SLC1A2 ORF clone FHC10502

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10502

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Clone info.

Species

human

Clone Name

pFN21AB4461 Sequence (with vector) Restriction Map

Product ID

FHC10502

Flexi Clone PID

FXC10502

ORF Sequence

DNA sequence (1722 bp)
Protein sequence (574 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527302]

Gene

KIBB4461

Symbol

SLC1A2

Alias : DEE41, EAAT2, EIEE41, GLT-1, HBGT

Description

solute carrier family 1 (glial high affinity glutamate transporter), member 2, transcript variant 1

Original Clone ID

pj02467

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1722 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 574 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31459.1 0 99.8 SLC1A2 CCDS55756.1 0 99.8 SLC1A2 CCDS3919.1 2.4e-110 53.8 SLC1A3 CCDS6452.1 2.2e-109 55.1 SLC1A1 CCDS78004.1 4.9e-100 52.8 SLC1A3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001991 44 64 PR00173 Sodium:dicarboxylate symporter IPR001991 88 108 PR00173 Sodium:dicarboxylate symporter IPR001991 117 142 PR00173 Sodium:dicarboxylate symporter IPR001991 237 259 PR00173 Sodium:dicarboxylate symporter IPR001991 284 303 PR00173 Sodium:dicarboxylate symporter IPR001991 321 340 PR00173 Sodium:dicarboxylate symporter IPR001991 388 414 PR00173 Sodium:dicarboxylate symporter IPR001991 423 442 PR00173 Sodium:dicarboxylate symporter IPR001991 450 470 PR00173 Sodium:dicarboxylate symporter HMMPfam IPR001991 46 495 PF00375 Sodium:dicarboxylate symporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 40 KLGKNLLLTLTVFGVILGAVCGG 62 PRIMARY 23 2 80 FPGDILMRMLKMLIFPLIISSLI 102 SECONDARY 23 3 124 YMSTTIIAAVLGVILVLAIHPGN 146 PRIMARY 23 4 237 KDGMNVLGLIGFFIAFGIAMGKM 259 SECONDARY 23 5 263 AKLMVDFFNILNEIVMKLVIMIM 285 PRIMARY 23 6 289 PLGIACLICGKIIAIKDLEVVAR 311 PRIMARY 23 7 316 YMVTVIIGLIIHGGIFLPLIYFV 338 PRIMARY 23 8 347 FFAGIFQAWITALGTASSAGTLP 369 SECONDARY 23 9 404 YEAVAAIFIAQMNGVVLDGGQIV 426 SECONDARY 23 10 435 ASVGAASIPSAGLVTMLLILTAV 457 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004162 574 600300,617105 99.8 100.0 XP_011518587 570 600300,617105 99.8 99.1 XP_005253124 571 600300,617105 99.8 99.0 XP_016873625 579 600300,617105 99.8 99.0 NP_001182657 565 600300,617105 99.8 98.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB4461 Download

>KIBB4461  1722 bp
ATGGCATCTACGGAAGGTGCCAACAATATGCCCAAGCAGGTGGAAGTGCGAATGCACGAC
AGTCATCTTGGCTCAGAGGAACCCAAGCACCGGCACCTGGGCCTGCGCCTGTGTGACAAG
CTGGGGAAGAATCTGCTGCTCACCCTGACGGTGTTTGGTGTCATCCTGGGAGCAGTGTGT
GGAGGGCTTCTTCGCTTGGCATCTCCCATCCACCCTGATGTGGTTATGTTAATAGCCTTC
CCAGGGGATATACTCATGAGGATGCTAAAAATGCTCATTTTCCCTCTAATCATCTCCAGC
TTAATCACAGGGTTGTCAGGCCTGGATGCTAAGGCTAGTGGCCGCTTGGGCACGAGAGCC
ATGGTGTATTACATGTCCACGACCATCATTGCTGCAGTACTGGGGGTCATTCTGGTCTTG
GCTATCCATCCAGGCAATCCCAAGCTCAAGAAGCAGCTGGGGCCTGGGAAGAAGAATGAT
GAAGTGTCCAGCCTGGATGCCTTCCTGGACCTTATTCGAAATCTCTTCCCTGAAAACCTT
GTCCAAGCCTGCTTTCAACAGATTCAAACAGTGACGAAGAAAGTCCTGGTTGCACCACCG
CCGGACGAGGAGGCCAACGCAACCAGCGCTGTTGTCTCTCTGTTGAACGAGACTGTGACT
GAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAAGGGCCTGGAGTTCAAGGATGGGATG
AACGTCTTAGGTCTGATAGGGTTTTTCATTGCTTTTGGCATCGCTATGGGGAAGATGGGA
GATCAGGCCAAGCTGATGGTGGATTTCTTCAACATTTTGAATGAGATTGTAATGAAGTTA
GTGATCATGATCATGTGGTACTCTCCCCTGGGTATCGCCTGCCTGATCTGTGGAAAGATC
ATTGCAATCAAGGACTTAGAAGTGGTTGCTAGGCAACTGGGGATGTACATGGTAACAGTG
ATCATAGGCCTCATCATCCACGGGGGCATCTTTCTCCCCTTGATTTACTTTGTAGTGACC
AGGAAAAACCCCTTCTCCTTTTTTGCTGGCATTTTCCAAGCTTGGATCACTGCCCTGGGC
ACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTTTCGTTGCCTGGAAGAAAATCTGGGG
ATTGATAAGCGTGTGACTAGATTCGTCCTTCCTGTTGGAGCAACCATTAACATGGATGGT
ACAGCCCTTTATGAAGCGGTAGCCGCCATCTTTATAGCCCAAATGAATGGTGTTGTCCTG
GATGGAGGACAGATTGTGACTGTAAGCCTCACAGCCACCCTGGCAAGCGTCGGCGCGGCC
AGTATCCCCAGTGCCGGGCTGGTCACCATGCTCCTCATTCTGACAGCCGTGGGCCTGCCA
ACAGAGGACATCAGCCTGCTGGTGGCTGTGGACTGGCTGCTGGACAGGATGAGAACTTCA
GTCAATGTTGTGGGTGACTCTTTTGGGGCTGGGATAGTCTATCACCTCTCCAAGTCTGAG
CTGGATACCATTGACTCCCAGCATCGAGTGCATGAAGATATTGAAATGACCAAGACTCAA
TCCATTTATGATGACATGAAGAACCACAGGGAAAGCAACTCTAATCAATGTGTCTATGCT
GCACACAACTCTGTCATAGTAGATGAATGCAAGGTAACTCTGGCAGCCAATGGAAAGTCA
GCCGACTGCAGTGTTGAGGAAGAACCTTGGAAACGTGAGAAA

ORF Protein

Cloned ORF protein sequence for pFN21AB4461 Download

>KIBB4461  574 aa
MASTEGANNMPKQVEVRMHDSHLGSEEPKHRHLGLRLCDKLGKNLLLTLTVFGVILGAVC
GGLLRLASPIHPDVVMLIAFPGDILMRMLKMLIFPLIISSLITGLSGLDAKASGRLGTRA
MVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENL
VQACFQQIQTVTKKVLVAPPPDEEANATSAVVSLLNETVTEVPEETKMVIKKGLEFKDGM
NVLGLIGFFIAFGIAMGKMGDQAKLMVDFFNILNEIVMKLVIMIMWYSPLGIACLICGKI
IAIKDLEVVARQLGMYMVTVIIGLIIHGGIFLPLIYFVVTRKNPFSFFAGIFQAWITALG
TASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVL
DGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTS
VNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYA
AHNSVIVDECKVTLAANGKSADCSVEEEPWKREK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB4461 Download

>pFN21AB4461  6427 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCATCTACGGAAGGTGCCAACAATATGCCCAA
GCAGGTGGAAGTGCGAATGCACGACAGTCATCTTGGCTCAGAGGAACCCAAGCACCGGCA
CCTGGGCCTGCGCCTGTGTGACAAGCTGGGGAAGAATCTGCTGCTCACCCTGACGGTGTT
TGGTGTCATCCTGGGAGCAGTGTGTGGAGGGCTTCTTCGCTTGGCATCTCCCATCCACCC
TGATGTGGTTATGTTAATAGCCTTCCCAGGGGATATACTCATGAGGATGCTAAAAATGCT
CATTTTCCCTCTAATCATCTCCAGCTTAATCACAGGGTTGTCAGGCCTGGATGCTAAGGC
TAGTGGCCGCTTGGGCACGAGAGCCATGGTGTATTACATGTCCACGACCATCATTGCTGC
AGTACTGGGGGTCATTCTGGTCTTGGCTATCCATCCAGGCAATCCCAAGCTCAAGAAGCA
GCTGGGGCCTGGGAAGAAGAATGATGAAGTGTCCAGCCTGGATGCCTTCCTGGACCTTAT
TCGAAATCTCTTCCCTGAAAACCTTGTCCAAGCCTGCTTTCAACAGATTCAAACAGTGAC
GAAGAAAGTCCTGGTTGCACCACCGCCGGACGAGGAGGCCAACGCAACCAGCGCTGTTGT
CTCTCTGTTGAACGAGACTGTGACTGAGGTGCCGGAGGAGACTAAGATGGTTATCAAGAA
GGGCCTGGAGTTCAAGGATGGGATGAACGTCTTAGGTCTGATAGGGTTTTTCATTGCTTT
TGGCATCGCTATGGGGAAGATGGGAGATCAGGCCAAGCTGATGGTGGATTTCTTCAACAT
TTTGAATGAGATTGTAATGAAGTTAGTGATCATGATCATGTGGTACTCTCCCCTGGGTAT
CGCCTGCCTGATCTGTGGAAAGATCATTGCAATCAAGGACTTAGAAGTGGTTGCTAGGCA
ACTGGGGATGTACATGGTAACAGTGATCATAGGCCTCATCATCCACGGGGGCATCTTTCT
CCCCTTGATTTACTTTGTAGTGACCAGGAAAAACCCCTTCTCCTTTTTTGCTGGCATTTT
CCAAGCTTGGATCACTGCCCTGGGCACCGCTTCCAGTGCTGGAACTTTGCCTGTCACCTT
TCGTTGCCTGGAAGAAAATCTGGGGATTGATAAGCGTGTGACTAGATTCGTCCTTCCTGT
TGGAGCAACCATTAACATGGATGGTACAGCCCTTTATGAAGCGGTAGCCGCCATCTTTAT
AGCCCAAATGAATGGTGTTGTCCTGGATGGAGGACAGATTGTGACTGTAAGCCTCACAGC
CACCCTGGCAAGCGTCGGCGCGGCCAGTATCCCCAGTGCCGGGCTGGTCACCATGCTCCT
CATTCTGACAGCCGTGGGCCTGCCAACAGAGGACATCAGCCTGCTGGTGGCTGTGGACTG
GCTGCTGGACAGGATGAGAACTTCAGTCAATGTTGTGGGTGACTCTTTTGGGGCTGGGAT
AGTCTATCACCTCTCCAAGTCTGAGCTGGATACCATTGACTCCCAGCATCGAGTGCATGA
AGATATTGAAATGACCAAGACTCAATCCATTTATGATGACATGAAGAACCACAGGGAAAG
CAACTCTAATCAATGTGTCTATGCTGCACACAACTCTGTCATAGTAGATGAATGCAAGGT
AACTCTGGCAGCCAATGGAAAGTCAGCCGACTGCAGTGTTGAGGAAGAACCTTGGAAACG
TGAGAAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT