCGSISKADHSGSLDPTNKGDAREKKP
ASPQVVKEKESTGTDTSDAKTLLLNPKSQESGGTESAANPTPSPIRKNQESTLEENRQTK
TATSLSLPSDPMGDSSPGSGKKTPSRSVKASPRRPSRVSEFLKEQKLNVTAAAAQVGLTP
GDKKKQLGADSKLQLKQSKRVRDVVWDEQGMTWEVYGASLDAESLGIAIQNHLQRQIREH
EKLIKTQNSQTRRSISSDTSSNKKLRGRQHSVFQSMLQNFRRPNCCVRPAPSSVLD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA2027 Download

>pFN21AA2027  7033 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGACTGTACCTGACCCTCTGAGATCAGCTAA
AACTTCCCTGATTGCAGCTTCCGGAAAAGAAGACGATCTAGGAGAGCCACAGGCTGCCTC
ACCTCGGCATCGACCAGCTCTCCTGTGTAAGAATGCCAATGGCTTTTCAGGTGCCCCTGC
AGAACCAGACCTCAGCCCCAGGGCAGCTGCCGAAGCCCTGATGCAGGTTTGTGAGCATGA
GACCACCCAACCAGATATGTCTTCTCCTGGTGTGTTCAATGAAGTGCAGAAAGCACCTGC
CACATTCAACTCTCCCGGCAATCCCCAGCTGCCAGGGAGCAGCCAGCCCGCAGCATCAGC
CCCGAGTTCTGCAGCAGGAAGGGATCTTATACACACACCATTGACAATGCCCGCCAATCA
GCACACCTGCCAGTCCATCCCAGGTGATCAGCCCAATGCCATCACCTCATCCATGCCTGA
AGATTCCCTGATGAGATCACAGAGAACCTCAAATAGAGAGCAACCTGAGAAACCAAGTTG
TCCTGTGGGAGGCGTCCTCAGTAGCAGCAAAGATCAGGTGTCCTGTGAGTTTCCTTCTCC
AGAAACAATCCAGGGAACAGTGCAGACTCCAGTGACAGCAGCCAGGGTGGTCAGTCACTC
ATCCTCTCCTGTAGGTGGACCTGAAGGGGAAAGGCAGGGAGCCATCTGTGACTCTGAAAT
GAGGTCCTGTAAACCTCTAACTAGAGAATCTGGATGTTCAGAGAACAAGCAGCCCTCTGT
CACTGCCTCGGGCCCCCAAGGCACAACTTCTGTGACACCTCAACCAACCCCCCTCACTAG
CGAACCTTCGGCATGTCCCCCAGGTCCAGAGAAGGTGCCGCTGCCAGCACAGCGTCAGAT
GTCAAGGTTCAAAGAAGCCAGTACGATGACCAACCAAGCTGAAAGTGAAATCAAGGAAGT
TCCCAGCAGGGCTTGGCAAGATGCGGAGGTGCAGGCAGTGGCGAGTGTCGAGAGCAGATC
CGTCTCCACCAGCCCCAGTATCCTCACTGCATTTCTGAAGGAAAGCCGTGCTCCTGAGCA
TTTTGAACAAGAGCAGCTGCGTGTCATTTGCCACAGCAGTGGGAGCCACACACTGGAGCT
CTCTGACAGCACGCTAGCCCCCCAGGAGTCCAGCCAGTGCCCTGGCATCATGCCACAGGT
GCACATTCAGGCAGCTGCAGCTGAGTCTACAGCTTTCCAACGGGAAAATAAACTTGCGAG
CCTACCAGGTGGGGTCCTTAAAACCTCATCAATCAATTTGGTCTCCAGTAATGCCCAGCA
TACGTGTAAAGAAGATGGGAGGTTAGCAGGAATGACTCCAGTGAGGGAAGAGTCAACTGC
TAAAAAGCTCGCAGGTACTAATTCTAGCTCCCTGAAAGCTACCGCCATTGACCAGATTTC
TATCAGTGCATGCAGTCAAGCTGAAACAAGTTATGGATTGGGGAAATTTGAAACCAGGCC
ATCTGAGTTTGCAGAGAAAACGACAAACGGCCACAAAACAGACCCAGATTGCAAACTATC
TGACTCTTGTGGCTCTATCAGCAAAGCTGATCATTCTGGGAGCTTGGATCCCACTAATAA
AGGAGATGCAAGGGAAAAGAAGCCTGCATCTCCTCAGGTAGTAAAAGAAAAAGAGTCTAC
TGGCACTGATACCTCGGATGCCAAAACCCTACTGCTCAATCCTAAATCCCAAGAAAGTGG
AGGCACAGAATCAGCTGCTAATCCTACACCCTCCCCAATTAGGAAGAACCAGGAGAGCAC
CTTAGAAGAAAACAGACAGACCAAGACAGCCACCAGCCTGAGCCTGCCATCTGATCCCAT
GGGTGACTCCAGCCCAGGTTCTGGCAAGAAGACCCCATCTCGCTCCGTCAAAGCCAGCCC
ACGCAGGCCCAGCCGCGTCAGCGAGTTCCTCAAGGAGCAAAAGTTAAATGTGACAGCAGC
TGCTGCTCAGGTAGGACTCACTCCAGGAGATAAGAAAAAGCAGCTTGGCGCAGACTCCAA
GCTCCAGCTGAAACAGTCCAAGCGTGTCAGGGACGTCGTGTGGGATGAGCAGGGAATGAC
CTGGGAAGTGTATGGTGCATCCTTGGACGCAGAGTCCCTGGGAATCGCGATCCAGAACCA
TTTGCAAAGACAAATCAGGGAACATGAGAAATTAATCAAAACTCAAAATAGCCAGACCCG
GAGATCCATTTCCTCAGATACTTCTTCAAATAAGAAGCTCAGAGGAAGGCAGCACAGTGT
TTTCCAGTCCATGCTGCAGAACTTCCGACGCCCCAACTGCTGCGTCCGTCCTGCCCCGTC
TTCTGTGTTAGATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT