RHOT2 ORF clone FHC11153

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11153

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Clone info.

Species

human

Clone Name

pFN21AE4166 Sequence (with vector) Restriction Map

Product ID

FHC11153

Flexi Clone PID

FXC11153

ORF Sequence

DNA sequence (1854 bp)
Protein sequence (618 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4166

Symbol

RHOT2

Alias : ARHT2, C16orf39, MIRO-2, MIRO2, RASL

Description

ras homolog family member T2

Original Clone ID

cp94166

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1854 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 618 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10417.1 0 100.0 RHOT2 CCDS32612.1 5.1e-138 60.1 RHOT1 CCDS74031.1 3.3e-132 59.6 RHOT1 CCDS32611.1 4e-132 61.3 RHOT1 CCDS74030.1 1.5e-131 57.2 RHOT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 5 26 PR00449 Ras GTPase IPR001806 109 122 PR00449 Ras GTPase IPR001806 144 166 PR00449 Ras GTPase HMMPfam IPR013753 6 166 PF00071 Ras IPR013567 219 306 PF08356 EF hand associated IPR013566 341 412 PF08355 EF hand associated IPR013684 420 527 PF08477 Mitochondrial Rho-like HMMSmart IPR003577 2 169 SM00173 Ras small GTPase IPR003579 5 169 SM00175 Ras small GTPase IPR003578 7 169 SM00174 Small GTPase ProfileScan IPR020860 1 142 PS51423 MIRO IPR018249 184 219 PS50222 EF-HAND 2 IPR018249 304 339 PS50222 EF-HAND 2 IPR020860 410 618 PS51423 MIRO
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 592 RGLLGVVGAAVAAVLSFSLYRVL 614 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_620124 618 613889 100.0 100.0 NP_001339204 619 613889 99.8 100.0 NP_001339206 600 613889 97.1 100.0 NP_001339205 601 613889 96.9 100.0 XP_024306246 790 613889 99.8 85.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4166 Download

>KIEE4166  1854 bp
ATGAGGCGGGACGTGCGCATCCTGTTACTGGGCGAGGCCCAGGTGGGGAAGACGTCGCTG
ATCCTGTCCCTGGTGGGCGAGGAGTTCCCCGAGGAGGTCCCTCCCCGCGCGGAGGAGATC
ACGATCCCCGCGGACGTCACCCCGGAGAAGGTGCCCACCCACATCGTGGACTACTCAGAA
GCCGAGCAGACGGACGAGGAGCTGCGGGAGGAGATCCACAAGGCAAACGTGGTGTGTGTG
GTGTATGACGTCTCTGAGGAGGCCACCATTGAGAAGATTCGAACTAAGTGGATCCCACTG
GTGAATGGGGGGACCACGCAGGGGCCCAGGGTGCCCATCATCCTAGTGGGCAACAAGTCA
GACCTGCGGTCGGGGAGCTCCATGGAGGCCGTGCTCCCCATCATGAGCCAGTTTCCCGAG
ATTGAGACCTGCGTGGAGTGTTCGGCCAAGAACCTGAGGAACATCTCAGAGCTGTTCTAC
TACGCCCAGAAGGCCGTCCTGCATCCCACAGCCCCCCTCTATGACCCTGAGGCCAAGCAG
TTGAGGCCCGCGTGCGCCCAGGCGCTGACGCGCATCTTCAGGCTCTCAGATCAGGACCTG
GACCAGGCGCTCAGTGACGAAGAGCTCAACGCTTTCCAGAAATCCTGCTTTGGGCACCCC
CTGGCCCCGCAGGCCCTGGAGGACGTGAAGACGGTGGTGTGCAGGAACGTGGCGGGCGGC
GTGCGGGAGGACCGGCTGACCCTGGATGGTTTCCTCTTCCTGAACACGCTCTTCATCCAG
CGCGGCCGGCACGAGACCACCTGGACCATCCTGCGGCGCTTCGGCTACAGCGATGCCCTG
GAGCTGACTGCGGACTATCTCTCCCCTCTGATCCACGTGCCCCCCGGCTGCAGCACGGAG
CTCAACCACCTTGGCTACCAGTTTGTGCAGAGAGTGTTTGAGAAGCACGACCAGGACCGC
GACGGCGCCCTCTCGCCCGTGGAGCTGCAAAGCCTTTTCAGTGTGTTCCCAGCAGCGCCC
TGGGGCCCCGAGCTCCCACGCACAGTCCGCACAGAGGCCGGCCGGTTGCCCCTGCACGGA
TACCTCTGCCAGTGGACCCTGGTGACCTACCTGGACGTCCGGAGCTGCCTTGGACACCTA
GGCTACCTGGGCTACCCCACCCTCTGTGAGCAGGACCAGGCCCATGCCATCACAGTCACT
CGTGAGAAGAGGCTGGACCAGGAGAAGGGACAGACGCAGCGGAGCGTCCTCCTGTGCAAG
GTGGTAGGGGCCCGTGGAGTGGGCAAGTCTGCCTTCCTGCAGGCCTTTCTCGGCCGCGGC
CTGGGGCACCAGGACACGAGGGAGCAGCCTCCCGGCTACGCCATCGACACGGTGCAGGTC
AATGGACAGGAGAAGTACTTGATCCTCTGTGAGGTGGGCACAGATGGTCTGCTGGCCACA
TCGCTGGACGCCACCTGTGACGTTGCCTGCTTGATGTTTGATGGCAGTGACCCAAAGTCC
TTTGCACATTGTGCCAGCGTCTACAAGCACCATTACATGGACGGGCAGACCCCCTGCCTC
TTTGTCTCCTCCAAGGCCGACCTGCCCGAAGGTGTCGCGGTGTCTGGCCCATCACCGGCC
GAGTTTTGCCGCAAGCACCGGCTACCCGCTCCCGTGCCGTTCTCCTGTGCTGGCCCAGCC
GAGCCCAGCACCACCATCTTCACCCAGCTCGCCACCATGGCCGCCTTCCCACATTTGGTC
CACGCAGAGCTGCATCCCTCTTCCTTCTGGCTCCGGGGGCTGCTGGGGGTTGTCGGGGCC
GCCGTGGCCGCAGTCCTCAGCTTCTCACTCTACAGGGTCCTGGTGAAGAGCCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE4166 Download

>KIEE4166  618 aa
MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSE
AEQTDEELREEIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKS
DLRSGSSMEAVLPIMSQFPEIETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQ
LRPACAQALTRIFRLSDQDLDQALSDEELNAFQKSCFGHPLAPQALEDVKTVVCRNVAGG
VREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDALELTADYLSPLIHVPPGCSTE
LNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVRTEAGRLPLHG
YLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCK
VVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLAT
SLDATCDVACLMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPA
EFCRKHRLPAPVPFSCAGPAEPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGA
AVAAVLSFSLYRVLVKSQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4166 Download

>pFN21AE4166  6559 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGCGGGACGTGCGCATCCTGTTACTGGGCGA
GGCCCAGGTGGGGAAGACGTCGCTGATCCTGTCCCTGGTGGGCGAGGAGTTCCCCGAGGA
GGTCCCTCCCCGCGCGGAGGAGATCACGATCCCCGCGGACGTCACCCCGGAGAAGGTGCC
CACCCACATCGTGGACTACTCAGAAGCCGAGCAGACGGACGAGGAGCTGCGGGAGGAGAT
CCACAAGGCAAACGTGGTGTGTGTGGTGTATGACGTCTCTGAGGAGGCCACCATTGAGAA
GATTCGAACTAAGTGGATCCCACTGGTGAATGGGGGGACCACGCAGGGGCCCAGGGTGCC
CATCATCCTAGTGGGCAACAAGTCAGACCTGCGGTCGGGGAGCTCCATGGAGGCCGTGCT
CCCCATCATGAGCCAGTTTCCCGAGATTGAGACCTGCGTGGAGTGTTCGGCCAAGAACCT
GAGGAACATCTCAGAGCTGTTCTACTACGCCCAGAAGGCCGTCCTGCATCCCACAGCCCC
CCTCTATGACCCTGAGGCCAAGCAGTTGAGGCCCGCGTGCGCCCAGGCGCTGACGCGCAT
CTTCAGGCTCTCAGATCAGGACCTGGACCAGGCGCTCAGTGACGAAGAGCTCAACGCTTT
CCAGAAATCCTGCTTTGGGCACCCCCTGGCCCCGCAGGCCCTGGAGGACGTGAAGACGGT
GGTGTGCAGGAACGTGGCGGGCGGCGTGCGGGAGGACCGGCTGACCCTGGATGGTTTCCT
CTTCCTGAACACGCTCTTCATCCAGCGCGGCCGGCACGAGACCACCTGGACCATCCTGCG
GCGCTTCGGCTACAGCGATGCCCTGGAGCTGACTGCGGACTATCTCTCCCCTCTGATCCA
CGTGCCCCCCGGCTGCAGCACGGAGCTCAACCACCTTGGCTACCAGTTTGTGCAGAGAGT
GTTTGAGAAGCACGACCAGGACCGCGACGGCGCCCTCTCGCCCGTGGAGCTGCAAAGCCT
TTTCAGTGTGTTCCCAGCAGCGCCCTGGGGCCCCGAGCTCCCACGCACAGTCCGCACAGA
GGCCGGCCGGTTGCCCCTGCACGGATACCTCTGCCAGTGGACCCTGGTGACCTACCTGGA
CGTCCGGAGCTGCCTTGGACACCTAGGCTACCTGGGCTACCCCACCCTCTGTGAGCAGGA
CCAGGCCCATGCCATCACAGTCACTCGTGAGAAGAGGCTGGACCAGGAGAAGGGACAGAC
GCAGCGGAGCGTCCTCCTGTGCAAGGTGGTAGGGGCCCGTGGAGTGGGCAAGTCTGCCTT
CCTGCAGGCCTTTCTCGGCCGCGGCCTGGGGCACCAGGACACGAGGGAGCAGCCTCCCGG
CTACGCCATCGACACGGTGCAGGTCAATGGACAGGAGAAGTACTTGATCCTCTGTGAGGT
GGGCACAGATGGTCTGCTGGCCACATCGCTGGACGCCACCTGTGACGTTGCCTGCTTGAT
GTTTGATGGCAGTGACCCAAAGTCCTTTGCACATTGTGCCAGCGTCTACAAGCACCATTA
CATGGACGGGCAGACCCCCTGCCTCTTTGTCTCCTCCAAGGCCGACCTGCCCGAAGGTGT
CGCGGTGTCTGGCCCATCACCGGCCGAGTTTTGCCGCAAGCACCGGCTACCCGCTCCCGT
GCCGTTCTCCTGTGCTGGCCCAGCCGAGCCCAGCACCACCATCTTCACCCAGCTCGCCAC
CATGGCCGCCTTCCCACATTTGGTCCACGCAGAGCTGCATCCCTCTTCCTTCTGGCTCCG
GGGGCTGCTGGGGGTTGTCGGGGCCGCCGTGGCCGCAGTCCTCAGCTTCTCACTCTACAG
GGTCCTGGTGAAGAGCCAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT