ACOT9 ORF clone FHC10397

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC10397

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Clone info.

Species

human

Clone Name

pFN21AB5521 Sequence (with vector) Restriction Map

Product ID

FHC10397

Flexi Clone PID

FXC10397

Original Clone PID

ORK10397

ORF Sequence

DNA sequence (1344 bp)
Protein sequence (448 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528567]

Gene

KIBB5521

Symbol

ACOT9

Alias : ACATE2, CGI-16, MT-ACT48, MTACT48

Description

acyl-CoA thioesterase 9, transcript variant 1

Original Clone ID

ef02192

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1344 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 448 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43924.1 1.1e-194 100.0 ACOT9 CCDS35216.1 5.6e-173 98.0 ACOT9 CCDS83460.1 1.3e-162 100.0 ACOT9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006683 318 383 PF03061 Thioesterase superfamily
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001032248 448 300862 100.0 100.0 NP_001028755 439 300862 98.0 100.0 NP_001317188 379 300862 100.0 84.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5521 Download

>KIBB5521  1344 bp
ATGAGGCGGGCAGCACTGCGGCTTTGTGCCTTGGGCAAAGGGCAGCTTACTCCTGGAAGA
GGACTGACTCAAGGACCCCAGAACCCCAAGAAACAGGGAATCTTCCACATTCATGAAGCA
TGTTCATCTATACATGTGAATCATGTTCGAGATAAGTTGCGGGAGATAGTAGGAGCATCC
ACAAACTGGAGAGACCATGTGAAGGCAATGGAAGAAAGGAAATTACTTCATAGTTTCTTG
GCTAAATCACAGGATGGACTGCCTCCTAGGAGAATGAAGGACAGTTATATTGAAGTTCTC
TTGCCTTTGGGCAGTGAGCCTGAATTACGAGAGAAATATTTGACTGTTCAAAACACCGTA
AGATTTGGCAGGATTCTTGAGGATCTTGACAGCTTGGGAGTTCTTATTTGTTACATGCAC
AACAAAATCCACTCCGCCAAGATGTCTCCTTTATCGATAGTTACAGCCCTGGTGGATAAG
ATTGATATGTGTAAGAAGAGCTTGAGCCCAGAACAGGACATTAAGTTCAGTGGCCATGTT
AGCTGGGTCGGGAAGACATCCATGGAAGTGAAGATGCAAATGTTCCAGTTACATGGTGAT
GAATTTTGTCCTGTTTTGGATGCAACATTTGTAATGGTGGCTCGTGATTCTGAAAATAAA
GGGCCGGCATTTGTAAATCCACTCATCCCTGAAAGCCCAGAGGAAGAGGAGCTCTTTAGA
CAAGGGGAATTGAACAAGGGGAGAAGAATTGCCTTCAGCTCCACGTCGTTACTGAAAATG
GCCCCCAGCGCTGAGGAGAGGACCACCATACATGAGATGTTTCTCAGCACACTGGATCCA
AAGACTATAAGTTTTCGGAGTCGAGTTTTACCCTCTAATGCAGTGTGGATGGAGAATTCA
AAACTGAAGAGTTTGGAAATTTGCCACCCTCAGGAGCGGAACATTTTCAATCGGATCTTT
GGTGGTTTCCTTATGAGGAAGGCATATGAACTTGCGTGGGCTACTGCTTGTAGCTTTGGT
GGTTCTCGACCGTTTGTGGTAGCAGTAGATGACATCATGTTTCAGAAACCTGTTGAGGTT
GGCTCATTGCTCTTTCTTTCTTCACAGGTATGCTTTACTCAGAATAATTATATTCAAGTC
AGAGTACACAGTGAAGTGGCCTCCCTGCAGGAGAAGCAGCATACAACCACCAATGTCTTT
CATTTCACGTTCATGTCGGAAAAAGAAGTGCCATTGGTTTTCCCAAAAACATATGGAGAG
TCCATGTTGTACTTAGATGGGCAGCGGCATTTCAACTCCATGAGTGGCCCAGCGACCTTG
AGAAAGGACTACCTTGTGGAGCCC

ORF Protein

Cloned ORF protein sequence for pFN21AB5521 Download

>KIBB5521  448 aa
MRRAALRLCALGKGQLTPGRGLTQGPQNPKKQGIFHIHEACSSIHVNHVRDKLREIVGAS
TNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTV
RFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHV
SWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKGPAFVNPLIPESPEEEELFR
QGELNKGRRIAFSSTSLLKMAPSAEERTTIHEMFLSTLDPKTISFRSRVLPSNAVWMENS
KLKSLEICHPQERNIFNRIFGGFLMRKAYELAWATACSFGGSRPFVVAVDDIMFQKPVEV
GSLLFLSSQVCFTQNNYIQVRVHSEVASLQEKQHTTTNVFHFTFMSEKEVPLVFPKTYGE
SMLYLDGQRHFNSMSGPATLRKDYLVEP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5521 Download

>pFN21AB5521  6049 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGGCGGGCAGCACTGCGGCTTTGTGCCTTGGG
CAAAGGGCAGCTTACTCCTGGAAGAGGACTGACTCAAGGACCCCAGAACCCCAAGAAACA
GGGAATCTTCCACATTCATGAAGCATGTTCATCTATACATGTGAATCATGTTCGAGATAA
GTTGCGGGAGATAGTAGGAGCATCCACAAACTGGAGAGACCATGTGAAGGCAATGGAAGA
AAGGAAATTACTTCATAGTTTCTTGGCTAAATCACAGGATGGACTGCCTCCTAGGAGAAT
GAAGGACAGTTATATTGAAGTTCTCTTGCCTTTGGGCAGTGAGCCTGAATTACGAGAGAA
ATATTTGACTGTTCAAAACACCGTAAGATTTGGCAGGATTCTTGAGGATCTTGACAGCTT
GGGAGTTCTTATTTGTTACATGCACAACAAAATCCACTCCGCCAAGATGTCTCCTTTATC
GATAGTTACAGCCCTGGTGGATAAGATTGATATGTGTAAGAAGAGCTTGAGCCCAGAACA
GGACATTAAGTTCAGTGGCCATGTTAGCTGGGTCGGGAAGACATCCATGGAAGTGAAGAT
GCAAATGTTCCAGTTACATGGTGATGAATTTTGTCCTGTTTTGGATGCAACATTTGTAAT
GGTGGCTCGTGATTCTGAAAATAAAGGGCCGGCATTTGTAAATCCACTCATCCCTGAAAG
CCCAGAGGAAGAGGAGCTCTTTAGACAAGGGGAATTGAACAAGGGGAGAAGAATTGCCTT
CAGCTCCACGTCGTTACTGAAAATGGCCCCCAGCGCTGAGGAGAGGACCACCATACATGA
GATGTTTCTCAGCACACTGGATCCAAAGACTATAAGTTTTCGGAGTCGAGTTTTACCCTC
TAATGCAGTGTGGATGGAGAATTCAAAACTGAAGAGTTTGGAAATTTGCCACCCTCAGGA
GCGGAACATTTTCAATCGGATCTTTGGTGGTTTCCTTATGAGGAAGGCATATGAACTTGC
GTGGGCTACTGCTTGTAGCTTTGGTGGTTCTCGACCGTTTGTGGTAGCAGTAGATGACAT
CATGTTTCAGAAACCTGTTGAGGTTGGCTCATTGCTCTTTCTTTCTTCACAGGTATGCTT
TACTCAGAATAATTATATTCAAGTCAGAGTACACAGTGAAGTGGCCTCCCTGCAGGAGAA
GCAGCATACAACCACCAATGTCTTTCATTTCACGTTCATGTCGGAAAAAGAAGTGCCATT
GGTTTTCCCAAAAACATATGGAGAGTCCATGTTGTACTTAGATGGGCAGCGGCATTTCAA
CTCCATGAGTGGCCCAGCGACCTTGAGAAAGGACTACCTTGTGGAGCCCGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT