MAPK15 ORF clone FHC10717

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Halotag ORF Clone FHC10717

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Clone info.

Species

human

Clone Name

pFN21AE0975 Sequence (with vector) Restriction Map

Product ID

FHC10717

Flexi Clone PID

FXC10717

ORF Sequence

DNA sequence (1632 bp)
Protein sequence (544 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB587543]

Gene

KIEE0975

Symbol

MAPK15

Alias : ERK7, ERK8

Description

mitogen-activated protein kinase 15

Original Clone ID

cp01475

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1632 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 544 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6409.2 1.2e-104 100.0 MAPK15 CCDS42149.1 1.6e-20 45.2 MAPK3 CCDS10672.1 1.7e-20 45.2 MAPK3 CCDS11206.1 2.2e-20 37.3 MAPK7 CCDS13795.1 5.9e-20 44.7 MAPK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR017442 15 304 PF00069 Serine/threonine-protein kinase-like domain HMMSmart IPR002290 13 304 SM00220 Serine/threonine-protein kinase domain IPR020635 13 307 SM00219 Tyrosine-protein kinase ProfileScan IPR000719 13 304 PS50011 Protein kinase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0975 Download

>KIEE0975  1632 bp
ATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAGGCGGCAGCTCGGG
CAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACCGGAGGACTGGTGAGGTCGTGGCC
ATCAAGAAAATCTTTGATGCTTTTAGGGATAAGACAGATGCCCAGAGAACATTCCGGGAA
ATCACGCTCCTCCAGGAGTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATC
CGGGCAGAGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTGAAC
GCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTCCATCTTCTACCAG
CTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACGTTGTGCACCGGGACCAGAAGCCG
TCCAATGTGCTCCTGGATGCCAACTGCACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGC
TCCCTGGGCGACCTCCCTGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACA
CGCTGGTACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTGGAC
ATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACCCCTGTTCCCCGGC
ACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGACCATCCCACCGCCATCTGAGGAG
GACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGG
CCACGACAGACGCTGGATGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTC
CTTAGGCGACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTGCAG
CACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACGAGAGGCAGATGTG
CGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGCCTGAGTACCGCAGCCGCGTCTAT
CAGATGATCCTGGAGTGTGGAGGCAGCAGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGT
GTCTCCCCAAGCCAGGCACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGG
ACACCTGTGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCCGAG
CACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGCTCCCCTGCTCCAA
ACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGGCGAAGGAAGCGCCCCCCTTGACA
CTCTCGCTGGTGAAGCCAAGCGGGAGGGGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCG
GCTCAGGTGGCCAACCAGGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGG
GTGGCCAGCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGGAGG
ATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGCTTTGCTTGGAGGC
TACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCACTGGGCCACCTGCCCCTGCTGGAG
GGGCACCATGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE0975 Download

>KIEE0975  544 aa
MCTVVDPRIVRRYLLRRQLGQGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFRE
ITLLQEFGDHPNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQ
LLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEYVAT
RWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQLELILETIPPPSEE
DLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEALDLLRRLLVFAPDKRLSATQALQ
HPYVQRFHCPSDEWAREADVRPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEG
VSPSQAHLHKPRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQ
TALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIRGDWNRGGGVR
VASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGTVCHSALGHLPLLE
GHHV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0975 Download

>pFN21AE0975  6337 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTGCACCGTAGTGGACCCTCGCATTGTCCGGAG
ATACCTACTCAGGCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGA
CCGGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGGGATAAGAC
AGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGAGTTTGGGGACCATCCCAA
CATCATCAGCCTCCTTGACGTGATCCGGGCAGAGAACGACAGGGACATTTACCTGGTGTT
TGAGTTTATGGACACTGACCTGAACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGT
CCACGTGCGCTCCATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCA
CGTTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGCACAGTGAA
GCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCCTGAGGGGCCTGAGGACCA
GGCCGTGACAGAGTACGTGGCCACACGCTGGTACCGAGCACCGGAGGTGCTGCTCTCTTC
GCACCGATACACCCTTGGGGTGGACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCT
GCGGGGGAGACCCCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGA
GACCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGCCGTGCCTC
TGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGATGCCCTCCTACCGCCAGA
CACCTCCCCAGAGGCCTTGGACCTCCTTAGGCGACTCCTGGTGTTCGCCCCGGACAAGCG
GTTAAGCGCGACCCAGGCACTGCAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGA
CGAGTGGGCACGAGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGT
GCCTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGCAGCGGCAC
CTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGCACACCTGCACAAACCCAG
AGCCGACCCTCAGCTGCCTTCTAGGACACCTGTGCAGGGTCCCAGACCCAGGCCCCAGAG
CAGCCCAGGCCATGACCCTGCCGAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAG
GCAGAACTCCGCTCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGG
GGCGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAAGCGGGAGGGGAGCTGC
GCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCAGGCCCTGATCCGGGGTGA
CTGGAACCGGGGCGGTGGGGTGAGGGTGGCCAGCGTACAACAGGTCCCTCCCCGGCTTCC
TCCGGAGGCCCGGCCCGGCCGGAGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGG
GGGTGCCAGGGCTTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGC
ACTGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT